Transgenic tobacco(Nicotiana tabacum L.cv.Xanthi)plants which express coat protein(CP)gene(cp)of potato virus X(PVX)were generated via Agrobacterium tumefaciens-mediated gene transfer.Northern blotting analysis indica...Transgenic tobacco(Nicotiana tabacum L.cv.Xanthi)plants which express coat protein(CP)gene(cp)of potato virus X(PVX)were generated via Agrobacterium tumefaciens-mediated gene transfer.Northern blotting analysis indicated that cp silencing happened in three transformants,and Run on assay showed that the cp silencing was at post-transcriptional level.The result of cp methylation assay showed different degree of methylation existed in coat protein gene coding sequences.cp methylation was also analyzed during virus-induced gene silencing(VIGS),and results suggested that methylation pre-existed in cp non-silenced transgenic plants,while virus infection increase the methylation degree,which indicated DNA methylation in VIGS was not a de novo DNA methylation process.展开更多
文摘Transgenic tobacco(Nicotiana tabacum L.cv.Xanthi)plants which express coat protein(CP)gene(cp)of potato virus X(PVX)were generated via Agrobacterium tumefaciens-mediated gene transfer.Northern blotting analysis indicated that cp silencing happened in three transformants,and Run on assay showed that the cp silencing was at post-transcriptional level.The result of cp methylation assay showed different degree of methylation existed in coat protein gene coding sequences.cp methylation was also analyzed during virus-induced gene silencing(VIGS),and results suggested that methylation pre-existed in cp non-silenced transgenic plants,while virus infection increase the methylation degree,which indicated DNA methylation in VIGS was not a de novo DNA methylation process.
