AIM: To investigate the inhibitory effects of endostatinvascular endothelial growth inhibitor (VEGI151) recombinant adenoviruses on neovascularization.METHODS: We used recombinant adenoviruses to treat human vascular ...AIM: To investigate the inhibitory effects of endostatinvascular endothelial growth inhibitor (VEGI151) recombinant adenoviruses on neovascularization.METHODS: We used recombinant adenoviruses to treat human vascular endothelial cell line ECV304, human hepatocellular carcinoma cell line HepG2, and murine fibroblast cell line L929, in order to study the chimeric gene expression in these cell lines. Chick choriallantic membrane(CAM) model, rabbit inflammatory corneal neovascularization(CNV) model, and liver cancer-bearing nude mice model were employed to investigate the negative biological effect of fusion molecules on neovascularization in vivo.RESULTS: Western blot showed that the molecular weight of fusion protein was about 41kD after infection of ECV304,HepG2 and L929 cells with supernatant of AdhENDO-VEGI151.The fusion protein showed a specific inhibitory effect on the proliferation of ECV304 cells, but no inhibitory effect on the growth of HepG2 and L929 cells (F=13112.13, P=0.0001). In the chick choriallantic membrane (CAM) assay, the expressed fusion protein significantly inhibited neovascularization. Rabbit inflammatory corneal neovascularization (CNV) induced by intrastromal sutures resulted in a uniform neovascular response. In this model, direct subconjunctival injection of AdhENDO-VEGI151 expressed the fusion protein in vivo and suppressed the development of CNV. Topical application of AdhENDO-VEGI151 led to a significant suppression of CNV (F=1413.11, P=0.0001),as compared with the control group of AdLacZ. Immunohistochemical staining showed the fusion protein dominantly expressed in corneal epithelium. Compared with the control group of AdLacZ (4075.9±1849.9 mm^3), the average tumor size of group AdhENDO-VEGI151 reduced in size (487.7±241.2 mm^3) (F=14.80, P=0.0085), with an inhibition rate of 88.03%. Immunohistochemical staining showed the adenoviruses carried the fusion gene expressed on liver cancer cell membrane. MVD decreased more significantly in treated mice (30.75±3.31%) than in AdLacZ control(50.25±8.65%) (F=17.72, P=0.0056) with an inhibition rate of 39%.CONCLUSION: Fusion protein expressed by recombinant adenoviruses has a significant inhibitory effect on neovascularization.展开更多
Concrete containing short carbon-coated-nylon fibers (0.4-2.0 vol. pct) exhibited quasi-ductile response by developing a large damage zone prior to fracture localization. In the damage zone, the material was microcrac...Concrete containing short carbon-coated-nylon fibers (0.4-2.0 vol. pct) exhibited quasi-ductile response by developing a large damage zone prior to fracture localization. In the damage zone, the material was microcracked but continued to local strain-harden. The carbon-coated-nylon-fiber-reinforced concrete composites (NFRC) were found to be an intrinsically smart concrete that could sense elastic and inelastic deformation, as well as fracture. The fibers served to bridge the cracks and the carbon coating gave the conduction path. The signal provided came from the change in electrical resistance, which was reversible for elastic deformation and irreversible for inelastic deformation and fracture. The resistance decrease was due to the reduction of surface touch resistance between fiber and matrix and the crack closure. The resistance irreversible increase resulted from the crack opening and breakage of the carbon coating on nylon fiber.展开更多
基金Supported by The National Natural Science Foundation of China,No.30170891 and No.39970819
文摘AIM: To investigate the inhibitory effects of endostatinvascular endothelial growth inhibitor (VEGI151) recombinant adenoviruses on neovascularization.METHODS: We used recombinant adenoviruses to treat human vascular endothelial cell line ECV304, human hepatocellular carcinoma cell line HepG2, and murine fibroblast cell line L929, in order to study the chimeric gene expression in these cell lines. Chick choriallantic membrane(CAM) model, rabbit inflammatory corneal neovascularization(CNV) model, and liver cancer-bearing nude mice model were employed to investigate the negative biological effect of fusion molecules on neovascularization in vivo.RESULTS: Western blot showed that the molecular weight of fusion protein was about 41kD after infection of ECV304,HepG2 and L929 cells with supernatant of AdhENDO-VEGI151.The fusion protein showed a specific inhibitory effect on the proliferation of ECV304 cells, but no inhibitory effect on the growth of HepG2 and L929 cells (F=13112.13, P=0.0001). In the chick choriallantic membrane (CAM) assay, the expressed fusion protein significantly inhibited neovascularization. Rabbit inflammatory corneal neovascularization (CNV) induced by intrastromal sutures resulted in a uniform neovascular response. In this model, direct subconjunctival injection of AdhENDO-VEGI151 expressed the fusion protein in vivo and suppressed the development of CNV. Topical application of AdhENDO-VEGI151 led to a significant suppression of CNV (F=1413.11, P=0.0001),as compared with the control group of AdLacZ. Immunohistochemical staining showed the fusion protein dominantly expressed in corneal epithelium. Compared with the control group of AdLacZ (4075.9±1849.9 mm^3), the average tumor size of group AdhENDO-VEGI151 reduced in size (487.7±241.2 mm^3) (F=14.80, P=0.0085), with an inhibition rate of 88.03%. Immunohistochemical staining showed the adenoviruses carried the fusion gene expressed on liver cancer cell membrane. MVD decreased more significantly in treated mice (30.75±3.31%) than in AdLacZ control(50.25±8.65%) (F=17.72, P=0.0056) with an inhibition rate of 39%.CONCLUSION: Fusion protein expressed by recombinant adenoviruses has a significant inhibitory effect on neovascularization.
基金This work was supported by the National High Technology Research and Development Program of China under grant No.2002AA324060by the China Postdoctoral Science Foundation under grant 2003033139.
文摘Concrete containing short carbon-coated-nylon fibers (0.4-2.0 vol. pct) exhibited quasi-ductile response by developing a large damage zone prior to fracture localization. In the damage zone, the material was microcracked but continued to local strain-harden. The carbon-coated-nylon-fiber-reinforced concrete composites (NFRC) were found to be an intrinsically smart concrete that could sense elastic and inelastic deformation, as well as fracture. The fibers served to bridge the cracks and the carbon coating gave the conduction path. The signal provided came from the change in electrical resistance, which was reversible for elastic deformation and irreversible for inelastic deformation and fracture. The resistance decrease was due to the reduction of surface touch resistance between fiber and matrix and the crack closure. The resistance irreversible increase resulted from the crack opening and breakage of the carbon coating on nylon fiber.
