Bacterial strain PYX-6 that utilizes anthracene, phenanthrene, or pyrene for carbon and energy sources for growth was isolated from a non-polluted lake (Tianchi Lake) in Xinjiang Uygur Autonomons Region of China. Itsm...Bacterial strain PYX-6 that utilizes anthracene, phenanthrene, or pyrene for carbon and energy sources for growth was isolated from a non-polluted lake (Tianchi Lake) in Xinjiang Uygur Autonomons Region of China. Itsmorphology, physiological and biochemical properties, cellwall pattern and G+C mol% content of DNA molecules were characterized. The 16S rRNA gene of strain PYX-6 wassequenced and analyzed for similarities to related bacterial species. Results indicated that strain PYX-6 is a member of the Genus Saccharothrix, and the strain was namedSaccharothrix sp. PYX-6. When pyrene was the sole carbon source in cultural medium, the strain PYX-6 assimilatedpyrene for growth and 0.005% of yeast extract stimulatedpyrene degradation and assimilation. The optimal pH ofcultural medium and the optimal shaking frequency during cultivation were 6—8 and 200 r/m, respectively. It was found that the disappearance of pyrene in medium occurred before significant growth of strain PYX-6 took place. Phthalic acid, benzylacetic acid, and benzylpropenoic acid were detected as catabolic intermediates during pyrene degradation with mass spectroscopy and this result indicated that Saccharothrix sp. PYX-6 adopted a pathway that is different from the pathway of the previously reported pyrene-degrading Mycobacteriumsp. PYR-1.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.2017734)the Knowledge Innovation Program(Grant No.KSCX2-SW-113,KZCX1-SW-12-Ⅱ-02)of CAS
文摘Bacterial strain PYX-6 that utilizes anthracene, phenanthrene, or pyrene for carbon and energy sources for growth was isolated from a non-polluted lake (Tianchi Lake) in Xinjiang Uygur Autonomons Region of China. Itsmorphology, physiological and biochemical properties, cellwall pattern and G+C mol% content of DNA molecules were characterized. The 16S rRNA gene of strain PYX-6 wassequenced and analyzed for similarities to related bacterial species. Results indicated that strain PYX-6 is a member of the Genus Saccharothrix, and the strain was namedSaccharothrix sp. PYX-6. When pyrene was the sole carbon source in cultural medium, the strain PYX-6 assimilatedpyrene for growth and 0.005% of yeast extract stimulatedpyrene degradation and assimilation. The optimal pH ofcultural medium and the optimal shaking frequency during cultivation were 6—8 and 200 r/m, respectively. It was found that the disappearance of pyrene in medium occurred before significant growth of strain PYX-6 took place. Phthalic acid, benzylacetic acid, and benzylpropenoic acid were detected as catabolic intermediates during pyrene degradation with mass spectroscopy and this result indicated that Saccharothrix sp. PYX-6 adopted a pathway that is different from the pathway of the previously reported pyrene-degrading Mycobacteriumsp. PYR-1.
