Although the taxonomy of oligotrich ciliates has been widely investigated,yet the species diversity remains poorly known.We newly designed a pair of oligotrich-specific LSU rDNA primers covering the 600-bp D1/D2 regio...Although the taxonomy of oligotrich ciliates has been widely investigated,yet the species diversity remains poorly known.We newly designed a pair of oligotrich-specific LSU rDNA primers covering the 600-bp D1/D2 region,and it was effective for detecting oligotrich species.Using the primers,we constructed the cloning libraries to investigate the species diversity of oligotrichs in the northern coastal waters of the South China Sea.In total,165 oligotrich sequences were obtained from five widely separated sampling sites.Sixty operational taxonomic units(OTUs)were obtained at 99%similarity threshold,and low-abundance OTUs with no more than two sequences contributed most of these(about 78%).Our findings are consistent with previous morphological studies,Strombidium was found the most abundant and widely distributed genus in this area.In addition,the BLAST search in the NCBI database resulted in 95%OTUs matching with named oligotrich species in similarity below 99%.Therefore,oligotrich morphospecies diversity has been underestimated as low-abundance species,and the LSU rDNA oligotrich sequence database needs to be better promoted.展开更多
分析老年福祉技术研究中的学科交叉现象,为我国老年福址技术创新研究提供参考。借助社会网络分析方法,对Web of Science收录的1945—2018年老年福祉技术研究文献数据进行学科交叉主题与特征分析。研究发现,老年福祉技术研究领域存在多...分析老年福祉技术研究中的学科交叉现象,为我国老年福址技术创新研究提供参考。借助社会网络分析方法,对Web of Science收录的1945—2018年老年福祉技术研究文献数据进行学科交叉主题与特征分析。研究发现,老年福祉技术研究领域存在多学科交叉特点。临床医学、公共卫生与预防医学、计算机科学与技术、社会学等学科在老年福祉技术研究中占据重要地位,是学科间联系的主要媒介,其中临床医学交叉程度最高。阿尔茨海默病和老年辅助技术是老年福祉技术研究的关键。展开更多
对老龄科技研究进行系统性梳理,对其发展脉络进行阶段性划分,并运用可视化软件构建了知识图谱。CiteSpaceⅡ软件的运用,使老龄科技研究的相关引文结构和历史文献得以可视化。研究发现:最近二十年是老龄科技研究获得广泛关注的阶段;老龄...对老龄科技研究进行系统性梳理,对其发展脉络进行阶段性划分,并运用可视化软件构建了知识图谱。CiteSpaceⅡ软件的运用,使老龄科技研究的相关引文结构和历史文献得以可视化。研究发现:最近二十年是老龄科技研究获得广泛关注的阶段;老龄科技的热点研究领域覆盖了信息与通信技术(information and communication technology)和智慧家庭(smart home)等多个方向;老龄科技研究领域未来的发展方向正在向居家养老(aging in place)和智慧家庭转变。展开更多
基金Supported by the National Natural Science Foundation of China(Nos.31772440,31430077,41576124,31761133001)the Pearl River Science and Technology Nova Program of Guangzhou(No.201610010162) to YZ+1 种基金Guangdong MEPP Fund(No.GDOE(2019)A23)the Special Support Program of Guangdong Province to YZ
文摘Although the taxonomy of oligotrich ciliates has been widely investigated,yet the species diversity remains poorly known.We newly designed a pair of oligotrich-specific LSU rDNA primers covering the 600-bp D1/D2 region,and it was effective for detecting oligotrich species.Using the primers,we constructed the cloning libraries to investigate the species diversity of oligotrichs in the northern coastal waters of the South China Sea.In total,165 oligotrich sequences were obtained from five widely separated sampling sites.Sixty operational taxonomic units(OTUs)were obtained at 99%similarity threshold,and low-abundance OTUs with no more than two sequences contributed most of these(about 78%).Our findings are consistent with previous morphological studies,Strombidium was found the most abundant and widely distributed genus in this area.In addition,the BLAST search in the NCBI database resulted in 95%OTUs matching with named oligotrich species in similarity below 99%.Therefore,oligotrich morphospecies diversity has been underestimated as low-abundance species,and the LSU rDNA oligotrich sequence database needs to be better promoted.
文摘分析老年福祉技术研究中的学科交叉现象,为我国老年福址技术创新研究提供参考。借助社会网络分析方法,对Web of Science收录的1945—2018年老年福祉技术研究文献数据进行学科交叉主题与特征分析。研究发现,老年福祉技术研究领域存在多学科交叉特点。临床医学、公共卫生与预防医学、计算机科学与技术、社会学等学科在老年福祉技术研究中占据重要地位,是学科间联系的主要媒介,其中临床医学交叉程度最高。阿尔茨海默病和老年辅助技术是老年福祉技术研究的关键。
文摘对老龄科技研究进行系统性梳理,对其发展脉络进行阶段性划分,并运用可视化软件构建了知识图谱。CiteSpaceⅡ软件的运用,使老龄科技研究的相关引文结构和历史文献得以可视化。研究发现:最近二十年是老龄科技研究获得广泛关注的阶段;老龄科技的热点研究领域覆盖了信息与通信技术(information and communication technology)和智慧家庭(smart home)等多个方向;老龄科技研究领域未来的发展方向正在向居家养老(aging in place)和智慧家庭转变。
