Pregnancy-associated breast cancer(PABC),including breast cancer during pregnancy(BCP)and postpartum breast cancer(PBC),has become a critical issue for clinicians in consideration of both maternal and offspring safety...Pregnancy-associated breast cancer(PABC),including breast cancer during pregnancy(BCP)and postpartum breast cancer(PBC),has become a critical issue for clinicians in consideration of both maternal and offspring safety during diagnosis and treatment.The recommendations in this guideline are mainly based on currently available high-quality prospective studies,retrospective cohort studies,meta-analyses,and guidelines and consensus documents published by internationally recognized or national societies or associations.Clinicians should carefully analyze the potential benefits and risks of each treatment before recommending whether to terminate the pregnancy or lactation,balance the advantages and disadvantages of the treatments,and respect the wishes of patients.展开更多
Innate lymphoid cells(ILCs)are a group of lymphocytes without diversified antigen receptors encoded by gene rearrangement on T and B cells.ILCs,which are tissue-resident innate immune cells,expressed particularly in t...Innate lymphoid cells(ILCs)are a group of lymphocytes without diversified antigen receptors encoded by gene rearrangement on T and B cells.ILCs,which are tissue-resident innate immune cells,expressed particularly in the mucosa or the barrier surface,contribute to the formation of lymphoid organs,the maintenance of tissue homeostasis,and the regulation of antimicrobial defenses.It has been recently reported that ILCs were enriched at the maternal-fetal interface.During a successful pregnancy,the maternal immune system must tolerate a fetus as an allograft.With the new defined of ILCs,a number of studies have shown that three types of ILCs are involved in embryonic development and pregnancy maintenance as well as the occurrence and development of pregnancy-related complications.This article reviews the types and roles of ILCs in normal pregnancy and pregnancy-related diseases.展开更多
Objective:Long non-coding RNAs(lncRNAs)are implicated in multiple pathophysiological processes in placenta-related disorders;however,their expression and function in late-onset pre-eclampsia(LOPE)remain unclear.This s...Objective:Long non-coding RNAs(lncRNAs)are implicated in multiple pathophysiological processes in placenta-related disorders;however,their expression and function in late-onset pre-eclampsia(LOPE)remain unclear.This study aimed to investigate the expression of lncRNAs in LOPE,construct a competing endogenous RNA(ceRNA)network,and identify the pathways associated with LOPE pathogenesis.Methods:We performed lncRNA and mRNAs microarray profiling to identify the differential expression profiles of lncRNAs and mRNAs in LOPE compared to those in normal pregnancy.Quantitative reverse transcription polymerase chain reaction(qRT-PCR)was performed to validate differentially expressed genes.Subsequently,we generated an interaction network between lncRNAs,(micro-RNAs)miRNAs,and mRNAs based on the Pearson’s correlation coefficient between lncRNAs and mRNAs.Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses were performed to understand the functional significance of differentially expressed lncRNAs(DElncRNAs)in LOPE.Results:We identified 29 DElncRNAs(25 upregulated and four downregulated)and 212 differentially expressed mRNAs(DEmRNAs;203 upregulated and nine downregulated)in LOPE placentas.Within them,six lncRNAs and four mRNAs were verified by qRT-PCR.GO and KEGG analyses revealed the potential pathways affected by these mRNAs,such as positive regulation of leukocyte chemotaxis,chemokine signaling pathway,and response to hypoxia.Finally,we constructed a ceRNA network including three DElncRNAs and 124 DEmRNAs,whose competing interactions may be mediated by 17 miRNAs.Two DElncRNAs,ENST00000515376 and ENST00000520544,were found to be hub genes,as they interacted with most miRNAs and mRNAs.ENST00000515376 is most likely related to the metabolic process of arachidonic acid,whereas ENST00000520544 is more likely related to the coagulation system,such as the regulation of blood coagulation and platelet degranulation.Conclusion:Differential expression profile of lncRNAs and the lncRNA-miRNA-mRNA network in LOPE provide potential therapeutic targets for this disease.展开更多
文摘Pregnancy-associated breast cancer(PABC),including breast cancer during pregnancy(BCP)and postpartum breast cancer(PBC),has become a critical issue for clinicians in consideration of both maternal and offspring safety during diagnosis and treatment.The recommendations in this guideline are mainly based on currently available high-quality prospective studies,retrospective cohort studies,meta-analyses,and guidelines and consensus documents published by internationally recognized or national societies or associations.Clinicians should carefully analyze the potential benefits and risks of each treatment before recommending whether to terminate the pregnancy or lactation,balance the advantages and disadvantages of the treatments,and respect the wishes of patients.
基金supported by grants from the National Natural Science Foundation of China(No.81801469 and 81971408).
文摘Innate lymphoid cells(ILCs)are a group of lymphocytes without diversified antigen receptors encoded by gene rearrangement on T and B cells.ILCs,which are tissue-resident innate immune cells,expressed particularly in the mucosa or the barrier surface,contribute to the formation of lymphoid organs,the maintenance of tissue homeostasis,and the regulation of antimicrobial defenses.It has been recently reported that ILCs were enriched at the maternal-fetal interface.During a successful pregnancy,the maternal immune system must tolerate a fetus as an allograft.With the new defined of ILCs,a number of studies have shown that three types of ILCs are involved in embryonic development and pregnancy maintenance as well as the occurrence and development of pregnancy-related complications.This article reviews the types and roles of ILCs in normal pregnancy and pregnancy-related diseases.
基金National Natural Sciences Foundation of China(81971408,81801469,81801468,and 81971411)National Key R&D Program of China(2016YFC1000403)2020"Diligence·Excellence"Clinical Innovative Team Project"Study on the comprehensive management of preeclampsia and its pathogenesis"conducted by Obstetrics and Gynecology Hospital of Fudan University(2021fckbc06)
文摘Objective:Long non-coding RNAs(lncRNAs)are implicated in multiple pathophysiological processes in placenta-related disorders;however,their expression and function in late-onset pre-eclampsia(LOPE)remain unclear.This study aimed to investigate the expression of lncRNAs in LOPE,construct a competing endogenous RNA(ceRNA)network,and identify the pathways associated with LOPE pathogenesis.Methods:We performed lncRNA and mRNAs microarray profiling to identify the differential expression profiles of lncRNAs and mRNAs in LOPE compared to those in normal pregnancy.Quantitative reverse transcription polymerase chain reaction(qRT-PCR)was performed to validate differentially expressed genes.Subsequently,we generated an interaction network between lncRNAs,(micro-RNAs)miRNAs,and mRNAs based on the Pearson’s correlation coefficient between lncRNAs and mRNAs.Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses were performed to understand the functional significance of differentially expressed lncRNAs(DElncRNAs)in LOPE.Results:We identified 29 DElncRNAs(25 upregulated and four downregulated)and 212 differentially expressed mRNAs(DEmRNAs;203 upregulated and nine downregulated)in LOPE placentas.Within them,six lncRNAs and four mRNAs were verified by qRT-PCR.GO and KEGG analyses revealed the potential pathways affected by these mRNAs,such as positive regulation of leukocyte chemotaxis,chemokine signaling pathway,and response to hypoxia.Finally,we constructed a ceRNA network including three DElncRNAs and 124 DEmRNAs,whose competing interactions may be mediated by 17 miRNAs.Two DElncRNAs,ENST00000515376 and ENST00000520544,were found to be hub genes,as they interacted with most miRNAs and mRNAs.ENST00000515376 is most likely related to the metabolic process of arachidonic acid,whereas ENST00000520544 is more likely related to the coagulation system,such as the regulation of blood coagulation and platelet degranulation.Conclusion:Differential expression profile of lncRNAs and the lncRNA-miRNA-mRNA network in LOPE provide potential therapeutic targets for this disease.
