Indicator systems of environmental sustainable development in the Poyang Lake Basin are established from 51 elementary indexes by factor analysis, which is composed of four steps such as the factor model, the paramete...Indicator systems of environmental sustainable development in the Poyang Lake Basin are established from 51 elementary indexes by factor analysis, which is composed of four steps such as the factor model, the parameter estimation, the factor rotation and the factor score. Under the condition that the cumulative proportion is greater than 85%, 5 explicit factors of environmental sustainable development as well as its factor score by region are carried out. The result indicates some impact factors to the basin environmental in descending sort order are volume of water, volume of waste gas discharge,volume of solid wasters.the degree to comprehensive utilization of waste gas, waste water and solid wastes, the emission volume of waste gas, waste water and solid wastes. It is helpful and important to provide decision support for constituting sustainable development strategies and evaluate the sustainable development status of each city.展开更多
小麦矮缩病毒(wheat dwarf virus,WDV)引起的小麦矮缩病是一种危害严重的小麦病害。为研究小麦与WDV的互作机制,本研究首先以小麦为供试材料,提取小麦幼苗叶片和根组织的总RNA,采用SMART(switching mechanism at 5′end of RNA transcri...小麦矮缩病毒(wheat dwarf virus,WDV)引起的小麦矮缩病是一种危害严重的小麦病害。为研究小麦与WDV的互作机制,本研究首先以小麦为供试材料,提取小麦幼苗叶片和根组织的总RNA,采用SMART(switching mechanism at 5′end of RNA transcript)技术构建了小麦全长cDNA文库。该文库滴度大于4×10^(9)cfu/mL、初级文库库容量超过3.6×10^(6)cfu,插入片段长度在500~2000 bp之间,平均长度大于1000 bp,重组率约100%,成功获得了高质量的小麦cDNA文库。为筛选与WDV复制蛋白(replication protein,Rep)互作的寄主因子,构建了酵母双杂交诱饵载体pGBKT7-Rep,验证其无毒性和自激活现象后,利用构建的文库初步筛选出32个与WDV-Rep蛋白发生相互作用的候选靶标蛋白。在此基础上,采用酵母双杂交系统对筛选获得的10个候选蛋白进行一对一互作验证,最终确定其中8个蛋白与WDV-Rep存在相互作用。生物信息学分析表明,这些阳性互作蛋白包括锌指转录因子TFⅢA、去SUMO化酶DeSI、E3泛素连接酶BRE1等,其功能涉及基因转录调控、蛋白质泛素化修饰、植物抗逆防御应答、光合作用能量转换和RNA加工等多个关键生物学过程。该研究为深入解析WDV致病机理和寄主抗病机制提供了材料和理论基础。展开更多
文摘Indicator systems of environmental sustainable development in the Poyang Lake Basin are established from 51 elementary indexes by factor analysis, which is composed of four steps such as the factor model, the parameter estimation, the factor rotation and the factor score. Under the condition that the cumulative proportion is greater than 85%, 5 explicit factors of environmental sustainable development as well as its factor score by region are carried out. The result indicates some impact factors to the basin environmental in descending sort order are volume of water, volume of waste gas discharge,volume of solid wasters.the degree to comprehensive utilization of waste gas, waste water and solid wastes, the emission volume of waste gas, waste water and solid wastes. It is helpful and important to provide decision support for constituting sustainable development strategies and evaluate the sustainable development status of each city.
