三七药材质量影响其临床治疗效果。目前三七质量参差不齐,其质量控制主要依赖于皂苷类化合物,无法系统地反映三七药材品质。本研究基于不同产区三七药材的收集,采用微量法、紫外-可见分光光度法、高效液相色谱法定量分析影响三七功效及...三七药材质量影响其临床治疗效果。目前三七质量参差不齐,其质量控制主要依赖于皂苷类化合物,无法系统地反映三七药材品质。本研究基于不同产区三七药材的收集,采用微量法、紫外-可见分光光度法、高效液相色谱法定量分析影响三七功效及剂型的多维度指标。结果表明,三七中大分子物质(淀粉、纤维素、木质素)含量较高的产区为云南曲靖沾益炎方乡,总多糖含量较高的产区为云南文山州麻栗坡麻栗镇,总黄酮含量较高的产地为云南省玉溪华宁青龙镇,三七素含量较高的产地为云南昆明寻甸甸沙乡,总皂苷含量较高的产地为云南文山州麻栗坡麻栗镇。基于层次聚类和正交偏最小二乘法判别分析(orthogonal partial least squares-discrimination analysis,OPLS-DA),确定了三七素、总多糖、支链淀粉、纤维素可以作为区分不同产区三七药材的潜在生物标志物。利用药用植物全球产地生态适宜性区划信息系统获取三七产区的19个生态因子,结合相关性分析确定了生态因子对三七各成分积累的影响。其中,年降水量、等温性、平均月温差、最冷月份最低温度、最暖季度平均温度、最湿季度降水量以及最暖季度降水量是影响三七质量的关键生态因子。综上,本研究全面评价了不同产地三七质量,揭示了影响三七道地性品质的关键生态因子,为后续三七质量控制和合理选址提供了参考依据。展开更多
重楼皂苷是中药重楼主要的有效成分,为了快速评价重楼品质,保证重楼在临床治疗中的疗效,本文采用红外光谱结合偏最小二乘回归法(partial least squares regression,PLSR)对重楼中重楼皂苷Ⅰ、重楼皂苷Ⅱ和重楼皂苷Ⅶ进行定量分析,建立...重楼皂苷是中药重楼主要的有效成分,为了快速评价重楼品质,保证重楼在临床治疗中的疗效,本文采用红外光谱结合偏最小二乘回归法(partial least squares regression,PLSR)对重楼中重楼皂苷Ⅰ、重楼皂苷Ⅱ和重楼皂苷Ⅶ进行定量分析,建立快速评价重楼品质的方法。采集78份不同产区、不同种类重楼样品的红外光谱,用高效液相色谱测定重楼样品中重楼皂苷Ⅰ、重楼皂苷Ⅱ及重楼皂苷Ⅶ的含量,将重楼的红外光谱数据和液相数据进行拟合,快速预测3种重楼皂苷含量。原始红外光谱经多元散射校正(multiplicative signal correction,MSC)、标准正态变量(standard normal variate,SNV)、正交信号校正(orthogonal signal correction,OSC)、一阶求导(first derivative,1st Der)、二阶求导(second derivative,2nd Der)预处理后,运用偏最小二乘回归分析建立重楼皂苷的定量预测模型。重楼皂苷Ⅰ和重楼皂苷Ⅱ的最佳预处理方法为MSC+OSC+2nd Der,重楼皂苷Ⅶ的最佳预处理方法为MSC+SNV+OSC+2nd Der;重楼皂苷Ⅰ、重楼皂苷Ⅱ和重楼皂苷Ⅶ3个指标成分定量校正模型的决定系数(R^2)分别为0.9308,0.9348,0.9123 mg·g^(-1);校正均方根误差(root mean square error of estimation,RMSEE)分别为1.8550,0.6323,0.0016 mg·g^(-1);验证模型的决定系数(R^2)分别为0.9488,0.9636,0.7801 mg·g^(-1);预测均方根误差(root mean square error of prediction,RMSEP)分别为1.7046,1.2277,0.0019 mg·g^(-1);定量模型的预测值与真实值比较接近,模型预测效果好,其中重楼皂苷Ⅰ,Ⅱ定量模型效果优于重楼皂苷Ⅶ。该方法无损、快速、准确,可用于重楼中重楼皂苷含量的快速测定。展开更多
The genetic diversity of the 34 populations of wild rice Oryza meyeriana Baill. distributed in Yunnan Province, China was analyzed using 13 inter-simple sequence repeat （ISSR） markers. A total of 168 bands were ampl...The genetic diversity of the 34 populations of wild rice Oryza meyeriana Baill. distributed in Yunnan Province, China was analyzed using 13 inter-simple sequence repeat （ISSR） markers. A total of 168 bands were amplified, of which 135 polymorphic bands were discovered and the percentage of polymorphic bands （PPB） was 80.36%. A genetic diversity was revealed as Nei＇s gene diversity （H） = 0.2666 and Shannon information index （I） = 0.4028 at population level. The 34 populations were divided into different groups based on administrative regions, latitude and longitudes, river areas, altitudes of their origins, and their indexes such as Na （number of alleles）, Ne （effective number of alleles）, H, I and PPB were calculated. Richer genetic diversity was found in the wild rice populations distributed in Simao Prefecture than that in Lingcang Prefecture or Xishuangbanna Prefecture whereas the least genetic diversity was in Baoshan Prefecture or Dehong Prefecture. Rich genetic diversity was also discovered in the wild rice populations originated from higher than 710 m altitude around the middle and lower reaches of the Lancang River belonging to the Pacific Ocean drainage system. The 34 populations could be classified into two groups, one group covered the wild rice distributing in Simao Prefecture only while the other group covered ones in Lingcang, Xishuangbanna and Dehong Prefectures. The issue on how to effectively conserve the wild rice germplasm was discussed.展开更多
文摘三七药材质量影响其临床治疗效果。目前三七质量参差不齐,其质量控制主要依赖于皂苷类化合物,无法系统地反映三七药材品质。本研究基于不同产区三七药材的收集,采用微量法、紫外-可见分光光度法、高效液相色谱法定量分析影响三七功效及剂型的多维度指标。结果表明,三七中大分子物质(淀粉、纤维素、木质素)含量较高的产区为云南曲靖沾益炎方乡,总多糖含量较高的产区为云南文山州麻栗坡麻栗镇,总黄酮含量较高的产地为云南省玉溪华宁青龙镇,三七素含量较高的产地为云南昆明寻甸甸沙乡,总皂苷含量较高的产地为云南文山州麻栗坡麻栗镇。基于层次聚类和正交偏最小二乘法判别分析(orthogonal partial least squares-discrimination analysis,OPLS-DA),确定了三七素、总多糖、支链淀粉、纤维素可以作为区分不同产区三七药材的潜在生物标志物。利用药用植物全球产地生态适宜性区划信息系统获取三七产区的19个生态因子,结合相关性分析确定了生态因子对三七各成分积累的影响。其中,年降水量、等温性、平均月温差、最冷月份最低温度、最暖季度平均温度、最湿季度降水量以及最暖季度降水量是影响三七质量的关键生态因子。综上,本研究全面评价了不同产地三七质量,揭示了影响三七道地性品质的关键生态因子,为后续三七质量控制和合理选址提供了参考依据。
文摘重楼皂苷是中药重楼主要的有效成分,为了快速评价重楼品质,保证重楼在临床治疗中的疗效,本文采用红外光谱结合偏最小二乘回归法(partial least squares regression,PLSR)对重楼中重楼皂苷Ⅰ、重楼皂苷Ⅱ和重楼皂苷Ⅶ进行定量分析,建立快速评价重楼品质的方法。采集78份不同产区、不同种类重楼样品的红外光谱,用高效液相色谱测定重楼样品中重楼皂苷Ⅰ、重楼皂苷Ⅱ及重楼皂苷Ⅶ的含量,将重楼的红外光谱数据和液相数据进行拟合,快速预测3种重楼皂苷含量。原始红外光谱经多元散射校正(multiplicative signal correction,MSC)、标准正态变量(standard normal variate,SNV)、正交信号校正(orthogonal signal correction,OSC)、一阶求导(first derivative,1st Der)、二阶求导(second derivative,2nd Der)预处理后,运用偏最小二乘回归分析建立重楼皂苷的定量预测模型。重楼皂苷Ⅰ和重楼皂苷Ⅱ的最佳预处理方法为MSC+OSC+2nd Der,重楼皂苷Ⅶ的最佳预处理方法为MSC+SNV+OSC+2nd Der;重楼皂苷Ⅰ、重楼皂苷Ⅱ和重楼皂苷Ⅶ3个指标成分定量校正模型的决定系数(R^2)分别为0.9308,0.9348,0.9123 mg·g^(-1);校正均方根误差(root mean square error of estimation,RMSEE)分别为1.8550,0.6323,0.0016 mg·g^(-1);验证模型的决定系数(R^2)分别为0.9488,0.9636,0.7801 mg·g^(-1);预测均方根误差(root mean square error of prediction,RMSEP)分别为1.7046,1.2277,0.0019 mg·g^(-1);定量模型的预测值与真实值比较接近,模型预测效果好,其中重楼皂苷Ⅰ,Ⅱ定量模型效果优于重楼皂苷Ⅶ。该方法无损、快速、准确,可用于重楼中重楼皂苷含量的快速测定。
基金This work was supported by the National Natural Science Foundation of China(Grant No.30460065)Yunnan Provincial Foundation for Science.
文摘The genetic diversity of the 34 populations of wild rice Oryza meyeriana Baill. distributed in Yunnan Province, China was analyzed using 13 inter-simple sequence repeat （ISSR） markers. A total of 168 bands were amplified, of which 135 polymorphic bands were discovered and the percentage of polymorphic bands （PPB） was 80.36%. A genetic diversity was revealed as Nei＇s gene diversity （H） = 0.2666 and Shannon information index （I） = 0.4028 at population level. The 34 populations were divided into different groups based on administrative regions, latitude and longitudes, river areas, altitudes of their origins, and their indexes such as Na （number of alleles）, Ne （effective number of alleles）, H, I and PPB were calculated. Richer genetic diversity was found in the wild rice populations distributed in Simao Prefecture than that in Lingcang Prefecture or Xishuangbanna Prefecture whereas the least genetic diversity was in Baoshan Prefecture or Dehong Prefecture. Rich genetic diversity was also discovered in the wild rice populations originated from higher than 710 m altitude around the middle and lower reaches of the Lancang River belonging to the Pacific Ocean drainage system. The 34 populations could be classified into two groups, one group covered the wild rice distributing in Simao Prefecture only while the other group covered ones in Lingcang, Xishuangbanna and Dehong Prefectures. The issue on how to effectively conserve the wild rice germplasm was discussed.
