Quantitative structure-retention relationship(QSRR)is an important tool in chromatography.QSRR examines the correlation between molecular structures and their retention behaviors during chromatographic separation.This...Quantitative structure-retention relationship(QSRR)is an important tool in chromatography.QSRR examines the correlation between molecular structures and their retention behaviors during chromatographic separation.This approach involves developing models for predicting the retention time(RT)of analytes,thereby accelerating method development and facilitating compound identification.In addition,QSRR can be used to study compound retention mechanisms and support drug screening efforts.This review provides a comprehensive analysis of QSRR workflows and applications,with a special focus on the role of artificial intelligence-an area not thoroughly explored in previous reviews.Moreover,we discuss current limitations in RT prediction and propose promising solutions.Overall,this review offers a fresh perspective on future QSRR research,encouraging the development of innovative strategies that enable the diverse applications of QSRR models in chromatographic analysis.展开更多
Study on landscape spatial pattern in the center of Chengdu City is based on the Landsat-8 image by using 3S technique and spatial pattern analysis software Fragstats 3.3. The results showed that there covered an area...Study on landscape spatial pattern in the center of Chengdu City is based on the Landsat-8 image by using 3S technique and spatial pattern analysis software Fragstats 3.3. The results showed that there covered an area of 193.92 km<sup>2</sup> in the center of Chengdu City. 55.24% of the total area was made up of buildings, covering an area of 107.12 km<sup>2</sup>, and the overall landscape of impervious ground-based. There were 22,666 patches;29.67% of the patches were roads. The human interference of buildings, roads and green space was a maximum;fragmentation of the highest was green space. The fragmentation of overall urban landscape was high;the diversity was low;the value of Shannon’s diversity index was 0.63;the value of Shannon’s evenness index was 0.70. All kinds of landscape distribution were not balanced, and lack of connectivity. This research puts important reference for creating good urban landscape space and restoring the natural ecological environment in the western regions of China.展开更多
Objective:This study aimed to assess the effects of electroacupuncture(EA)at the contralateral,ipsilateral,or bilateral"Zusanli(ST36)"and"Yanglingquan(GB34)"on neuropathic pain caused by chronic co...Objective:This study aimed to assess the effects of electroacupuncture(EA)at the contralateral,ipsilateral,or bilateral"Zusanli(ST36)"and"Yanglingquan(GB34)"on neuropathic pain caused by chronic contractile injury(CCI)and to explore the role of the nuclear factor erythroid 2-related factor 2(Nrf2)pathway in the effects of EA.Methods:Male Sprague-Dawley rats were subjected to the CCI model to induce neuropathic pain.A total of 45 rats were randomly divided into five groups(n=9):sham,CCI,EA-Co(CCI+EA at contralateral acupoints),EA-Ip(CCI+EA at ipsilateral acupoints),and EA-Bi(CCI+EA at bilateral acupoints).The rats received EA treatment on day 8 after CCI,once every alternate day,for a total of eight times.The time courses of mechanical pain threshold(MWT),hind paw withdrawal latency(HWL),and sciatic functional index(SFI)were determined.The expression levels of 8-hydroxydeoxyguanosine(8-OHdG),glutathione(GSH),superoxide dismutase(SOD)activity,interleukin-6(IL-6),interleukin-1 beta(IL-1β),and tumor necrosis factor factor-alpha(TNF-α)in the spinal cord were measured.The distribution of Nrf2,its expression of Nrf2 in both the cytosol and nucleus,and the protein levels of its downstream target genes,NQO1 and HO-1,were detected via double immunofluorescence staining and western blotting,respectively.Results:Following CCI,both MWT and HWL in the CCI group significantly decreased from day 14 after surgery(P<0.001).EA treatment exhibited significant antinociceptive effects induced by CCI by increasing the MWT and HWL values,especially bilateral EA(P<0.05).The SFI of the CCI group was significantly lower than that of the sham group(P<0.001).Only bilateral EA improved the SFI scores compared to the CCI group(P<0.05).8-OHdG levels in the spinal cord of the CCI group were significantly higher than those in the sham group(P<0.05),whereas GSH levels and SOD activity in the spinal cord of the CCI group were significantly lower than those in the sham group(P<0.001 and P<0.01,respectively).Bilateral EA administration significantly downregulated 8-OHdG levels(P<0.01)and upregulated GSH levels and SOD activity in the spinal cord(P<0.01).CCI significantly enhanced the production of IL-1β,IL-6,and TNF-αin the spinal cord compared with that in the sham group(all P<0.001).Meanwhile,the effects of EA were also accompanied by markedly decreased expression of IL-1βand IL-6 in the spinal cord(P<0.05).TNF-αlevels were only decreased in the EA-Ip and EA-Bi groups compared with those in the CCI group(P<0.001).Confocal microscopy revealed that Nrf2 was mainly localized in the neurons of the spinal cord.Notably,EA treatment enhanced nuclear translocation of Nrf2 in neurons.CCI significantly decreased the production of Nrf2,HO-1,and NQO1 in the spinal cord compared to the sham group(P<0.001),and bilateral EA up-regulated the protein levels of Nrf2 and its target genes HO-1 and NQO1(all P<0.001).Conclusion:Our results suggest that bilateral EA is an optimal therapeutic strategy for neuropathic pain.The effects of EA on neuropathic pain may be mediated by the restoration of the Nrf2 pathway in the spinal cord.展开更多
目的观察和分析左心瓣膜疾病相关性肺高压(pulmonary artery hypertension due to left heart valve diseases,LHD-PAH)的临床特征、危险因素和对临床结局的影响。方法纳入2012年1月至2015年2 月在我科行体外循环心脏瓣膜手术的患者。...目的观察和分析左心瓣膜疾病相关性肺高压(pulmonary artery hypertension due to left heart valve diseases,LHD-PAH)的临床特征、危险因素和对临床结局的影响。方法纳入2012年1月至2015年2 月在我科行体外循环心脏瓣膜手术的患者。以超声心动图为主要诊断标准,LHD-PAH采用三尖瓣反流法估算的肺动脉收缩压值进行诊断。收集患者的临床信息进行统计分析。结果共纳入958例患者,其中合并LHD-PAH的患者共391例,占40.8%。通过单因素分析及多因素Logistic回归分析,我们发现射血分数<30%(OR=4.490,95%CI 1.920-10.499,P<0.01)、心房颤动(OR=9.654,95%CI 3.214-21.411 ,P<0.01)、左心房血栓(OR=3.991,95%CI 2.946-5.406,P<0.01)为LHD-PAH的独立危险因素。一旦左心瓣膜疾病患者出现 LHD-PAH后,其体外循环转流时间将会显著高于非LHD-PAH患者(P<0.01),然而主动脉阻断时间则无显著差异(P=0.331)o而且,LHD-PAH的出现还会增加患者的机械通气时间、ICU留治时间、总住院日以及病死率(P<0.01)。结论LHD-PAH在临床中发生率较高,患者临床结局不良,射血分数<30%、心房颤动和左心房血栓是其独立危险因素。展开更多
Circular RNAs can regulate the development and progression of ischemic cerebral disease.However,it remains unclear whether they play a role in acute ischemic stroke.To investigate the role of the circular RNA Rap1b(ci...Circular RNAs can regulate the development and progression of ischemic cerebral disease.However,it remains unclear whether they play a role in acute ischemic stroke.To investigate the role of the circular RNA Rap1b(circRap1b)in acute ischemic stroke,in this study we established an in vitro model of acute ischemia and hypoxia by subjecting HT22 cells to oxygen and glucose deprivation and a mouse model of acute ischemia and hypoxia by occluding the right carotid artery.We found that circRap1b expression was remarkably down-regulated in the hippocampal tissue of the mouse model and in the HT22 cell model.In addition,Hoxa5 expression was strongly up-regulated in response to circRap1b overexpression.Hoxa5 expression was low in the hippocampus of a mouse model of acute ischemia and in HT22-AIS cells,and inhibited HT22-AIS cell apoptosis.Importantly,we found that circRap1b promoted Hoxa5 transcription by recruiting the acetyltransferase Kat7 to induce H3K14ac modification in the Hoxa5 promoter region.Hoxa5 regulated neuronal apoptosis by activating transcription of Fam3a,a neuronal apoptosis-related protein.These results suggest that circRap1b regulates Hoxa5 transcription and expression,and subsequently Fam3a expression,ultimately inhibiting cell apoptosis.Lastly,we explored the potential clinical relevance of circRap1b and Hoxa5 in vivo.Taken together,these findings demonstrate the mechanism by which circRap1b inhibits neuronal apoptosis in acute ischemic stroke.展开更多
基金supported by the Shanghai Sailing Program,China(Grant No.:23YF1413300).
文摘Quantitative structure-retention relationship(QSRR)is an important tool in chromatography.QSRR examines the correlation between molecular structures and their retention behaviors during chromatographic separation.This approach involves developing models for predicting the retention time(RT)of analytes,thereby accelerating method development and facilitating compound identification.In addition,QSRR can be used to study compound retention mechanisms and support drug screening efforts.This review provides a comprehensive analysis of QSRR workflows and applications,with a special focus on the role of artificial intelligence-an area not thoroughly explored in previous reviews.Moreover,we discuss current limitations in RT prediction and propose promising solutions.Overall,this review offers a fresh perspective on future QSRR research,encouraging the development of innovative strategies that enable the diverse applications of QSRR models in chromatographic analysis.
文摘Study on landscape spatial pattern in the center of Chengdu City is based on the Landsat-8 image by using 3S technique and spatial pattern analysis software Fragstats 3.3. The results showed that there covered an area of 193.92 km<sup>2</sup> in the center of Chengdu City. 55.24% of the total area was made up of buildings, covering an area of 107.12 km<sup>2</sup>, and the overall landscape of impervious ground-based. There were 22,666 patches;29.67% of the patches were roads. The human interference of buildings, roads and green space was a maximum;fragmentation of the highest was green space. The fragmentation of overall urban landscape was high;the diversity was low;the value of Shannon’s diversity index was 0.63;the value of Shannon’s evenness index was 0.70. All kinds of landscape distribution were not balanced, and lack of connectivity. This research puts important reference for creating good urban landscape space and restoring the natural ecological environment in the western regions of China.
基金Supported by National Natural Science Foundation of China:81973940Shanghai Clinical Research Center for Acupuncture and Moxibustion:20MC1920500Clinical Key Specialty Construction Foundation of Shanghai:shslczdzk04701。
文摘Objective:This study aimed to assess the effects of electroacupuncture(EA)at the contralateral,ipsilateral,or bilateral"Zusanli(ST36)"and"Yanglingquan(GB34)"on neuropathic pain caused by chronic contractile injury(CCI)and to explore the role of the nuclear factor erythroid 2-related factor 2(Nrf2)pathway in the effects of EA.Methods:Male Sprague-Dawley rats were subjected to the CCI model to induce neuropathic pain.A total of 45 rats were randomly divided into five groups(n=9):sham,CCI,EA-Co(CCI+EA at contralateral acupoints),EA-Ip(CCI+EA at ipsilateral acupoints),and EA-Bi(CCI+EA at bilateral acupoints).The rats received EA treatment on day 8 after CCI,once every alternate day,for a total of eight times.The time courses of mechanical pain threshold(MWT),hind paw withdrawal latency(HWL),and sciatic functional index(SFI)were determined.The expression levels of 8-hydroxydeoxyguanosine(8-OHdG),glutathione(GSH),superoxide dismutase(SOD)activity,interleukin-6(IL-6),interleukin-1 beta(IL-1β),and tumor necrosis factor factor-alpha(TNF-α)in the spinal cord were measured.The distribution of Nrf2,its expression of Nrf2 in both the cytosol and nucleus,and the protein levels of its downstream target genes,NQO1 and HO-1,were detected via double immunofluorescence staining and western blotting,respectively.Results:Following CCI,both MWT and HWL in the CCI group significantly decreased from day 14 after surgery(P<0.001).EA treatment exhibited significant antinociceptive effects induced by CCI by increasing the MWT and HWL values,especially bilateral EA(P<0.05).The SFI of the CCI group was significantly lower than that of the sham group(P<0.001).Only bilateral EA improved the SFI scores compared to the CCI group(P<0.05).8-OHdG levels in the spinal cord of the CCI group were significantly higher than those in the sham group(P<0.05),whereas GSH levels and SOD activity in the spinal cord of the CCI group were significantly lower than those in the sham group(P<0.001 and P<0.01,respectively).Bilateral EA administration significantly downregulated 8-OHdG levels(P<0.01)and upregulated GSH levels and SOD activity in the spinal cord(P<0.01).CCI significantly enhanced the production of IL-1β,IL-6,and TNF-αin the spinal cord compared with that in the sham group(all P<0.001).Meanwhile,the effects of EA were also accompanied by markedly decreased expression of IL-1βand IL-6 in the spinal cord(P<0.05).TNF-αlevels were only decreased in the EA-Ip and EA-Bi groups compared with those in the CCI group(P<0.001).Confocal microscopy revealed that Nrf2 was mainly localized in the neurons of the spinal cord.Notably,EA treatment enhanced nuclear translocation of Nrf2 in neurons.CCI significantly decreased the production of Nrf2,HO-1,and NQO1 in the spinal cord compared to the sham group(P<0.001),and bilateral EA up-regulated the protein levels of Nrf2 and its target genes HO-1 and NQO1(all P<0.001).Conclusion:Our results suggest that bilateral EA is an optimal therapeutic strategy for neuropathic pain.The effects of EA on neuropathic pain may be mediated by the restoration of the Nrf2 pathway in the spinal cord.
文摘目的观察和分析左心瓣膜疾病相关性肺高压(pulmonary artery hypertension due to left heart valve diseases,LHD-PAH)的临床特征、危险因素和对临床结局的影响。方法纳入2012年1月至2015年2 月在我科行体外循环心脏瓣膜手术的患者。以超声心动图为主要诊断标准,LHD-PAH采用三尖瓣反流法估算的肺动脉收缩压值进行诊断。收集患者的临床信息进行统计分析。结果共纳入958例患者,其中合并LHD-PAH的患者共391例,占40.8%。通过单因素分析及多因素Logistic回归分析,我们发现射血分数<30%(OR=4.490,95%CI 1.920-10.499,P<0.01)、心房颤动(OR=9.654,95%CI 3.214-21.411 ,P<0.01)、左心房血栓(OR=3.991,95%CI 2.946-5.406,P<0.01)为LHD-PAH的独立危险因素。一旦左心瓣膜疾病患者出现 LHD-PAH后,其体外循环转流时间将会显著高于非LHD-PAH患者(P<0.01),然而主动脉阻断时间则无显著差异(P=0.331)o而且,LHD-PAH的出现还会增加患者的机械通气时间、ICU留治时间、总住院日以及病死率(P<0.01)。结论LHD-PAH在临床中发生率较高,患者临床结局不良,射血分数<30%、心房颤动和左心房血栓是其独立危险因素。
基金supported by the Natural Science Foundation of Liaoning Province,No.2021-MS-061(to LZhang)。
文摘Circular RNAs can regulate the development and progression of ischemic cerebral disease.However,it remains unclear whether they play a role in acute ischemic stroke.To investigate the role of the circular RNA Rap1b(circRap1b)in acute ischemic stroke,in this study we established an in vitro model of acute ischemia and hypoxia by subjecting HT22 cells to oxygen and glucose deprivation and a mouse model of acute ischemia and hypoxia by occluding the right carotid artery.We found that circRap1b expression was remarkably down-regulated in the hippocampal tissue of the mouse model and in the HT22 cell model.In addition,Hoxa5 expression was strongly up-regulated in response to circRap1b overexpression.Hoxa5 expression was low in the hippocampus of a mouse model of acute ischemia and in HT22-AIS cells,and inhibited HT22-AIS cell apoptosis.Importantly,we found that circRap1b promoted Hoxa5 transcription by recruiting the acetyltransferase Kat7 to induce H3K14ac modification in the Hoxa5 promoter region.Hoxa5 regulated neuronal apoptosis by activating transcription of Fam3a,a neuronal apoptosis-related protein.These results suggest that circRap1b regulates Hoxa5 transcription and expression,and subsequently Fam3a expression,ultimately inhibiting cell apoptosis.Lastly,we explored the potential clinical relevance of circRap1b and Hoxa5 in vivo.Taken together,these findings demonstrate the mechanism by which circRap1b inhibits neuronal apoptosis in acute ischemic stroke.
