The Hedgehog(Hh) signalling pathway is essential for cellular proliferation and differentiation during embryonic development.Gain and loss of function of Hh signalling are known to result in an array of craniofacial m...The Hedgehog(Hh) signalling pathway is essential for cellular proliferation and differentiation during embryonic development.Gain and loss of function of Hh signalling are known to result in an array of craniofacial malformations. To determine the critical period for Hh pathway antagonist-induced frontal bone hypoplasia, we examined patterns of dysmorphology caused by Hh signalling inhibition. Pregnant mice received a single oral administration of Hh signalling inhibitor GDC-0449 at 100 or 150 mg·kg^(-1) body weight at preselected time points between embryonic days(E)8.5 and 12.5. The optimal teratogenic concentration of GDC-0449 was determined to be 150 mg·kg^(-1). Exposure between E9.5 and E10.5 induced frontal bone dysplasia, micrognathia and limb defects, with administration at E10.5 producing the most pronounced effects. This model showed decreased ossification of the frontal bone with downregulation of Hh signalling. The osteoid thickness of the frontal bone was significantly reduced. The amount of neural crest-derived frontal bone primordium was reduced after GDC-0449 exposure owing to a decreased rate of cell proliferation and increased cell death.展开更多
Comprehensively promoting curriculum ideological and political education is a strategic measure to implement the fundamental task of establishing moral integrity and educating people.Culinary raw materials is the core...Comprehensively promoting curriculum ideological and political education is a strategic measure to implement the fundamental task of establishing moral integrity and educating people.Culinary raw materials is the core curriculum of culinary majors,which is rich in ideological and political elements.In this study,taking livestock and poultry raw materials as an example,the teaching design of integrating curriculum ideological and political education into culinary raw materials were investigated by sorting out its teaching contents and excavating its ideological and political elements.Taking livestock and poultry raw materials in Teochew Cuisine Raw Materials as an example,the teaching design of curriculum ideological and political education was conducted,aiming to provide some reference and guidance for curriculum ideological and political education of the curriculum.展开更多
With the continuous deepening of higher education reform,curriculum ideological and political education has become a key path to achieving the fundamental task of cultivating morality and talents.In this paper,through...With the continuous deepening of higher education reform,curriculum ideological and political education has become a key path to achieving the fundamental task of cultivating morality and talents.In this paper,through a detailed review and analysis on the content of the textbook Teochew Cuisine Raw Materials Science,the ideological and political elements of the curriculum in the field of culinary raw materials are revealed,and multidimensional ideological and political elements such as craftsmanship spirit,cultural confidence,and professional ethics are summarized.These elements aim to guide students to establish correct values and worldviews,providing valuable theoretical support for curriculum teaching.展开更多
The progression of tumors is heavily influenced by mechanical properties of their microenvironment.In this work,we applied micropatterned models with varying distances and shapes to investigate the differences between...The progression of tumors is heavily influenced by mechanical properties of their microenvironment.In this work,we applied micropatterned models with varying distances and shapes to investigate the differences between metastatic MDA-MB-231 and non-metastatic MCF-7 breast cancer cells in reconfiguring extracellular matrix to promote cell migration induced by cell mechanics.Both cancer cells were able to rearrange type I collagen(COL)to form fibre threads,in which MDA-MB-231 consistently migrated more rapidly than MCF-7,ranging from geometrical square arrays with different spacings to complex polygonal models.MDA-MB-231 displayed higher capability of reorganizing fibre bundles at longer distance(800μm).Further looking for differences in cell molecular mechanisms,siRNA knockdown inhibiting either integrinβ1 or Piezo1 decreased fibre assembly and reduced the difference in COL remodeling and migration between two cancer cells.MDA-MB-231 showed inhibited migration with integrin knockdown,whereas scattering migration with Piezo1 knockdown,indicating cells losing directional mechanosensation.After inhibiting junctional E-cadherin with siRNA,MCF-7 cells migrated faster,resulting in reduced difference in comparison to MDA-MB-231 that didn't express E-cadherin.In summary,this work has explored the biomechanical differences between metastatic and non-metastatic breast cancer cells regarding COL fibre matrix remodeling and cell movements.The significant differences in E-cadherin expression in the two breast cancer cells had an effect on cell migrations.The results of this study provide research approaches for evaluating therapeutic effort on breast cancer.展开更多
Living tissues often have anisotropic and heterogeneous organizations, in which developmental processes are coordinated by cells and extracellular matrix modeling. Cells have the capability of modeling matrix in long ...Living tissues often have anisotropic and heterogeneous organizations, in which developmental processes are coordinated by cells and extracellular matrix modeling. Cells have the capability of modeling matrix in long distance;however, the biophysical mechanism is largely unknown. We investigated the dynamic remodeling of collagen I (COL) fibril matrix by cell contraction with designed patterns of cell clusters. By considering cell dynamic contractions, our molecular dynamics simulations predicted the anisotropic patterns of the observed COL bundling in experiments with various geometrical patterns without spatial limitation. The pattern of COL bundling was closely related to the dynamic remodeling of fibril under cell active contraction. We showed that cell cytoskeletal integrity (actin filaments and microtubules), actomyosin contractions, and endoplasmic reticulum calcium channels acting as force generations and transductions were essential for fiber bundling inductions, and membrane mechanosensory components integrin and Piezo played critical roles as well. This study revealed the underlying mechanisms of the cell mechanics-induced matrix remodeling in large scales and the associated cellular mechanism and should provide important guidelines for tissue engineering in potential biomedical applications.展开更多
基金supported by the National Natural Science Foundation of China (31070838)
文摘The Hedgehog(Hh) signalling pathway is essential for cellular proliferation and differentiation during embryonic development.Gain and loss of function of Hh signalling are known to result in an array of craniofacial malformations. To determine the critical period for Hh pathway antagonist-induced frontal bone hypoplasia, we examined patterns of dysmorphology caused by Hh signalling inhibition. Pregnant mice received a single oral administration of Hh signalling inhibitor GDC-0449 at 100 or 150 mg·kg^(-1) body weight at preselected time points between embryonic days(E)8.5 and 12.5. The optimal teratogenic concentration of GDC-0449 was determined to be 150 mg·kg^(-1). Exposure between E9.5 and E10.5 induced frontal bone dysplasia, micrognathia and limb defects, with administration at E10.5 producing the most pronounced effects. This model showed decreased ossification of the frontal bone with downregulation of Hh signalling. The osteoid thickness of the frontal bone was significantly reduced. The amount of neural crest-derived frontal bone primordium was reduced after GDC-0449 exposure owing to a decreased rate of cell proliferation and increased cell death.
基金Supported by 2021 Continuing Education Teaching Reform and Research Practice Project of Continuing Education Quality Improvement Project in Guangdong Province(JXJYGC2021KY0667)2021 Online and Offline Mixed First-class Undergraduate Curriculum"Culinary Raw Materials"of Hanshan Normal University.
文摘Comprehensively promoting curriculum ideological and political education is a strategic measure to implement the fundamental task of establishing moral integrity and educating people.Culinary raw materials is the core curriculum of culinary majors,which is rich in ideological and political elements.In this study,taking livestock and poultry raw materials as an example,the teaching design of integrating curriculum ideological and political education into culinary raw materials were investigated by sorting out its teaching contents and excavating its ideological and political elements.Taking livestock and poultry raw materials in Teochew Cuisine Raw Materials as an example,the teaching design of curriculum ideological and political education was conducted,aiming to provide some reference and guidance for curriculum ideological and political education of the curriculum.
基金Supported by 2021 Continuing Education Teaching Reform and Research Practice Project of Continuing Education Quality Improvement Project in Guangdong Province(JXJYGC2021KY0667)2021 Online and Offline Mixed First-class Undergraduate Curriculum"Culinary Raw Materials"of Hanshan Normal University.
文摘With the continuous deepening of higher education reform,curriculum ideological and political education has become a key path to achieving the fundamental task of cultivating morality and talents.In this paper,through a detailed review and analysis on the content of the textbook Teochew Cuisine Raw Materials Science,the ideological and political elements of the curriculum in the field of culinary raw materials are revealed,and multidimensional ideological and political elements such as craftsmanship spirit,cultural confidence,and professional ethics are summarized.These elements aim to guide students to establish correct values and worldviews,providing valuable theoretical support for curriculum teaching.
基金supported financially by National Natural Science Foundation of China(NSFC12372312,11872129)Projects of“Jiangsu Specially-appointed Professor”(M.O.)National Natural Science Foundation of China(11902051(B.B.),12272063(L.D.)).
文摘The progression of tumors is heavily influenced by mechanical properties of their microenvironment.In this work,we applied micropatterned models with varying distances and shapes to investigate the differences between metastatic MDA-MB-231 and non-metastatic MCF-7 breast cancer cells in reconfiguring extracellular matrix to promote cell migration induced by cell mechanics.Both cancer cells were able to rearrange type I collagen(COL)to form fibre threads,in which MDA-MB-231 consistently migrated more rapidly than MCF-7,ranging from geometrical square arrays with different spacings to complex polygonal models.MDA-MB-231 displayed higher capability of reorganizing fibre bundles at longer distance(800μm).Further looking for differences in cell molecular mechanisms,siRNA knockdown inhibiting either integrinβ1 or Piezo1 decreased fibre assembly and reduced the difference in COL remodeling and migration between two cancer cells.MDA-MB-231 showed inhibited migration with integrin knockdown,whereas scattering migration with Piezo1 knockdown,indicating cells losing directional mechanosensation.After inhibiting junctional E-cadherin with siRNA,MCF-7 cells migrated faster,resulting in reduced difference in comparison to MDA-MB-231 that didn't express E-cadherin.In summary,this work has explored the biomechanical differences between metastatic and non-metastatic breast cancer cells regarding COL fibre matrix remodeling and cell movements.The significant differences in E-cadherin expression in the two breast cancer cells had an effect on cell migrations.The results of this study provide research approaches for evaluating therapeutic effort on breast cancer.
基金the National Natural Science Foundation of China(NSFC 11872129 and 12372312)the Natural Science Foundation of Jiangsu Province(BK20181416)+3 种基金Projects of“Jiangsu Specially-appointed Professor”and“Jiangsu six talent peaks(C)”(M.O.)the National Natural Science Foundation of China(11902051)(B.B.)the National Natural Science Foundation of China(11532003)(L.D.)the National Natural Science Foundation of China(11932017)(B.J.).
文摘Living tissues often have anisotropic and heterogeneous organizations, in which developmental processes are coordinated by cells and extracellular matrix modeling. Cells have the capability of modeling matrix in long distance;however, the biophysical mechanism is largely unknown. We investigated the dynamic remodeling of collagen I (COL) fibril matrix by cell contraction with designed patterns of cell clusters. By considering cell dynamic contractions, our molecular dynamics simulations predicted the anisotropic patterns of the observed COL bundling in experiments with various geometrical patterns without spatial limitation. The pattern of COL bundling was closely related to the dynamic remodeling of fibril under cell active contraction. We showed that cell cytoskeletal integrity (actin filaments and microtubules), actomyosin contractions, and endoplasmic reticulum calcium channels acting as force generations and transductions were essential for fiber bundling inductions, and membrane mechanosensory components integrin and Piezo played critical roles as well. This study revealed the underlying mechanisms of the cell mechanics-induced matrix remodeling in large scales and the associated cellular mechanism and should provide important guidelines for tissue engineering in potential biomedical applications.
