Aim:To assess the effect of cyclosporine A(CyA)on the level of vascular endothelial growth factor(VEGF)in the plasma and urine of nephrotic syndrome children.Methods:The study material consisted of 15 children(F 6,M 9...Aim:To assess the effect of cyclosporine A(CyA)on the level of vascular endothelial growth factor(VEGF)in the plasma and urine of nephrotic syndrome children.Methods:The study material consisted of 15 children(F 6,M 9;group I)who were subjected to the following examinations:A)at the time of proteinuria relapse,before treatment with CyA,B)after 3 mo,C)after 6 mo,and D)after 12 mo of CyA administration with prednisone and convertase inhibitor.The control group(II)-contained 20 healthy children.The immunoenzymatic ELISA method(R&D Quantikine)was used to determine plasma and urinary VEGF levels,while the immunofluorescence method was applied to assess CyA concentration in the plasma.The statistical program Statistica 6.0 was used for statistical analysis of the results.Results:In the present study,plasma VEGF level in examination A was higher than in the control group(P < 0.01).After proteinuria regression(B),it did not differ from the level observed in healthy children(P > 0.05).After 6 and 12 mo of CyA administration,VEGF concentration increased and was higher than in the control group(P < 0.05).In all the examinations,urinary excretion of VEGF was higher than in the control group,increasing proportionally with the duration of treatment and plasma CyA level.A positive correlation was observed between plasma and urinary VEGF levels and between VEGF and CyA concentrations in the plasma.Conclusion:Long-term CyA treatment of nephrotic syndrome children leads to an increase in plasma and urinary VEGF.展开更多
Aim: The aim of the study was to assess plasma and urine concentrations of vascular endothelial growth factor (VEGF)in nephrotic syndrome children (NS) depending on the total dose of glucocorticoids (GC) and the perce...Aim: The aim of the study was to assess plasma and urine concentrations of vascular endothelial growth factor (VEGF)in nephrotic syndrome children (NS) depending on the total dose of glucocorticoids (GC) and the percentage of lymphocytes with glucocorticoid receptor expression (CD3/GCR). Methods: We examined 51 children (2- 15 years), allocated to three groups: group I: 13 children with the first NS onset, group II: 13 children with NS relapse, group C: 25 healthy children. The NS patients were examined: (A) before treatment and (B) 4- 5 weeks after prednisone administration at a dose of 60 mg/m 2/24 h. Plasma and urinary VEGF levels were determined using the immunoenzymatic ELISA method. Flow cytometry was applied to assess CD3/GCR expression. Results: Higher plasma and urinary VEGF concentrations were noted in NS children before treatment (A), as compared to control subjects (C). Following prednisone therapy (B), VEGF level was reduced but it was still higher than in the control group. Positive correlation was observed between VEGF and protein in the urine (group I r = 0.660, P < 0.05, group II r = 0.818, P < 0.01) and a weak positive correlation between VEGF in plasma and urine (group I r = 0.531, P < 0.05, group II - r = 0.581, P < 0.05). CD3/GCR expression was lower in group II. In both groups, the correlation between plasma VEGF and CD3/GCR was positive (P < 0.05). Conclusions: 1. Plasma and urinary VEGF levels increase during nephrotic syndrome onset. 2. Glucocorticoid treatment reduces plasma and urinary VEGF levels in NS children.展开更多
文摘Aim:To assess the effect of cyclosporine A(CyA)on the level of vascular endothelial growth factor(VEGF)in the plasma and urine of nephrotic syndrome children.Methods:The study material consisted of 15 children(F 6,M 9;group I)who were subjected to the following examinations:A)at the time of proteinuria relapse,before treatment with CyA,B)after 3 mo,C)after 6 mo,and D)after 12 mo of CyA administration with prednisone and convertase inhibitor.The control group(II)-contained 20 healthy children.The immunoenzymatic ELISA method(R&D Quantikine)was used to determine plasma and urinary VEGF levels,while the immunofluorescence method was applied to assess CyA concentration in the plasma.The statistical program Statistica 6.0 was used for statistical analysis of the results.Results:In the present study,plasma VEGF level in examination A was higher than in the control group(P < 0.01).After proteinuria regression(B),it did not differ from the level observed in healthy children(P > 0.05).After 6 and 12 mo of CyA administration,VEGF concentration increased and was higher than in the control group(P < 0.05).In all the examinations,urinary excretion of VEGF was higher than in the control group,increasing proportionally with the duration of treatment and plasma CyA level.A positive correlation was observed between plasma and urinary VEGF levels and between VEGF and CyA concentrations in the plasma.Conclusion:Long-term CyA treatment of nephrotic syndrome children leads to an increase in plasma and urinary VEGF.
文摘Aim: The aim of the study was to assess plasma and urine concentrations of vascular endothelial growth factor (VEGF)in nephrotic syndrome children (NS) depending on the total dose of glucocorticoids (GC) and the percentage of lymphocytes with glucocorticoid receptor expression (CD3/GCR). Methods: We examined 51 children (2- 15 years), allocated to three groups: group I: 13 children with the first NS onset, group II: 13 children with NS relapse, group C: 25 healthy children. The NS patients were examined: (A) before treatment and (B) 4- 5 weeks after prednisone administration at a dose of 60 mg/m 2/24 h. Plasma and urinary VEGF levels were determined using the immunoenzymatic ELISA method. Flow cytometry was applied to assess CD3/GCR expression. Results: Higher plasma and urinary VEGF concentrations were noted in NS children before treatment (A), as compared to control subjects (C). Following prednisone therapy (B), VEGF level was reduced but it was still higher than in the control group. Positive correlation was observed between VEGF and protein in the urine (group I r = 0.660, P < 0.05, group II r = 0.818, P < 0.01) and a weak positive correlation between VEGF in plasma and urine (group I r = 0.531, P < 0.05, group II - r = 0.581, P < 0.05). CD3/GCR expression was lower in group II. In both groups, the correlation between plasma VEGF and CD3/GCR was positive (P < 0.05). Conclusions: 1. Plasma and urinary VEGF levels increase during nephrotic syndrome onset. 2. Glucocorticoid treatment reduces plasma and urinary VEGF levels in NS children.
