Objective:This study aimed to assess the feasibility and safety of the SHURUI single-port robotic surgical system for a range of major urological surgeries.Methods:In this prospective,multicenter clinical trial,we exa...Objective:This study aimed to assess the feasibility and safety of the SHURUI single-port robotic surgical system for a range of major urological surgeries.Methods:In this prospective,multicenter clinical trial,we examined the effectiveness of the SHURUI single-port robotic surgical system in urological interventions.The first 50 patients from four centers in China underwent single-port surgeries including partial nephrectomy,radical prostatectomy,partial adrenalectomy,and pyeloureteroplasty,exclusively by the SHURUI single-port robotic surgical system.The study's primary endpoints focused on the success of surgeries,defined as no deviations from planned procedures,no need for more than one port,and no re-operations within 24 h after surgery.Secondary endpoints encompassed a range of surgical metrics,functional outcomes,and patient demographic data.Clinical assessments were conducted before surgery,before discharge,and 1 month after discharge.Results:The surgical procedures were executed successfully without requiring intraoperative conversions or transfusions.Both estimated blood loss and operation durations were maintained within satisfactory limits.For each type of surgery,the mean console times and estimated blood loss were 179.8(standard deviation[SD]39.4)min and 125.6(SD 126.0)mL for radical prostatectomy,126.7(SD 47.8)min and 39.2(SD 54.4)mL for partial nephrectomy,112.6(SD 37.4)min and 20.0(SD 13.2)mL for partial adrenalectomy,and 148.0(SD 18.2)min and 18.0(SD 17.9)mL for pyeloureteroplasty,respectively.Across the cohort,17 patients experienced a total of 25 adverse events,while 10 postoperative complications,all rated as Clavien-Dindo grade I,were encountered by eight patients.All patients had shown recovery or improvement from these events before the end of this trial.Conclusion:The SHURUI single-port robotic surgical system demonstrated feasibility and safety in the performance of major urological surgeries.These initial findings highlight the system's potential,though further research and longer follow-up are required to assess long-term outcomes.展开更多
Ureteral stricture formation after ureteroscopic lithotripsy is a late complication that can lead to hydronephrosis and a subsequent risk of renal deterioration.The specific incidence is unknown,and the mechanism of s...Ureteral stricture formation after ureteroscopic lithotripsy is a late complication that can lead to hydronephrosis and a subsequent risk of renal deterioration.The specific incidence is unknown,and the mechanism of stricture formation has not been completely explained.In this review,we summarize the current evidence regarding the incidence of this condition and discuss its pathogenesis.We then list preventive strategies to reduce the morbidity of ureteral strictures。展开更多
Microcystis panniformis is a bloom forming species with flat panniform-like colonies. This species was recently found in Lake Taihu, China. To specifically characterize M. panniformis based on isolated strains, morpho...Microcystis panniformis is a bloom forming species with flat panniform-like colonies. This species was recently found in Lake Taihu, China. To specifically characterize M. panniformis based on isolated strains, morphological examination on colonial transition and genetic examination are needed. Three M. panniformis strains isolated from a water bloom sample in Lake Taihu were characterized by molecular analysis and toxin quantification. Phylogenetic analysis based on both 16S rRNA gene and internal transcribed spacer(ITS) between 16S and 23S rRNA genes were performed and compared to facilitate easy identification of the species.Relatively high similarities(98%–99%) were shown in 16S rDNA sequences between the strains of M. panniformis and those of other Microcystis species, whereas the similarities for ITS sequences were 88%–95%. In the phylogenetic tree based on the 16S rDNA sequences, the M. panniformis and M. aeruginosa strains were intermixed together with no clear division,whereas all of the M. panniformis strains were clustered together in a single clade based on the ITS sequences based phylogenyetic tree. The mcyE gene was detected in all three strains, and microcystin was determined by high-performance liquid chromatography. The molecular detection and toxin production of M. panniformis strains are of great significance for the environmental risk assessment of Microcystis blooms.展开更多
Objective:To describe the Chinese experience of natural orifice transluminal endoscopic surgery(NOTES)in urology.Methods:From December 2008 to May 2017,35 animal experiments and 305 clinical surgeries of NOTES or natu...Objective:To describe the Chinese experience of natural orifice transluminal endoscopic surgery(NOTES)in urology.Methods:From December 2008 to May 2017,35 animal experiments and 305 clinical surgeries of NOTES or natural orifices specimen extractions(NOSE)were performed in China.The animal experiments included five kidney biopsies,24 nephrectomies and six partial nephrectomies.The clinical surgeries included 12 transvaginal NOSE(TV-NOSE),266 hybrid transvaginal NOTES(TV-NOTES)and 27 pure TV-NOTES.The TV-NOSE procedure was performed in five transumbilical laparoendoscopic single-site(U-LESS)nephrectomies,four suprapubic-assisted laparoendoscopic single-site surgery(SA-LESS)nephroureterectomies,and three laparoscopic radical cystectomies.The hybrid TV-NOTES procedure included 210 nephrectomies,31 adrenalectomies,eight nephroureterectomies,13 partial nephrectomies,and four heminephrectomies.The pure TV-NOTES procedure included five renal cyst decortications and 22 nephrectomies.Results:A total of 29 animal experiments were successfully performed.One partial nephrectomy was converted to standard laparoscopic surgery.Two kidney biopsies and two nephrectomies were unsuccessful.A total of 297 clinical surgeries were successfully performed.Six patients who underwent hybrid TV-NOTES were converted to open surgery.Two patients who underwent pure TV-NOTES were converted to SA-LESS.There were 22 major complications,16 occurred intraoperatively and six postoperatively.The mean visual analog score(VAS)of 48 h after the operation was 2.5 points in TV-NOSE,2.3 points in hybrid TV-NOTES and 1.7 points in pure TV-NOTES.The mean follow-up of 50.6(3.0-87.0)months showed that all patients were in good condition.The umbilicus scars were nearly invisible in TV-NOSE and hybrid TV-NOTES.The vaginal incision healed well.Conclusions:TV-NOSE and TV-NOTES are feasible,safe,and effective with little injury,low pain,fast recovery,and good cosmetic outcomes in properly selected patients.They are worth consideration for urological clinical practice.展开更多
Staghorn stones have always been a challenge for urologists,especially in some special situations,such as horseshoe kidney,ectopic kidney,paediatric kidney,and solitary kidney.The treatment of these staghorn stones mu...Staghorn stones have always been a challenge for urologists,especially in some special situations,such as horseshoe kidney,ectopic kidney,paediatric kidney,and solitary kidney.The treatment of these staghorn stones must be aggressive because they can lead to renal function loss and serious complications.The gold-standard management for staghorn stones is surgical treatment with the aim of clearing the stones and preserving renal function.Treatment methods for staghorn stones have developed rapidly,such as extracorporeal shock wave lithotripsy,retrograde intrarenal surgery,percutaneous nephrolithotomy and laparoscopy and open surgery.Whether the standard procedures for staghorn stones can also apply to these stones in special situations is still not agreed upon.The decision should be made individually according to the circumstances of the patient.In this review,we evaluates the previous studies and comments on the management of staghorn stones under special situations in the hope of guiding the optimal choice for urologists.展开更多
Background:IQGAP3 plays a crucial role in regulating cell proliferation,division,and cytoskeletal organization.Abnormal expression of IQGAP3 has been linked to various tumors,but its function in glioma is not well und...Background:IQGAP3 plays a crucial role in regulating cell proliferation,division,and cytoskeletal organization.Abnormal expression of IQGAP3 has been linked to various tumors,but its function in glioma is not well understood.Methods:Various methods,including genetic differential analysis,single-cell analysis,ROC curve analysis,Cox regression,Kaplan-Meier analysis,and enrichment analysis,were employed to analyze the expression patterns,diagnostic potential,prognostic implications,and biological processes involving IQGAP3 in normal and tumor tissues.The impact of IQGAP3 on immune infiltration and the immune microenvironment in gliomas was evaluated using immunofluorescence.Additionally,the cBioPortal database was used to analyze copy number variations and mutation sites of IQGAP3.Experimental validation was also performed to assess the effects of IQGAP3 on glioma cells and explore underlying mechanisms.Results:High IQGAP3 expression in gliomas is associated with an unfavorable prognosis,particularly in wild-type IDH and 1p/19q non-codeleted gliomas.Enrichment analysis revealed that IQGAP3 is involved in regulating the cell cycle,PI3K/AKT signaling,p53 signaling,and PLK1-related pathways.Furthermore,IQGAP3 expression may be closely related to the immunosuppressive microenvironment of glioblastoma.BRD-K88742110 and LY-303511 are potential drugs for targeting IQGAP3 in anti-glioma therapy.In vitro experiments showed that downregulation of IQGAP3 inhibits the proliferation and migration of glioma cells,with the PLK1/PI3K/AKT pathway potentially playing a crucial role in IQGAP3-mediated glioma progression.Conclusion:IQGAP3 shows promise as a valuable biomarker for diagnosis,prognosis,and immunotherapeutic strategies in gliomas.展开更多
In the present work, core-shell Ni@SiO2 catalysts were investigated in order to evaluate the relevance of catalytic activity and surface states of Ni core as well as Ni nanoparticles size to catalytic partial oxidatio...In the present work, core-shell Ni@SiO2 catalysts were investigated in order to evaluate the relevance of catalytic activity and surface states of Ni core as well as Ni nanoparticles size to catalytic partial oxidation of methane （POM）. The catalysts were characterized by N2 adsorption, H2-TPR, XRD, TEM and XPS techniques. The catalytic performance of the core-shell catalysts was found to be dependent on the surface states of catalyst, which influenced the formation of products. It was considered that carbon dioxide formed on the oxidized nickel sites （NiO） and carbon monoxide produced on the reduced sites （Ni）. The surface states of active metal in the dynamic were influenced both by the size of Ni core and the porosity of silica shell. However, the catalytic activity would be debased when the size of Ni core was under a certain extent, which can be ascribed to the fact the carbon deposition increased with the increasing content of NiO. The effects of surface states of Ni@SiO2 catalyst on the catalytic performance were discussed and the reaction pathway over Ni core encapsulated inside silica shell was proposed.展开更多
Objective:Complex ureteral obstruction is refractory to conventional urological intervention.This report describes a case of laparoscopic ureterolysis with simultaneous ureteroscopy and percutaneous nephroscopy for tr...Objective:Complex ureteral obstruction is refractory to conventional urological intervention.This report describes a case of laparoscopic ureterolysis with simultaneous ureteroscopy and percutaneous nephroscopy for treating complex ureteral obstruction.Methods:Right-side multiple ureteral stones and complicating ureteral obstruction failed an initial attempt of ureteroscopy lithotripsy with simultaneous percutaneous nephroscopy in a 23-year-old male.Laparoscopic ureterolysis with ureteroscopy and percutaneous nephroscopy was used simultaneously to dissect the periureteral adhesions with the patient placed in the Galdakao-modified supine Valdivia position.The ureter was incised to allow the insertion of a ureteral catheter through the twisted ureter,and a guide wire was advanced into the pelvis using ureteroscopy.A double-J stent was placed into the right-side ureter using antegrade percutaneous nephroscopy.Results:The laparoendoscopic procedure lasted 330 min with an estimated bleeding volume of 100 mL.The patient underwent an uneventful postoperative course,and postoperative followup radiography confirmed good positioning of the double-J stent.The double-J stent was removed 3 months after operation.The patient remained asymptomatic within a 13-month follow-up period.Conclusion:Laparoscopic ureterolysis with simultaneous ureteroscopy and percutaneous nephroscopy is an effective and safe treatment option for complex ureteral obstruction.展开更多
Lessening energy-related carbon emissions has become a crucial measure to achieve Chinese carbon neutrality.This study is the first to construct a Difference in Carbon pressures-adjusted Human Development Index(DCHDI)...Lessening energy-related carbon emissions has become a crucial measure to achieve Chinese carbon neutrality.This study is the first to construct a Difference in Carbon pressures-adjusted Human Development Index(DCHDI)model for the purpose of exploring the coupling effect between carbon emissions and human development variety from 2000 to 2019 in Chinese provinces.We demonstrate the following.(1)The total energy-related carbon footprint of 30 provinces in China reached 10.2 billion tons in 2019,with an average annual growth rate of 6.93% over the past two decades;and the provinces with the highest carbon emissions per capita are InnerMongolia,Ningxia,and Shanxi.(2)At the provincial level,we observed that the Human Development Index(HDI),which includes life expectancy,education,and income,has been rising,while Beijing,Shanghai,and Tianjin entered the super-high HDI level before 2008.(3)The entire coupling effect of 30 Chinese provinces has been broadly fortified in the last 20 years,but the growth rate of DCHDI values in 2011-2019 has slowed down compared with that in 2000-2010;the clustering phenomenon demonstrated that this discovery is associated with historical peaks in total carbon emissions.(4)The co-ordination degree of carbon emissions per capita and HDI was verified,and 96% of the data points were found in the range of super high coupling coordination degree.Overall,this study provides the government with worthwhile guidance for decision-making and carbon reduction strategies for other countries struggling to advance human sustainable development.展开更多
Three transition metal-like facet centered cubic structured transition metal nitrides,γ-Mo_(2)N,β-W_(2)N andδ-NbN,are synthesized and applied in the reaction of CO_(2)hydrogenation to CO.Among the three nitride cat...Three transition metal-like facet centered cubic structured transition metal nitrides,γ-Mo_(2)N,β-W_(2)N andδ-NbN,are synthesized and applied in the reaction of CO_(2)hydrogenation to CO.Among the three nitride catalysts,theγ-Mo_(2)N exhibits superior activity to target product CO,which is 4.6 and 76 times higher than the other two counterparts ofβ-W_(2)N andδ-NbN at 600℃,respectively.Additionally,γ-Mo_(2)N exhibits excellent stability on both cyclic heating-cooling and high space velocity steady state operation.The deactivation degree of cyclic heating-cooling evaluation after 5 cycles and long-term stability performance at 773 and 873 K in 50 h are all less than 10%.In-situ XRD and kinetic studies suggest that theγ-Mo_(2)N itself is able to activate both of the reactants CO_(2)and H_(2).Below 400℃,the reaction mainly occurs at the surface ofγ-Mo_(2)N catalyst.CO_(2)and H_(2)competitively adsorbe on the surface of catalyst and CO_(2)is the relatively stronger surface adsorbate.At a higher temperature,the interstitial vacancies of theγ-Mo_(2)N can be reversibly filled with the oxygen from CO_(2)dissociation.Both of the surface and bulk phase sites ofγ-Mo_(2)N participate in the high temperature CO_(2)hydrogenation pathway.展开更多
Human health is threatened by foodborne illness and Staphylococcus aureus is a common foodborne pathogenic bacteria.It can cause food poisoning when we are infected,therefore,it is necessary to detect pathogenic bacte...Human health is threatened by foodborne illness and Staphylococcus aureus is a common foodborne pathogenic bacteria.It can cause food poisoning when we are infected,therefore,it is necessary to detect pathogenic bacteria.The virulence genes and detection methods of S.aureus are summarized from literatures.Traditional detection methods are simple but need bacteria enrichment and to prolong detection time.Immunological technology has high specificity,but false positive results are easy to occur in its detection.In recent years,molecular biology methods have developed rapidly,and various PCR techniques have been applied to detect foodborne pathogens.Real-time fluorescent quantitative PCR is more effective than ordinary PCR,while price is expensive when use it,the scope of use is limited.LAMP is a flexible detection method,biosensor and flow cytometry are fast but the cost of detection is high and the price is relatively expensive.展开更多
Based on the analysis of the current situation and existing problems of traditional Chinese soft shelled turtle(Trionyx sinensis)breeding industry,combined with the fact that selenium enriched functional agriculture i...Based on the analysis of the current situation and existing problems of traditional Chinese soft shelled turtle(Trionyx sinensis)breeding industry,combined with the fact that selenium enriched functional agriculture is the future demand of mankind,this paper put forward the necessity of developing selenium enriched Chinese soft shelled turtles and promoting the sustainable development of modern agriculture:the development of selenium-enriched Chinese soft-shelled turtle is the need of the consumer market for food safety,the need to reduce the production cost of soft-shelled turtles and improve the production efficiency of soft-shelled turtles,the need to enrich the Chinese soft-shelled turtle market,and the need to promote the sustainable development of modern agriculture(fishery).展开更多
The fracture risk of patients with diabetes is higher than those of patients without diabetes due to hyperglycemia,usage of diabetes drugs,changes in insulin levels,and excretion,and this risk begins as early as adole...The fracture risk of patients with diabetes is higher than those of patients without diabetes due to hyperglycemia,usage of diabetes drugs,changes in insulin levels,and excretion,and this risk begins as early as adolescence.Many factors including demographic data(such as age,height,weight,and gender),medical history(such as smoking,drinking,and menopause),and examination(such as bone mineral density,blood routine,and urine routine)may be related to bone metabolism in patients with diabetes.However,most of the existing methods are qualitative assessments and do not consider the interactions of the physiological factors of humans.In addition,the fracture risk of patients with diabetes and osteoporosis has not been further studied previously.In this paper,a hybrid model combining XGBoost with deep neural network is used to predict the fracture risk of patients with diabetes and osteoporosis,and investigate the effect of patients’physiological factors on fracture risk.A total of 147 raw input features are considered in our model.The presented model is compared with several benchmarks based on various metrics to prove its effectiveness.Moreover,the top 18 influencing factors of fracture risks of patients with diabetes are determined.展开更多
Perovskite solar cells(PSCs)are undergoing rapid development and the power conversion efficiency reaches 25.7%which attracts increasing attention on their commercialization recently.In this review,we summarized the re...Perovskite solar cells(PSCs)are undergoing rapid development and the power conversion efficiency reaches 25.7%which attracts increasing attention on their commercialization recently.In this review,we summarized the recent progress of PSCs based on device structures,perovskite-based tandem cells,large-area modules,stability,applications and industrialization.Last,the challenges and perspectives are discussed,aiming at providing a thrust for the commercialization of PSCs in the near future.展开更多
Exploration of heavy metals and organic pollutants, their leaching capacity along with health and environmental risks in contaminated industrial construction and demolition waste （ICDW） within a pesticide manufactu...Exploration of heavy metals and organic pollutants, their leaching capacity along with health and environmental risks in contaminated industrial construction and demolition waste （ICDW） within a pesticide manufacturing plant were investigated. A maximum content of 90.8 mg·kg-1 Cd was found present in the wastes, which might originate from phosphorus rocks and industrial sulfuric acid used in pesticide production processes. An average concentration of 979.8 mg·kg-1 dichlorovos and other 11 organophosphorus pesticide were also detected. Relatively high leaching rates of around 4.14‰ were obtained from laboratory simulated ICDW using both glacial acetic acidsodium hydroxide and deionized water. Pesticide pollutants had the strongest tendency to retaining on dry bricks （leaching rate 1.68 9‰） compared to mortar-coatings, etc. due to their different physical characteristics and octanol-water partioning coefficient. Mobility of pesticide from on-site ICDW by water was spatially correlated to waste types, process sections and human activities, with a flux of leaching rate between 5.9‰ to 27.4%. Risk-based corrective action （RBCA） model was used to simulate the risk of contaminated ICDW debris randomly scattered. Oral and demlal ingestion amount by local workers was 9.8 × 10-3 and 1.9 × 10-2 mg.（kg-d）-1, respectively. Potential leaching risk to aquatic systems exceeded the limit for nearly 75% waste. Environmental and health risk exceedance was found in most ICDW, while the risk value of the most severely contaminated brick waste was 660 times beyond critical level. Implications for waste management involving construction and deconstruction work, waste transferring and regulation supplying were also provided.展开更多
Concrete is the most commonly used construction material.However,its production leads to high carbon dioxide(CO_(2))emissions and energy consumption.Therefore,developing waste-substitutable concrete components is nece...Concrete is the most commonly used construction material.However,its production leads to high carbon dioxide(CO_(2))emissions and energy consumption.Therefore,developing waste-substitutable concrete components is necessary.Improving the sustainability and greenness of concrete is the focus of this research.In this regard,899 data points were collected from existing studies where cement,slag,fly ash,superplasticizer,coarse aggregate,and fine aggregate were considered potential influential factors.The complex relationship between influential factors and concrete compressive strength makes the prediction and estimation of compressive strength difficult.Instead of the traditional compressive strength test,this study combines five novel metaheuristic algorithms with extreme gradient boosting(XGB)to predict the compressive strength of green concrete based on fly ash and blast furnace slag.The intelligent prediction models were assessed using the root mean square error(RMSE),coefficient of determination(R^(2)),mean absolute error(MAE),and variance accounted for(VAF).The results indicated that the squirrel search algorithm-extreme gradient boosting(SSA-XGB)yielded the best overall prediction performance with R^(2) values of 0.9930 and 0.9576,VAF values of 99.30 and 95.79,MAE values of 0.52 and 2.50,RMSE of 1.34 and 3.31 for the training and testing sets,respectively.The remaining five prediction methods yield promising results.Therefore,the developed hybrid XGB model can be introduced as an accurate and fast technique for the performance prediction of green concrete.Finally,the developed SSA-XGB considered the effects of all the input factors on the compressive strength.The ability of the model to predict the performance of concrete with unknown proportions can play a significant role in accelerating the development and application of sustainable concrete and furthering a sustainable economy.展开更多
基金funded by the National Key Research and Development Program of China(Grant No.2022YFB4700904 to Wang L)the Shanghai Shenkang Hospital Development Center's project for the Promotion of Clinical Skills and Clinical Innovation Three-Year Action Plan(Project No.SHDC2022CRT006 to Wang L and SHDC2022CRS010B to Tang S).
文摘Objective:This study aimed to assess the feasibility and safety of the SHURUI single-port robotic surgical system for a range of major urological surgeries.Methods:In this prospective,multicenter clinical trial,we examined the effectiveness of the SHURUI single-port robotic surgical system in urological interventions.The first 50 patients from four centers in China underwent single-port surgeries including partial nephrectomy,radical prostatectomy,partial adrenalectomy,and pyeloureteroplasty,exclusively by the SHURUI single-port robotic surgical system.The study's primary endpoints focused on the success of surgeries,defined as no deviations from planned procedures,no need for more than one port,and no re-operations within 24 h after surgery.Secondary endpoints encompassed a range of surgical metrics,functional outcomes,and patient demographic data.Clinical assessments were conducted before surgery,before discharge,and 1 month after discharge.Results:The surgical procedures were executed successfully without requiring intraoperative conversions or transfusions.Both estimated blood loss and operation durations were maintained within satisfactory limits.For each type of surgery,the mean console times and estimated blood loss were 179.8(standard deviation[SD]39.4)min and 125.6(SD 126.0)mL for radical prostatectomy,126.7(SD 47.8)min and 39.2(SD 54.4)mL for partial nephrectomy,112.6(SD 37.4)min and 20.0(SD 13.2)mL for partial adrenalectomy,and 148.0(SD 18.2)min and 18.0(SD 17.9)mL for pyeloureteroplasty,respectively.Across the cohort,17 patients experienced a total of 25 adverse events,while 10 postoperative complications,all rated as Clavien-Dindo grade I,were encountered by eight patients.All patients had shown recovery or improvement from these events before the end of this trial.Conclusion:The SHURUI single-port robotic surgical system demonstrated feasibility and safety in the performance of major urological surgeries.These initial findings highlight the system's potential,though further research and longer follow-up are required to assess long-term outcomes.
基金Supported by the National Natural Science Foundation of China(31171717)by the National Programs for High Technology Research and Development of China(2012AA022108)by the Synergy Innovation Center of Food Safety and Nutrition of China
文摘Ureteral stricture formation after ureteroscopic lithotripsy is a late complication that can lead to hydronephrosis and a subsequent risk of renal deterioration.The specific incidence is unknown,and the mechanism of stricture formation has not been completely explained.In this review,we summarize the current evidence regarding the incidence of this condition and discuss its pathogenesis.We then list preventive strategies to reduce the morbidity of ureteral strictures。
基金supported by the National Water Science and Technology Project (No.2017ZX07203-003)the National Basic Research Program of China (No.2017YFA0605201)the support from the National Natural Science Foundation of China (No.412111020)
文摘Microcystis panniformis is a bloom forming species with flat panniform-like colonies. This species was recently found in Lake Taihu, China. To specifically characterize M. panniformis based on isolated strains, morphological examination on colonial transition and genetic examination are needed. Three M. panniformis strains isolated from a water bloom sample in Lake Taihu were characterized by molecular analysis and toxin quantification. Phylogenetic analysis based on both 16S rRNA gene and internal transcribed spacer(ITS) between 16S and 23S rRNA genes were performed and compared to facilitate easy identification of the species.Relatively high similarities(98%–99%) were shown in 16S rDNA sequences between the strains of M. panniformis and those of other Microcystis species, whereas the similarities for ITS sequences were 88%–95%. In the phylogenetic tree based on the 16S rDNA sequences, the M. panniformis and M. aeruginosa strains were intermixed together with no clear division,whereas all of the M. panniformis strains were clustered together in a single clade based on the ITS sequences based phylogenyetic tree. The mcyE gene was detected in all three strains, and microcystin was determined by high-performance liquid chromatography. The molecular detection and toxin production of M. panniformis strains are of great significance for the environmental risk assessment of Microcystis blooms.
基金the National High Technology Research and Development Program(“863”Program)of China(2012AA021100)Ganpo 555 Talents Program of Jiangxi Province+2 种基金the Major Science and Technology Support Project from the Department of Science and Technology of Jiangxi Province(20132BAB205007)the Science and Technology Floor Project from the Department of Education of Jiangxi Province(KJLD12044)the Science and Technology Program from the Department of Health of Jiangxi Province(20121095).
文摘Objective:To describe the Chinese experience of natural orifice transluminal endoscopic surgery(NOTES)in urology.Methods:From December 2008 to May 2017,35 animal experiments and 305 clinical surgeries of NOTES or natural orifices specimen extractions(NOSE)were performed in China.The animal experiments included five kidney biopsies,24 nephrectomies and six partial nephrectomies.The clinical surgeries included 12 transvaginal NOSE(TV-NOSE),266 hybrid transvaginal NOTES(TV-NOTES)and 27 pure TV-NOTES.The TV-NOSE procedure was performed in five transumbilical laparoendoscopic single-site(U-LESS)nephrectomies,four suprapubic-assisted laparoendoscopic single-site surgery(SA-LESS)nephroureterectomies,and three laparoscopic radical cystectomies.The hybrid TV-NOTES procedure included 210 nephrectomies,31 adrenalectomies,eight nephroureterectomies,13 partial nephrectomies,and four heminephrectomies.The pure TV-NOTES procedure included five renal cyst decortications and 22 nephrectomies.Results:A total of 29 animal experiments were successfully performed.One partial nephrectomy was converted to standard laparoscopic surgery.Two kidney biopsies and two nephrectomies were unsuccessful.A total of 297 clinical surgeries were successfully performed.Six patients who underwent hybrid TV-NOTES were converted to open surgery.Two patients who underwent pure TV-NOTES were converted to SA-LESS.There were 22 major complications,16 occurred intraoperatively and six postoperatively.The mean visual analog score(VAS)of 48 h after the operation was 2.5 points in TV-NOSE,2.3 points in hybrid TV-NOTES and 1.7 points in pure TV-NOTES.The mean follow-up of 50.6(3.0-87.0)months showed that all patients were in good condition.The umbilicus scars were nearly invisible in TV-NOSE and hybrid TV-NOTES.The vaginal incision healed well.Conclusions:TV-NOSE and TV-NOTES are feasible,safe,and effective with little injury,low pain,fast recovery,and good cosmetic outcomes in properly selected patients.They are worth consideration for urological clinical practice.
基金This work was financed by a grant from the National Natural Science Foundation of China(No.81800624).
文摘Staghorn stones have always been a challenge for urologists,especially in some special situations,such as horseshoe kidney,ectopic kidney,paediatric kidney,and solitary kidney.The treatment of these staghorn stones must be aggressive because they can lead to renal function loss and serious complications.The gold-standard management for staghorn stones is surgical treatment with the aim of clearing the stones and preserving renal function.Treatment methods for staghorn stones have developed rapidly,such as extracorporeal shock wave lithotripsy,retrograde intrarenal surgery,percutaneous nephrolithotomy and laparoscopy and open surgery.Whether the standard procedures for staghorn stones can also apply to these stones in special situations is still not agreed upon.The decision should be made individually according to the circumstances of the patient.In this review,we evaluates the previous studies and comments on the management of staghorn stones under special situations in the hope of guiding the optimal choice for urologists.
基金supported by the Doctoral Foundation of HuBei University of Science and Technology(Grant Numbers BK202007 and BK202028 to L.W.and Z.Z.)Special Research Fund Project of School of Stomatology and Optometry,Xianning Medical College,Hubei University of Science and Technology(Grant Number 2020XZ37 to L.W.)+3 种基金Hubei Provincial Department of Education“Hundred Schools and Hundred Counties”(Grant Number BXLBX0806 to Z.Z.)the Foundation of Hubei University of Science and Technology“Double Hundred Project”(Grant Number 2022HKSB01 to Z.Z.)the Foundation of Innovation Team of Hubei University of Science and Technology(Grant Number 2023T13 to S.Y.)Natural Science Foundation of Hubei Province(Grant Number 2023AFB1027 to Z.Z.).
文摘Background:IQGAP3 plays a crucial role in regulating cell proliferation,division,and cytoskeletal organization.Abnormal expression of IQGAP3 has been linked to various tumors,but its function in glioma is not well understood.Methods:Various methods,including genetic differential analysis,single-cell analysis,ROC curve analysis,Cox regression,Kaplan-Meier analysis,and enrichment analysis,were employed to analyze the expression patterns,diagnostic potential,prognostic implications,and biological processes involving IQGAP3 in normal and tumor tissues.The impact of IQGAP3 on immune infiltration and the immune microenvironment in gliomas was evaluated using immunofluorescence.Additionally,the cBioPortal database was used to analyze copy number variations and mutation sites of IQGAP3.Experimental validation was also performed to assess the effects of IQGAP3 on glioma cells and explore underlying mechanisms.Results:High IQGAP3 expression in gliomas is associated with an unfavorable prognosis,particularly in wild-type IDH and 1p/19q non-codeleted gliomas.Enrichment analysis revealed that IQGAP3 is involved in regulating the cell cycle,PI3K/AKT signaling,p53 signaling,and PLK1-related pathways.Furthermore,IQGAP3 expression may be closely related to the immunosuppressive microenvironment of glioblastoma.BRD-K88742110 and LY-303511 are potential drugs for targeting IQGAP3 in anti-glioma therapy.In vitro experiments showed that downregulation of IQGAP3 inhibits the proliferation and migration of glioma cells,with the PLK1/PI3K/AKT pathway potentially playing a crucial role in IQGAP3-mediated glioma progression.Conclusion:IQGAP3 shows promise as a valuable biomarker for diagnosis,prognosis,and immunotherapeutic strategies in gliomas.
基金supported by the Project of "Utilization of Low Rank Coal" Strategic Leading Special Fund.Chinese Academy of Sciences(XDA07070800)the Program of Overseas Science and Technology Activity(2012-35) and Shanxi Province and the Cooperation Program from Shanxi Fenxi Mining(Group)Co.,Ltd
文摘In the present work, core-shell Ni@SiO2 catalysts were investigated in order to evaluate the relevance of catalytic activity and surface states of Ni core as well as Ni nanoparticles size to catalytic partial oxidation of methane （POM）. The catalysts were characterized by N2 adsorption, H2-TPR, XRD, TEM and XPS techniques. The catalytic performance of the core-shell catalysts was found to be dependent on the surface states of catalyst, which influenced the formation of products. It was considered that carbon dioxide formed on the oxidized nickel sites （NiO） and carbon monoxide produced on the reduced sites （Ni）. The surface states of active metal in the dynamic were influenced both by the size of Ni core and the porosity of silica shell. However, the catalytic activity would be debased when the size of Ni core was under a certain extent, which can be ascribed to the fact the carbon deposition increased with the increasing content of NiO. The effects of surface states of Ni@SiO2 catalyst on the catalytic performance were discussed and the reaction pathway over Ni core encapsulated inside silica shell was proposed.
基金supported by grants from the Shanghai Municipal Hospitals’Project for Emerging and Frontier Technology(No.SHDC12010115)Chinese Military Major Project for Clinical High-tech and Innovative Technology(No.2010gxjs057)the Project for the Key Discipline of Shanghai(No.2013046).
文摘Objective:Complex ureteral obstruction is refractory to conventional urological intervention.This report describes a case of laparoscopic ureterolysis with simultaneous ureteroscopy and percutaneous nephroscopy for treating complex ureteral obstruction.Methods:Right-side multiple ureteral stones and complicating ureteral obstruction failed an initial attempt of ureteroscopy lithotripsy with simultaneous percutaneous nephroscopy in a 23-year-old male.Laparoscopic ureterolysis with ureteroscopy and percutaneous nephroscopy was used simultaneously to dissect the periureteral adhesions with the patient placed in the Galdakao-modified supine Valdivia position.The ureter was incised to allow the insertion of a ureteral catheter through the twisted ureter,and a guide wire was advanced into the pelvis using ureteroscopy.A double-J stent was placed into the right-side ureter using antegrade percutaneous nephroscopy.Results:The laparoendoscopic procedure lasted 330 min with an estimated bleeding volume of 100 mL.The patient underwent an uneventful postoperative course,and postoperative followup radiography confirmed good positioning of the double-J stent.The double-J stent was removed 3 months after operation.The patient remained asymptomatic within a 13-month follow-up period.Conclusion:Laparoscopic ureterolysis with simultaneous ureteroscopy and percutaneous nephroscopy is an effective and safe treatment option for complex ureteral obstruction.
基金supported by the National Key R&D Program of China(No.2018YFD1100203)the National Natural Science Foundation of China(No.52200208)the International Postdoctoral Exchange Fellowship Program(No.YJ20200280)。
文摘Lessening energy-related carbon emissions has become a crucial measure to achieve Chinese carbon neutrality.This study is the first to construct a Difference in Carbon pressures-adjusted Human Development Index(DCHDI)model for the purpose of exploring the coupling effect between carbon emissions and human development variety from 2000 to 2019 in Chinese provinces.We demonstrate the following.(1)The total energy-related carbon footprint of 30 provinces in China reached 10.2 billion tons in 2019,with an average annual growth rate of 6.93% over the past two decades;and the provinces with the highest carbon emissions per capita are InnerMongolia,Ningxia,and Shanxi.(2)At the provincial level,we observed that the Human Development Index(HDI),which includes life expectancy,education,and income,has been rising,while Beijing,Shanghai,and Tianjin entered the super-high HDI level before 2008.(3)The entire coupling effect of 30 Chinese provinces has been broadly fortified in the last 20 years,but the growth rate of DCHDI values in 2011-2019 has slowed down compared with that in 2000-2010;the clustering phenomenon demonstrated that this discovery is associated with historical peaks in total carbon emissions.(4)The co-ordination degree of carbon emissions per capita and HDI was verified,and 96% of the data points were found in the range of super high coupling coordination degree.Overall,this study provides the government with worthwhile guidance for decision-making and carbon reduction strategies for other countries struggling to advance human sustainable development.
基金financially supported by the National Natural Science Foundation of China(22002140)Zhejiang Provincial Natural Science Foundation of China(LR21B030001 and LR22b030003)+1 种基金Young Elite Scientist Sponsorship Program by CAST(No.2019QNRC001)Use of the Advanced Photon Source(beamlines 17-BM,for in-situ XRD characterization)was supported by the U.S.DOE under contract no.DE-AC02-06CH11357。
文摘Three transition metal-like facet centered cubic structured transition metal nitrides,γ-Mo_(2)N,β-W_(2)N andδ-NbN,are synthesized and applied in the reaction of CO_(2)hydrogenation to CO.Among the three nitride catalysts,theγ-Mo_(2)N exhibits superior activity to target product CO,which is 4.6 and 76 times higher than the other two counterparts ofβ-W_(2)N andδ-NbN at 600℃,respectively.Additionally,γ-Mo_(2)N exhibits excellent stability on both cyclic heating-cooling and high space velocity steady state operation.The deactivation degree of cyclic heating-cooling evaluation after 5 cycles and long-term stability performance at 773 and 873 K in 50 h are all less than 10%.In-situ XRD and kinetic studies suggest that theγ-Mo_(2)N itself is able to activate both of the reactants CO_(2)and H_(2).Below 400℃,the reaction mainly occurs at the surface ofγ-Mo_(2)N catalyst.CO_(2)and H_(2)competitively adsorbe on the surface of catalyst and CO_(2)is the relatively stronger surface adsorbate.At a higher temperature,the interstitial vacancies of theγ-Mo_(2)N can be reversibly filled with the oxygen from CO_(2)dissociation.Both of the surface and bulk phase sites ofγ-Mo_(2)N participate in the high temperature CO_(2)hydrogenation pathway.
基金This work was supported by grant(2017YFC1601400)the Ministry of Science and Technology of the People’s Republic of China,Key Project in Science and Technology of Henan Province(182102410083).
文摘Human health is threatened by foodborne illness and Staphylococcus aureus is a common foodborne pathogenic bacteria.It can cause food poisoning when we are infected,therefore,it is necessary to detect pathogenic bacteria.The virulence genes and detection methods of S.aureus are summarized from literatures.Traditional detection methods are simple but need bacteria enrichment and to prolong detection time.Immunological technology has high specificity,but false positive results are easy to occur in its detection.In recent years,molecular biology methods have developed rapidly,and various PCR techniques have been applied to detect foodborne pathogens.Real-time fluorescent quantitative PCR is more effective than ordinary PCR,while price is expensive when use it,the scope of use is limited.LAMP is a flexible detection method,biosensor and flow cytometry are fast but the cost of detection is high and the price is relatively expensive.
基金Supported by Guangxi Science and Technology Program Project of China(GK AD19245169)Yulin City Scientific Research and Technology Development Plan Project(YSK 20204026,2019Cxpt00A4,YSK 20202001,YSK 20204038).
文摘Based on the analysis of the current situation and existing problems of traditional Chinese soft shelled turtle(Trionyx sinensis)breeding industry,combined with the fact that selenium enriched functional agriculture is the future demand of mankind,this paper put forward the necessity of developing selenium enriched Chinese soft shelled turtles and promoting the sustainable development of modern agriculture:the development of selenium-enriched Chinese soft-shelled turtle is the need of the consumer market for food safety,the need to reduce the production cost of soft-shelled turtles and improve the production efficiency of soft-shelled turtles,the need to enrich the Chinese soft-shelled turtle market,and the need to promote the sustainable development of modern agriculture(fishery).
基金supported by the National Key R&D Program of China(Nos.2020YFC2005502 and 2018YFB 1004700)the National Natural Science Foundation of China(Nos,6187238 and 61972254)+2 种基金the Science and Technology Commission of Shanghai Municipality No.19401900500)the Innovation Program of Shanghai Health Commission(Nos.201840121 and ZY(2018-2020)-ZWB-1001-CPJS1)the CCF-Huawei Database System Innovation Research Plan(No.CCF-Huawei DBIR2019002A)and the program of Hospital Clinical Research,Xinhua Hospital Afiliated to Shanghai Jiao Tong University School of Medicine(No.19XHCR11C).
文摘The fracture risk of patients with diabetes is higher than those of patients without diabetes due to hyperglycemia,usage of diabetes drugs,changes in insulin levels,and excretion,and this risk begins as early as adolescence.Many factors including demographic data(such as age,height,weight,and gender),medical history(such as smoking,drinking,and menopause),and examination(such as bone mineral density,blood routine,and urine routine)may be related to bone metabolism in patients with diabetes.However,most of the existing methods are qualitative assessments and do not consider the interactions of the physiological factors of humans.In addition,the fracture risk of patients with diabetes and osteoporosis has not been further studied previously.In this paper,a hybrid model combining XGBoost with deep neural network is used to predict the fracture risk of patients with diabetes and osteoporosis,and investigate the effect of patients’physiological factors on fracture risk.A total of 147 raw input features are considered in our model.The presented model is compared with several benchmarks based on various metrics to prove its effectiveness.Moreover,the top 18 influencing factors of fracture risks of patients with diabetes are determined.
文摘Perovskite solar cells(PSCs)are undergoing rapid development and the power conversion efficiency reaches 25.7%which attracts increasing attention on their commercialization recently.In this review,we summarized the recent progress of PSCs based on device structures,perovskite-based tandem cells,large-area modules,stability,applications and industrialization.Last,the challenges and perspectives are discussed,aiming at providing a thrust for the commercialization of PSCs in the near future.
文摘Exploration of heavy metals and organic pollutants, their leaching capacity along with health and environmental risks in contaminated industrial construction and demolition waste （ICDW） within a pesticide manufacturing plant were investigated. A maximum content of 90.8 mg·kg-1 Cd was found present in the wastes, which might originate from phosphorus rocks and industrial sulfuric acid used in pesticide production processes. An average concentration of 979.8 mg·kg-1 dichlorovos and other 11 organophosphorus pesticide were also detected. Relatively high leaching rates of around 4.14‰ were obtained from laboratory simulated ICDW using both glacial acetic acidsodium hydroxide and deionized water. Pesticide pollutants had the strongest tendency to retaining on dry bricks （leaching rate 1.68 9‰） compared to mortar-coatings, etc. due to their different physical characteristics and octanol-water partioning coefficient. Mobility of pesticide from on-site ICDW by water was spatially correlated to waste types, process sections and human activities, with a flux of leaching rate between 5.9‰ to 27.4%. Risk-based corrective action （RBCA） model was used to simulate the risk of contaminated ICDW debris randomly scattered. Oral and demlal ingestion amount by local workers was 9.8 × 10-3 and 1.9 × 10-2 mg.（kg-d）-1, respectively. Potential leaching risk to aquatic systems exceeded the limit for nearly 75% waste. Environmental and health risk exceedance was found in most ICDW, while the risk value of the most severely contaminated brick waste was 660 times beyond critical level. Implications for waste management involving construction and deconstruction work, waste transferring and regulation supplying were also provided.
基金funding provided by the China Scholarship Council (Nos.202008440524 and 202006370006)supported by the Distinguished Youth Science Foundation of Hunan Province of China (No.2022JJ10073)+1 种基金Innovation Driven Project of Central South University (No.2020CX040)Shenzhen Sciencee and Technology Plan (No.JCYJ20190808123013260).
文摘Concrete is the most commonly used construction material.However,its production leads to high carbon dioxide(CO_(2))emissions and energy consumption.Therefore,developing waste-substitutable concrete components is necessary.Improving the sustainability and greenness of concrete is the focus of this research.In this regard,899 data points were collected from existing studies where cement,slag,fly ash,superplasticizer,coarse aggregate,and fine aggregate were considered potential influential factors.The complex relationship between influential factors and concrete compressive strength makes the prediction and estimation of compressive strength difficult.Instead of the traditional compressive strength test,this study combines five novel metaheuristic algorithms with extreme gradient boosting(XGB)to predict the compressive strength of green concrete based on fly ash and blast furnace slag.The intelligent prediction models were assessed using the root mean square error(RMSE),coefficient of determination(R^(2)),mean absolute error(MAE),and variance accounted for(VAF).The results indicated that the squirrel search algorithm-extreme gradient boosting(SSA-XGB)yielded the best overall prediction performance with R^(2) values of 0.9930 and 0.9576,VAF values of 99.30 and 95.79,MAE values of 0.52 and 2.50,RMSE of 1.34 and 3.31 for the training and testing sets,respectively.The remaining five prediction methods yield promising results.Therefore,the developed hybrid XGB model can be introduced as an accurate and fast technique for the performance prediction of green concrete.Finally,the developed SSA-XGB considered the effects of all the input factors on the compressive strength.The ability of the model to predict the performance of concrete with unknown proportions can play a significant role in accelerating the development and application of sustainable concrete and furthering a sustainable economy.
