Pectin contributes greatly to cell wall phosphorus(P)remobilization.However,it is currently unclear whether the methylesterification degree of the pectin,which is related to the activity of pectin methylesterases(PMEs...Pectin contributes greatly to cell wall phosphorus(P)remobilization.However,it is currently unclear whether the methylesterification degree of the pectin,which is related to the activity of pectin methylesterases(PMEs),is also involved in this process.Here,we demonstrated that elevated PME activity can facilitate the remobilization of P deposited in the cell wall.P-deficient conditions resulted in the reduction of root cell wall P content.This reduction was more pronounced in Nipponbare than in Kasalath,in company with a significant increment of the PME activity,indicating a possible relationship between elevated PME activity and cell wall P remobilization.This hypothesis was supported by in vitro experiments,as pectin with lower methylesterification degree had higher ability to release inorganic P(Pi)from insoluble FePO_(4).Furthermore,among the 35 OsPME members in rice,only the expression of OsPME14 showed a relationship with PME activity.In addition,transgenic rice lines overexpressing OsPME14 had increased PME activity,released more P from the root cell wall,and more resistant to P deficiency.In conclusion,PMEs enhance P remobilization in P-starved rice by increasing PME activity in Nipponbare,which in turn helps to remobilize P from the cell wall,and thus makes more available P.展开更多
Objective:To observe the clinical efficacy of row needling at the Governor Vessel on the head for poststroke insomnia,analyze the changes of sleep structure,serum amino acid neurotransmitters,vascular endothelial cell...Objective:To observe the clinical efficacy of row needling at the Governor Vessel on the head for poststroke insomnia,analyze the changes of sleep structure,serum amino acid neurotransmitters,vascular endothelial cell function and cognitive function,in order to explore the mechanism of this therapy.Methods:A total of 41 patients with poststroke insomnia were included in an observation group and treated with row needing at the Governor Vessel on the head;33 patients with poststroke insomnia were included in a control group and given routine acupuncture treatment.The patients in both groups were treated consecutively for one month.The efficacy was observed and the changes of related indicators were compared.Results:After treatment,the efficacy of the observation group was better than that of the control group(P<0.05).The sleep latency in the observation group was shorter than that in the control group(P<0.05);the total sleep duration,deep sleep duration,light sleep duration and rapid eye movement sleep duration in the observation group were significantly longer than those in the control group(P<0.05);the total waking duration and the waking times over 5 min were significantly less than those in the control group(P<0.05).The serum glutamic acid,γ-aminobutyric acid,calcitonin gene-related peptide,brain-derived neurotrophic factor,nerve growth factor and nitric oxide levels were significantly higher than those in the control group(P<0.01).The tumor necrosis factor-α,interleukin-6,bradykinin,and endothelin-1 levels were lower than those in the control group(P<0.01).The Pittsburgh sleep quality index score in the observation group was lower than that in the control group(P<0.01),and the mini-mental state examination score was higher than that in the control group(P<0.05).Conclusion:Row needling at the Governor Vessel on the head can effectively improve the sleep structure,cognitive function,and sleep quality of patients with poststroke insomnia.The possible mechanisms are regulating serum amino acid neurotransmitters and improving the function of vascular endothelial cells.展开更多
基金This study was supported by the Foundation for Distinguished Young Scholars of Jiangsu Province,China(Grant No.BK20190050)Youth Innovation Promotion Association of Chinese Academy of Sciences(Grant No.2015250)+2 种基金the‘Strategic Priority Research Program’of the Chinese Academy of Sciences(Grant No.XDB15030300)National Science Foundation of China(Grant No.31501825)the State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources in China in 2020(Grant No.SKLCUSA-b201909).
文摘Pectin contributes greatly to cell wall phosphorus(P)remobilization.However,it is currently unclear whether the methylesterification degree of the pectin,which is related to the activity of pectin methylesterases(PMEs),is also involved in this process.Here,we demonstrated that elevated PME activity can facilitate the remobilization of P deposited in the cell wall.P-deficient conditions resulted in the reduction of root cell wall P content.This reduction was more pronounced in Nipponbare than in Kasalath,in company with a significant increment of the PME activity,indicating a possible relationship between elevated PME activity and cell wall P remobilization.This hypothesis was supported by in vitro experiments,as pectin with lower methylesterification degree had higher ability to release inorganic P(Pi)from insoluble FePO_(4).Furthermore,among the 35 OsPME members in rice,only the expression of OsPME14 showed a relationship with PME activity.In addition,transgenic rice lines overexpressing OsPME14 had increased PME activity,released more P from the root cell wall,and more resistant to P deficiency.In conclusion,PMEs enhance P remobilization in P-starved rice by increasing PME activity in Nipponbare,which in turn helps to remobilize P from the cell wall,and thus makes more available P.
文摘Objective:To observe the clinical efficacy of row needling at the Governor Vessel on the head for poststroke insomnia,analyze the changes of sleep structure,serum amino acid neurotransmitters,vascular endothelial cell function and cognitive function,in order to explore the mechanism of this therapy.Methods:A total of 41 patients with poststroke insomnia were included in an observation group and treated with row needing at the Governor Vessel on the head;33 patients with poststroke insomnia were included in a control group and given routine acupuncture treatment.The patients in both groups were treated consecutively for one month.The efficacy was observed and the changes of related indicators were compared.Results:After treatment,the efficacy of the observation group was better than that of the control group(P<0.05).The sleep latency in the observation group was shorter than that in the control group(P<0.05);the total sleep duration,deep sleep duration,light sleep duration and rapid eye movement sleep duration in the observation group were significantly longer than those in the control group(P<0.05);the total waking duration and the waking times over 5 min were significantly less than those in the control group(P<0.05).The serum glutamic acid,γ-aminobutyric acid,calcitonin gene-related peptide,brain-derived neurotrophic factor,nerve growth factor and nitric oxide levels were significantly higher than those in the control group(P<0.01).The tumor necrosis factor-α,interleukin-6,bradykinin,and endothelin-1 levels were lower than those in the control group(P<0.01).The Pittsburgh sleep quality index score in the observation group was lower than that in the control group(P<0.01),and the mini-mental state examination score was higher than that in the control group(P<0.05).Conclusion:Row needling at the Governor Vessel on the head can effectively improve the sleep structure,cognitive function,and sleep quality of patients with poststroke insomnia.The possible mechanisms are regulating serum amino acid neurotransmitters and improving the function of vascular endothelial cells.
