InDel markers are commonly used to assess genetic relationships among populations.In this study,we employed a whole-genome sequence comparison method to identify and develop InDel markers for the rice blast fungus Pyr...InDel markers are commonly used to assess genetic relationships among populations.In this study,we employed a whole-genome sequence comparison method to identify and develop InDel markers for the rice blast fungus Pyricularia oryzae.We analyzed 152 whole-genome sequences of P.oryzae isolates from diverse global regions,including Brazil,Burundi,China,Colombia,C.te d’Ivoire,France,Ghana,Hungary,India,Japan,Korea,Laos,Madagascar,Mali,Morocco,Nepal,the Philippines,Portugal,Spain,Suriname,Thailand,the UK,the USA,and Zambia.Our analysis identified a total of 233,595 InDel loci distributed across the seven chromosomes of P.oryzae.From these,82 loci were selected based on their high polymorphism across the 152 genome sequences.The effectiveness of these 82 loci was assessed by analyzing the genetic diversity of 47 Thai rice blast isolates alongside two reference isolates,GUY11(France)and KJ201(Korea).Of the 82 InDel loci,33 exhibited polymorphisms,with 2-4 alleles per locus and polymorphic information content(PIC)scores ranging from 0.04 to 0.67.Principal coordinate and structure analyses revealed two genetic subgroups among the Thai rice blast isolates,categorized according to host specificity.Genetic relationships highlighted disparities among rice blast populations based on their respective hosts:rice and grassy weeds.This finding suggests a correlation between genetic relatedness and the plant hosts susceptible to rice blast disease.The newly developed InDel markers provide a valuable resource for future research in this field.展开更多
基金funded by the Graduate School Fellowship Program in Agriculture and Agro-industry from the Agricultural Research Development Agency(Public Organization)for the fiscal year 2022,the National Research Council of Thailand(NRCT),project no.N41A640140 and NRCT-RTA/812/2563,and Kasetsart UniversityNapassorn Thamkirati is financially supported by The Royal Golden Jubilee(RGJ)Ph.D.Program(Grant No.PHD/0138/2561),through the National Research Council of Thailand(NRCT),the Thailand Research Fund(TRF),and Kasetsart University.
文摘InDel markers are commonly used to assess genetic relationships among populations.In this study,we employed a whole-genome sequence comparison method to identify and develop InDel markers for the rice blast fungus Pyricularia oryzae.We analyzed 152 whole-genome sequences of P.oryzae isolates from diverse global regions,including Brazil,Burundi,China,Colombia,C.te d’Ivoire,France,Ghana,Hungary,India,Japan,Korea,Laos,Madagascar,Mali,Morocco,Nepal,the Philippines,Portugal,Spain,Suriname,Thailand,the UK,the USA,and Zambia.Our analysis identified a total of 233,595 InDel loci distributed across the seven chromosomes of P.oryzae.From these,82 loci were selected based on their high polymorphism across the 152 genome sequences.The effectiveness of these 82 loci was assessed by analyzing the genetic diversity of 47 Thai rice blast isolates alongside two reference isolates,GUY11(France)and KJ201(Korea).Of the 82 InDel loci,33 exhibited polymorphisms,with 2-4 alleles per locus and polymorphic information content(PIC)scores ranging from 0.04 to 0.67.Principal coordinate and structure analyses revealed two genetic subgroups among the Thai rice blast isolates,categorized according to host specificity.Genetic relationships highlighted disparities among rice blast populations based on their respective hosts:rice and grassy weeds.This finding suggests a correlation between genetic relatedness and the plant hosts susceptible to rice blast disease.The newly developed InDel markers provide a valuable resource for future research in this field.
