To examine the possible correlation of aberrant Wnt signaling and pathological changes in Alzheimer’s disease, we established a rat model of Alzheimer’s disease and measured axin and β-catenin expression in the hip...To examine the possible correlation of aberrant Wnt signaling and pathological changes in Alzheimer’s disease, we established a rat model of Alzheimer’s disease and measured axin and β-catenin expression in the hippocampus. Rats were pretreated with moxibustion or electroacu-puncture, or both, at Baihui（GV20） and Shenshu（BL23）. Axin expression was lower, β-catenin expression was greater, and neuronal cytoplasmic edema was visibly prevented in the rats that had received the pretreatments. Our results suggest that the mechanism underlying the neuro-protective effect of acupuncture and moxibustion in Alzheimer’s disease is associated with axin and β-catenin expression in the Wnt signal transduction pathway.展开更多
Background:Nuclear receptor binding SET domain protein-3(NSD3)is a histone lysine methyltransferase and a crucial regulator of carcinogenesis in several cancers.We aimed to investigate the prognostic value and potenti...Background:Nuclear receptor binding SET domain protein-3(NSD3)is a histone lysine methyltransferase and a crucial regulator of carcinogenesis in several cancers.We aimed to investigate the prognostic value and potential function of NSD3 in 33 types of human cancer.Methods:The data were obtained from The Cancer Genome Atlas.Kaplan-Meier analysis,CIBERSORT,gene set enrichment analysis,and gene set variation analysis were performed.The expression of NSD3 was measured using quantitative real-time polymerase chain reaction and western blot.Results:The expression of NSD3 was altered in pan-cancer samples.Patients with higher levels of NDS3 generally had shorter overall survival and disease-specific survival.Levels of NSD3 were positively correlated with DNA copy number variation(CNV)in pan-cancer.NSD3 expression was also associated with tumor mutation burden and microsatellite instability.The levels of immune-cell infiltration differed significantly between high and low NSD3 expression.NSD3 negatively correlated with levels of CD8+T cells.Functional enrichment analysis showed that while NSD3 expression was positively associated with several immune cell-related and histone methylation-related pathways,it was negatively correlated with cell metabolism-related,drug transport-related,and drug metabolismrelated pathways.NSD3 levels in the cell lines tested were significantly different.In U251 and NCI-H23 cells,silencing NSD3 inhibited cell proliferation and promoted apoptosis.Conclusions:NSD3 expression was changed in pan-cancer samples that was also verified in cell lines.NSD3 was associated with CNV and immune-cell infiltration.A poor prognosis was predicted in patients with high expression of NSD3.NSD3 might hence be a potential marker for predicting tumor prognosis.展开更多
BACKGROUND:It has been found in recent years that STAT3 widely distributes in nervous system,including hippocampal CA1-3 region,dentate gyrus and cerebral neocortex,etc.Ischemic brain injury can cause the release of s...BACKGROUND:It has been found in recent years that STAT3 widely distributes in nervous system,including hippocampal CA1-3 region,dentate gyrus and cerebral neocortex,etc.Ischemic brain injury can cause the release of some cytokines and growth factors,while electro-acupuncture may have multi-level,multi-channel and multi-target protective and interventional effects on ischemic brain injury.OBJECTIVE:To observe the effects of electro-acupuncture on STAT3 expression and nuclear translocation in hippocampal CA1 region of rat models of brain ischemia/reperfusion.DESIGN:Randomized and controlled observation.SETTING:Staff Room of Acupuncture and Moxibustion,Department of Acupuncture and Bone Injury,Hubei College of Traditional Chinese Medicine;Tongji Medical College,Huazhong University of Science and Technology.MATERIALS:Seventy-two healthy SD rats,of clean degree and either gender,weighing(200±20)g,were provided by the Experimental Animal Center of Hubei College of Traditional Chinese Medicine.STAT3 monoclonal antibody was purchased from Santa Cruz Company,USA,and G-6805 electro-acupuncture instrument was purchased from Shanghai Medical Electronic Instruments Factory.METHODS:This experiment was carried out in the comprehensive laboratory of Department of Acupuncture and Bone Injury,Hubei College of Traditional Chinese Medicine between September 2005 and February 2006.Seventy-two rats were randomly divided into 4 groups:①control group(n=6):Untouched.②Sham-operation group(n=18):Artery was isolated,but without inserting thread bolt.③Model group(n=24):Rat models of local brain ischemia/reperfusion were established with modified suture occlusion.④Electro-acupuncture group(n=24):Dazhui and bilateral Neiguan points were selected for electro-acupuncture treatment.No.28 acupuncture needle of 3.33 cm was used in the treatment.A G-6085 electro-acupuncture instrument with continuous wave,frequency of 120 times/min,intensity of 1 mA,30 min/time,was used.Acupuncture was conducted firstly at ischemia/reperfusion 3 hours,then once every 12 hours.STAT3 positive nuclear translocation in hippocampal CA1 region of rats was observed with immunohistochemical method at 24,48 and 72 hours after brain ishcemia/reperfusion,and then STAT3 positive cells were counted.MAIN OUTCOME MEASURES:STAT3 positive cells and nuclear translocation in hippocampal CA1 region of rats in each group.RESULTS:All the 72 rats were involved in the result analysis.①In the control group and sham-operation group,STAT3 positive cells with light cytoplasm and nucleus were decreased,and nuclear translocation was not found.②In the model group,STAT3 positive cells were mostly found in the cytoplasm of the hippocampal CA1 region at the ischemic side of rats after ischemia/reperfusion 24 hours.They were significantly more than those in the sham-operation group and control group[(18.00±2.68),(9.00±1.35),(8.00±1.22)cells/mm2,P<0.01],but cells with nuclear reaction were fewer;At ischemia/reperfusion 48 and 72 hours,STAT3 positive cells were increased,and they were significantly more than those of sham-operation group[(25.00±3.23),(35.00±3.52)cells/mm2,(13.00±1.93),(12.00±1.24)cells/mm2,P<0.01].Positive cells with nuclear reaction were found dark-stained.③At ischemia/reperfusion 24,48 and 72 hours,STAT3 positive cells were strongly expressed in hippocampal CA1 region at ischemic side of rats of electro-acupuncture group,and they were significantly more than those of model group[(25±3.52),(50±6.31),(75±8.09)cells/mm2,P<0.01].STAT3 positive cells were gradually enhanced with time,and considerable STAT3 nuclear positive reaction cells were found.CONCLUSION:Electro-acupuncture can activate STAT3 protein expression in hippocampal tissue of rats with local brain ischemia/reperfusion,promote STAT3 nuclear translocation and function its neuroprotective effect.展开更多
Wound healing is one of the major global health concerns in patients with diabetes.Overactivation of pro-inflammatory M1 macrophages is associated with delayed wound healing in diabetes.miR-29ab1 plays a critical role...Wound healing is one of the major global health concerns in patients with diabetes.Overactivation of pro-inflammatory M1 macrophages is associated with delayed wound healing in diabetes.miR-29ab1 plays a critical role in diabetes-related macrophage inflammation.Hence,inhibition of inflammation and regulation of miR-29 expression have been implicated as new points for skin wound healing.In this study,the traditional Chinese medicine,puerarin,was introduced to construct an injectable and self-healing chitosan@puerarin(C@P)hydrogel.The C@P hydrogel promoted diabetic wound healing and accelerated angiogenesis,which were related to the inhibition of the miR-29 mediated inflammation response.Compared to healthy subjects,miR-29a and miR-29b1 were ectopically increased in the skin wound of the diabetic model,accompanied by upregulated M1-polarization,and elevated levels of IL-1βand TNF-α.Further evaluations by miR-29ab1 knockout mice exhibited superior wound healing and attenuated inflammation.The present results suggested that miR-29ab1 is essential for diabetic wound healing by regulating the inflammatory response.Suppression of miR-29ab1 by the C@P hydrogel has the potential for improving medical approaches for wound repair.展开更多
基金supported by the National Natural Science Foundation of China,No.30772837the Wuhan Municipal"Morning Sun"Science and Technology Plan,No.200850731347
文摘To examine the possible correlation of aberrant Wnt signaling and pathological changes in Alzheimer’s disease, we established a rat model of Alzheimer’s disease and measured axin and β-catenin expression in the hippocampus. Rats were pretreated with moxibustion or electroacu-puncture, or both, at Baihui（GV20） and Shenshu（BL23）. Axin expression was lower, β-catenin expression was greater, and neuronal cytoplasmic edema was visibly prevented in the rats that had received the pretreatments. Our results suggest that the mechanism underlying the neuro-protective effect of acupuncture and moxibustion in Alzheimer’s disease is associated with axin and β-catenin expression in the Wnt signal transduction pathway.
基金supported in part by the National Natural Science Foundation of China(Grant No.81703005)the Natural Science Foundation of Hunan Province(Grant Nos.2017JJ3195,2018JJ3317)the Key Research and Development Projects of Hunan Province(Grant No.2018SK2120).
文摘Background:Nuclear receptor binding SET domain protein-3(NSD3)is a histone lysine methyltransferase and a crucial regulator of carcinogenesis in several cancers.We aimed to investigate the prognostic value and potential function of NSD3 in 33 types of human cancer.Methods:The data were obtained from The Cancer Genome Atlas.Kaplan-Meier analysis,CIBERSORT,gene set enrichment analysis,and gene set variation analysis were performed.The expression of NSD3 was measured using quantitative real-time polymerase chain reaction and western blot.Results:The expression of NSD3 was altered in pan-cancer samples.Patients with higher levels of NDS3 generally had shorter overall survival and disease-specific survival.Levels of NSD3 were positively correlated with DNA copy number variation(CNV)in pan-cancer.NSD3 expression was also associated with tumor mutation burden and microsatellite instability.The levels of immune-cell infiltration differed significantly between high and low NSD3 expression.NSD3 negatively correlated with levels of CD8+T cells.Functional enrichment analysis showed that while NSD3 expression was positively associated with several immune cell-related and histone methylation-related pathways,it was negatively correlated with cell metabolism-related,drug transport-related,and drug metabolismrelated pathways.NSD3 levels in the cell lines tested were significantly different.In U251 and NCI-H23 cells,silencing NSD3 inhibited cell proliferation and promoted apoptosis.Conclusions:NSD3 expression was changed in pan-cancer samples that was also verified in cell lines.NSD3 was associated with CNV and immune-cell infiltration.A poor prognosis was predicted in patients with high expression of NSD3.NSD3 might hence be a potential marker for predicting tumor prognosis.
基金the Natural Science Foundation of Hubei Province,No.2003ABA154
文摘BACKGROUND:It has been found in recent years that STAT3 widely distributes in nervous system,including hippocampal CA1-3 region,dentate gyrus and cerebral neocortex,etc.Ischemic brain injury can cause the release of some cytokines and growth factors,while electro-acupuncture may have multi-level,multi-channel and multi-target protective and interventional effects on ischemic brain injury.OBJECTIVE:To observe the effects of electro-acupuncture on STAT3 expression and nuclear translocation in hippocampal CA1 region of rat models of brain ischemia/reperfusion.DESIGN:Randomized and controlled observation.SETTING:Staff Room of Acupuncture and Moxibustion,Department of Acupuncture and Bone Injury,Hubei College of Traditional Chinese Medicine;Tongji Medical College,Huazhong University of Science and Technology.MATERIALS:Seventy-two healthy SD rats,of clean degree and either gender,weighing(200±20)g,were provided by the Experimental Animal Center of Hubei College of Traditional Chinese Medicine.STAT3 monoclonal antibody was purchased from Santa Cruz Company,USA,and G-6805 electro-acupuncture instrument was purchased from Shanghai Medical Electronic Instruments Factory.METHODS:This experiment was carried out in the comprehensive laboratory of Department of Acupuncture and Bone Injury,Hubei College of Traditional Chinese Medicine between September 2005 and February 2006.Seventy-two rats were randomly divided into 4 groups:①control group(n=6):Untouched.②Sham-operation group(n=18):Artery was isolated,but without inserting thread bolt.③Model group(n=24):Rat models of local brain ischemia/reperfusion were established with modified suture occlusion.④Electro-acupuncture group(n=24):Dazhui and bilateral Neiguan points were selected for electro-acupuncture treatment.No.28 acupuncture needle of 3.33 cm was used in the treatment.A G-6085 electro-acupuncture instrument with continuous wave,frequency of 120 times/min,intensity of 1 mA,30 min/time,was used.Acupuncture was conducted firstly at ischemia/reperfusion 3 hours,then once every 12 hours.STAT3 positive nuclear translocation in hippocampal CA1 region of rats was observed with immunohistochemical method at 24,48 and 72 hours after brain ishcemia/reperfusion,and then STAT3 positive cells were counted.MAIN OUTCOME MEASURES:STAT3 positive cells and nuclear translocation in hippocampal CA1 region of rats in each group.RESULTS:All the 72 rats were involved in the result analysis.①In the control group and sham-operation group,STAT3 positive cells with light cytoplasm and nucleus were decreased,and nuclear translocation was not found.②In the model group,STAT3 positive cells were mostly found in the cytoplasm of the hippocampal CA1 region at the ischemic side of rats after ischemia/reperfusion 24 hours.They were significantly more than those in the sham-operation group and control group[(18.00±2.68),(9.00±1.35),(8.00±1.22)cells/mm2,P<0.01],but cells with nuclear reaction were fewer;At ischemia/reperfusion 48 and 72 hours,STAT3 positive cells were increased,and they were significantly more than those of sham-operation group[(25.00±3.23),(35.00±3.52)cells/mm2,(13.00±1.93),(12.00±1.24)cells/mm2,P<0.01].Positive cells with nuclear reaction were found dark-stained.③At ischemia/reperfusion 24,48 and 72 hours,STAT3 positive cells were strongly expressed in hippocampal CA1 region at ischemic side of rats of electro-acupuncture group,and they were significantly more than those of model group[(25±3.52),(50±6.31),(75±8.09)cells/mm2,P<0.01].STAT3 positive cells were gradually enhanced with time,and considerable STAT3 nuclear positive reaction cells were found.CONCLUSION:Electro-acupuncture can activate STAT3 protein expression in hippocampal tissue of rats with local brain ischemia/reperfusion,promote STAT3 nuclear translocation and function its neuroprotective effect.
基金supported by grants from the National Natural Science Foundation of China(32071344,32000938,81974326,81403029)Natural Science Foundation of Shanghai(19ZR1449100)+1 种基金Science and Technology Commission of Shanghai Municipality(19JC1415500)S&T Innovation 2025 Major Special Program of Ningbo(2019B10063).
文摘Wound healing is one of the major global health concerns in patients with diabetes.Overactivation of pro-inflammatory M1 macrophages is associated with delayed wound healing in diabetes.miR-29ab1 plays a critical role in diabetes-related macrophage inflammation.Hence,inhibition of inflammation and regulation of miR-29 expression have been implicated as new points for skin wound healing.In this study,the traditional Chinese medicine,puerarin,was introduced to construct an injectable and self-healing chitosan@puerarin(C@P)hydrogel.The C@P hydrogel promoted diabetic wound healing and accelerated angiogenesis,which were related to the inhibition of the miR-29 mediated inflammation response.Compared to healthy subjects,miR-29a and miR-29b1 were ectopically increased in the skin wound of the diabetic model,accompanied by upregulated M1-polarization,and elevated levels of IL-1βand TNF-α.Further evaluations by miR-29ab1 knockout mice exhibited superior wound healing and attenuated inflammation.The present results suggested that miR-29ab1 is essential for diabetic wound healing by regulating the inflammatory response.Suppression of miR-29ab1 by the C@P hydrogel has the potential for improving medical approaches for wound repair.
