The PlnC encoded by plnC is an essential response regulator in Plantaricin biosynthesis well-studied in Lactiplantibacillus plantarum.Since the known effects of plnC are not exhaustive,it is crucial to perfect a preci...The PlnC encoded by plnC is an essential response regulator in Plantaricin biosynthesis well-studied in Lactiplantibacillus plantarum.Since the known effects of plnC are not exhaustive,it is crucial to perfect a precise regulatory mechanism for plnC.Overexpression of plnC was found to transiently reduce bacterial activity during the logarithmic growth phase,however,it significantly increased the expression levels of bacteriocin genes within the pln locus by 1.45-2.35 times.After 16 h of incubation,the overexpression of plnC significantly increased the diameter of the inhibition zone from 10.89±0.36 mm to 12.29±0.33 mm,indicating a notable enhancement in bacteriocin activity(P<0.05).The proteomic analysis further revealed that plnC affected multiple carbohydrate metabolic pathways,QS and HIF-1 signaling pathways,IMP/UMP biosynthesis,and ribosomal proteins,which resulted in promoting the synthesis of nucleotide precursors and exopolysaccharides,and accelerating ribosome translation rate.And the up-regulated synthesis of long-chain acyl-CoA is an important component that may inhibit PlnD(an inhibitory regulator opposite PlnC)during Plantaricin biosynthesis.These offer a critical basis for forward Plantaricin research and application.展开更多
Background:Traditional Chinese medicine(TCM),which have potent clinical therapeutic efficacy,are commonly used in treating various diseases in China.The TCM include not only the single bioactive molecules like tanshin...Background:Traditional Chinese medicine(TCM),which have potent clinical therapeutic efficacy,are commonly used in treating various diseases in China.The TCM include not only the single bioactive molecules like tanshinone,salvianolic acid and curcumin,but also those Chinese herbal formulas.In the past decades,the researchers keep working on the scientific basis of these herbs.Clearly,TCM herbs hit diverse biological targets associated with multiple diseases.Growth factors including VEGFs,FGFs,PDGFs are a class of biological targets in human bodies,which play vital roles in many physiological processes.A number of studies demonstrated either inhibiting or enhancing effects of the TCM on growth factors-triggered biological activities.Objective:Herein,we review the effects of TCM on growth factors,attempting to explore the roles of TCM in regulating the functions of growth factors.Methods:To review the effects of TCM on growth factors,we focused on studies involved in the therapeutic use and pharmacological activity of TCM and performed a comprehensive search of several scientific databases using various relevant keywords.Some inclusion and exclusion criteria were strictly followed to obtain the most relevant articles.Results:This review screened 289 papers,of which 93 provided in-depth analyses of TCM and growth factors,focusing on the effects of TCM targeting growth factors on inflammation,Alzheimer’s disease,tumor growth,and liver fibrosis.In addition,development of dressings or patches combining TCM and growth factors to enhance the activity of growth factors in clinic usage is also discussed.Conclusions:These findings indicate that various TCM extracts,including polysaccharides,and TCM formulations play potentially beneficial roles,through effects on the growth factors in the treatment of a range of diseases and can be used to treat inflammation and other diseases.展开更多
Low yield is a significant bottleneck preventing widespread application of bacteriocins from lactic acid bacteria(LAB).Co-culturing different species of LAB is an effective strategy to enhance bacteriocin production.T...Low yield is a significant bottleneck preventing widespread application of bacteriocins from lactic acid bacteria(LAB).Co-culturing different species of LAB is an effective strategy to enhance bacteriocin production.This study aimed to investigate the mechanisms underlying this enhancement,focusing on the metabolic pathways involved and identifying effector metabolites that improve the yield of bacteriocins from LAB.The results indicated that bacteriocin production was highest when Lactiplantibacillus plantarum ZY-1 was co-cultured with Limosilactobacillus fermentum RC4 at a seed liquid age of 16 h.Metabolomic analysis of co-cultured and separately cultured LAB revealed 984 significantly differentially abundant metabolites,including organic acids,lipids,amino acids,and vitamins.The primary metabolic pathways involved were amino acid metabolism,ABC transporters,and purine metabolism.Co-culture enhanced the synthesis of metabolites including ascorbic acid,N-acetyl-L-glutamic acid,butyryl trihexyl citrate,N-acetyl aspartate,and L-gulonolactone,as demonstrated through metabolic analysis and validation experiments.Compared to the control group,the addition of a mixture containing 0.2%ascorbic acid,0.2% N-acetyl-L-glutamic acid,0.1%butyryl trihexyl citrate,0.1% N-acetyl aspartic acid,and 0.3% L-gulonolactone to the co-culture system demonstrated a 1.9-fold enhancement in the inhibition zone diameter.Similarly,a combination of 0.3%ascorbic acid,0.3% butyryl trihexyl citrate,0.1% N-acetyl aspartate,and 0.1%L-gulonolactone achieved a 2.1-fold amplification in bacteriostatic activity within the monoculture system.This is the first report on the mechanism of enhancing bacteriocin production via co-culture of different LAB species.Furthermore,a strategy to augment bacteriocin production was developed by adding key metabolites to monocultured and co-cultured LAB.展开更多
基金the financial support of the National Key Research and Development Program of China(2022YFD2100603,2022YFD2100600)the Natural Science Foundation of Zhejiang Province for Diatinguished Young Scholars(LR23C200001)the National Natural Science Foundation of China(32472357,32072195).
文摘The PlnC encoded by plnC is an essential response regulator in Plantaricin biosynthesis well-studied in Lactiplantibacillus plantarum.Since the known effects of plnC are not exhaustive,it is crucial to perfect a precise regulatory mechanism for plnC.Overexpression of plnC was found to transiently reduce bacterial activity during the logarithmic growth phase,however,it significantly increased the expression levels of bacteriocin genes within the pln locus by 1.45-2.35 times.After 16 h of incubation,the overexpression of plnC significantly increased the diameter of the inhibition zone from 10.89±0.36 mm to 12.29±0.33 mm,indicating a notable enhancement in bacteriocin activity(P<0.05).The proteomic analysis further revealed that plnC affected multiple carbohydrate metabolic pathways,QS and HIF-1 signaling pathways,IMP/UMP biosynthesis,and ribosomal proteins,which resulted in promoting the synthesis of nucleotide precursors and exopolysaccharides,and accelerating ribosome translation rate.And the up-regulated synthesis of long-chain acyl-CoA is an important component that may inhibit PlnD(an inhibitory regulator opposite PlnC)during Plantaricin biosynthesis.These offer a critical basis for forward Plantaricin research and application.
基金supported by the National Natural Science Foundation of China(NSFC 82003922)Youth Innovation Team of Higher Education of Shandong Province(2021KJ094).
文摘Background:Traditional Chinese medicine(TCM),which have potent clinical therapeutic efficacy,are commonly used in treating various diseases in China.The TCM include not only the single bioactive molecules like tanshinone,salvianolic acid and curcumin,but also those Chinese herbal formulas.In the past decades,the researchers keep working on the scientific basis of these herbs.Clearly,TCM herbs hit diverse biological targets associated with multiple diseases.Growth factors including VEGFs,FGFs,PDGFs are a class of biological targets in human bodies,which play vital roles in many physiological processes.A number of studies demonstrated either inhibiting or enhancing effects of the TCM on growth factors-triggered biological activities.Objective:Herein,we review the effects of TCM on growth factors,attempting to explore the roles of TCM in regulating the functions of growth factors.Methods:To review the effects of TCM on growth factors,we focused on studies involved in the therapeutic use and pharmacological activity of TCM and performed a comprehensive search of several scientific databases using various relevant keywords.Some inclusion and exclusion criteria were strictly followed to obtain the most relevant articles.Results:This review screened 289 papers,of which 93 provided in-depth analyses of TCM and growth factors,focusing on the effects of TCM targeting growth factors on inflammation,Alzheimer’s disease,tumor growth,and liver fibrosis.In addition,development of dressings or patches combining TCM and growth factors to enhance the activity of growth factors in clinic usage is also discussed.Conclusions:These findings indicate that various TCM extracts,including polysaccharides,and TCM formulations play potentially beneficial roles,through effects on the growth factors in the treatment of a range of diseases and can be used to treat inflammation and other diseases.
基金support of the Natural Science Foundation of Zhejiang Province for Distinguished Young Scholars,China(LR23C200001)the National Natural Science Foundation of China(32472357,32072195)the National Key Research and Development Program of China(2022YFD2100600,2022YFD2100603).
文摘Low yield is a significant bottleneck preventing widespread application of bacteriocins from lactic acid bacteria(LAB).Co-culturing different species of LAB is an effective strategy to enhance bacteriocin production.This study aimed to investigate the mechanisms underlying this enhancement,focusing on the metabolic pathways involved and identifying effector metabolites that improve the yield of bacteriocins from LAB.The results indicated that bacteriocin production was highest when Lactiplantibacillus plantarum ZY-1 was co-cultured with Limosilactobacillus fermentum RC4 at a seed liquid age of 16 h.Metabolomic analysis of co-cultured and separately cultured LAB revealed 984 significantly differentially abundant metabolites,including organic acids,lipids,amino acids,and vitamins.The primary metabolic pathways involved were amino acid metabolism,ABC transporters,and purine metabolism.Co-culture enhanced the synthesis of metabolites including ascorbic acid,N-acetyl-L-glutamic acid,butyryl trihexyl citrate,N-acetyl aspartate,and L-gulonolactone,as demonstrated through metabolic analysis and validation experiments.Compared to the control group,the addition of a mixture containing 0.2%ascorbic acid,0.2% N-acetyl-L-glutamic acid,0.1%butyryl trihexyl citrate,0.1% N-acetyl aspartic acid,and 0.3% L-gulonolactone to the co-culture system demonstrated a 1.9-fold enhancement in the inhibition zone diameter.Similarly,a combination of 0.3%ascorbic acid,0.3% butyryl trihexyl citrate,0.1% N-acetyl aspartate,and 0.1%L-gulonolactone achieved a 2.1-fold amplification in bacteriostatic activity within the monoculture system.This is the first report on the mechanism of enhancing bacteriocin production via co-culture of different LAB species.Furthermore,a strategy to augment bacteriocin production was developed by adding key metabolites to monocultured and co-cultured LAB.
