Ovule and seed developments are crucial processes during plant growth, which are affected by different signaling pathways. In this paper, we demonstrate that the brassinosteroid （BR） signal is involved in ovule init...Ovule and seed developments are crucial processes during plant growth, which are affected by different signaling pathways. In this paper, we demonstrate that the brassinosteroid （BR） signal is involved in ovule initiation and development. Ovule and seed numbers are significantly different when comparing BR-related mutants to wild-type con-trols. Detailed observation indicates that BR regulates the expression level of genes related to ovule development, includ-ing HLL, ANT, and AP2, either directly by targeting the promoter sequences or indirectly via regulation by BR-induced transcription factor BZR1. Also, Western blot demonstrates that the dephosphorylation level of BZR1 is consistent with ovule and seed number. The intragenic bzrl-lD suppressors bzs247 and bzs248 have much fewer ovules and seeds than bzrl-lD, which are similar to wild-type, suggesting that the phenotype can be rescued. The molecular and genetic experi-ments confirm that BZR1 and AP2 probably affect Arabidopsis ovule number determination antagonistically.展开更多
文摘Ovule and seed developments are crucial processes during plant growth, which are affected by different signaling pathways. In this paper, we demonstrate that the brassinosteroid （BR） signal is involved in ovule initiation and development. Ovule and seed numbers are significantly different when comparing BR-related mutants to wild-type con-trols. Detailed observation indicates that BR regulates the expression level of genes related to ovule development, includ-ing HLL, ANT, and AP2, either directly by targeting the promoter sequences or indirectly via regulation by BR-induced transcription factor BZR1. Also, Western blot demonstrates that the dephosphorylation level of BZR1 is consistent with ovule and seed number. The intragenic bzrl-lD suppressors bzs247 and bzs248 have much fewer ovules and seeds than bzrl-lD, which are similar to wild-type, suggesting that the phenotype can be rescued. The molecular and genetic experi-ments confirm that BZR1 and AP2 probably affect Arabidopsis ovule number determination antagonistically.
