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基于荧光光谱法研究酪蛋白与茶黄素类的相互作用 被引量:1
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作者 谢格格 张昊 +4 位作者 吴远杰 袁勇 银飞燕 吴浩人 石萌 《茶叶》 2024年第2期83-87,共5页
蛋白质和茶多酚是常见的互作体系,本项目旨在通过荧光光谱法研究酪蛋白和茶黄素类的相互作用,并通过不同温度下模型拟合确定其互作机制。结果表明,茶黄素类能够猝灭酪蛋白的自身荧光,猝灭类型为静态猝灭。当茶黄素类浓度为0.028 mg/mL时... 蛋白质和茶多酚是常见的互作体系,本项目旨在通过荧光光谱法研究酪蛋白和茶黄素类的相互作用,并通过不同温度下模型拟合确定其互作机制。结果表明,茶黄素类能够猝灭酪蛋白的自身荧光,猝灭类型为静态猝灭。当茶黄素类浓度为0.028 mg/mL时,在29℃、37℃和45℃温度下,酪蛋白的荧光强度分别下降了66.7%、55.6%、52.2%,且最大发射波长有轻微红移现象。在不同的温度下,酪蛋白均可自发地与茶黄素类结合,结合位点数约为1,结合方式主要以氢键和范德华力为主导。研究结果可为酪蛋白-茶黄素类复合物在功能食品中的应用提供参考。 展开更多
关键词 茶黄素类 酪蛋白 荧光猝灭 相互作用
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The effects of human activities on windbreak and sand fixation services in Inner Mongolia grasslands from 2000 to 2020
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作者 YAN Huimin xie gege +3 位作者 YAN Feng NIU Zhongen XUE Zhichao WANG Boyu 《Journal of Geographical Sciences》 SCIE CSCD 2024年第12期2421-2439,共19页
Windbreak and sand fixation services(SR)provided by grasslands are a joint result of climate change and human activities.Series of grassland protection measurements have been successively implemented on Inner Mongolia... Windbreak and sand fixation services(SR)provided by grasslands are a joint result of climate change and human activities.Series of grassland protection measurements have been successively implemented on Inner Mongolia grasslands since 2000,but their effects on SR remains unclear.Based on satellite-derived vegetation dynamics and the Revised Wind Erosion Equation(RWEQ)model,this paper developed a method for quantitatively separating the impact of human activities on SR and revealed the contribution of human activities to SR in the Inner Mongolia grasslands from 2000 to 2020.In 2020,the actual sand fixation(SRA)of Inner Mongolia grasslands was 12.50 t·ha^(-1),spatially characterized as lower in the eastern and western parts,which was dominated by the sparse vegetation coverage and the low potential wind erosion respectively,while higher in the central part,due to the grassland vulnerability.The human-driven sand fixation(SRH)of Inner Mongolia grasslands changed from–1.28 t·ha^(-1)to–0.14 t·ha^(-1)from 2000 to 2020,indicating human activities inhibited SR,but the inhibition was gradually weakened.In semidesert and meadow steppes,the SRHchanged from–3.00 t·ha^(-1)to 0.00 t·ha^(-1)and–0.16 t·ha^(-1)to 0.00 t·ha^(-1),respectively,which showed that the effect of human activities changed from inhibition to promotion.However,it should be noted that human activities still inhibited the SR in typical steppes.The results implicated that grassland ecological protection should pay much more attention to reasonable use of vulnerable typical steppes.Future grassland use requires quantitative evaluation on the effects of human activity for precise monitoring and sustainable management. 展开更多
关键词 windbreak and sand fixation services human activities RWEQ grassland ecosystem spatiotemporal change
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Impact of Human Activities and Climate Change on Grassland Productivity in Xilingol League
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作者 YAN Huimin xie gege +4 位作者 NIU Zhongen LIU Guihuan YANG Yanzhao XUE Zhichao WANG Boyu 《Journal of Resources and Ecology》 CSCD 2024年第5期1134-1146,共13页
Natural grasslands are increasingly subjected to the dual stresses of grazing pressures and climate change.However,the contribution of human activities,especially grassland ecology conservation projects,to grassland i... Natural grasslands are increasingly subjected to the dual stresses of grazing pressures and climate change.However,the contribution of human activities,especially grassland ecology conservation projects,to grassland improvement remains ambiguous.Utilizing MODIS satellite data in conjunction with the VPM model,the gross primary productivity(GPP)changes in the Xilingol grassland from 2000 to 2020 were assessed.Based on GPP data derived from remote sensing,this study quantitatively assessed the spatiotemporal dynamics of the impacts of climate change and human activities on the productivity of grassland in the Xilingol League.From 2000 to 2020,the grasslands exhibited a greening trend characterized by a significant annual GPP increment of 2.66 gC m^(-2) yr^(-1)(P<0.05).Climate change and human activities jointly contributed to this greening trend,with relative contribution rates of 55%and 45%,respectively.However,the relative contributions of climate change and human activities to the trend of GPP varied greatly in different regions.Climate change emerged as the principal driver in the central and eastern regions of Xilingol League with robust grass growth,accounting for more than 65%of the GPP enhancement.Conversely,human activities were the dominant factors in less verdant western regions and the agro-pastoral ecotone,representing more than 60%of the GPP change.Grassland productivity was sensitive to grassland ecological restoration measures,with significant changes in the trends of grassland productivity attributed to human activities in pivotal policy implementation years such as 2005 and 2011.Specifically,measures such as the control of wind/sand sources and returning grazing land to grassland from 2000 to 2005 gradually alleviated the pressure of human activities on grassland productivity,as they significantly improved vegetation growth in high-quality grasslands.Under the forage-livestock balance policy from 2005 to 2011,inadequate compensation for grassland ecological protection led to a significant reduction in GPP,as some herders increased their livestock holdings despite grazing restrictions,and this particularly affected the high-quality grasslands.The implementation of the Grassland Ecological Protection Subsidy and Reward Program from 2011 to 2020 generally promoted the recovery of productivity in eastern and western Xilingol League grasslands,but significant ecological pressure persisted.This study provides theoretical support for optimizing grassland ecosystem management and forming a virtuous cycle of grassland conservation in pastoral areas. 展开更多
关键词 ecological conservation projects gross primary productivity GRAZING Xilingol grassland
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