Occurrence and development of lymphocystis cells in flounder Paralichthys olivaceus, and some histochemical features are studied using light and electron microscopy and histochemical methods. Fibroblasts engulf virus ...Occurrence and development of lymphocystis cells in flounder Paralichthys olivaceus, and some histochemical features are studied using light and electron microscopy and histochemical methods. Fibroblasts engulf virus particles forming phagocytosis vacuole in the cytoplasm. The infected cells proliferate and turn round and hypertrophied; basophilic inclusion bodies, Feulgen-positive, are present in the cytoplasm, and a hyaline capsule, containing acid ploysaccharide and protein, appears outside cell membrane. The mature lymphocystis cells possess numerous virus particles (200～220nm in diameter with a central core 120～140nm) and high electron-dense inclusions with budding virions at the surface in the cytoplasm, and show margination of chromatin. Moderate electron density particles (70～80nm in diameter) fill out perinuclear cisterna and arrange in crystalloid arrays in nucleus, liberated into the cytoplasm by rupture of nuclear membrane. No virus particles are observed in nucleus. Therefore, inclusion bodies are the sites of virus assembly and maturity, while replication processes of lymphocystis virus are involved in both nuclear and cytoplasmic phases. Besides, strongly basophilic link-like structures are present between adjacent cells or through connective tissue, and virus particles are observed in somewhere of the capsule, thus where may be the spread route of virus from one cell to another or to the environment. The senile lymphocystis cells release virus by the rupture of cells.展开更多
Data is clear that many childeren with cancer at the end of life suffer substantiall.Treatment was viewed as successful in only 27% of the patients.Pain is children who are dying os cacer can be complex and challengin...Data is clear that many childeren with cancer at the end of life suffer substantiall.Treatment was viewed as successful in only 27% of the patients.Pain is children who are dying os cacer can be complex and challenging to mannage.Children and parents are equal partners with members of the health care team in managing the patient's pain.Prevention and alleviation of pain is a primary goal of care in the child dying of cancer.Children dying of cancer may require aggressive dosing of analgesics.Medications that do not have a dose maximum should be escalated,sometimes rapidly ,to achieve adequate pain control or to maintain pain control when tolerance has occurred.The nurse's s role in caring for children who are in pain at the end-of-life includes assessment,identifying expected outcomes,and plan-ning ,performing,and evaluating interventions.展开更多
A new genomic DNA encoding a member of Gastrodia antifungal protein family is isolated and sequenced. This gene contains a 510 bp open reading frame and 531 bp promoter region without introns. Sequence analysis indica...A new genomic DNA encoding a member of Gastrodia antifungal protein family is isolated and sequenced. This gene contains a 510 bp open reading frame and 531 bp promoter region without introns. Sequence analysis indicates that a 28 amino acids signal peptide exists at the N terminal. It shows high sequence homology with the mannose binding lectins from Epipactis helleborine, Listera ovata and Cymbidium hybrid. A putative TATA box and transcription start site is detected in the promoter region.展开更多
Taking "three dimensions" of natural language, graphic language and symbolic language as tools, this paper explores the teaching of function symmetry in senior high school. The axisymmetric and central symme...Taking "three dimensions" of natural language, graphic language and symbolic language as tools, this paper explores the teaching of function symmetry in senior high school. The axisymmetric and central symmetry of the function itself and the axisymmetric and central symmetry between the two functions are explored respectively. On the basis of studying the problem of function symmetry, the universal applicability of the above three tools in high school mathematics teaching is further discussed and prospected. In high school, the symmetry, as one of the basic properties of functions, is not only the content that teachers should focus on in teaching, but also a key knowledge module that it is difficult for most students to understand deeply. Its importance need not be repeated.展开更多
基金国家高技术研究发展计划(863计划)，国家自然科学基金，教育部科学技术研究项目，the Cheung Kong Scholars Program in China
文摘Occurrence and development of lymphocystis cells in flounder Paralichthys olivaceus, and some histochemical features are studied using light and electron microscopy and histochemical methods. Fibroblasts engulf virus particles forming phagocytosis vacuole in the cytoplasm. The infected cells proliferate and turn round and hypertrophied; basophilic inclusion bodies, Feulgen-positive, are present in the cytoplasm, and a hyaline capsule, containing acid ploysaccharide and protein, appears outside cell membrane. The mature lymphocystis cells possess numerous virus particles (200～220nm in diameter with a central core 120～140nm) and high electron-dense inclusions with budding virions at the surface in the cytoplasm, and show margination of chromatin. Moderate electron density particles (70～80nm in diameter) fill out perinuclear cisterna and arrange in crystalloid arrays in nucleus, liberated into the cytoplasm by rupture of nuclear membrane. No virus particles are observed in nucleus. Therefore, inclusion bodies are the sites of virus assembly and maturity, while replication processes of lymphocystis virus are involved in both nuclear and cytoplasmic phases. Besides, strongly basophilic link-like structures are present between adjacent cells or through connective tissue, and virus particles are observed in somewhere of the capsule, thus where may be the spread route of virus from one cell to another or to the environment. The senile lymphocystis cells release virus by the rupture of cells.
文摘Data is clear that many childeren with cancer at the end of life suffer substantiall.Treatment was viewed as successful in only 27% of the patients.Pain is children who are dying os cacer can be complex and challenging to mannage.Children and parents are equal partners with members of the health care team in managing the patient's pain.Prevention and alleviation of pain is a primary goal of care in the child dying of cancer.Children dying of cancer may require aggressive dosing of analgesics.Medications that do not have a dose maximum should be escalated,sometimes rapidly ,to achieve adequate pain control or to maintain pain control when tolerance has occurred.The nurse's s role in caring for children who are in pain at the end-of-life includes assessment,identifying expected outcomes,and plan-ning ,performing,and evaluating interventions.
文摘A new genomic DNA encoding a member of Gastrodia antifungal protein family is isolated and sequenced. This gene contains a 510 bp open reading frame and 531 bp promoter region without introns. Sequence analysis indicates that a 28 amino acids signal peptide exists at the N terminal. It shows high sequence homology with the mannose binding lectins from Epipactis helleborine, Listera ovata and Cymbidium hybrid. A putative TATA box and transcription start site is detected in the promoter region.
文摘Taking "three dimensions" of natural language, graphic language and symbolic language as tools, this paper explores the teaching of function symmetry in senior high school. The axisymmetric and central symmetry of the function itself and the axisymmetric and central symmetry between the two functions are explored respectively. On the basis of studying the problem of function symmetry, the universal applicability of the above three tools in high school mathematics teaching is further discussed and prospected. In high school, the symmetry, as one of the basic properties of functions, is not only the content that teachers should focus on in teaching, but also a key knowledge module that it is difficult for most students to understand deeply. Its importance need not be repeated.
