Background:Pyogenic granuloma(PG)is a benign vascular skin lesion that occurs in children.Although,sclerotherapy is a common treatment for patients with PG,all the previous studies have been case reports or series.At ...Background:Pyogenic granuloma(PG)is a benign vascular skin lesion that occurs in children.Although,sclerotherapy is a common treatment for patients with PG,all the previous studies have been case reports or series.At present,no reports have compared the efficacy of the two different sclerosing agents,polidocanol and bleomycin,in the treatment of PG.Therefore,we aimed to compare the cure rates and adverse reactions associated with these two agents in sclerotherapy for PG in children.Methods:This retrospective analysis included children<18 years of age with PG undergoing cutaneous treatment at our hospital between January 2016 and January 2022.Two sclerosing agents,polidocanol and bleomycin,were topically injected.The efficacy and incidence of adverse reactions were compared between the two groups.Results:A total of 117 children with PG were divided into the polidocanol(n=52)and bleomycin(n=65)groups.Lesions disappeared after one injection in 38 children,two in 11 children,and three in 3 children in the polidocanol group.A similar phenomenon was observed after one injection in 53 children,two injections in 8 children,and three injections in children in the bleomycin group.The single-injection cure rate was not significantly different between the two groups(P>0.05).The rate of adverse reactions was significantly different between the two groups(P<0.05).No severe complications occurred,and no recurrences were detected during the 6-12 months of postoperative follow-up period.Conclusion:This study showed that both polidocanol and bleomycin are safe and effective sclerosing agents for treatment of PG in children.The incidence of adverse reactions to polidocanol was lower than that to bleomycin.We recommend sclerotherapy with polidocanol as a first-line treatment for PG,as it is suitable for application in hospitals at various levels.展开更多
Chimeric antigen receptor T-cesll therapy(CAR–T)has achieved groundbreaking advancements in clinical application,ushering in a new era for innovative cancer treatment.However,the challenges associated with implementi...Chimeric antigen receptor T-cesll therapy(CAR–T)has achieved groundbreaking advancements in clinical application,ushering in a new era for innovative cancer treatment.However,the challenges associated with implementing this novel targeted cell therapy are increasingly significant.Particularly in the clinical management of solid tumors,obstacles such as the immunosuppressive effects of the tumor microenvironment,limited local tumor infiltration capability of CAR–T cells,heterogeneity of tumor targeting antigens,uncertainties surrounding CAR–T quality,control,and clinical adverse reactions have contributed to increased drug resistance and decreased compliance in tumor therapy.These factors have significantly impeded the widespread adoption and utilization of this therapeutic approach.In this paper,we comprehensively analyze recent preclinical and clinical reports on CAR–T therapy while summarizing crucial factors influencing its efficacy.Furthermore,we aim to identify existing solution strategies and explore their current research status.Through this review article,our objective is to broaden perspectives for further exploration into CAR–T therapy strategies and their clinical applications.展开更多
To measure miss distance for antiaircraft projectile,a radial velocity identification and positioning method with a single radar is proposed. By analyzing the spatial resolution of multi-frequency ranging radar,the di...To measure miss distance for antiaircraft projectile,a radial velocity identification and positioning method with a single radar is proposed. By analyzing the spatial resolution of multi-frequency ranging radar,the discrimination and testing model of this radar for multi-targets（ projectile and target） is established to analyze the systematic error of antiaircraft miss distance. Then through the aerial target flight test and contrast test with optical test equipment,the validity of the measurement method is verified. This newmethod has the potential to be used in the measurement of antiaircraft projectile miss distance.展开更多
The UFMylation modification is a novel ubiquitin-like conjugation system,consisting of UBA5(E1),UFC1(E2),UFL1(E3),and the conjugating molecule UFM1.Deficiency in this modification leads to embryonic lethality in mice ...The UFMylation modification is a novel ubiquitin-like conjugation system,consisting of UBA5(E1),UFC1(E2),UFL1(E3),and the conjugating molecule UFM1.Deficiency in this modification leads to embryonic lethality in mice and diseases in humans.However,the function of UFL1 is poorly characterized.Studies on Ufl1 conditional knockout mice have demonstrated that the deletion of Ufl1 in cardiomyocytes and in intestinal epithelial cells causes heart failure and increases susceptibility to experimentally induced colitis,respectively,suggesting an essential role of UFL1 in the maintenance of the homeostasis in these organs.Yet,its physiological function in other tissues and organs remains completely unknown.In this study,we generate the nephron tubules specific Ufl1 knockout mice and find that the absence of Ufl1 in renal tubular results in kidney atrophy and interstitial fibrosis.In addition,Ufl1 deficiency causes the activation of unfolded protein response and cell apoptosis,which may be responsible for the kidney atrophy and interstitial fibrosis.Collectively,our results have demonstrated the crucial role of UFL1 in regulating kidney function and maintenance of endoplasmic reticulum homeostasis,providing another layer of understanding kidney atrophy.展开更多
Previous studies have demonstrated that nerve cells differentiated from adipose-derived stro-mal cells after chemical induction have reduced viability;however, the underlying mechanisms remained unclear. In this study...Previous studies have demonstrated that nerve cells differentiated from adipose-derived stro-mal cells after chemical induction have reduced viability;however, the underlying mechanisms remained unclear. In this study, we induced the differentiation of adult adipose-derived stromal cells into astrocytes using chemical induction. 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetra-zolium bromide assay and flow cytometry showed that, with increasing induction time, the apoptotic rate gradually increased, and the number of living cells gradually decreased. Im-munohistochemical staining demonstrated that the number of glial fibrillary acidic protein-, caspase-3- and caspase-9-positive cells gradually increased with increasing induction time. Transmission electron microscopy revealed typical signs of apoptosis after differentiation. Taken together, our results indicate that caspase-dependent apoptosis is an obstacle to the differentia-tion of adipose-derived stromal cells into astrocytes. Inhibiting apoptosis may be an important strategy for increasing the efifciency of induction.展开更多
BACKGROUND： The detection of motor evoked potential is utilized to explore neuromuscular finger coordination. The influence of transcranial magnetic stimulation on finger force has been investigated mainly on a singl...BACKGROUND： The detection of motor evoked potential is utilized to explore neuromuscular finger coordination. The influence of transcranial magnetic stimulation on finger force has been investigated mainly on a single finger, and only time-dependent increased target finger force has been detected in the finger force task. OBJECTIVE： To explore the neural mechanism of finger force coordination in the motor cortex by observing the influence of various finger coordination patterns and patterns of transcranial magnetic stimulation （TMS）-induced finger force changes. DESIGN, TIME AND SETTING： Neurophysiological and behavioral study was performed at the Biomedical Engineering Laboratory of Chongqing University from April to June 2008. PARTICIPANTS： A total of 10 healthy, university students, comprising 5 males and 5 females, aged 21-23 years, voluntarily participated in this study. All participants were right-handed, with normal or corrected vision. Individuals with upper limb complaints or other musculoskeletal disorders were excluded. METHODS： A target force-tracking task was conducted on the index finger, the index and middle fingers, and four fingers （index, middle, ring, and little）, respectively. Target force trace in a single trial consisted of a 6-second ramp phase, a 20-second constant phase, and a 6-second drop phase. During experimentation, an unpredictable single-pulse TMS （120% motor threshold） was applied to the primary motor cortex （M1） in each phase. MAIN OUTCOME MEASURES： Changes in peak force induced by TMS were obtained for each finger pattern during each force-tracking phase. Differences in force changes were tested between different finger pattems with regard to ramp, constant, and drop phases of target force. RESULTS： Under ramp, constant, and drop phases of target force, the increase in magnetic stimulation-induced finger forces changes positively correlated with the number of fingers involved in the force tracking task. The magnetic stimulation-induced force changes from the index finger were less than the combination of the index and middle fingers or all four fingers under the corresponding target force, and the force changes from the combination of the index and middle fingers were less than all four fingers, Le., index finger 〈 index and middle fingers 〈 four fingers. CONCLUSION： Different neuromuscular mechanisms could be involved in finger force production for different finger combination patterns. Results from the present study suggested that independent motor neurons regulated individual finger force production.展开更多
Bone marrow mesenchymal stem cell transplantation is an effective treatment for neonatal hy- poxic-ischemic brain damage. However, the in vivo transplantation effects are poor and their survival, colonization and diff...Bone marrow mesenchymal stem cell transplantation is an effective treatment for neonatal hy- poxic-ischemic brain damage. However, the in vivo transplantation effects are poor and their survival, colonization and differentiation efficiencies are relatively low. Red or near-infrared light from 600-1,000 nm promotes cellular migration and prevents apoptosis. Thus, we hypothesized that the combination of red light with bone marrow mesenchymal stem cell transplantation would be effective for the treatment of hypoxic-ischemic brain damage. In this study, the migra- tion and colonization of cultured bone marrow mesenchymal stem cells on primary neurons after oxygen-glucose deprivation were detected using Transwell assay. The results showed that, after a 40-hour irradiation under red light-emitting diodes at 660 nm and 60 mW/cmz, an increasing number of green fluorescence-labeled bone marrow mesenchymal stem cells migrated towards hypoxic-ischemic damaged primary neurons. Meanwhile, neonatal rats with hypoxic-ischemic brain damage were given an intraperitoneal injection of 1 x 106 bone marrow mesenchymal stem cells, followed by irradiation under red light-emitting diodes at 660 nm and 60 mW/cm2 for 7 successive days. Shuttle box test results showed that, after phototherapy and bone marrow mesenchymal stem cell transplantation, the active avoidance response rate of hypoxic-ischemic brain damage rats was significantly increased, which was higher than that after bone marrow mesenchymal stem cell transplantation alone. Experimental findings indicate that 660 nm red light emitting diode irradiation promotes cells, thereby enhancing the contribution ic-ischemic brain damage. the migration of bone marrow mesenchymal stem of cell transplantation in the treatment of hypox-展开更多
A better understanding of the neural mechanisms of finger-force regulation can help to explain the relationship between the central nervous system and nerve-muscle force, as well as assist in motor functional rehabili...A better understanding of the neural mechanisms of finger-force regulation can help to explain the relationship between the central nervous system and nerve-muscle force, as well as assist in motor functional rehabilitation and the development robot hand designs. In the present study, 11 healthy volunteers performed a different target force-tracking task, which involved the index finger alone, index and middle finger together, and the combination of four fingers （i.e., index, middle, ring, and little）. The target force trace corresponded to 3 levels of 20% maximal voluntary changes （MVC）, 30% MVC, and 40% MVC in 20 seconds. In the test, an unexpected single 120% motor threshold transcranial magnetic stimulation was applied to the primary motor cortex （M1） during force tracking. Results revealed that peak force changes increased with increasing background force and the number of involved task fingers. These results demonstrate that M1 neural activities correlate with finger-force production, and M1 plays a role in finger-force control. Moreover, different neuronal networks were required for different finger patterns; a complicated task required multi-finger combinations and a complicated neuronal network comprised a large number of neurons.展开更多
The problem of measuring exterior ballistic feature points is always difficult to solve and it is essentiale on exterior ballistic measurement.By analysis of radar reflection characteristics and non-stationary echo si...The problem of measuring exterior ballistic feature points is always difficult to solve and it is essentiale on exterior ballistic measurement.By analysis of radar reflection characteristics and non-stationary echo signals of exterior ballistic feature points,the echo data of exterior ballistic feature points is measured by using the continuous wave radar.The parameters of feature points are extracted by the empirical mode decomposition method(EMD)of Hilbert-Huang transform(HHT)spectrum analysis technique.The radar echo signal model and EMD extraction model are established to analyze the exterior ballistic mutation point detection and EMD extraction method of aliasing echo signal.Typical feature point parameters of exterior ballistic in rocket flight tests are carried out and the effectiveness of the method is verified.A new method of measuring the parameters of exterior ballistic feature point is therefore presented.展开更多
Renal amyloidosis secondary to anti-neutrophil cytoplasmic antibody(ANCA)-associated vasculitis is extremely rare.Here,we reported a 77-year-old woman with ANCA-associated vasculitis.Renal biopsy with Masson trichrome...Renal amyloidosis secondary to anti-neutrophil cytoplasmic antibody(ANCA)-associated vasculitis is extremely rare.Here,we reported a 77-year-old woman with ANCA-associated vasculitis.Renal biopsy with Masson trichrome staining showed pauci-immune crescentic glomerulonephritis,and electron microscopy showed amyloid deposition in the mesangial area.Immunofluorescence revealed kappa light chain and lambda light chain negative.Bone marrow biopsy revealed no clonal plasma cell.Finally,she was diagnosed as ANCA-associated vasculitis with secondary renal amyloid A amyloidosis.展开更多
[Objectives]To investigate the inhibitory effect and possible mechanism of essential oil from Valerianae Jatamansi Rhizoma et Radix on microglia activation induced by lipopolysaccharide(LPS).[Methods]The LPS-induced m...[Objectives]To investigate the inhibitory effect and possible mechanism of essential oil from Valerianae Jatamansi Rhizoma et Radix on microglia activation induced by lipopolysaccharide(LPS).[Methods]The LPS-induced microglia activation model was established and treated with different doses of essential oil from Valerianae Jatamansi Rhizoma et Radix.MTT assay was used to detect cell viability,ELISA to detect IL-6 secretion,RT-PCR to detect mRNA expression levels of IL-6,IL-1β,NF-κB,and IκBα,Western blotting to detect the protein expression of IL-6,IL-1β,NF-κB,IκBα,and their phosphorylated products.[Results]Compared with the normal control group,the model group showed increased IL-6 content(P<0.01),upregulated mRNA and protein levels of IL-6,IL-1β,and NF-κB(P<0.01),and elevated ratio of P-IκBα/IκBα(P<0.05).Compared with the model group,4 and 2μg/L essential oil from Valerianae Jatamansi Rhizoma et Radix reduced the content of IL-6(P<0.05),while 4,2,and 1μg/L essential oil from Valerianae Jatamansi Rhizoma et Radix down-regulated the mRNA and protein levels of IL-6,IL-1β,and NF-κB to varying degrees(P<0.05 or P<0.01),up-regulate the mRNA expression of IκBα(P<0.05 or P<0.01),and decreased the ratio of P-IκBα/IκBα(P<0.05 or P<0.01).[Conclusions]Essential oil from Valerianae Jatamansi Rhizoma et Radix can inhibit LPS-induced microglia activation,and its mechanism may be related to the inhibition of the NF-κB/IκBαsignaling pathway.展开更多
Background:Currently,traditional methods of treating acute gastric ulcer(AGU)have many drawbacks,necessitating an alternative therapy with fewer adverse effects.Atractylodis Macrocephalae Rhizoma(BZ)is known for stren...Background:Currently,traditional methods of treating acute gastric ulcer(AGU)have many drawbacks,necessitating an alternative therapy with fewer adverse effects.Atractylodis Macrocephalae Rhizoma(BZ)is known for strengthening the spleen and harmonizing the stomach.BZ processed with Aurantii Fructus Immaturus juice(ZSZBZ),a classic decoction since the Han Dynasty,can enhance the efficacy of BZ.However,the key active components and targets of action of ZSZBZ remain undiscovered.Aimof the study:This study aimed to investigate the bioactive chemical constituents of ZSZBZ against AGU and their possible mechanisms of action,elucidating the scientific content of ZSZBZ processing.Materials and methods:Initially,we examined rat stomach histopathology and conducted ELISA for oxidative stress and inflammation.Subsequently,we investigated underlying mechanisms using metabolomics.Further analysis of potent components and key targets in ZSZBZ was conducted through liquid chromatography-mass spectrometry analysis combined with network pharmacology.Finally,key targets were analyzed by Western blot.Results:ZSZBZ improved gastric histopathology,reversing high alcohol-induced oxidative stress(SOD,CAT)and inflammatory level(TNF-α,IL-6)disorders.This is associated with ZSZBZ’s regulation of amino acid metabolism,energy metabolism,and inflammatory response-related metabolic pathways,along with key targets PTGS2,MAPK1,and KDR.The significant increase in potency of ZSZBZ may be attributed to elevated levels of naringenin,hesperidin,hesperidin,and rhamnoceroside after concoction.Conclusions:Combining metabolomics and network pharmacology,this study elucidated that ZSZBZ enhanced gastroprotection by modulating amino acid metabolism,antioxidant,and inflammation-related targets and pathways,providing insights into the bioactive compounds and potential mechanisms of herbal concoctions.展开更多
What is already known about this topic?S.1,4,[5],12:i:-and S.Rissen are emerging serotypes of Salmonella that require close monitoring for antimicrobial resistance and containment of their spread.What is added by this...What is already known about this topic?S.1,4,[5],12:i:-and S.Rissen are emerging serotypes of Salmonella that require close monitoring for antimicrobial resistance and containment of their spread.What is added by this report?The study aimed to identify antimicrobial resistance genes(ARGs)in S.1,4,[5],12:i:-and S.Rissen strains isolated from environmental sewage in Guangzhou City,Guangdong Province,China.A phylogenetic tree was constructed using single nucleotide polymorphism data to assess genetic relatedness among strains,offering insights for Salmonella infection outbreak investigations in the future.What are the implications for public health practice?It is crucial to implement strategies,such as integrating different networks,to control the spread of drugresistant Salmonella.Novel technologies must be utilized to disinfect sewage and eliminate ARGs.Ensuring food safety and proper sewage disinfection are essential to curb the dissemination of Salmonella.展开更多
Background:Recent studies have found that thyroid function may be associated with the occurrence and development of advanced liver fibrosis in patients with metabolic dysfunction-associated fatty liver disease(MAFLD)....Background:Recent studies have found that thyroid function may be associated with the occurrence and development of advanced liver fibrosis in patients with metabolic dysfunction-associated fatty liver disease(MAFLD).However,the majority of such research has consisted of cross-sectional studies.This retrospective cohort study aimed to investigate the effect of low-normal thyroid function on advanced liver fibrosis in MAFLD patients over a 5-year period.Methods:This retrospective cohort study enrolled 825 outpatients and inpatients with MAFLD who attended the Third Affiliated Hospital of Sun Yat-sen University(Guangzhou,China)between January 2011 and December 2018.Based on plasma thyroid hormone and thyroid-stimulating hormone levels,these patients were divided into two groups,namely a low-normal thyroid function group and a strict-normal thyroid function group.The fibrosis-4 score was used to assess advanced liver fibrosis.A chi-square test was conducted to compare the occurrence of advanced fibrosis between the groups.Results:Among the 825 MAFLD patients,117 and 708 were defined as having low-normal thyroid function and strict-normal thyroid function,respectively.Follow-up data were available for 767 patients(93.0%)during a 5-year period.Eight(7.5%)MAFLD patients with low-normal thyroid function and 26(3.9%)with strict-normal thyroid function developed advanced liver fibrosis and the cumulative incidence was not significantly different(P=0.163).Stratification analysis showed that the lean MAFLD patients(body mass index≤23kg/m^(2))with low-normal thyroid function had a higher risk of advanced liver fibrosis than the lean MAFLD patients with strict-normal thyroid function(P<0.05).Conclusion:Low-normal thyroid function is associated with advanced liver fibrosis among lean MAFLD patients.展开更多
文摘Background:Pyogenic granuloma(PG)is a benign vascular skin lesion that occurs in children.Although,sclerotherapy is a common treatment for patients with PG,all the previous studies have been case reports or series.At present,no reports have compared the efficacy of the two different sclerosing agents,polidocanol and bleomycin,in the treatment of PG.Therefore,we aimed to compare the cure rates and adverse reactions associated with these two agents in sclerotherapy for PG in children.Methods:This retrospective analysis included children<18 years of age with PG undergoing cutaneous treatment at our hospital between January 2016 and January 2022.Two sclerosing agents,polidocanol and bleomycin,were topically injected.The efficacy and incidence of adverse reactions were compared between the two groups.Results:A total of 117 children with PG were divided into the polidocanol(n=52)and bleomycin(n=65)groups.Lesions disappeared after one injection in 38 children,two in 11 children,and three in 3 children in the polidocanol group.A similar phenomenon was observed after one injection in 53 children,two injections in 8 children,and three injections in children in the bleomycin group.The single-injection cure rate was not significantly different between the two groups(P>0.05).The rate of adverse reactions was significantly different between the two groups(P<0.05).No severe complications occurred,and no recurrences were detected during the 6-12 months of postoperative follow-up period.Conclusion:This study showed that both polidocanol and bleomycin are safe and effective sclerosing agents for treatment of PG in children.The incidence of adverse reactions to polidocanol was lower than that to bleomycin.We recommend sclerotherapy with polidocanol as a first-line treatment for PG,as it is suitable for application in hospitals at various levels.
基金funded by 2023 Sichuan Scientific and Technological Achievements Transformation Project.Project Number:2023JDZH0024.
文摘Chimeric antigen receptor T-cesll therapy(CAR–T)has achieved groundbreaking advancements in clinical application,ushering in a new era for innovative cancer treatment.However,the challenges associated with implementing this novel targeted cell therapy are increasingly significant.Particularly in the clinical management of solid tumors,obstacles such as the immunosuppressive effects of the tumor microenvironment,limited local tumor infiltration capability of CAR–T cells,heterogeneity of tumor targeting antigens,uncertainties surrounding CAR–T quality,control,and clinical adverse reactions have contributed to increased drug resistance and decreased compliance in tumor therapy.These factors have significantly impeded the widespread adoption and utilization of this therapeutic approach.In this paper,we comprehensively analyze recent preclinical and clinical reports on CAR–T therapy while summarizing crucial factors influencing its efficacy.Furthermore,we aim to identify existing solution strategies and explore their current research status.Through this review article,our objective is to broaden perspectives for further exploration into CAR–T therapy strategies and their clinical applications.
基金Supported by the National Natural Science Foundation of China(61174219,51677192)
文摘To measure miss distance for antiaircraft projectile,a radial velocity identification and positioning method with a single radar is proposed. By analyzing the spatial resolution of multi-frequency ranging radar,the discrimination and testing model of this radar for multi-targets（ projectile and target） is established to analyze the systematic error of antiaircraft miss distance. Then through the aerial target flight test and contrast test with optical test equipment,the validity of the measurement method is verified. This newmethod has the potential to be used in the measurement of antiaircraft projectile miss distance.
基金supported by grants from the National Natural Science Foundation of China(31871416,31730020)the Natural Science Foundation of ZhejiangProvince of China(LY18C070001)the Hangzhou Science and Technology Bureau(20182014B01,20180533B27)。
文摘The UFMylation modification is a novel ubiquitin-like conjugation system,consisting of UBA5(E1),UFC1(E2),UFL1(E3),and the conjugating molecule UFM1.Deficiency in this modification leads to embryonic lethality in mice and diseases in humans.However,the function of UFL1 is poorly characterized.Studies on Ufl1 conditional knockout mice have demonstrated that the deletion of Ufl1 in cardiomyocytes and in intestinal epithelial cells causes heart failure and increases susceptibility to experimentally induced colitis,respectively,suggesting an essential role of UFL1 in the maintenance of the homeostasis in these organs.Yet,its physiological function in other tissues and organs remains completely unknown.In this study,we generate the nephron tubules specific Ufl1 knockout mice and find that the absence of Ufl1 in renal tubular results in kidney atrophy and interstitial fibrosis.In addition,Ufl1 deficiency causes the activation of unfolded protein response and cell apoptosis,which may be responsible for the kidney atrophy and interstitial fibrosis.Collectively,our results have demonstrated the crucial role of UFL1 in regulating kidney function and maintenance of endoplasmic reticulum homeostasis,providing another layer of understanding kidney atrophy.
文摘Previous studies have demonstrated that nerve cells differentiated from adipose-derived stro-mal cells after chemical induction have reduced viability;however, the underlying mechanisms remained unclear. In this study, we induced the differentiation of adult adipose-derived stromal cells into astrocytes using chemical induction. 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetra-zolium bromide assay and flow cytometry showed that, with increasing induction time, the apoptotic rate gradually increased, and the number of living cells gradually decreased. Im-munohistochemical staining demonstrated that the number of glial fibrillary acidic protein-, caspase-3- and caspase-9-positive cells gradually increased with increasing induction time. Transmission electron microscopy revealed typical signs of apoptosis after differentiation. Taken together, our results indicate that caspase-dependent apoptosis is an obstacle to the differentia-tion of adipose-derived stromal cells into astrocytes. Inhibiting apoptosis may be an important strategy for increasing the efifciency of induction.
基金the National Natural Science Foundation of China, No. 3077054630970758+1 种基金Chongqing Natural Science Foundation, No. 2006BB2043 2007BB5148
文摘BACKGROUND： The detection of motor evoked potential is utilized to explore neuromuscular finger coordination. The influence of transcranial magnetic stimulation on finger force has been investigated mainly on a single finger, and only time-dependent increased target finger force has been detected in the finger force task. OBJECTIVE： To explore the neural mechanism of finger force coordination in the motor cortex by observing the influence of various finger coordination patterns and patterns of transcranial magnetic stimulation （TMS）-induced finger force changes. DESIGN, TIME AND SETTING： Neurophysiological and behavioral study was performed at the Biomedical Engineering Laboratory of Chongqing University from April to June 2008. PARTICIPANTS： A total of 10 healthy, university students, comprising 5 males and 5 females, aged 21-23 years, voluntarily participated in this study. All participants were right-handed, with normal or corrected vision. Individuals with upper limb complaints or other musculoskeletal disorders were excluded. METHODS： A target force-tracking task was conducted on the index finger, the index and middle fingers, and four fingers （index, middle, ring, and little）, respectively. Target force trace in a single trial consisted of a 6-second ramp phase, a 20-second constant phase, and a 6-second drop phase. During experimentation, an unpredictable single-pulse TMS （120% motor threshold） was applied to the primary motor cortex （M1） in each phase. MAIN OUTCOME MEASURES： Changes in peak force induced by TMS were obtained for each finger pattern during each force-tracking phase. Differences in force changes were tested between different finger pattems with regard to ramp, constant, and drop phases of target force. RESULTS： Under ramp, constant, and drop phases of target force, the increase in magnetic stimulation-induced finger forces changes positively correlated with the number of fingers involved in the force tracking task. The magnetic stimulation-induced force changes from the index finger were less than the combination of the index and middle fingers or all four fingers under the corresponding target force, and the force changes from the combination of the index and middle fingers were less than all four fingers, Le., index finger 〈 index and middle fingers 〈 four fingers. CONCLUSION： Different neuromuscular mechanisms could be involved in finger force production for different finger combination patterns. Results from the present study suggested that independent motor neurons regulated individual finger force production.
基金the National Natural Science Foundation of China,No.30970758,31271060the National Science and Technology Support Program of China,No.2011BAI14B04,2012BAI16B02the Natural Science Foundation of Chongqing in China,No.cst-c2012jjA10103
文摘Bone marrow mesenchymal stem cell transplantation is an effective treatment for neonatal hy- poxic-ischemic brain damage. However, the in vivo transplantation effects are poor and their survival, colonization and differentiation efficiencies are relatively low. Red or near-infrared light from 600-1,000 nm promotes cellular migration and prevents apoptosis. Thus, we hypothesized that the combination of red light with bone marrow mesenchymal stem cell transplantation would be effective for the treatment of hypoxic-ischemic brain damage. In this study, the migra- tion and colonization of cultured bone marrow mesenchymal stem cells on primary neurons after oxygen-glucose deprivation were detected using Transwell assay. The results showed that, after a 40-hour irradiation under red light-emitting diodes at 660 nm and 60 mW/cmz, an increasing number of green fluorescence-labeled bone marrow mesenchymal stem cells migrated towards hypoxic-ischemic damaged primary neurons. Meanwhile, neonatal rats with hypoxic-ischemic brain damage were given an intraperitoneal injection of 1 x 106 bone marrow mesenchymal stem cells, followed by irradiation under red light-emitting diodes at 660 nm and 60 mW/cm2 for 7 successive days. Shuttle box test results showed that, after phototherapy and bone marrow mesenchymal stem cell transplantation, the active avoidance response rate of hypoxic-ischemic brain damage rats was significantly increased, which was higher than that after bone marrow mesenchymal stem cell transplantation alone. Experimental findings indicate that 660 nm red light emitting diode irradiation promotes cells, thereby enhancing the contribution ic-ischemic brain damage. the migration of bone marrow mesenchymal stem of cell transplantation in the treatment of hypox-
基金the Fundamental Research Funds for the Central Universities,No.CDJZR11230002
文摘A better understanding of the neural mechanisms of finger-force regulation can help to explain the relationship between the central nervous system and nerve-muscle force, as well as assist in motor functional rehabilitation and the development robot hand designs. In the present study, 11 healthy volunteers performed a different target force-tracking task, which involved the index finger alone, index and middle finger together, and the combination of four fingers （i.e., index, middle, ring, and little）. The target force trace corresponded to 3 levels of 20% maximal voluntary changes （MVC）, 30% MVC, and 40% MVC in 20 seconds. In the test, an unexpected single 120% motor threshold transcranial magnetic stimulation was applied to the primary motor cortex （M1） during force tracking. Results revealed that peak force changes increased with increasing background force and the number of involved task fingers. These results demonstrate that M1 neural activities correlate with finger-force production, and M1 plays a role in finger-force control. Moreover, different neuronal networks were required for different finger patterns; a complicated task required multi-finger combinations and a complicated neuronal network comprised a large number of neurons.
基金Supported by the National Natural Science Foundation of China(61174219,51677192)
文摘The problem of measuring exterior ballistic feature points is always difficult to solve and it is essentiale on exterior ballistic measurement.By analysis of radar reflection characteristics and non-stationary echo signals of exterior ballistic feature points,the echo data of exterior ballistic feature points is measured by using the continuous wave radar.The parameters of feature points are extracted by the empirical mode decomposition method(EMD)of Hilbert-Huang transform(HHT)spectrum analysis technique.The radar echo signal model and EMD extraction model are established to analyze the exterior ballistic mutation point detection and EMD extraction method of aliasing echo signal.Typical feature point parameters of exterior ballistic in rocket flight tests are carried out and the effectiveness of the method is verified.A new method of measuring the parameters of exterior ballistic feature point is therefore presented.
文摘Renal amyloidosis secondary to anti-neutrophil cytoplasmic antibody(ANCA)-associated vasculitis is extremely rare.Here,we reported a 77-year-old woman with ANCA-associated vasculitis.Renal biopsy with Masson trichrome staining showed pauci-immune crescentic glomerulonephritis,and electron microscopy showed amyloid deposition in the mesangial area.Immunofluorescence revealed kappa light chain and lambda light chain negative.Bone marrow biopsy revealed no clonal plasma cell.Finally,she was diagnosed as ANCA-associated vasculitis with secondary renal amyloid A amyloidosis.
基金Supported by Karst Center Project of National Natural Science Foundation of China(U1812403-4-4)High-level Innovative Talents Project in Guizhou Province of Guizhou Provincial Department of Science and Technology[QianKeHeRenCai(2015)4029]+2 种基金Science and Technology Innovation Team for Activity Research of Characteristic Natural Medicine Resources in Guizhou Province[QianKeHeRenCaiTuanDui(2015)4025]Major Project of National Social Science Fund(16ZDA238)Pharmaceutical International Science and Technology Cooperation Base of Guizhou Medical University[QianKeHePingTaiRenCai(2017)5802].
文摘[Objectives]To investigate the inhibitory effect and possible mechanism of essential oil from Valerianae Jatamansi Rhizoma et Radix on microglia activation induced by lipopolysaccharide(LPS).[Methods]The LPS-induced microglia activation model was established and treated with different doses of essential oil from Valerianae Jatamansi Rhizoma et Radix.MTT assay was used to detect cell viability,ELISA to detect IL-6 secretion,RT-PCR to detect mRNA expression levels of IL-6,IL-1β,NF-κB,and IκBα,Western blotting to detect the protein expression of IL-6,IL-1β,NF-κB,IκBα,and their phosphorylated products.[Results]Compared with the normal control group,the model group showed increased IL-6 content(P<0.01),upregulated mRNA and protein levels of IL-6,IL-1β,and NF-κB(P<0.01),and elevated ratio of P-IκBα/IκBα(P<0.05).Compared with the model group,4 and 2μg/L essential oil from Valerianae Jatamansi Rhizoma et Radix reduced the content of IL-6(P<0.05),while 4,2,and 1μg/L essential oil from Valerianae Jatamansi Rhizoma et Radix down-regulated the mRNA and protein levels of IL-6,IL-1β,and NF-κB to varying degrees(P<0.05 or P<0.01),up-regulate the mRNA expression of IκBα(P<0.05 or P<0.01),and decreased the ratio of P-IκBα/IκBα(P<0.05 or P<0.01).[Conclusions]Essential oil from Valerianae Jatamansi Rhizoma et Radix can inhibit LPS-induced microglia activation,and its mechanism may be related to the inhibition of the NF-κB/IκBαsignaling pathway.
基金funded by the Jiangxi Provincial Natural Science Foundation Youth Project(20212BAB216011)the Second National Famous ChineseMedicine Practitioner(Gong Qianfeng)Inheritance Workshop of the State Administration of Traditional ChineseMedicine(State Chinese Medicine Office Human Education Letter[2022]no.245)+2 种基金2023 National Chinese Medicine Characteristic Technology Inheritance Talent Training Program(State Chinese Medicine Human Education Letter[2023]no.96)Jiangxi Province,the young and medium-aged backbone talents of traditional Chinese medicine(the fourth batch)(Gan TCM Science and Education[2022]no.7)the seventh batch of the national old Chinese medicine experts in academic experience successors(State Chinesemedicine human education letter[2022]no.76).
文摘Background:Currently,traditional methods of treating acute gastric ulcer(AGU)have many drawbacks,necessitating an alternative therapy with fewer adverse effects.Atractylodis Macrocephalae Rhizoma(BZ)is known for strengthening the spleen and harmonizing the stomach.BZ processed with Aurantii Fructus Immaturus juice(ZSZBZ),a classic decoction since the Han Dynasty,can enhance the efficacy of BZ.However,the key active components and targets of action of ZSZBZ remain undiscovered.Aimof the study:This study aimed to investigate the bioactive chemical constituents of ZSZBZ against AGU and their possible mechanisms of action,elucidating the scientific content of ZSZBZ processing.Materials and methods:Initially,we examined rat stomach histopathology and conducted ELISA for oxidative stress and inflammation.Subsequently,we investigated underlying mechanisms using metabolomics.Further analysis of potent components and key targets in ZSZBZ was conducted through liquid chromatography-mass spectrometry analysis combined with network pharmacology.Finally,key targets were analyzed by Western blot.Results:ZSZBZ improved gastric histopathology,reversing high alcohol-induced oxidative stress(SOD,CAT)and inflammatory level(TNF-α,IL-6)disorders.This is associated with ZSZBZ’s regulation of amino acid metabolism,energy metabolism,and inflammatory response-related metabolic pathways,along with key targets PTGS2,MAPK1,and KDR.The significant increase in potency of ZSZBZ may be attributed to elevated levels of naringenin,hesperidin,hesperidin,and rhamnoceroside after concoction.Conclusions:Combining metabolomics and network pharmacology,this study elucidated that ZSZBZ enhanced gastroprotection by modulating amino acid metabolism,antioxidant,and inflammation-related targets and pathways,providing insights into the bioactive compounds and potential mechanisms of herbal concoctions.
基金Supported by Guangdong Basic and Applied Basic Research Foundation(2021A1515012539)Science and Technology Program of Guangzhou+8 种基金China(202102080295)Guangzhou Key Medical Discipline(2021-2023-11)2023 Huadu District Medical and Health General Scientific Research Special Project of Guangzhou Huadu District Bureau of ScienceTechnologyIndustryCommerceand Information Technology(23-HDWS-079)Key R&D Plan of Guangzhou Science and Technology Project(202206080003)Guangzhou Science and Technology Plan Project(2023A03J0938).
文摘What is already known about this topic?S.1,4,[5],12:i:-and S.Rissen are emerging serotypes of Salmonella that require close monitoring for antimicrobial resistance and containment of their spread.What is added by this report?The study aimed to identify antimicrobial resistance genes(ARGs)in S.1,4,[5],12:i:-and S.Rissen strains isolated from environmental sewage in Guangzhou City,Guangdong Province,China.A phylogenetic tree was constructed using single nucleotide polymorphism data to assess genetic relatedness among strains,offering insights for Salmonella infection outbreak investigations in the future.What are the implications for public health practice?It is crucial to implement strategies,such as integrating different networks,to control the spread of drugresistant Salmonella.Novel technologies must be utilized to disinfect sewage and eliminate ARGs.Ensuring food safety and proper sewage disinfection are essential to curb the dissemination of Salmonella.
基金supported by Guangzhou Science and Technology Bureau.Guangzhou Science and Technology Bureau Project:Basic and clinical research of traditional Chinese medicine berberine in the treatment of non-alcoholic fatty liver disease by promoting intestinal GLP-1 secretion[No.201903010099].
文摘Background:Recent studies have found that thyroid function may be associated with the occurrence and development of advanced liver fibrosis in patients with metabolic dysfunction-associated fatty liver disease(MAFLD).However,the majority of such research has consisted of cross-sectional studies.This retrospective cohort study aimed to investigate the effect of low-normal thyroid function on advanced liver fibrosis in MAFLD patients over a 5-year period.Methods:This retrospective cohort study enrolled 825 outpatients and inpatients with MAFLD who attended the Third Affiliated Hospital of Sun Yat-sen University(Guangzhou,China)between January 2011 and December 2018.Based on plasma thyroid hormone and thyroid-stimulating hormone levels,these patients were divided into two groups,namely a low-normal thyroid function group and a strict-normal thyroid function group.The fibrosis-4 score was used to assess advanced liver fibrosis.A chi-square test was conducted to compare the occurrence of advanced fibrosis between the groups.Results:Among the 825 MAFLD patients,117 and 708 were defined as having low-normal thyroid function and strict-normal thyroid function,respectively.Follow-up data were available for 767 patients(93.0%)during a 5-year period.Eight(7.5%)MAFLD patients with low-normal thyroid function and 26(3.9%)with strict-normal thyroid function developed advanced liver fibrosis and the cumulative incidence was not significantly different(P=0.163).Stratification analysis showed that the lean MAFLD patients(body mass index≤23kg/m^(2))with low-normal thyroid function had a higher risk of advanced liver fibrosis than the lean MAFLD patients with strict-normal thyroid function(P<0.05).Conclusion:Low-normal thyroid function is associated with advanced liver fibrosis among lean MAFLD patients.
