Low temperature(LT)in spring has become one of the principal abiotic stresses that restrict the growth and development of wheat.Diverse analyses were performed to investigate the mechanism underlying the response of w...Low temperature(LT)in spring has become one of the principal abiotic stresses that restrict the growth and development of wheat.Diverse analyses were performed to investigate the mechanism underlying the response of wheat grain development to LT stress during booting.These included morphological observation,measurements of starch synthase activity,and determination of amylose and amylopectin content of wheat grain after exposure to treatment with LT during booting.Additionally,proteomic analysis was performed using tandem mass tags(TMT).Results showed that the plumpness of wheat grains decreased after LT stress.Moreover,the activities of sucrose synthase(SuS,EC 2.4.1.13)and ADP-glucose pyrophosphorylase(AGPase,EC 2.7.7.27)exhibited a significant reduction,leading to a significant reduction in the contents of amylose and amylopectin.A total of 509 differentially expressed proteins(DEPs)were identified by proteomics analysis.The Gene Ontology(GO)enrichment analysis showed that the protein difference multiple in the nutritional repository activity was the largest among the molecular functions,and the up-regulated seed storage protein(ssP)played an active role in the response of grains to LT stress and subsequent damage.The Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis showed that LT stress reduced the expression of DEPs such as sucrose phosphate synthase(SPS),glucose-1-phosphate adenylyltransferase(glgC),andβ-fructofuranosidase(FFase)in sucrose and starch metabolic pathways,thus affecting the synthesis of grain starch.In addition,many heat shock proteins(HsPs)were found in the protein processing in endoplasmic reticulum pathways,which can resist some damage caused by LT stress.These findings provide a new theoretical foundation for elucidating the underlying mechanism governing wheat yield developmentafterexposuretoLTstress inspring.展开更多
Ti-10V-2Fe-3Al alloy with fine-grainedβphases was fabricated by friction stir processing with opti-mized processing parameters.The superplastic behavior of the specimens was investigated by tensile deformation at dif...Ti-10V-2Fe-3Al alloy with fine-grainedβphases was fabricated by friction stir processing with opti-mized processing parameters.The superplastic behavior of the specimens was investigated by tensile deformation at different strain rates and temperatures,and an optimal superplastic elongation of 634%was achieved at 700℃ and 3×10^(-4)/s.An annealing treatment at 650℃ for 60 min showed a mi-crostructure withαprecipitates distributed in theβmatrix in the friction stir specimen.Such pre-heat treatment improves the superplasticity of the specimen,achieving an elongation of up to 807%at 750℃ and 3×10^(-4)/s.The influences of tensile temperatures and strain rates on the microstructural evolution,such as grain size variation,grain morphology,and phase transformations,were discussed.The super-plastic deformation behavior of fine-grained Ti-10V-2Fe-3Al alloy is controlled by grain boundary sliding and accompanied by dynamic phase transformation and recrystallization.展开更多
Purines are building blocks for DNA and RNA,found as the energy currency of cells(ATP and GTP),used as signaling molecules(cGMP,cAMP and ATP),and served as precursors for synthesizing primary products such as polysacc...Purines are building blocks for DNA and RNA,found as the energy currency of cells(ATP and GTP),used as signaling molecules(cGMP,cAMP and ATP),and served as precursors for synthesizing primary products such as polysaccharides,sucrose,and phospholipids as well as secondary products(Stasolla et al.,2003;Pareek et al.,2020).Thus,the synthesis of purines is a critical pathway in the cells of all living organisms.Purines can be synthesized through two pathways:de novo and salvage(Stasolla et al.,2003).Recent investigations revealed that purine synthesis is vital for the proper development of chloroplasts in plants.In Arabidopsis,CIA1 encodes the enzyme glutamine phosphoribosyl pyrophosphate amidotransferase,which catalyzes the first committed step of purine de novo biosynthesis,the loss-of-function mutant cia1 shows small,pale-green mosaic leaves(Hung et al.,2004).In rice,both VAL1 and GARS encode glycinamide ribonucleotide synthetase that mediates the second step in purine biosynthesis.展开更多
Geniposide,the principal active iridoid glucoside ingredient in Fructus gardeniae used in numerous traditional Chinese clinical prescriptions,has been shown to cause herbal hepatotoxicity because of its glycone metabo...Geniposide,the principal active iridoid glucoside ingredient in Fructus gardeniae used in numerous traditional Chinese clinical prescriptions,has been shown to cause herbal hepatotoxicity because of its glycone metabolite genipin.This study explored the role of gut microbiota in alleviating geniposide hepatotoxicity with isoflavones in soy products.Metabolic profiling using ultra high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UHPLC-Q/TOF-MS)revealed two metabolic pathways and six main forms of geniposides in vivo.Enzyme inhibitor experiments have shown that isoflavones alter geniposide metabolism by mediating specific enzymes,includingβ-glucosidase(β-GC)and sulfotransferase(SULT),in an established pseudo-sterile rat model.Isoflavones pretreatment by gavage for three weeks optimized the structure of the gut microbiota was linked to the regulation of key metabolic enzymes.Furthermore,experiments involving fecal microbiota transplantation(FMT)established the direct contribution of the gut microbiota to the regulation of enzyme activities and geniposide metabolism.This study demonstrated that isoflavones in soy products regulated the metabolic enzymes of geniposode dependent on gut microbiota,especially Lactobacillus spp.,which was further verified in our clinical trials analyzed using 16S ribosomal RNA(rRNA)and metagenomic sequencing,thus regulating geniposide metabolism.Furthermore,as dominant beneficial bacterium,Lactobacillus spp.were discovered to be promising microbial targets for the better management of geniposide hepatotoxicity.These findings provide valuable insights for the prevention and intervention of drug-induced liver injury.展开更多
Ogura cytoplasmic male sterility(Ogura CMS)was first identified in wild radish(Raphanus sativus)and resulted in complete pollen abortion.However,the molecular mechanism of Ogura CMS in Chinese cabbage remains unclear....Ogura cytoplasmic male sterility(Ogura CMS)was first identified in wild radish(Raphanus sativus)and resulted in complete pollen abortion.However,the molecular mechanism of Ogura CMS in Chinese cabbage remains unclear.A cytological analysis confirmed nuclear degradation during the late uninucleate stage of pollen development,which diminished by the tricellular stage.Concurrently,tapetal cells exhibited abnormal enlargement and vacuolation starting from the tetrad stage.Serious developmental defects were observed in the pollen wall.During early pollen development,genes associated with cytochrome c and programmed cell death(PCD)were upregulated in the Ogura CMS line,while genes involved in pollen wall mitosis were downregulated.Conversely,at the late stage of pollen development,peroxisome and autophagy-related genes in the Ogura CMS line were upregulated.The mitochondrial orf138 gene mutation triggered the PCD process in tapetal cells,leading to their abnormal enlargement and the degradation of their contents,eventually resulting in vacuolation at the tricellular stage.These tapetal defects hindered the provision of adequate sporopollenin and nutrients to the microspores,consequently leading to abnormal pollen wall development and abnormal mitosis in the microspores.Ultimately,nuclear dispersion commenced during the late uninucleate stage,and autophagy occurred in the late stage of pollen development.Consequently,the plant could not produce functional pollen,resulting in male sterility in Chinese cabbage.Studies of Ogura CMS can promote the production and application of male sterile materials and enrich male sterile resources,which is of great significance for hybrid breeding.展开更多
Dysregulated inflammatory reactions can result in detrimental effects to the body,thereby causing various diseases.Traditional treatments relying on anti-inflammatory drugs or nanoformulations often undermine the body...Dysregulated inflammatory reactions can result in detrimental effects to the body,thereby causing various diseases.Traditional treatments relying on anti-inflammatory drugs or nanoformulations often undermine the body’s physiological immune functions or potentially exhibit biotoxicity.Extracellular vesicles,which contain a diverse array of antiinflammatory substances and possess nanomedicine transport properties,are emerging as highly promising candidates for next-generation drug delivery systems and active biological agents.Plant-derived vesicle-like nanoparticles(PDVLNs)are nanostructured particles isolated from plants.Given their wide availability and low immunogenicity,PDVLNs are considered to hold great potential in the treatment of inflammatory diseases(IDs).In this review,we introduce the principle,design consideration and treatment mechanism associated with PDVLNs in treating various IDs.Specifically,the natural ingredients carried by PDVLNs not only help eliminate danger signals such as reactive oxygen species and reactive nitrogen species,but also hinder the initiation of inflammatory responses through various mechanisms.Moreover,engineered PDVLNs nanotechnology has been successfully employed in the treatment of IDs.Finally,the review discusses the current opportunities and challenges in this field and provides insights for the future clinical applications of PDVLNs in treating IDs.展开更多
Polycystic ovarian syndrome(PCOS)is a frequently encountered disease with hormonal and metabolic dysfunction in women of reproductive age.Nano-sized titanium dioxide(nTiO_(2))has been widely used in skin care products...Polycystic ovarian syndrome(PCOS)is a frequently encountered disease with hormonal and metabolic dysfunction in women of reproductive age.Nano-sized titanium dioxide(nTiO_(2))has been widely used in skin care products,paints,food,and cosmetics.In this study,we found a substantial elevation of Ti in the follicular fluid of females diagnosed with nonhyperandrogenic PCOS(non-HA PCOS).Therefore,we orally subjected female rats to nTiO_(2) at 10 and 100 mg/kg body weight for 10 weeks.We found that nTiO_(2) triggered non-HA PCOS-like symptoms,including the increase of cystic follicles,estrous cycle disorder,and abnormal ovulation,but without high testosterone.By transcriptome sequencing,we identified that nTiO_(2) exposure inhibited the steroid hormone synthesis and activated the proapoptotic pathways.Upon validating these altered pathways,we discovered that aromatase(Cyp19a1)overexpression contributed to the decline in testosterone/estradiol ratio.Mean-while,the apoptosis of granulosa cells was increased by nTiO_(2) exposure,which might result in the disorder of follicular development.This study presents the evidences revealing a pronounced correlation between nTiO_(2) exposure and the emergence of non-HA PCOS,urging people to promote the safe utilization of nTiO_(2).展开更多
Tumor heterogeneity and diversity significantly undermine the effectiveness of monotherapy.Collaborative therapy emerges as a promising approach to mitigate tumor recurrence resulting from monotherapy.Combining chemod...Tumor heterogeneity and diversity significantly undermine the effectiveness of monotherapy.Collaborative therapy emerges as a promising approach to mitigate tumor recurrence resulting from monotherapy.Combining chemodynamic therapy(CDT)with photothermal therapy(PTT)offers a compelling solution for eradicating residual tumor cells post-PTT.In this study,we harness the Fenton-like response facilitated by glucose oxidase(GOD)and the mild hyperthermia induced by polyethyleneimine(PEI)functionalized nitrogen-containing graphene oxide to enhance tumor therapy through a metal-free bionic nanozyme.GOD catalyzes a substantial amount of hydrogen peroxide,and,with the carrier's involvement,triggers a Fenton-like reaction,yielding a wealth of hydroxyl radicals.These hydroxyl radicals effectively target tumor cells following photothermal action,bolstering CDT and culminating in a bidirectional amplification treatment that effectively prevents tumor recurrence and metastasis.This research amalgamates the physical and chemical attributes of nanomaterials with the unique characteristics of the tumor microenvironment,presenting a compelling and efficacious alternative for tumor treatment.展开更多
Solid-to-solid interfacial issues are one of the most intractable problems hindering the practical application of all-solid-state batteries(ASSBs).The interfacial instability behaviors caused by the rough interface be...Solid-to-solid interfacial issues are one of the most intractable problems hindering the practical application of all-solid-state batteries(ASSBs).The interfacial instability behaviors caused by the rough interface between lithium anode and solid electrolyte(SE)involve complicated electro-chemo-mechanics interplays and their quantitative relationships still remain unclear.The three-dimensional electro-chemomechanical coupled model with randomly generated rough lithium-SE interface is developed in this study to investigate the effects of interface roughness on the interfacial failure behaviors.Results demonstrate that the existence of a rough lithium-SE interface causes the highly concentrated strain,GPa-level stress,and localized current density at the protruding tips,probably inducing dendrite formation and interface cracking.The interface roughness effect is much more pronounced in lithium anode than graphite anode due to their different Li storage mechanisms,i.e.,surface deposition and Li intercalation.Excessive stack pressure(>50 MPa)magnifies the stress effect on overpotential to enlarge the current density localization and deteriorate the interfacial instability issues.Reducing interface roughness through surface treatment,together with regulation of external operation conditions,can effectively improve interfacial stability performance.The results provide an in-depth understanding of the underlying electro-chemo-mechanical coupling mechanism caused by the rough anode-SE interface and bring more insights into further improvement of ASSBs'enhanced reliability and longevity.展开更多
The pore structures of the Majiagou Formation in the Ordos Basin are complex,featuring micro-and nano-scale intra-crystalline and inter-crystalline pores that significantly impact hydrocarbon storage and flow.Precisel...The pore structures of the Majiagou Formation in the Ordos Basin are complex,featuring micro-and nano-scale intra-crystalline and inter-crystalline pores that significantly impact hydrocarbon storage and flow.Precisely characterizing the rock internal structures is crucial for reservoir exploration and development.However,it is difficult to accurately characterize the pore structure of rock using traditional imaging methods to meet the simulation requirements.In this context,this study focuses on high-resolution 3D digital core reconstruction using the SliceGAN model.Specifically,the Modular Automated Processing System(MAPS)image and Quanti-tative Evaluation of Minerals by Scanning Electron Microscopy(QEMSCAN)image were combined to divide MAPS into three categories:pore,dolomite,and calcite.Then,through the SliceGAN algorithm,the 3D digital core was reconstructed.To evaluate the reconstruction,the auto-correlation function,two-point probability function,porosity,mineral content,and specific surface area were employed.The results show that the SliceGAN can effectively capture the micro-features in the core,and the internal structure of the generated core was consistent with that of the original core.This study provided a new sight for reconstructing cores with complex pore structures and strong heterogeneity and innovatively supports tight carbonate reservoir characterization and evaluation.展开更多
Improving the surface atoms utilization efficiency of catalysts is extremely important for large-scale H_(2)production by electrochemical water splitting,but it remains a great challenge.Herein,we reported two kinds o...Improving the surface atoms utilization efficiency of catalysts is extremely important for large-scale H_(2)production by electrochemical water splitting,but it remains a great challenge.Herein,we reported two kinds of Mo O_(3)-polyoxometalate hybrid nanobelt superstructures(MoO_(3)-POM HNSs,POM=PW_(12)O_(40)and Si W_(12)O_(40))using a simple hydrothermal method.Such superstructure with highly uniform nanoparticles as building blocks can expose more surface atoms and emanate increased specific surface area.The incorporated POMs generated abundant oxygen vacancies,improved the electronic mobility,and modulated the surface electronic structure of MoO_(3),allowing to optimize the H^(*)adsorption/desorption and dehydrogenation kinetics of catalyst.Notably,the as-prepared MoO_(3)-PW_(12)O_(40)HNSs electrodes not only displayed the low overpotentials of 108 mV at 10 mA/cm^(2)current density in 0.5 mol/L H_(2)SO_(4)electrolyte but also displayed excellent long-term stability.The hydrogen evolution reaction(HER)performance of MoO_(3)-POM superstructures is significantly better than that of corresponding bulk materials MoO_(3)@PW_(12)O_(40)and Mo O_(3)@Si W_(12)O_(40),and the overpotentials are about 8.3 and 4.9 times lower than that of single Mo O_(3).This work opens an avenue for designing highly surface-exposed catalysts for electrocatalytic H_(2)production and other electrochemical applications.展开更多
Partial phase transformation in NiTi-based refrigerants usually enables efficient and durable elastocaloric cooling,but its thermomechanical behavior with varying temperatures remains unclear.Keeping this in view,the ...Partial phase transformation in NiTi-based refrigerants usually enables efficient and durable elastocaloric cooling,but its thermomechanical behavior with varying temperatures remains unclear.Keeping this in view,the elastocaloric effect of NiTi under incomplete transformation across 15-100℃ is investigated and a superelastic deformation window between 25 and 85℃ is identified.Synchronous infrared thermography and digital image correlation,and an innovative macro-micro phase-field model are employed to examine martensitic transformation and elastocaloric properties of NiTi within the superelastic window.Experimental and simulated results consistently reveal that the spatiotemporal thermal profiles correlate with Lüders strain band evolution.As superelastic deformation temperature increases,strain localization intensifies,with Lüders bands favoring an inward strain growth over an outward expansion,resulting in a smaller yet more deformed martensitic transformation zone.The aggravated strain inhomogeneity makes the local endothermic undercooling tested at 85℃ up to about twice(−30.05℃)that at 25℃(−15.32℃),boosting the global cooling capacity by 65%,despite constant strain.The seeming contradiction between the larger elastocaloric effect and the narrower apparent martensitic transformation zone is elucidated by recourse to the simulations.It is found that the martensitic transformation within the Lüders bands is incomplete,proceeding in a macroscopically uniform but microscopically heterogeneous manner.Elevated temperatures within the superelastic window increase the transformed volume fraction and enhance martensitic transformation,thereby strengthening the global caloric effect.The work sheds light on the interplay between partial martensitic transformation and thermal behavior in NiTi under varying superelastic deformation temperatures,providing insights for advanced elastocaloric cooling applications.展开更多
Background:Viral myocarditis(VMC)is an inflammatory myocardial disease induced by viral infections,and currently,there are no effective targeted treatments available.Cyclovirobuxine D(CVB-D),a major alkaloid extracted...Background:Viral myocarditis(VMC)is an inflammatory myocardial disease induced by viral infections,and currently,there are no effective targeted treatments available.Cyclovirobuxine D(CVB-D),a major alkaloid extracted from the traditional eastern medicinal plant Cephalotaxus,has been clinically used in the treatment of arrhythmias and coronary heart disease.However,its therapeutic effects and underlying mechanisms in VMC remain unclear.This study aims to investigate the cardioprotective effects of CVB-D in a murine model of viral myocarditis induced by Coxsackievirus B3(CVB3)and to elucidate the molecular mechanisms involved in its effects.Method:A murine model of VMC was established by infecting C57BL/6J mice with CVB3.The mice were randomly assigned to three groups:the sham-operated group,the model group,and the CVB-D treatment group.After 14 days of intervention,the general health status of the mice was evaluated.Histopathological changes in myocardial tissue and myocardial cell cross-sectional areas were examined using Hematoxylin and Eosin(H&E)staining.Cardiac function was assessed via echocardiography.Additionally,network pharmacology was employed to identify VMC-related targets and signaling pathways,which were further analyzed to hypothesize the potential therapeutic mechanisms of CVB-D in VMC.Result:CVB-D exerts significant protective effects against CVB3-induced VMC.The underlying mechanisms may be associated with mitochondrial energy metabolism,the electron transport chain,and oxidative phosphorylation processes.This study provides experimental evidence supporting the potential of CVB-D as a therapeutic agent for VMC.展开更多
Coral reefs support a wide range of organisms in the world,including jellyfish and their benthic relatives.However,quantifying the biodiversity of these organisms in reefs is a challenge because of their uneven distri...Coral reefs support a wide range of organisms in the world,including jellyfish and their benthic relatives.However,quantifying the biodiversity of these organisms in reefs is a challenge because of their uneven distribution and cryptic early life stages,requiring the validation of alternative techniques for biodiversity assessment.Here,the biodiversity and spatial distribution patterns of jellyfish and their benthic relatives,from the Scyphozoa,Hydrozoa,and Ctenophora taxa(hereafter referred to as SHC),were investigated in the coral reefs of Xisha,China,using environmental DNA(eDNA)metabarcoding technology by collecting shallow seawater,mesophotic seawater,and sediment samples.One-hundred and eighty-eight SHC species spanning two phyla(Cnidaria and Ctenophora),three classes,11 orders,65 families,and 104 genera were identified,among which hydrozoans were the most dominant taxa,accounting for 89.81% of all SHC species.SHC species showed low connectivity between shallow and mesophotic habitats,presenting a clear vertical distribution pattern in coral reefs.In the mesophotic coral ecosystems(MCEs),140 SHC species(84.34%)were detected,of which 39.76% were exclusive to MCEs,with Zanclea sp.1,Orthopyxis integra,and Fabienna sphaerica being the dominant species.Additionally,although SHC diversity in seawater was higher than that in the sediment samples,22 species were identified only in the sediment samples,indicating that sediment eDNA may represent a valuable supplementary tool for the investigation of SHC communities in hot spots.In addition to revealing the vast diversity of SHC species occupying coral reef ecosystems in the Xisha Islands,our findings confirm the potential of eDNA metabarcoding as an advanced tool for monitoring the biodiversity of cryptic species.展开更多
Global warming is primarily characterized by asymmetric temperature increases,with greater temperature rises in winter/spring and at night compared to summer/autumn and the daytime.We investigated the impact of winter...Global warming is primarily characterized by asymmetric temperature increases,with greater temperature rises in winter/spring and at night compared to summer/autumn and the daytime.We investigated the impact of winter night warming on the top expanded leaves of the spring wheat cultivar Yangmai 18 and the semi-winter wheat cultivar Yannong 19 during the 2020-2021 growing season.Results showed that the night-time mean temperature in the treatment group was 1.27°C higher than the ambient temperature,and winter night warming increased the yields of both wheat cultivars,the activities of sucrose synthase and sucrose phosphate synthase after anthesis,and the biosynthesis of sucrose and soluble sugars.Differentially expressed genes(DEGs)were identified using criteria of P-value<0.05 and fold change>2,and they were subjected to Gene Ontology(GO)annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses.Genes differentially expressed in wheat leaves treated with night warming were primarily associated with starch and sucrose metabolism,amino acid biosynthesis,carbon metabolism,plant hormone signal transduction,and amino sugar and nucleotide sugar metabolism.Comparisons between the groups identified 14 DEGs related to temperature.These results highlight the effects of winter night warming on wheat development from various perspectives.Our results provide new insights into the molecular mechanisms of the response of wheat to winter night warming and the candidate genes involved in this process.展开更多
Language learning and cultural communication are inherently intertwined and mutually reinforcing.In the context of college English instruction,emphasizing“telling China’s stories well”aligns with the principles of ...Language learning and cultural communication are inherently intertwined and mutually reinforcing.In the context of college English instruction,emphasizing“telling China’s stories well”aligns with the principles of foreign language education and inevitably promotes cultural exchange and mutual understanding across different cultures through foreign language learning.Many researchers have explored ways of cultivating students’ability to communicate Chinese stories and spread Chinese culture in English.However,a few studies have been performed on exploring paths of incorporating regional culture into English language education.In particular,incorporating Qinghai’s indigenous cultural resources into college English teaching practice is rare.Therefore,a survey was conducted to ascertain the present situation concerning integrating Qinghai’s regional culture into college English teaching practice at Qinghai University.Based on the data from the survey,the present study discusses the results and implications obtained during the present teaching process and probes into practical strategies for the infiltration of Qinghai’s regional culture into college English teaching from the dimensions of curriculum,teaching materials,approaches,teachers’professional development,and teaching evaluation,intending to achieve the goals of language teaching and cultural education in the college English course.展开更多
基金supported by the National Natural Science Foundation of China(32372223)the National Key Research and Development Program of China(2022YFD2301404)+1 种基金the College Students'Innovationand Entrepreneurship Training Program of Anhui Province,China(S202210364136)the Natural Science Research Project of Anhui Educational Committee,China(2023AH040133).
文摘Low temperature(LT)in spring has become one of the principal abiotic stresses that restrict the growth and development of wheat.Diverse analyses were performed to investigate the mechanism underlying the response of wheat grain development to LT stress during booting.These included morphological observation,measurements of starch synthase activity,and determination of amylose and amylopectin content of wheat grain after exposure to treatment with LT during booting.Additionally,proteomic analysis was performed using tandem mass tags(TMT).Results showed that the plumpness of wheat grains decreased after LT stress.Moreover,the activities of sucrose synthase(SuS,EC 2.4.1.13)and ADP-glucose pyrophosphorylase(AGPase,EC 2.7.7.27)exhibited a significant reduction,leading to a significant reduction in the contents of amylose and amylopectin.A total of 509 differentially expressed proteins(DEPs)were identified by proteomics analysis.The Gene Ontology(GO)enrichment analysis showed that the protein difference multiple in the nutritional repository activity was the largest among the molecular functions,and the up-regulated seed storage protein(ssP)played an active role in the response of grains to LT stress and subsequent damage.The Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis showed that LT stress reduced the expression of DEPs such as sucrose phosphate synthase(SPS),glucose-1-phosphate adenylyltransferase(glgC),andβ-fructofuranosidase(FFase)in sucrose and starch metabolic pathways,thus affecting the synthesis of grain starch.In addition,many heat shock proteins(HsPs)were found in the protein processing in endoplasmic reticulum pathways,which can resist some damage caused by LT stress.These findings provide a new theoretical foundation for elucidating the underlying mechanism governing wheat yield developmentafterexposuretoLTstress inspring.
基金financially supported by the National Natural Science Foundation of China(No.52105373)the China Scholarship Council(No.202106020094).
文摘Ti-10V-2Fe-3Al alloy with fine-grainedβphases was fabricated by friction stir processing with opti-mized processing parameters.The superplastic behavior of the specimens was investigated by tensile deformation at different strain rates and temperatures,and an optimal superplastic elongation of 634%was achieved at 700℃ and 3×10^(-4)/s.An annealing treatment at 650℃ for 60 min showed a mi-crostructure withαprecipitates distributed in theβmatrix in the friction stir specimen.Such pre-heat treatment improves the superplasticity of the specimen,achieving an elongation of up to 807%at 750℃ and 3×10^(-4)/s.The influences of tensile temperatures and strain rates on the microstructural evolution,such as grain size variation,grain morphology,and phase transformations,were discussed.The super-plastic deformation behavior of fine-grained Ti-10V-2Fe-3Al alloy is controlled by grain boundary sliding and accompanied by dynamic phase transformation and recrystallization.
基金funded by the National Natural Science Foundation of China(Grant No.32202485)the Fund for Distinguished Young Scholars from Henan Academy of Agricultural Sciences(Grant No.2024JQ02)+1 种基金the Zhongyuan Sci-Tech Innovation Leading Talents(Grant No.244200510041)the Key SciTech R&D Project of Joint Foundation in Henan Province(Grant No.232301420024).
文摘Purines are building blocks for DNA and RNA,found as the energy currency of cells(ATP and GTP),used as signaling molecules(cGMP,cAMP and ATP),and served as precursors for synthesizing primary products such as polysaccharides,sucrose,and phospholipids as well as secondary products(Stasolla et al.,2003;Pareek et al.,2020).Thus,the synthesis of purines is a critical pathway in the cells of all living organisms.Purines can be synthesized through two pathways:de novo and salvage(Stasolla et al.,2003).Recent investigations revealed that purine synthesis is vital for the proper development of chloroplasts in plants.In Arabidopsis,CIA1 encodes the enzyme glutamine phosphoribosyl pyrophosphate amidotransferase,which catalyzes the first committed step of purine de novo biosynthesis,the loss-of-function mutant cia1 shows small,pale-green mosaic leaves(Hung et al.,2004).In rice,both VAL1 and GARS encode glycinamide ribonucleotide synthetase that mediates the second step in purine biosynthesis.
基金supported by the National Natural Science Foundation of China(82461160264,82474056,and 82104124)the grant from the Noncommunicable Chronic Diseases-National Science and Technology Major Project(2023ZD0502605)+2 种基金the Science and Technology Development Fund,Macao SAR(FDCT 0025/2021/A1)the Shanghai Municipal Health Commission(2022XD037)the Shanghai Magnolia Talent Plan Pujiang Project(23PJD113)。
文摘Geniposide,the principal active iridoid glucoside ingredient in Fructus gardeniae used in numerous traditional Chinese clinical prescriptions,has been shown to cause herbal hepatotoxicity because of its glycone metabolite genipin.This study explored the role of gut microbiota in alleviating geniposide hepatotoxicity with isoflavones in soy products.Metabolic profiling using ultra high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UHPLC-Q/TOF-MS)revealed two metabolic pathways and six main forms of geniposides in vivo.Enzyme inhibitor experiments have shown that isoflavones alter geniposide metabolism by mediating specific enzymes,includingβ-glucosidase(β-GC)and sulfotransferase(SULT),in an established pseudo-sterile rat model.Isoflavones pretreatment by gavage for three weeks optimized the structure of the gut microbiota was linked to the regulation of key metabolic enzymes.Furthermore,experiments involving fecal microbiota transplantation(FMT)established the direct contribution of the gut microbiota to the regulation of enzyme activities and geniposide metabolism.This study demonstrated that isoflavones in soy products regulated the metabolic enzymes of geniposode dependent on gut microbiota,especially Lactobacillus spp.,which was further verified in our clinical trials analyzed using 16S ribosomal RNA(rRNA)and metagenomic sequencing,thus regulating geniposide metabolism.Furthermore,as dominant beneficial bacterium,Lactobacillus spp.were discovered to be promising microbial targets for the better management of geniposide hepatotoxicity.These findings provide valuable insights for the prevention and intervention of drug-induced liver injury.
基金supported by the China Agricultural Research System(CARS-23-G15)the Innovation Team of Henan Academy of Agricultural Sciences,China(2024TD06)+2 种基金the Autonomous Innovation Project of Henan Academy of Agricultural Sciences,China(2024ZC034)the Joint Research on Agricultural Variety Improvement of Henan Province,China(2022010504)the Key Research&Development Project of Henan Province,China(221111110100)。
文摘Ogura cytoplasmic male sterility(Ogura CMS)was first identified in wild radish(Raphanus sativus)and resulted in complete pollen abortion.However,the molecular mechanism of Ogura CMS in Chinese cabbage remains unclear.A cytological analysis confirmed nuclear degradation during the late uninucleate stage of pollen development,which diminished by the tricellular stage.Concurrently,tapetal cells exhibited abnormal enlargement and vacuolation starting from the tetrad stage.Serious developmental defects were observed in the pollen wall.During early pollen development,genes associated with cytochrome c and programmed cell death(PCD)were upregulated in the Ogura CMS line,while genes involved in pollen wall mitosis were downregulated.Conversely,at the late stage of pollen development,peroxisome and autophagy-related genes in the Ogura CMS line were upregulated.The mitochondrial orf138 gene mutation triggered the PCD process in tapetal cells,leading to their abnormal enlargement and the degradation of their contents,eventually resulting in vacuolation at the tricellular stage.These tapetal defects hindered the provision of adequate sporopollenin and nutrients to the microspores,consequently leading to abnormal pollen wall development and abnormal mitosis in the microspores.Ultimately,nuclear dispersion commenced during the late uninucleate stage,and autophagy occurred in the late stage of pollen development.Consequently,the plant could not produce functional pollen,resulting in male sterility in Chinese cabbage.Studies of Ogura CMS can promote the production and application of male sterile materials and enrich male sterile resources,which is of great significance for hybrid breeding.
基金supported by the National Nature Science Foundation of China[grant numbers 82204939,82104701]the Youth Project of Natural Science Foundation of Anhui Province[grant number 2208085QH268]+3 种基金the Excellent Youth Fund Project of Anhui Provincial Department of Education(grant numbers 2023AH030069)the Key Project of Anhui Province Department of Education[grant numbers 2024AH051051]Anhui University Collaborative Innovation Project[grant number GXXT-2022-061]Anhui University of Chinese Medicine Foundation[grant numbers 2022rcZD001,2022rcZD004]。
文摘Dysregulated inflammatory reactions can result in detrimental effects to the body,thereby causing various diseases.Traditional treatments relying on anti-inflammatory drugs or nanoformulations often undermine the body’s physiological immune functions or potentially exhibit biotoxicity.Extracellular vesicles,which contain a diverse array of antiinflammatory substances and possess nanomedicine transport properties,are emerging as highly promising candidates for next-generation drug delivery systems and active biological agents.Plant-derived vesicle-like nanoparticles(PDVLNs)are nanostructured particles isolated from plants.Given their wide availability and low immunogenicity,PDVLNs are considered to hold great potential in the treatment of inflammatory diseases(IDs).In this review,we introduce the principle,design consideration and treatment mechanism associated with PDVLNs in treating various IDs.Specifically,the natural ingredients carried by PDVLNs not only help eliminate danger signals such as reactive oxygen species and reactive nitrogen species,but also hinder the initiation of inflammatory responses through various mechanisms.Moreover,engineered PDVLNs nanotechnology has been successfully employed in the treatment of IDs.Finally,the review discusses the current opportunities and challenges in this field and provides insights for the future clinical applications of PDVLNs in treating IDs.
基金supported by the National Natural Science Foundation of China(Nos.81871161 and 32301082)Fujian Provincial Health Technology Project for their attribution from(No.2023GGB01)the Natural Science Foundation of Fujian Province(No.2021J01343).
文摘Polycystic ovarian syndrome(PCOS)is a frequently encountered disease with hormonal and metabolic dysfunction in women of reproductive age.Nano-sized titanium dioxide(nTiO_(2))has been widely used in skin care products,paints,food,and cosmetics.In this study,we found a substantial elevation of Ti in the follicular fluid of females diagnosed with nonhyperandrogenic PCOS(non-HA PCOS).Therefore,we orally subjected female rats to nTiO_(2) at 10 and 100 mg/kg body weight for 10 weeks.We found that nTiO_(2) triggered non-HA PCOS-like symptoms,including the increase of cystic follicles,estrous cycle disorder,and abnormal ovulation,but without high testosterone.By transcriptome sequencing,we identified that nTiO_(2) exposure inhibited the steroid hormone synthesis and activated the proapoptotic pathways.Upon validating these altered pathways,we discovered that aromatase(Cyp19a1)overexpression contributed to the decline in testosterone/estradiol ratio.Mean-while,the apoptosis of granulosa cells was increased by nTiO_(2) exposure,which might result in the disorder of follicular development.This study presents the evidences revealing a pronounced correlation between nTiO_(2) exposure and the emergence of non-HA PCOS,urging people to promote the safe utilization of nTiO_(2).
基金financial support from the National Natural Science Foundation of China(Nos.82202354,U20A20338,82201247)The Summit Advancement Disciplines of Zhejiang Province(Wenzhou Medical University-Pharmaceutics)the Key R&D Program of Zhejiang Province(No.2021C04019)。
文摘Tumor heterogeneity and diversity significantly undermine the effectiveness of monotherapy.Collaborative therapy emerges as a promising approach to mitigate tumor recurrence resulting from monotherapy.Combining chemodynamic therapy(CDT)with photothermal therapy(PTT)offers a compelling solution for eradicating residual tumor cells post-PTT.In this study,we harness the Fenton-like response facilitated by glucose oxidase(GOD)and the mild hyperthermia induced by polyethyleneimine(PEI)functionalized nitrogen-containing graphene oxide to enhance tumor therapy through a metal-free bionic nanozyme.GOD catalyzes a substantial amount of hydrogen peroxide,and,with the carrier's involvement,triggers a Fenton-like reaction,yielding a wealth of hydroxyl radicals.These hydroxyl radicals effectively target tumor cells following photothermal action,bolstering CDT and culminating in a bidirectional amplification treatment that effectively prevents tumor recurrence and metastasis.This research amalgamates the physical and chemical attributes of nanomaterials with the unique characteristics of the tumor microenvironment,presenting a compelling and efficacious alternative for tumor treatment.
基金financial support from National Science Foundation of China(Grant No.52402445)the Natural Science Foundation of Jiangsu Province(Grant No.BK20241325)the startup support from Southeast University(Grant No.RF1028623337)。
文摘Solid-to-solid interfacial issues are one of the most intractable problems hindering the practical application of all-solid-state batteries(ASSBs).The interfacial instability behaviors caused by the rough interface between lithium anode and solid electrolyte(SE)involve complicated electro-chemo-mechanics interplays and their quantitative relationships still remain unclear.The three-dimensional electro-chemomechanical coupled model with randomly generated rough lithium-SE interface is developed in this study to investigate the effects of interface roughness on the interfacial failure behaviors.Results demonstrate that the existence of a rough lithium-SE interface causes the highly concentrated strain,GPa-level stress,and localized current density at the protruding tips,probably inducing dendrite formation and interface cracking.The interface roughness effect is much more pronounced in lithium anode than graphite anode due to their different Li storage mechanisms,i.e.,surface deposition and Li intercalation.Excessive stack pressure(>50 MPa)magnifies the stress effect on overpotential to enlarge the current density localization and deteriorate the interfacial instability issues.Reducing interface roughness through surface treatment,together with regulation of external operation conditions,can effectively improve interfacial stability performance.The results provide an in-depth understanding of the underlying electro-chemo-mechanical coupling mechanism caused by the rough anode-SE interface and bring more insights into further improvement of ASSBs'enhanced reliability and longevity.
基金financially supported by the open fund of Key Lab-oratory of Exploration Technologies for Oil and Gas Resources(Yangtze University),Ministry of Education,NO PI2023-03the open foundation of the National Engineering Laboratory for Exploration and Develop-ment of Low-Permeability Oil&Gas Fields and the National Natural Science Foundation of China(No.42474159).
文摘The pore structures of the Majiagou Formation in the Ordos Basin are complex,featuring micro-and nano-scale intra-crystalline and inter-crystalline pores that significantly impact hydrocarbon storage and flow.Precisely characterizing the rock internal structures is crucial for reservoir exploration and development.However,it is difficult to accurately characterize the pore structure of rock using traditional imaging methods to meet the simulation requirements.In this context,this study focuses on high-resolution 3D digital core reconstruction using the SliceGAN model.Specifically,the Modular Automated Processing System(MAPS)image and Quanti-tative Evaluation of Minerals by Scanning Electron Microscopy(QEMSCAN)image were combined to divide MAPS into three categories:pore,dolomite,and calcite.Then,through the SliceGAN algorithm,the 3D digital core was reconstructed.To evaluate the reconstruction,the auto-correlation function,two-point probability function,porosity,mineral content,and specific surface area were employed.The results show that the SliceGAN can effectively capture the micro-features in the core,and the internal structure of the generated core was consistent with that of the original core.This study provided a new sight for reconstructing cores with complex pore structures and strong heterogeneity and innovatively supports tight carbonate reservoir characterization and evaluation.
基金financially supported by the Program for the Development of Science and Technology of Jilin Province(Nos.YDZJ202201ZYTS313,YDZJ202201ZYTS395,20240402072GH,and 20240101004JJ)the National Natural Science Foundation of China(Nos.22201097 and 52171210)。
文摘Improving the surface atoms utilization efficiency of catalysts is extremely important for large-scale H_(2)production by electrochemical water splitting,but it remains a great challenge.Herein,we reported two kinds of Mo O_(3)-polyoxometalate hybrid nanobelt superstructures(MoO_(3)-POM HNSs,POM=PW_(12)O_(40)and Si W_(12)O_(40))using a simple hydrothermal method.Such superstructure with highly uniform nanoparticles as building blocks can expose more surface atoms and emanate increased specific surface area.The incorporated POMs generated abundant oxygen vacancies,improved the electronic mobility,and modulated the surface electronic structure of MoO_(3),allowing to optimize the H^(*)adsorption/desorption and dehydrogenation kinetics of catalyst.Notably,the as-prepared MoO_(3)-PW_(12)O_(40)HNSs electrodes not only displayed the low overpotentials of 108 mV at 10 mA/cm^(2)current density in 0.5 mol/L H_(2)SO_(4)electrolyte but also displayed excellent long-term stability.The hydrogen evolution reaction(HER)performance of MoO_(3)-POM superstructures is significantly better than that of corresponding bulk materials MoO_(3)@PW_(12)O_(40)and Mo O_(3)@Si W_(12)O_(40),and the overpotentials are about 8.3 and 4.9 times lower than that of single Mo O_(3).This work opens an avenue for designing highly surface-exposed catalysts for electrocatalytic H_(2)production and other electrochemical applications.
基金financially supported by the National Key Re-search and Development Program of China(No.2017YFB0701801)。
文摘Partial phase transformation in NiTi-based refrigerants usually enables efficient and durable elastocaloric cooling,but its thermomechanical behavior with varying temperatures remains unclear.Keeping this in view,the elastocaloric effect of NiTi under incomplete transformation across 15-100℃ is investigated and a superelastic deformation window between 25 and 85℃ is identified.Synchronous infrared thermography and digital image correlation,and an innovative macro-micro phase-field model are employed to examine martensitic transformation and elastocaloric properties of NiTi within the superelastic window.Experimental and simulated results consistently reveal that the spatiotemporal thermal profiles correlate with Lüders strain band evolution.As superelastic deformation temperature increases,strain localization intensifies,with Lüders bands favoring an inward strain growth over an outward expansion,resulting in a smaller yet more deformed martensitic transformation zone.The aggravated strain inhomogeneity makes the local endothermic undercooling tested at 85℃ up to about twice(−30.05℃)that at 25℃(−15.32℃),boosting the global cooling capacity by 65%,despite constant strain.The seeming contradiction between the larger elastocaloric effect and the narrower apparent martensitic transformation zone is elucidated by recourse to the simulations.It is found that the martensitic transformation within the Lüders bands is incomplete,proceeding in a macroscopically uniform but microscopically heterogeneous manner.Elevated temperatures within the superelastic window increase the transformed volume fraction and enhance martensitic transformation,thereby strengthening the global caloric effect.The work sheds light on the interplay between partial martensitic transformation and thermal behavior in NiTi under varying superelastic deformation temperatures,providing insights for advanced elastocaloric cooling applications.
基金supported by Zhejiang Provincial College Students’Science and Technology Innovation Program and Xinmiao Talent Plan(No:2024R413C093)。
文摘Background:Viral myocarditis(VMC)is an inflammatory myocardial disease induced by viral infections,and currently,there are no effective targeted treatments available.Cyclovirobuxine D(CVB-D),a major alkaloid extracted from the traditional eastern medicinal plant Cephalotaxus,has been clinically used in the treatment of arrhythmias and coronary heart disease.However,its therapeutic effects and underlying mechanisms in VMC remain unclear.This study aims to investigate the cardioprotective effects of CVB-D in a murine model of viral myocarditis induced by Coxsackievirus B3(CVB3)and to elucidate the molecular mechanisms involved in its effects.Method:A murine model of VMC was established by infecting C57BL/6J mice with CVB3.The mice were randomly assigned to three groups:the sham-operated group,the model group,and the CVB-D treatment group.After 14 days of intervention,the general health status of the mice was evaluated.Histopathological changes in myocardial tissue and myocardial cell cross-sectional areas were examined using Hematoxylin and Eosin(H&E)staining.Cardiac function was assessed via echocardiography.Additionally,network pharmacology was employed to identify VMC-related targets and signaling pathways,which were further analyzed to hypothesize the potential therapeutic mechanisms of CVB-D in VMC.Result:CVB-D exerts significant protective effects against CVB3-induced VMC.The underlying mechanisms may be associated with mitochondrial energy metabolism,the electron transport chain,and oxidative phosphorylation processes.This study provides experimental evidence supporting the potential of CVB-D as a therapeutic agent for VMC.
基金Supported by the National Science&Technology Fundamental Resources Investigation Program of China(No.2022FY100603)the Key Project of the NSFC-Shandong Joint Fund(No.U2106208)+1 种基金the National Key Research and Development Program of China(No.2023YFC3108200)the Taishan Scholars Program(No.tsqn202211263)。
文摘Coral reefs support a wide range of organisms in the world,including jellyfish and their benthic relatives.However,quantifying the biodiversity of these organisms in reefs is a challenge because of their uneven distribution and cryptic early life stages,requiring the validation of alternative techniques for biodiversity assessment.Here,the biodiversity and spatial distribution patterns of jellyfish and their benthic relatives,from the Scyphozoa,Hydrozoa,and Ctenophora taxa(hereafter referred to as SHC),were investigated in the coral reefs of Xisha,China,using environmental DNA(eDNA)metabarcoding technology by collecting shallow seawater,mesophotic seawater,and sediment samples.One-hundred and eighty-eight SHC species spanning two phyla(Cnidaria and Ctenophora),three classes,11 orders,65 families,and 104 genera were identified,among which hydrozoans were the most dominant taxa,accounting for 89.81% of all SHC species.SHC species showed low connectivity between shallow and mesophotic habitats,presenting a clear vertical distribution pattern in coral reefs.In the mesophotic coral ecosystems(MCEs),140 SHC species(84.34%)were detected,of which 39.76% were exclusive to MCEs,with Zanclea sp.1,Orthopyxis integra,and Fabienna sphaerica being the dominant species.Additionally,although SHC diversity in seawater was higher than that in the sediment samples,22 species were identified only in the sediment samples,indicating that sediment eDNA may represent a valuable supplementary tool for the investigation of SHC communities in hot spots.In addition to revealing the vast diversity of SHC species occupying coral reef ecosystems in the Xisha Islands,our findings confirm the potential of eDNA metabarcoding as an advanced tool for monitoring the biodiversity of cryptic species.
基金supported by the Natural Science Foundation of Anhui Province,China(2008085qc118)the National Natural Science Foundation of China(U19A2021)+1 种基金the Major Science and Technology Special Project of Anhui Province,China(S202003a06020035)the Jiangsu Collaborative Innovation Center for Modern Crop Production,China(JCIC-MCP)。
文摘Global warming is primarily characterized by asymmetric temperature increases,with greater temperature rises in winter/spring and at night compared to summer/autumn and the daytime.We investigated the impact of winter night warming on the top expanded leaves of the spring wheat cultivar Yangmai 18 and the semi-winter wheat cultivar Yannong 19 during the 2020-2021 growing season.Results showed that the night-time mean temperature in the treatment group was 1.27°C higher than the ambient temperature,and winter night warming increased the yields of both wheat cultivars,the activities of sucrose synthase and sucrose phosphate synthase after anthesis,and the biosynthesis of sucrose and soluble sugars.Differentially expressed genes(DEGs)were identified using criteria of P-value<0.05 and fold change>2,and they were subjected to Gene Ontology(GO)annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses.Genes differentially expressed in wheat leaves treated with night warming were primarily associated with starch and sucrose metabolism,amino acid biosynthesis,carbon metabolism,plant hormone signal transduction,and amino sugar and nucleotide sugar metabolism.Comparisons between the groups identified 14 DEGs related to temperature.These results highlight the effects of winter night warming on wheat development from various perspectives.Our results provide new insights into the molecular mechanisms of the response of wheat to winter night warming and the candidate genes involved in this process.
基金The 2024 Qinghai University Ideological-Political Education Project“Research on the Paths and Methods of Telling Qinghai’s Stories Well in the College English Teaching”(szzx2410)。
文摘Language learning and cultural communication are inherently intertwined and mutually reinforcing.In the context of college English instruction,emphasizing“telling China’s stories well”aligns with the principles of foreign language education and inevitably promotes cultural exchange and mutual understanding across different cultures through foreign language learning.Many researchers have explored ways of cultivating students’ability to communicate Chinese stories and spread Chinese culture in English.However,a few studies have been performed on exploring paths of incorporating regional culture into English language education.In particular,incorporating Qinghai’s indigenous cultural resources into college English teaching practice is rare.Therefore,a survey was conducted to ascertain the present situation concerning integrating Qinghai’s regional culture into college English teaching practice at Qinghai University.Based on the data from the survey,the present study discusses the results and implications obtained during the present teaching process and probes into practical strategies for the infiltration of Qinghai’s regional culture into college English teaching from the dimensions of curriculum,teaching materials,approaches,teachers’professional development,and teaching evaluation,intending to achieve the goals of language teaching and cultural education in the college English course.
