Infectious wound healing is complicated with and limited by infection and oxidative stress at the wound site.In recent years,various evidences suggest that nanozymes with multiple enzymatic activities have enabled the...Infectious wound healing is complicated with and limited by infection and oxidative stress at the wound site.In recent years,various evidences suggest that nanozymes with multiple enzymatic activities have enabled the development of novel strategies for infectious wound healing.In this study,epigallocatechin gallate loaded polydopamine(P@E)was developed to act as a potent reactive oxygen species(ROS)scavenger for scavenging ROS,alleviating inflammatory responses,and promoting infectious wound healing.Combining with near infrared(NIR)irradiation,P@E presented excellent antibacterial ability of Escherichia coli(E.coli,93.6%)and methicillin-resistant Staphylococcus aureus(MRSA,87.6%).Specifically,P@E+NIR exhibited the most potent antioxidant,anti-inflammatory and cell proliferation behaviors through downregulating intracellular ROS levels(81.9%and 94.3%for NIH3T3 and RAW264.7 respectively)and inducible nitric oxide synthase(iNOS)expression level(55.7%),and up-regulating the expression levels of arginase-1(Arg-1,71.4%),heat shock protein 70(HSP70,48.6%)and platelet endothelial cell adhesion molecule(CD31,35.3%)compared to control group.Meanwhile,it also efficiently induced M2 directional polarization of lipopolysaccharide induced murine macrophages to achieve anti-inflammation,indicated by the down-regulation of CD86(86.2%),and up-regulation of CD206(85.6%).Significantly,it was also observed that P@E+NIR presented the excellent behaviors of inhibiting wound infection,alleviating wound inflammation,as well as promoting skin tissue repairing.Altogether,it has developed the strategy of using P@E combining with NIR irradiation for the synergistic enhanced healing of infectious skin wound,which can serve as a promising therapeutic strategy for its clinical treatment.展开更多
基金financially supported by the Natural Science Foundation of Guangxi(Nos.2022GXNSFAA035505 and 2024GXNSFAA010164)the Key Research&Development Program of Guangxi(No.GuiKe2024AB08057)+1 种基金the Guangxi Medical and Health Suitable Technology Development and Popularization Applications Project(Nos.S2023093 and S2021084)the Major Talent Project of Guangxi Autonomous Region.
文摘Infectious wound healing is complicated with and limited by infection and oxidative stress at the wound site.In recent years,various evidences suggest that nanozymes with multiple enzymatic activities have enabled the development of novel strategies for infectious wound healing.In this study,epigallocatechin gallate loaded polydopamine(P@E)was developed to act as a potent reactive oxygen species(ROS)scavenger for scavenging ROS,alleviating inflammatory responses,and promoting infectious wound healing.Combining with near infrared(NIR)irradiation,P@E presented excellent antibacterial ability of Escherichia coli(E.coli,93.6%)and methicillin-resistant Staphylococcus aureus(MRSA,87.6%).Specifically,P@E+NIR exhibited the most potent antioxidant,anti-inflammatory and cell proliferation behaviors through downregulating intracellular ROS levels(81.9%and 94.3%for NIH3T3 and RAW264.7 respectively)and inducible nitric oxide synthase(iNOS)expression level(55.7%),and up-regulating the expression levels of arginase-1(Arg-1,71.4%),heat shock protein 70(HSP70,48.6%)and platelet endothelial cell adhesion molecule(CD31,35.3%)compared to control group.Meanwhile,it also efficiently induced M2 directional polarization of lipopolysaccharide induced murine macrophages to achieve anti-inflammation,indicated by the down-regulation of CD86(86.2%),and up-regulation of CD206(85.6%).Significantly,it was also observed that P@E+NIR presented the excellent behaviors of inhibiting wound infection,alleviating wound inflammation,as well as promoting skin tissue repairing.Altogether,it has developed the strategy of using P@E combining with NIR irradiation for the synergistic enhanced healing of infectious skin wound,which can serve as a promising therapeutic strategy for its clinical treatment.
