[ Objective ] This study aimed to establish and optimize the ISSR-PCR reaction system and amplification process for Elymus sibiricus L. , to provide sci- entific basis for exploring the genetic diversity of E. sibiric...[ Objective ] This study aimed to establish and optimize the ISSR-PCR reaction system and amplification process for Elymus sibiricus L. , to provide sci- entific basis for exploring the genetic diversity of E. sibiricus germplasm resources. [ Method] Orthogonal design and single factor test were applied to establish the ISSR-PCR reaction system of E. sibiricus, optimize the influencing factors including Taq DNA polymerase, DNA template concentration, Mg2 ＋ , dNTP, primer concentration, and screen the annealing temperature, number of cycles and extension time. [ Result ] The optimal reaction system for ISSR analysis contains 0.2 mmol/L dNTPs, 0.2 μmol/L ISSR primers, 1.5 U of Taq DNA polymerase, 2.5 μl of 10 × PCR Buffer, 1.5 mmol/L MgC12 and 40 ng of template DNA in 25 μl total volume; the amplification was conducted with 35 cycles and extension time of 90 s. [ Conclusion] ISSR-PCR reaction system for E. sibiricus was established and optimized, and then verified using two E. sibiricus germplasrns, demonstrating that the ISSR-PCR reaction system is stable and can be used for the genetic analysis of E. sibiricus.展开更多
For patients with locally advanced gastric cancer(LAGC),the effectiveness of perioperative chemotherapy can be significantly improved by adding programmed cell death protein 1(PD-1)inhibitors during the neoadjuvant ph...For patients with locally advanced gastric cancer(LAGC),the effectiveness of perioperative chemotherapy can be significantly improved by adding programmed cell death protein 1(PD-1)inhibitors during the neoadjuvant phase.However,a substantial percentage of patients with a programmed death-ligand 1 combined positive score(PD-L1 CPS)<1 or microsatellite stable(MSS)status may still not experience benefits.展开更多
基金Supported by Project of Collection,Cataloguing and Utilization of Perennial Forage Grass Germplasm Resources(NB2012-2130135-33)Conservation Project for Forage Grass Germplasm Resources
文摘[ Objective ] This study aimed to establish and optimize the ISSR-PCR reaction system and amplification process for Elymus sibiricus L. , to provide sci- entific basis for exploring the genetic diversity of E. sibiricus germplasm resources. [ Method] Orthogonal design and single factor test were applied to establish the ISSR-PCR reaction system of E. sibiricus, optimize the influencing factors including Taq DNA polymerase, DNA template concentration, Mg2 ＋ , dNTP, primer concentration, and screen the annealing temperature, number of cycles and extension time. [ Result ] The optimal reaction system for ISSR analysis contains 0.2 mmol/L dNTPs, 0.2 μmol/L ISSR primers, 1.5 U of Taq DNA polymerase, 2.5 μl of 10 × PCR Buffer, 1.5 mmol/L MgC12 and 40 ng of template DNA in 25 μl total volume; the amplification was conducted with 35 cycles and extension time of 90 s. [ Conclusion] ISSR-PCR reaction system for E. sibiricus was established and optimized, and then verified using two E. sibiricus germplasrns, demonstrating that the ISSR-PCR reaction system is stable and can be used for the genetic analysis of E. sibiricus.
基金supported by Jiangsu Hengrui Medicine Co.,Ltd.&Innovent Biologics(Suzhou)Co.,Ltd,the Fundamental Research Funds for the Central Universities of Lanzhou University(Nos.lzujbky-2022-ey04 and lzujbky-2023-eyt04)the Science and Technology Program of Gansu Province,China(Nos.21JR1RA144 and 22JR5RA961)the Cuiying Scientific and Technological Innovation Program of Lanzhou University Second Hospital,China(Nos.CY2020-MS10 and CY2023-QN-A02).
文摘For patients with locally advanced gastric cancer(LAGC),the effectiveness of perioperative chemotherapy can be significantly improved by adding programmed cell death protein 1(PD-1)inhibitors during the neoadjuvant phase.However,a substantial percentage of patients with a programmed death-ligand 1 combined positive score(PD-L1 CPS)<1 or microsatellite stable(MSS)status may still not experience benefits.
