Studies of herpes simplex virus type 1 （HSV-1） infection have shown that many known and unknown cellular molecules in- volved in viral proliferation are up-regulated following HSV-1 infection. In this study, using t...Studies of herpes simplex virus type 1 （HSV-1） infection have shown that many known and unknown cellular molecules in- volved in viral proliferation are up-regulated following HSV-1 infection. In this study, using two-dimensional polyacrylamide gel electrophoresis, we found that the expression of the HSV-1 infection response repressive protein （HIRRP, GI 16552881） was up-regulated in human L02 cells infected with HSV-1. HIRRP, an unknown protein, was initially localized in the cytoplasm and then translocated into the nucleus of HSV-l-infected cells. Further analysis showed that HIRRP represses HSV-1 proliferation by inhibiting transcription of the viral genome by interacting with the cellular transcription factor, ATFS, via its N-terminal domain. ATF5 represses the transcription of many host genes but can also act as an activator of genes containing a specific motif. We found that ATF5 promotes the proliferation of HSV-1 via a potential mechanism by which ATF5 enhances the transcription of viral genes during the course of an HSV-1 infection; HIRRP then induces feedback repression of this tran- scription by interacting with ATFS.展开更多
基金supported by the National Basic Research Program of China(2012CB518901)the National Natural Science Foundation of China(31100127)
文摘Studies of herpes simplex virus type 1 （HSV-1） infection have shown that many known and unknown cellular molecules in- volved in viral proliferation are up-regulated following HSV-1 infection. In this study, using two-dimensional polyacrylamide gel electrophoresis, we found that the expression of the HSV-1 infection response repressive protein （HIRRP, GI 16552881） was up-regulated in human L02 cells infected with HSV-1. HIRRP, an unknown protein, was initially localized in the cytoplasm and then translocated into the nucleus of HSV-l-infected cells. Further analysis showed that HIRRP represses HSV-1 proliferation by inhibiting transcription of the viral genome by interacting with the cellular transcription factor, ATFS, via its N-terminal domain. ATF5 represses the transcription of many host genes but can also act as an activator of genes containing a specific motif. We found that ATF5 promotes the proliferation of HSV-1 via a potential mechanism by which ATF5 enhances the transcription of viral genes during the course of an HSV-1 infection; HIRRP then induces feedback repression of this tran- scription by interacting with ATFS.
