Background:Therapeutic responses of breast cancer vary among patients and lead to drug resistance and recurrence due to the heterogeneity.Current preclinical models,however,are inadequate for predicting individual pat...Background:Therapeutic responses of breast cancer vary among patients and lead to drug resistance and recurrence due to the heterogeneity.Current preclinical models,however,are inadequate for predicting individual patient responses towards different drugs.This study aimed to investigate the patient-derived breast cancer culture models for drug sensitivity evaluations.Methods:Tumor and adjacent tissues from female breast cancer patients were collected during surgery.Patient-derived breast cancer cells were cultured using the conditional reprogramming technique to establish 2D models.The obtained patient-derived conditional reprogramming breast cancer(CRBC)cells were subsequently embedded in alginate-gelatin methacryloyl hydrogel microspheres to form 3D culture models.Comparisons between 2D and 3D models were made using immunohistochemistry(tumor markers),MTS assays(cell viability),flow cytometry(apoptosis),transwell assays(migration),and Western blotting(protein expression).Drug sensitivity tests were conducted to evaluate patient-specific responses to anti-cancer agents.Results:2D and 3D culture models were successfully established using samples from eight patients.The 3D models retained histological and marker characteristics of the original tumors.Compared to 2D cultures,3D models exhibited increased apoptosis,enhanced drug resistance,elevated stem cell marker expression,and greater migration ability—features more reflective of in vivo tumor behavior.Conclusion:Patient-derived 3D CRBC models effectively mimic the in vivo tumor microenvironment and demonstrate stronger resistance to anti-cancer drugs than 2D models.These hydrogel-based models offer a cost-effective and clinically relevant platform for drug screening and personalized breast cancer treatment.展开更多
Lead zirconate titanate(PbZr_(x)Ti_(1-x)O_(3),PZT)ferroelectric films possess remarkable characteristics such as high residual polarization,high dielectric constant,and high piezoelectric coefficient and have great ap...Lead zirconate titanate(PbZr_(x)Ti_(1-x)O_(3),PZT)ferroelectric films possess remarkable characteristics such as high residual polarization,high dielectric constant,and high piezoelectric coefficient and have great application prospects in modern electronics,communications,medical care,and military fields.At present,the microstructure changes of PZT ferroelectric thin films have a significant impact on their electrical properties.Therefore,this work summarizes the influences of geometric structure(thickness,porosity),composition structure(Zr/Ti ratio,doping),and grain structure(grain size,grain boundaries,orientation)on the electrical properties of PZT ferroelectric thin films.The results show that the changes in thickness and porosity have a significant impact on the electrical properties of PZT ferroelectric films.Especially,the actual application scenarios and preparation processes determine the required geometric dimensions and structures of PZT ferroelectric films.The Zr/Ti ratio and doping mainly affect the electrical properties by influencing the phase composition of PZT ferroelectric films.The changes in grain size,boundary structure,and orientation dependence mainly have a certain degree of influence on the domain response and domain switching behavior of PZT ferroelectric thin films.In conclusion,different structures have different influence effects on the dielectric,ferroelectric,and piezoelectric properties of PZT ferroelectric films.The way the tiny structure affects how PZT thin films work was shown,helping to guide the design of ferroelectric thin film devices.In order to further study and apply piezoelectric ceramic devices,it is crucial to have an in-depth understanding of the relationship between the structure and performance of piezoelectric ceramic devices.展开更多
Mitochondria are a novel and promising therapeutic target for diagnosis, treatment and prevention of a lot of human diseases such as cancer, metabolic diseases and neurodegenerative disease. Owing to the mitochondrial...Mitochondria are a novel and promising therapeutic target for diagnosis, treatment and prevention of a lot of human diseases such as cancer, metabolic diseases and neurodegenerative disease. Owing to the mitochondrial special bilayer structure and highly negative potential nature, therapeutic molecules have multiple difficulties in reaching mitochondria. To overcome multiple barriers for targeting mitochondria, the researchers developed various pharmaceutical preparations such as liposomes, polymeric nanoparticles and inorganic nanoparticles modified by mitochondriotropic moieties like dequalinium (DQA),triphenylphosphonium (TPP), mitochondrial penetrating peptides (MPPs) and mitochondrial protein import machinery that allow specific targeting.The targeted formulations exhibited enhanced pharmacological effect and better therapeutic effect than their untargeted counterpart both in vitro and in vivo. Nanocarriers may be used for bio-therapeutic delivery into specific mitochondria that possess a great potential treatment of mitochondria related diseases.展开更多
Blautia has attracted attention because of its potential efficacy in ameliorating host energy metabolism and inflammation.This study aims to investigate the influences of Blautia producta D4 on colitis induced by dext...Blautia has attracted attention because of its potential efficacy in ameliorating host energy metabolism and inflammation.This study aims to investigate the influences of Blautia producta D4 on colitis induced by dextran sulfate sodium(DSS)and to reveal the underlying mechanisms.Results showed that B.producta D4 intervention significantly relieved body weight loss,and suppressed the elevation of pro-inflammatory cytokines(including interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β))and excessive oxidative stress(myeloperoxidease(MPO)activity,superoxide dismutase(SOD)activity,glutathione peroxidase(GSH-Px)activity,and malondialdehyde(MDA)level)in colitis mice.Moreover,the concentrations of tight junction proteins(occludin,claudin-1,and ZO-1)related to the intestinal barrier were obviously elevated,and colitis-related TLR4/NF-κB pathway activation was remarkably inhibited after B.producta D4 intervention.The intestinal microbial disorder was evidently ameliorated by increasing the relative abundance of Clostridium sensu stricto 1,Bifidobacterium,GCA-900066225,Enterorhabdus,and reducing the relative abundance of Lachnospiraceae NK4A136 group.In conclusion,oral administration of B.producta D4 could ameliorate DSS-induced colitis by suppressing inflammatory responses,maintaining the intestinal barrier,inhibiting TLR4/NF-κB pathway,and regulating intestinal microbiota balance.These results are conducive to accelerate the development of B.producta D4 as a functional probiotic for colitis.展开更多
Levilactobacillus brevis FZU0713, a potential probiotic previously isolated from the traditional brewing process of Hongqu rice wine, may have the beneficial effects on improving lipid metabolism. This study aimed to ...Levilactobacillus brevis FZU0713, a potential probiotic previously isolated from the traditional brewing process of Hongqu rice wine, may have the beneficial effects on improving lipid metabolism. This study aimed to evaluate the in vivo protective effects and possible mechanism of L. brevis FZU0713 on the disturbance of lipid metabolism in hyperlipidemic rats fed a high-fat diet(HFD). Results showed that oral administration of L. brevis FZU0713 could significantly inhibit obesity, ameliorate the lipid metabolism disorder, including serum/liver biochemical parameters and hepatic oxidative stress in HFD-fed rats. Histopathological result also indicated that dietary intervention of L. brevis FZU0713 could reduce the accumulation of lipid droplets in liver induced by 8 weeks HFD feeding. Furthermore, L. brevis FZU0713 intervention significantly increased the fecal levels of short-chain fatty acids(SCFAs, including acetate, propionate, butyrate, isobutyrate, valerate and isovalerate)in HFD-fed rats, which may be closely related to the changes of intestinal microbial composition and metabolic function. Intestinal microbiota profiling by 16S rRNA gene sequencing revealed that L. brevis FZU0713 intervention significantly altered the relative abundance of Coprococcus, Butyricicoccus, Intestinimonas, Lachnospiraceae FCS020 group, Ruminococcaceae_NK4A214 group, Ruminococcaceae_UCG-005 and UCG-014 at genus levels. Based on Spearman's rank correlation coefficient, serum and liver lipid metabolism related biochemical parameters were positively correlated with genera Ruminococcus, Pediococcus and Lachnospiraceae, but negatively correlated with genera Pseudoflavonifractor, Butyricicoccus and Intestinimonas. Furthermore, liver metabolomics analysis demonstrated that L. brevis FZU0713 had a significant regulatory effect on the composition of liver metabolites in hyperlipidemic rats, especially the levels of some important biomarkers involved in the metabolic pathways of arachidonic acid metabolism, primary bile acid biosynthesis, amino sugar and nucleotide sugar metabolism, taurine and hypotaurine metabolism, biosynthesis of unsaturated fatty acid, fructose and mannose metabolism, tyrosine metabolism, etc. Additionally, oral administration of L. brevis FZU0713 significantly regulated the mR NA levels of liver genes(including Acat2, Acox1, Hmgcr, Cd36, Srebp-1c and Cyp7a1)involved in lipid metabolism and bile acid homeostasis. In conclusion, our findings provide the evidence that L. brevis FZU0713 has the potential to improve disturbance of lipid metabolism by regulating intestinal microflora and liver metabonomic profile. Therefore, L. brevis FZU0713 may be used as a potential probiotic strain to produce functional food to prevent hyperlipidemia.展开更多
Photonic crystals are periodic structural materials that have an impact on the propagation properties of photons.Due to their excellent optical,electrical and magnetic properties,their advantages and potential for app...Photonic crystals are periodic structural materials that have an impact on the propagation properties of photons.Due to their excellent optical,electrical and magnetic properties,their advantages and potential for applications in the above areas are gradually emerging.Therefore,an increasing number of researchers have focused on photonic crystals.In this paper,the characteristics of biological photonic crystal structures,such as those found in butterfly wings,sea mouse bristles,peacock feathers,melon jellyfish epidermal cells,and weevil exoskeletons,are described.The preparation methods of photonic crystals are systematically summarized(including the template method,self-assembly technology,electron beam evaporation coating technology,chemical vapor deposition technology,femtosecond laser two-photon technology,spin coating technology,and a variety of technology mixing),and the characteristics,advantages,and disadvantages of the different methods are compared.Furthermore,the development of photonic crystals in the field of sensors,solar cells,filters,and infrared stealth is discussed,demonstrateing the great development potential of photonic crystals.It is concluded that the realization of photonic crystals with high precision,high sensitivity,angle independence,and large-area uniform preparation is a key problem requiring urgent solution.Moreover,photonic crystals have potential development prospects in the fields of equipment stealth,new concept weapons,production,an daily life.展开更多
This study aimed to investigate microbial succession and metabolic dynamics during the traditional fermentation of Hongqu aged vinegar,and explore the core functional microbes closely related to the formation of flavo...This study aimed to investigate microbial succession and metabolic dynamics during the traditional fermentation of Hongqu aged vinegar,and explore the core functional microbes closely related to the formation of flavor components.Microbiome analysis demonstrated that Lactobacillus,Acetobacter,Bacillus,Enterobacter,Lactococcus,Leuconostoc and Weissella were the predominant bacterial genera,while Aspergillus piperis,Aspergillus oryzae,Monascus purpureus,Candida athensensis,C.xylopsoci,Penicillium ochrosalmoneum and Simplicillium aogashimaense were the predominant fungal species.Correlation analysis revealed that Acetobacter was positively correlated with the production of tetramethylpyrazine,acetoin and acetic acid,Lactococcus showed positive correlation with the production of 2-nonanone,2-heptanone,ethyl caprylate,ethyl caprate,1-hexanol,1-octanol and 1-octen-3-ol,C.xylopsoci and C.rugosa were positively associated with the production of diethyl malonate,2,3-butanediyl diacetate,acetoin,benzaldehyde and tetramethylpyrazine.Correspondingly,non-volatile metabolites were also detected through ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry.A variety of amino acids and functional dipeptides were identified during the traditional brewing of Hongqu aged vinegar.Correlation analysis revealed that Lactobacillus was significantly associated with DL-lactate,indolelactic acid,D-(+)-3-phenyllactic acid,pimelic acid,pregabalin and 3-aminobutanoic acid.This study is useful for understanding flavor formation mechanism and developing effective strategies for the suitable strains selection to improve the flavor quality of Hongqu aged vinegar.展开更多
Barley beverage contains many natural nutrients which is suitable for lactic acid bacteria(LAB)fermentation.The aim of this study was to explore the influences of Lacticaseibacillus casei 17301 fermentation on the bio...Barley beverage contains many natural nutrients which is suitable for lactic acid bacteria(LAB)fermentation.The aim of this study was to explore the influences of Lacticaseibacillus casei 17301 fermentation on the bioactive ingredients,volatile and non-volatile compounds,and physiological properties of barley beverages.Our results showed that the total polyphenols and flavonoids in fermented barley beverages remarkably increased compared to those in unfermented barley beverages.A total of 63 volatile compounds in fermented barley beverages at the different fermentation stages were detected using headspace solid-phase micro-extraction coupled with gas chromatography-mass spectrometry(HS-SPME-GC-MS),including 17 acids,16 ketones,11 aldehydes,and 7 alcohols.Furthermore,untargeted metabolomics analysis-based ultra-performance liquid chromatography quadrupole-time of flight mass spectrometry(UHPLC-QTOF-MS)revealed L.casei 17301 effectively altered the non-volatile compounds,especially indole-3-lactic acid,in barley beverages.Most importantly,the barley beverage fermented with L.casei 17301 had elevated the antioxidant and lipase inhibition activities compared to the unfermented barley beverage.Collectively,L.casei 17301 could ameliorated the functionality and flavor of the barley beverage and the flavor of the product.These results of this study provide a theoretical basis for the development of Lacticaseibacillus-fermented cereal products with beneficial physiological properties.展开更多
Acute liver injury(ALI)is one of the most prevalent liver diseases,which have become a major health threat worldwide.Here,the meaning of this research is to explore the ability of L.rhamnosus FJG1530 to alleviate ALI ...Acute liver injury(ALI)is one of the most prevalent liver diseases,which have become a major health threat worldwide.Here,the meaning of this research is to explore the ability of L.rhamnosus FJG1530 to alleviate ALI in mice.In this study,L.rhamnosus FJG1530 alleviated lipopolysaccharide(LPS)-induced ALI symptoms,including elevated transaminase(aspartate aminotransferase[AST],and alanine aminotransferase[ALT])levels,liver tissue damage,increased liver oxidative stress(superoxide dismutase[SOD],catalase[CAT],glutathione[GSH],and total antioxidant capacity[T-AOC]),and high concentration of inflammatory cytokines(tumor necrosis factor-α[TNF-α],interleukin-1β[IL-1β],and interleukin-6[IL-6]).Additionally,the mice treated with L.rhamnosus FJG1530 showed an upward trend in short-chain fatty acid content in the cecum,and alteration of the gut microbiota structure.The study also discovered that L.rhamnosus FJG1530 intervention improved the composition of liver metabolites,and these characteristic biomarkers mainly involving galactose metabolism,histidine metabolism,ascorbate and aldarate metabolism etc.In summary,L.rhamnosus FJG1530 has the ability to prevent the occurrence of ALI through impairing the inflammatory response,curbing liver oxidative stress,and adjusting the gut microbiota structure.展开更多
基金supported by the Natural Science Foundation of Guangdong Province(No.2021B1515120053)Guangdong Basic and Applied Basic Research Foundation(Grant No.2024A1515140166).
文摘Background:Therapeutic responses of breast cancer vary among patients and lead to drug resistance and recurrence due to the heterogeneity.Current preclinical models,however,are inadequate for predicting individual patient responses towards different drugs.This study aimed to investigate the patient-derived breast cancer culture models for drug sensitivity evaluations.Methods:Tumor and adjacent tissues from female breast cancer patients were collected during surgery.Patient-derived breast cancer cells were cultured using the conditional reprogramming technique to establish 2D models.The obtained patient-derived conditional reprogramming breast cancer(CRBC)cells were subsequently embedded in alginate-gelatin methacryloyl hydrogel microspheres to form 3D culture models.Comparisons between 2D and 3D models were made using immunohistochemistry(tumor markers),MTS assays(cell viability),flow cytometry(apoptosis),transwell assays(migration),and Western blotting(protein expression).Drug sensitivity tests were conducted to evaluate patient-specific responses to anti-cancer agents.Results:2D and 3D culture models were successfully established using samples from eight patients.The 3D models retained histological and marker characteristics of the original tumors.Compared to 2D cultures,3D models exhibited increased apoptosis,enhanced drug resistance,elevated stem cell marker expression,and greater migration ability—features more reflective of in vivo tumor behavior.Conclusion:Patient-derived 3D CRBC models effectively mimic the in vivo tumor microenvironment and demonstrate stronger resistance to anti-cancer drugs than 2D models.These hydrogel-based models offer a cost-effective and clinically relevant platform for drug screening and personalized breast cancer treatment.
基金Supported by National Natural Science Foundation of China(Grant No.55275227)。
文摘Lead zirconate titanate(PbZr_(x)Ti_(1-x)O_(3),PZT)ferroelectric films possess remarkable characteristics such as high residual polarization,high dielectric constant,and high piezoelectric coefficient and have great application prospects in modern electronics,communications,medical care,and military fields.At present,the microstructure changes of PZT ferroelectric thin films have a significant impact on their electrical properties.Therefore,this work summarizes the influences of geometric structure(thickness,porosity),composition structure(Zr/Ti ratio,doping),and grain structure(grain size,grain boundaries,orientation)on the electrical properties of PZT ferroelectric thin films.The results show that the changes in thickness and porosity have a significant impact on the electrical properties of PZT ferroelectric films.Especially,the actual application scenarios and preparation processes determine the required geometric dimensions and structures of PZT ferroelectric films.The Zr/Ti ratio and doping mainly affect the electrical properties by influencing the phase composition of PZT ferroelectric films.The changes in grain size,boundary structure,and orientation dependence mainly have a certain degree of influence on the domain response and domain switching behavior of PZT ferroelectric thin films.In conclusion,different structures have different influence effects on the dielectric,ferroelectric,and piezoelectric properties of PZT ferroelectric films.The way the tiny structure affects how PZT thin films work was shown,helping to guide the design of ferroelectric thin film devices.In order to further study and apply piezoelectric ceramic devices,it is crucial to have an in-depth understanding of the relationship between the structure and performance of piezoelectric ceramic devices.
基金financially supported by the National Natural Science Foundation of China (Grant No.81473162)the Program for Liaoning Excellent Talents in Universitythe Young and middle-aged career development planning of Shenyang Pharmaceutical University
文摘Mitochondria are a novel and promising therapeutic target for diagnosis, treatment and prevention of a lot of human diseases such as cancer, metabolic diseases and neurodegenerative disease. Owing to the mitochondrial special bilayer structure and highly negative potential nature, therapeutic molecules have multiple difficulties in reaching mitochondria. To overcome multiple barriers for targeting mitochondria, the researchers developed various pharmaceutical preparations such as liposomes, polymeric nanoparticles and inorganic nanoparticles modified by mitochondriotropic moieties like dequalinium (DQA),triphenylphosphonium (TPP), mitochondrial penetrating peptides (MPPs) and mitochondrial protein import machinery that allow specific targeting.The targeted formulations exhibited enhanced pharmacological effect and better therapeutic effect than their untargeted counterpart both in vitro and in vivo. Nanocarriers may be used for bio-therapeutic delivery into specific mitochondria that possess a great potential treatment of mitochondria related diseases.
基金supported by National Natural Science Foundation of China(31972086,32172173,32072197)Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province。
文摘Blautia has attracted attention because of its potential efficacy in ameliorating host energy metabolism and inflammation.This study aims to investigate the influences of Blautia producta D4 on colitis induced by dextran sulfate sodium(DSS)and to reveal the underlying mechanisms.Results showed that B.producta D4 intervention significantly relieved body weight loss,and suppressed the elevation of pro-inflammatory cytokines(including interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β))and excessive oxidative stress(myeloperoxidease(MPO)activity,superoxide dismutase(SOD)activity,glutathione peroxidase(GSH-Px)activity,and malondialdehyde(MDA)level)in colitis mice.Moreover,the concentrations of tight junction proteins(occludin,claudin-1,and ZO-1)related to the intestinal barrier were obviously elevated,and colitis-related TLR4/NF-κB pathway activation was remarkably inhibited after B.producta D4 intervention.The intestinal microbial disorder was evidently ameliorated by increasing the relative abundance of Clostridium sensu stricto 1,Bifidobacterium,GCA-900066225,Enterorhabdus,and reducing the relative abundance of Lachnospiraceae NK4A136 group.In conclusion,oral administration of B.producta D4 could ameliorate DSS-induced colitis by suppressing inflammatory responses,maintaining the intestinal barrier,inhibiting TLR4/NF-κB pathway,and regulating intestinal microbiota balance.These results are conducive to accelerate the development of B.producta D4 as a functional probiotic for colitis.
基金financially supported by National Natural Science Foundation of China (31601466)funding from Outstanding Talent of “Qishan Scholar” of Fuzhou University (GXRC21049)Outstanding Young Scientific Talents of Fujian Agriculture and Forestry University (XJQ201607)。
文摘Levilactobacillus brevis FZU0713, a potential probiotic previously isolated from the traditional brewing process of Hongqu rice wine, may have the beneficial effects on improving lipid metabolism. This study aimed to evaluate the in vivo protective effects and possible mechanism of L. brevis FZU0713 on the disturbance of lipid metabolism in hyperlipidemic rats fed a high-fat diet(HFD). Results showed that oral administration of L. brevis FZU0713 could significantly inhibit obesity, ameliorate the lipid metabolism disorder, including serum/liver biochemical parameters and hepatic oxidative stress in HFD-fed rats. Histopathological result also indicated that dietary intervention of L. brevis FZU0713 could reduce the accumulation of lipid droplets in liver induced by 8 weeks HFD feeding. Furthermore, L. brevis FZU0713 intervention significantly increased the fecal levels of short-chain fatty acids(SCFAs, including acetate, propionate, butyrate, isobutyrate, valerate and isovalerate)in HFD-fed rats, which may be closely related to the changes of intestinal microbial composition and metabolic function. Intestinal microbiota profiling by 16S rRNA gene sequencing revealed that L. brevis FZU0713 intervention significantly altered the relative abundance of Coprococcus, Butyricicoccus, Intestinimonas, Lachnospiraceae FCS020 group, Ruminococcaceae_NK4A214 group, Ruminococcaceae_UCG-005 and UCG-014 at genus levels. Based on Spearman's rank correlation coefficient, serum and liver lipid metabolism related biochemical parameters were positively correlated with genera Ruminococcus, Pediococcus and Lachnospiraceae, but negatively correlated with genera Pseudoflavonifractor, Butyricicoccus and Intestinimonas. Furthermore, liver metabolomics analysis demonstrated that L. brevis FZU0713 had a significant regulatory effect on the composition of liver metabolites in hyperlipidemic rats, especially the levels of some important biomarkers involved in the metabolic pathways of arachidonic acid metabolism, primary bile acid biosynthesis, amino sugar and nucleotide sugar metabolism, taurine and hypotaurine metabolism, biosynthesis of unsaturated fatty acid, fructose and mannose metabolism, tyrosine metabolism, etc. Additionally, oral administration of L. brevis FZU0713 significantly regulated the mR NA levels of liver genes(including Acat2, Acox1, Hmgcr, Cd36, Srebp-1c and Cyp7a1)involved in lipid metabolism and bile acid homeostasis. In conclusion, our findings provide the evidence that L. brevis FZU0713 has the potential to improve disturbance of lipid metabolism by regulating intestinal microflora and liver metabonomic profile. Therefore, L. brevis FZU0713 may be used as a potential probiotic strain to produce functional food to prevent hyperlipidemia.
基金Supported by National Natural Science Foundation of China(Grant Nos.52275227,52130509)Key Basic Research Project of the Foundation Strengthening Plan of China(Grant Nos.2019-JCJQ-JJ-034,2019-JCJQ-ZD-302).
文摘Photonic crystals are periodic structural materials that have an impact on the propagation properties of photons.Due to their excellent optical,electrical and magnetic properties,their advantages and potential for applications in the above areas are gradually emerging.Therefore,an increasing number of researchers have focused on photonic crystals.In this paper,the characteristics of biological photonic crystal structures,such as those found in butterfly wings,sea mouse bristles,peacock feathers,melon jellyfish epidermal cells,and weevil exoskeletons,are described.The preparation methods of photonic crystals are systematically summarized(including the template method,self-assembly technology,electron beam evaporation coating technology,chemical vapor deposition technology,femtosecond laser two-photon technology,spin coating technology,and a variety of technology mixing),and the characteristics,advantages,and disadvantages of the different methods are compared.Furthermore,the development of photonic crystals in the field of sensors,solar cells,filters,and infrared stealth is discussed,demonstrateing the great development potential of photonic crystals.It is concluded that the realization of photonic crystals with high precision,high sensitivity,angle independence,and large-area uniform preparation is a key problem requiring urgent solution.Moreover,photonic crystals have potential development prospects in the fields of equipment stealth,new concept weapons,production,an daily life.
基金funded by Outstanding Talent of“Qishan Scholar”of Fuzhou University of China(GXRC21049)the Open Project Program of the Beijing Laboratory of Food Quality and Safety,Beijing Technology and Business University(BTBU)(FQS-201802,FQS-202008).
文摘This study aimed to investigate microbial succession and metabolic dynamics during the traditional fermentation of Hongqu aged vinegar,and explore the core functional microbes closely related to the formation of flavor components.Microbiome analysis demonstrated that Lactobacillus,Acetobacter,Bacillus,Enterobacter,Lactococcus,Leuconostoc and Weissella were the predominant bacterial genera,while Aspergillus piperis,Aspergillus oryzae,Monascus purpureus,Candida athensensis,C.xylopsoci,Penicillium ochrosalmoneum and Simplicillium aogashimaense were the predominant fungal species.Correlation analysis revealed that Acetobacter was positively correlated with the production of tetramethylpyrazine,acetoin and acetic acid,Lactococcus showed positive correlation with the production of 2-nonanone,2-heptanone,ethyl caprylate,ethyl caprate,1-hexanol,1-octanol and 1-octen-3-ol,C.xylopsoci and C.rugosa were positively associated with the production of diethyl malonate,2,3-butanediyl diacetate,acetoin,benzaldehyde and tetramethylpyrazine.Correspondingly,non-volatile metabolites were also detected through ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry.A variety of amino acids and functional dipeptides were identified during the traditional brewing of Hongqu aged vinegar.Correlation analysis revealed that Lactobacillus was significantly associated with DL-lactate,indolelactic acid,D-(+)-3-phenyllactic acid,pimelic acid,pregabalin and 3-aminobutanoic acid.This study is useful for understanding flavor formation mechanism and developing effective strategies for the suitable strains selection to improve the flavor quality of Hongqu aged vinegar.
基金supported by National Natural Science Foundation of China(Grant No.31972086,32172173,32072197)Yongjiang Talent Introduction Programme(2021C-003-T)Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province.
文摘Barley beverage contains many natural nutrients which is suitable for lactic acid bacteria(LAB)fermentation.The aim of this study was to explore the influences of Lacticaseibacillus casei 17301 fermentation on the bioactive ingredients,volatile and non-volatile compounds,and physiological properties of barley beverages.Our results showed that the total polyphenols and flavonoids in fermented barley beverages remarkably increased compared to those in unfermented barley beverages.A total of 63 volatile compounds in fermented barley beverages at the different fermentation stages were detected using headspace solid-phase micro-extraction coupled with gas chromatography-mass spectrometry(HS-SPME-GC-MS),including 17 acids,16 ketones,11 aldehydes,and 7 alcohols.Furthermore,untargeted metabolomics analysis-based ultra-performance liquid chromatography quadrupole-time of flight mass spectrometry(UHPLC-QTOF-MS)revealed L.casei 17301 effectively altered the non-volatile compounds,especially indole-3-lactic acid,in barley beverages.Most importantly,the barley beverage fermented with L.casei 17301 had elevated the antioxidant and lipase inhibition activities compared to the unfermented barley beverage.Collectively,L.casei 17301 could ameliorated the functionality and flavor of the barley beverage and the flavor of the product.These results of this study provide a theoretical basis for the development of Lacticaseibacillus-fermented cereal products with beneficial physiological properties.
基金funded by the Research Project of Fuzhou University(XRC-25053)Natural Science Foundation of Fujian Province(2025J011544)Collaborative Innovation Platform Project of Fuzhou-Xiamen-Quanzhou National Independent Innovation Demonstration Zone(2024-P-001).
文摘Acute liver injury(ALI)is one of the most prevalent liver diseases,which have become a major health threat worldwide.Here,the meaning of this research is to explore the ability of L.rhamnosus FJG1530 to alleviate ALI in mice.In this study,L.rhamnosus FJG1530 alleviated lipopolysaccharide(LPS)-induced ALI symptoms,including elevated transaminase(aspartate aminotransferase[AST],and alanine aminotransferase[ALT])levels,liver tissue damage,increased liver oxidative stress(superoxide dismutase[SOD],catalase[CAT],glutathione[GSH],and total antioxidant capacity[T-AOC]),and high concentration of inflammatory cytokines(tumor necrosis factor-α[TNF-α],interleukin-1β[IL-1β],and interleukin-6[IL-6]).Additionally,the mice treated with L.rhamnosus FJG1530 showed an upward trend in short-chain fatty acid content in the cecum,and alteration of the gut microbiota structure.The study also discovered that L.rhamnosus FJG1530 intervention improved the composition of liver metabolites,and these characteristic biomarkers mainly involving galactose metabolism,histidine metabolism,ascorbate and aldarate metabolism etc.In summary,L.rhamnosus FJG1530 has the ability to prevent the occurrence of ALI through impairing the inflammatory response,curbing liver oxidative stress,and adjusting the gut microbiota structure.
