The problem of cavity stability widely exists in deep underground engineering and energy exploitation.First,the stress field of the surrounding rock under the uniform stress field is deduced based on a post-peak stren...The problem of cavity stability widely exists in deep underground engineering and energy exploitation.First,the stress field of the surrounding rock under the uniform stress field is deduced based on a post-peak strength drop model considering the rock’s characteristics of constant modulus and double moduli.Then,the orthogonal non-associative flow rule is used to establish the displacement of the surrounding rock under constant modulus and double moduli,respectively,considering the stiffness degradation and dilatancy effects in the plastic region and assuming that the elastic strain in the plastic region satisfies the elastic constitutive relationship.Finally,the evolution of the displacement in the surrounding rock is analyzed under the effects of the double modulus characteristics,the strength drop,the stiffness degradation,and the dilatancy.The results show that the displacement solutions of the surrounding rock under constant modulus and double moduli have a unified expression.The coefficients of the expression are related to the stress field of the original rock,the elastic constant of the surrounding rock,the strength parameters,and the dilatancy angle.The strength drop,the stiffness degradation,and the dilatancy effects all have effects on the displacement.The effects can be characterized by quantitative relationships.展开更多
A new quantitative determination method of proteins using beryllon ?by voltammetric technique was developed in this paper. In pH 3.5 Britton-Robinson (B-R) buffer solution, beryllon ?can bind with human serum albumin...A new quantitative determination method of proteins using beryllon ?by voltammetric technique was developed in this paper. In pH 3.5 Britton-Robinson (B-R) buffer solution, beryllon ?can bind with human serum albumin (HSA) to form an electro-inactive supermolecular complex. Beryllon ?has a well-defined voltammetric reduction peak at 0.38 V (vs. SCE) and the addition of protein in this solution can cause the decrease of the reductive peak current. Based on the decrease of the reduction peak current, a new electrochemical method for the determination of HSA was established with linear range of 1.0~40.0 mg/L and the detection limit of 1.0 mg/L. This method is further applied to the determination of real sample of healthy human serum.展开更多
Objective To estimate the effect of two simple filters, two or more positive peptide filter and Unified Score filter on the true positive rate of protein and peptide. Methods Twenty-two LC-MS/MS datasets were from 18 ...Objective To estimate the effect of two simple filters, two or more positive peptide filter and Unified Score filter on the true positive rate of protein and peptide. Methods Twenty-two LC-MS/MS datasets were from 18 known protein mixture. Two or more positive peptide filter and Unified Score filter were applied to the 22 datasets. The filters effect was evaluated according to the true positive rate of protein and peptide for each filter. Results The positive rates of protein and peptide from two or more peptide filter raised from 56.49% to 92.86%-99.12% (for protein) and from 90.67% to 97.74%-99.62% (for peptide), but many positive proteins were filtered out. The positive rates of protein and peptide from Unified Score (ThermoFinnigan value 2400) were only about 35.51% and 82.99%, but after adjusted the value (3900) according to the number of false positive peptide, those positive rate raised to 63.61% (for protein) and 91.97% (for peptide). Conclusions Two or more peptides requirement could significantly decrease false positive rate, but it also may filter out many true positive proteins especially low molecular weight and less abundant proteins. Unified Score may be a better filter than Xcorr and DeltaCn combination and the value of 3900 is found to be more suitable for this particular datasets.展开更多
基金Project supported by the National Natural Science Foundation of China and Shandong Province Joint Program(No.U1806209)the National Natural Science Foundation of China(Nos.51774196 and 51774194)and Shandong University of Science and Technology(SDUST)Research Fund(No.2019TDJH101)。
文摘The problem of cavity stability widely exists in deep underground engineering and energy exploitation.First,the stress field of the surrounding rock under the uniform stress field is deduced based on a post-peak strength drop model considering the rock’s characteristics of constant modulus and double moduli.Then,the orthogonal non-associative flow rule is used to establish the displacement of the surrounding rock under constant modulus and double moduli,respectively,considering the stiffness degradation and dilatancy effects in the plastic region and assuming that the elastic strain in the plastic region satisfies the elastic constitutive relationship.Finally,the evolution of the displacement in the surrounding rock is analyzed under the effects of the double modulus characteristics,the strength drop,the stiffness degradation,and the dilatancy.The results show that the displacement solutions of the surrounding rock under constant modulus and double moduli have a unified expression.The coefficients of the expression are related to the stress field of the original rock,the elastic constant of the surrounding rock,the strength parameters,and the dilatancy angle.The strength drop,the stiffness degradation,and the dilatancy effects all have effects on the displacement.The effects can be characterized by quantitative relationships.
基金supported by the National Natural Science Foundation of China(Grant No.20075013)the Younger Foundation of Qingdao University of Science and Technology(Grant No.22000219).
文摘A new quantitative determination method of proteins using beryllon ?by voltammetric technique was developed in this paper. In pH 3.5 Britton-Robinson (B-R) buffer solution, beryllon ?can bind with human serum albumin (HSA) to form an electro-inactive supermolecular complex. Beryllon ?has a well-defined voltammetric reduction peak at 0.38 V (vs. SCE) and the addition of protein in this solution can cause the decrease of the reductive peak current. Based on the decrease of the reduction peak current, a new electrochemical method for the determination of HSA was established with linear range of 1.0~40.0 mg/L and the detection limit of 1.0 mg/L. This method is further applied to the determination of real sample of healthy human serum.
基金Supported by grants from the National Basic Research Program (973)2004CB520804 ,National Natural Science Foundation of China (30270657,30230150 ,3037030 ).
文摘Objective To estimate the effect of two simple filters, two or more positive peptide filter and Unified Score filter on the true positive rate of protein and peptide. Methods Twenty-two LC-MS/MS datasets were from 18 known protein mixture. Two or more positive peptide filter and Unified Score filter were applied to the 22 datasets. The filters effect was evaluated according to the true positive rate of protein and peptide for each filter. Results The positive rates of protein and peptide from two or more peptide filter raised from 56.49% to 92.86%-99.12% (for protein) and from 90.67% to 97.74%-99.62% (for peptide), but many positive proteins were filtered out. The positive rates of protein and peptide from Unified Score (ThermoFinnigan value 2400) were only about 35.51% and 82.99%, but after adjusted the value (3900) according to the number of false positive peptide, those positive rate raised to 63.61% (for protein) and 91.97% (for peptide). Conclusions Two or more peptides requirement could significantly decrease false positive rate, but it also may filter out many true positive proteins especially low molecular weight and less abundant proteins. Unified Score may be a better filter than Xcorr and DeltaCn combination and the value of 3900 is found to be more suitable for this particular datasets.
