The oxidative dehydrogenation of propane to propylene using CO_(2)(CO_(2)-ODH)offers a promising route for both propylene production and CO_(2)utilization.In this study,we investigate the effect of alkali metal doping...The oxidative dehydrogenation of propane to propylene using CO_(2)(CO_(2)-ODH)offers a promising route for both propylene production and CO_(2)utilization.In this study,we investigate the effect of alkali metal doping on Pt-based catalysts in CO_(2)-ODH reactions.The optimized 0.1 KPt/S-1 catalyst achieved a high propane conversion of 48.3%,propylene selectivity of 85.5%,and CO_(2)conversion of 19.1%at a low temperature of 500℃with the Pt loading of 0.2 wt%and K loading of 0.1 wt%respectively.Characterization techniques,including high-resolution transmission electron microscope(HR-TEM),CO-diffuse reflectance infrared Fourier transform spectroscopy(CO-DRIFTS),X-ray absorption fine structure(XAFS),and X-ray Photoelectron Spectroscopy(XPS),revealed that the doping of K with Pt led to a strong interaction between potassium and platinum(Pt-KO_(x)cluster).This interaction resulted in a reduction of Pt particle size and a local enrichment of electron density around Pt atoms.These structural modifications improved the anchoring of Pt nanoparticles and enhanced Pt atom dispersion,thereby enhancing the activity of the catalyst and minimizing side reactions.Additionally,pyridine infrared(Py-IR)and temperature-programmed desorption(TPD)studies demonstrated that the prepared0.1 KPt/S-1 catalyst exhibited optimal acidity,which promoted C–H activation and facilitated the efficient adsorption and activation of CO_(2).These dual effects significantly lowered the activation energy for CO_(2)-ODH,enabling efficient dehydrogenation to propylene at a lower temperature of 500℃.This work highlights the critical role of alkali metal doping in modifying the electronic properties of Pt and optimizing catalyst acidity,which collectively contribute to the enhanced performance of the 0.1 KPt/S-1 catalyst.These findings offer valuable insights into the mechanistic pathway of CO_(2)-ODH and provide a foundation for the rational design of high-performance dehydrogenation catalysts.展开更多
The interface instability between composite solid electrolytes(CSEs)and lithium anode significantly shortens the lifespan of all-solid-state lithium batteries(ASSLBs)with high areal capacity.In this work,a CSE featuri...The interface instability between composite solid electrolytes(CSEs)and lithium anode significantly shortens the lifespan of all-solid-state lithium batteries(ASSLBs)with high areal capacity.In this work,a CSE featuring a trilayer architecture is developed by incorporating a thin polyethylene(PE)separator into a blending polymer matrix of poly(ethylene oxide)and poly(vinylidene fluoride)(PEO-PVDF)through a hot pressing technique.This structural design provides complementary functions:the flexible outer layers confine lithium deposition within a restricted area,while the robust interlayer prevents lithium dendrite penetration.Additionally,the incorporation of LiNO_(3) significantly enhances the stability of the CSE/Li interface by gradually forming a Li_(3)N-rich interfacial film,which promotes uniform lithium deposition.Consequently,the assembled Li||Li symmetrical cell demonstrates stable cycling for over 6000 h at a current density of 0.2 mA cm^(–2)with an areal capacity of 1.2 mAh cm^(–2).More attractively,ASSLBs constructed with the designed CSEs,high mass loading LFP/NCM811(LFP:LiFePO_(4);NCM811:LiNi_(0.8)Co_(0.1)Mn_(0.1)O_(2))cathodes(≥12 mg cm^(–2)),and lithium metal anodes deliver superior cycling performance without short-circuiting at current densities of 0.3/0.2 mA cm^(–2),respectively.This work offers critical insights for the design of high-performance ASSLBs with improved durability at high areal capacities.展开更多
Chronic heart failure(CHF)impairs cognitive function.Xijiaqi Formula(XJQ),a traditional Chinese medicine(TCM)used clinically to treat CHF,demonstrates potential for improving cognition in CHF patients.However,its prec...Chronic heart failure(CHF)impairs cognitive function.Xijiaqi Formula(XJQ),a traditional Chinese medicine(TCM)used clinically to treat CHF,demonstrates potential for improving cognition in CHF patients.However,its precise mechanism in treating post-CHF cognitive dysfunction remains unclear.This study systematically investigates XJQ’s effects on post-CHF cognitive dysfunction and the underlying mechanisms.The components of XJQ were identified through liquid chromatography-mass spectrometry.CHF was induced in rats via ligation of the left anterior descending coronary artery,followed by six weeks of XJQ treatment.Cardiac function was evaluated through echocardiography and hemodynamic parameters,while cognitive function was assessed using Morris water maze(MWM)and open field tests(OFT).XJQ treatment enhanced both cardiac and cognitive functions in CHF rats.Network pharmacology identified 12 core active components of XJQ and indicated its effect on cognitive dysfunction involved regulating synapses,inflammation,and phosphodiesterase 4(PDE4)-dependent cyclic adenosine monophosphate(cAMP)signaling.XJQ inhibited microglial and astrocyte activation,decreased proinflammatory cytokines,and mitigated neuronal damage.Notably,XJQ promoted synaptic repair and dendritic growth by downregulating PDE4 and upregulating cAMP,protein kinase A(PKA),cAMP-response element binding protein(CREB),brain-derived neurotrophic factor(BDNF),PSD95,and synapsin I levels.Molecular docking and Bio-layer interferometry assays confirmed direct binding of quercetin,kaempferol,isorhamnetin,and darutoside to PDE4.In conclusion,XJQ alleviates neuroinflammation and enhances synaptic plasticity to improve cognitive dysfunction in CHF rats via the PDE4/cAMP/PKA/CREB signaling pathway.These findings provide valuable insight into the heart-brain axis.展开更多
To ensure the safety, comfort, and effectiveness of lower limb rehabilitation exoskeleton robots in the rehabilitation training process, compliance is a prerequisite for human–machine interaction safety. First, under...To ensure the safety, comfort, and effectiveness of lower limb rehabilitation exoskeleton robots in the rehabilitation training process, compliance is a prerequisite for human–machine interaction safety. First, under the premise of considering the mechanical structure of the lower limb rehabilitation exoskeleton robot (LLRER), when conducting the dynamic transmission of the exoskeleton knee joint, the soft axis is added to ensure that the rotation motion and torque are flexibly transmitted to any position to achieve flexible force transmission. Second, to realize the active compliance control of LLRER, the sliding mode impedance closed-loop controller is developed based on the kinematics and dynamics model of LLRER, and the stability of the designed control system is verified by Lyapunov method. Then the experiment is designed to track the collected bicycle rehabilitation motion data stably, and the algorithm and dynamic model are verified to satisfy the experimental requirements. Finally, aiming at the transmission efficiency and response performance of the soft shaft in the torque transmission process of the knee joint, the soft shaft transmission performance test is carried out to test the soft shaft transmission performance and realize the compliance of the LLRER, so as to ensure that the rehabilitation training can be carried out in a safe and comfortable interactive environment. Through the design of rehabilitation exercise training, it is verified that the LLRER of flexible transmission under sliding mode impedance control has good adaptability in the actual environment, and can achieve accurate and flexible control. During the experiment, the effectiveness of monitoring rehabilitation training is brought through the respiratory belt.展开更多
This paper introduces a rigid-flexible coupling wearable exoskeleton robot for lower limb,which is designed in light of gait biomechanics and beneficial for low limb movement disorders by implementing gait training.Th...This paper introduces a rigid-flexible coupling wearable exoskeleton robot for lower limb,which is designed in light of gait biomechanics and beneficial for low limb movement disorders by implementing gait training.The rationality of the proposed mechanism is shown with the implementation of the dynamic simulation through MSC ADAMS.For the purposes of lightweight,the exoskeleton mechanism is optimized through finite element analysis.It can be concluded from performance evaluation experiment,the mechanism has certain advantages over existing exoskeleton robots,namely,comfortable,lightweight,low cost,which can be utilized for rehabilitation training in medical institutions or as a daily-walking ancillary equipment for patients.展开更多
Objective: To explore the effects of the somatosensory interaction technology combined with virtual reality technology on upper limbs function and activities of daily living (ADL) in cerebrovascular disease patients. ...Objective: To explore the effects of the somatosensory interaction technology combined with virtual reality technology on upper limbs function and activities of daily living (ADL) in cerebrovascular disease patients. Methods: Form January, 2019 to December, 2019, 80 cerebrovascular disease patients were recruited, and had been divided into control group (n = 40) and observation group (n = 40), randomly. The control groups received conventional rehabilitation treatment, for 40 minutes per day, while observation group received conventional rehabilitation treatment, for 20 minutes per day, and virtual reality technology treatment, 20 minutes per day, 5 days a week for 4 weeks. Wolf Motor Function Test (WMFT), Fugl-Meyer Assessment-Upper Extremities (FMA-UE) and modified Barthel index (MBI) were used to assess the motor function of the upper limbs and ADL before and after treatment. Results: Before treatment, the scores of WMFT, FMA-UE and MBI were no significant difference between two groups (P > 0.05). The scores improved in both groups after treatment (P < 0.01), and were higher in the observation group than in the control group (P < 0.05). Conclusion: The somatosensory interaction technology combined with virtual reality technology could facilitate to improve the upper limbs function and ADL in cerebrovascular disease patients.展开更多
短串联重复序列(short tandem repeat,STR)已广泛用于法医学亲子鉴定和个体识别中,但STR的突变可能会影响其结果的解释。在大多数类似研究中,由于忽略“隐性”突变现象,STR的突变率被低估。鉴于此,为获得更加准确的STR实际突变率,本研...短串联重复序列(short tandem repeat,STR)已广泛用于法医学亲子鉴定和个体识别中,但STR的突变可能会影响其结果的解释。在大多数类似研究中,由于忽略“隐性”突变现象,STR的突变率被低估。鉴于此,为获得更加准确的STR实际突变率,本研究使用Slooten与Ricciardi提出的有限突变模型和大规模数据,对28,313例(78,739个体)中国北京汉族已确认亲生关系的亲子鉴定案的20个常染色体STR基因座(D3S1358、D1S1656、D13S317、Penta E、D16S539、D18S51、D2S1338、CSF1PO、Penta D、TH01、vWA、D21S11、D6S1043、D7S820、D5S818、TPOX、D8S1179、D12S391、D19S433和FGA;由于有限突变模型中未包含D6S1043的矫正参数,因此本文实际计算其余19个STR基因座的突变率)进行了调查。结果发现,所有基因座均存在突变现象,总计发生1665个突变事件,包括1614个一步突变,34个两步突变,8个三步突变和9个非整步突变。基因座特异性的平均实际突变率在三联体中为0.00007700(TPOX)~0.00459050(FGA),在二联体中为0.00000000(TPOX)~0.00344850(FGA)。此外,本研究还分析了表面和实际突变率、三联体和二联体突变率、父源和母源的突变率之间的关系。研究表明,实际突变率多大于表面突变率,而且μ1^(*)/μ2^(*)(表面突变率)的比值通常也大于μ1/μ2(实际突变率)(μ1^(*),μ1;μ2^(*),μ2分别是一步和两步的突变率),即更多的“隐性”突变被释放出来。而且父源和母源的三联体和二联体的突变率也有存在差异。随后,将这些突变率数据与已发表的中国其他汉族人口的相关研究进行比较,展现出了STR突变率的时间与区域差异。由于样本量大,本研究中还报告了一些少见的突变事件,例如同卵双胞胎突变和“假四步突变”等。综上所述,本研究通过大量数据获得了接近真实的STR突变率的估计值,不仅可为中国法医DNA数据库和群体遗传学数据库提供重要的基础数据,也对开展法医学个体识别、亲权鉴定和遗传学研究具有重要的意义。展开更多
Since inferior biomimetic fabrication and anti-thrombosis process always lead to the implantation failure of artificial vascular grafts,the development of bioactive platform which can promote endothelialization is fun...Since inferior biomimetic fabrication and anti-thrombosis process always lead to the implantation failure of artificial vascular grafts,the development of bioactive platform which can promote endothelialization is fundamental in the research for blood vessel substitutes to overcome the low-efficiency or hyperplasia.To solve the limitation of insufficient autologous sources of small blood vessels,this study prepared a tri-layer small diameter vascular based on citrate,followed by covalently immobilizing of specific peptides to achieve in situ cells enrichment.The natural carbohydrate of carboxymethyl chitosan is creatively used as the inner layer of tubular graft,thus exerting the anti-thrombosis effect.Moreover,the gastrodin coating was employed to alleviate the inflammation response and increase the safety of this surgically implanted vascular scaffold.The synthesis process was characterized,its biocompatibility,hierarchically cell induction capacity,anticoagulant effect and the ability to promote neovascularization were evaluated.In such way,we hope to prepare a biodegradable small-diameter artificial vascular for the clinical application.展开更多
The application of non-noble metal catalysts in the catalytic direct gas-phase epoxidation of propylene with H2and O2to produce propylene oxide is valuable and challenging. The introduction of alkali metal promoters i...The application of non-noble metal catalysts in the catalytic direct gas-phase epoxidation of propylene with H2and O2to produce propylene oxide is valuable and challenging. The introduction of alkali metal promoters is one of the effective methods to improve the catalytic activity of catalysts. Herein, a series of alkali metal(Li, Na, K, Rb, and Cs)-promoted Ni/TS-1 catalysts were prepared to deeply understand the effect of alkali metals on the structure-activity relationship for gas-phase epoxidation of propylene. Among them, the Na-Ni/TS-1 catalyst exhibits the highest catalytic activity(propylene conversion of 7.35% and PO formation rate of 157.9 g h^(-1)kg_(cat)^(-1)) and the best stability(long-term stability exceeding 140 h at 200 ℃). X-ray absorption and photoelectron spectroscopy revealed that the electronic structure of Ni can be tuned by the addition of alkali metal promoters.NH3-TPD-MS, CO_(2)-TPD-MS, and C_(3)H_(6)-TPD-MS results indicate that the acidity of the catalyst can also be adjusted by the introduction of alkali metal, whereas the Na-Ni/TS-1 catalyst exhibits the strongest C_(3)H_(6) adsorption capacity. Thus, the suitable acid-base properties, unique electronic properties of Ni species, and the strongest propylene adsorption capacity resulted in improved propylene gas-phase epoxidation activity of Na-Ni/TS-1 catalyst. This study not only provides a new strategy for the practical application of nickel-based catalysts in the gas-phase epoxidation of propylene but also provides insights into the promoting effect of alkali metals.展开更多
Background:In forensic investigations,accurate estimation of the postmortem interval(PMI)is an important task,but also an ongoing challenge.Especially in cases where the cadaver has been specially treated,for example,...Background:In forensic investigations,accurate estimation of the postmortem interval(PMI)is an important task,but also an ongoing challenge.Especially in cases where the cadaver has been specially treated,for example,by boiling,the determination of PMI becomes extremely difficult.Previous studies have shown that the succession of the microbial community after decomposition of the cadaver can be used to infer PMI.However,the feasibility of determining the PMI of boiled cadavers has not yet been demonstrated.Aims and Objectives:The main objective of this study was to test whether we can infer PMI of boiled cadavers based on the succession of microbial communities.Materials and Methods:SD rats were killed by cervical dislocation.Subsequently,the rat cadavers were divided into the case(boiled cadavers)and control(unboiled cadavers)groups.Rectal samples were collected from the rats for 45 days and at nine time points.High-throughput sequencing of the 16S rRNA gene was performed to characterize the microbial community in the rectum.Results:The results showed that the composition and relative abundance of bacterial communities at the phylum level were significantly different between the case and control groups.The alpha diversity of the microbial community showed a decreasing trend with the decomposition process.Principal coordinate analysis showed that the case and control groups had obvious patterns along the succession of microbial communities.The rectal microbial communities showed a significant linear trend in the time course of decomposition.A random forest model was used to infer PMI.The goodness-of-fit(R2)of the model was 68.00%and 84.00%,and the mean absolute errors were 2.05 and 1.48 days within 45 days of decomposition for the case and control groups,respectively.Conclusions:Our results suggest that microbial community succession could be a potential method to infer PMI of boiled cadavers.展开更多
Cadmium(Cd)contamination in the environment is widespread,making it crucial to reduce Cd accumulation in cereal crops like wheat.However,strategies that not only mitigate Cd pollution but also address other environmen...Cadmium(Cd)contamination in the environment is widespread,making it crucial to reduce Cd accumulation in cereal crops like wheat.However,strategies that not only mitigate Cd pollution but also address other environmental challenges,such as invasive species management,remain unclear.This study introduces an innovative approach combining molybdenum nanoparticles(Mo NPs,1μM)and biochar biofilters derived from the invasive plant Mikania micrantha(IPMM),targeting the biochemical and molecular responses of wheat under Cd stress(100μM).Our findings showed that this novel combination significantly improved wheat physiological characteristics,growth,root architecture,elemental profile,osmoregulation,carotenoid,chlorophyll,gas exchange,and photosynthetic efficiency.Remarkably,simultaneous supply of IPMM biochar biofilters and Mo NPs substantially modulated the Cd translocation,reducing its accumulation in root(30.54%)and shoot(53.59%).Additionally,this strategy not only preserved mesophyll cell structures and the membrane integrity,but also strengthened and activated the oxidative defense systems through the regulation of genetic expressions.This synergistic approach advances the Cd alleviating techniques and offers a sustainable solution for utilizing invasive plants as a potential resource.By addressing both heavy metal pollution and ecological challenges,it provides a promising solution for safer crop production in Cd-contaminated environments.展开更多
基金supported by the National Key Research and Development Program of China(2022YFE0208300)the Natural Science Foundation of China(22078354)。
文摘The oxidative dehydrogenation of propane to propylene using CO_(2)(CO_(2)-ODH)offers a promising route for both propylene production and CO_(2)utilization.In this study,we investigate the effect of alkali metal doping on Pt-based catalysts in CO_(2)-ODH reactions.The optimized 0.1 KPt/S-1 catalyst achieved a high propane conversion of 48.3%,propylene selectivity of 85.5%,and CO_(2)conversion of 19.1%at a low temperature of 500℃with the Pt loading of 0.2 wt%and K loading of 0.1 wt%respectively.Characterization techniques,including high-resolution transmission electron microscope(HR-TEM),CO-diffuse reflectance infrared Fourier transform spectroscopy(CO-DRIFTS),X-ray absorption fine structure(XAFS),and X-ray Photoelectron Spectroscopy(XPS),revealed that the doping of K with Pt led to a strong interaction between potassium and platinum(Pt-KO_(x)cluster).This interaction resulted in a reduction of Pt particle size and a local enrichment of electron density around Pt atoms.These structural modifications improved the anchoring of Pt nanoparticles and enhanced Pt atom dispersion,thereby enhancing the activity of the catalyst and minimizing side reactions.Additionally,pyridine infrared(Py-IR)and temperature-programmed desorption(TPD)studies demonstrated that the prepared0.1 KPt/S-1 catalyst exhibited optimal acidity,which promoted C–H activation and facilitated the efficient adsorption and activation of CO_(2).These dual effects significantly lowered the activation energy for CO_(2)-ODH,enabling efficient dehydrogenation to propylene at a lower temperature of 500℃.This work highlights the critical role of alkali metal doping in modifying the electronic properties of Pt and optimizing catalyst acidity,which collectively contribute to the enhanced performance of the 0.1 KPt/S-1 catalyst.These findings offer valuable insights into the mechanistic pathway of CO_(2)-ODH and provide a foundation for the rational design of high-performance dehydrogenation catalysts.
基金financially supported by the National Natural Science Foundation of China(Nos.22178125 and 22478130).
文摘The interface instability between composite solid electrolytes(CSEs)and lithium anode significantly shortens the lifespan of all-solid-state lithium batteries(ASSLBs)with high areal capacity.In this work,a CSE featuring a trilayer architecture is developed by incorporating a thin polyethylene(PE)separator into a blending polymer matrix of poly(ethylene oxide)and poly(vinylidene fluoride)(PEO-PVDF)through a hot pressing technique.This structural design provides complementary functions:the flexible outer layers confine lithium deposition within a restricted area,while the robust interlayer prevents lithium dendrite penetration.Additionally,the incorporation of LiNO_(3) significantly enhances the stability of the CSE/Li interface by gradually forming a Li_(3)N-rich interfacial film,which promotes uniform lithium deposition.Consequently,the assembled Li||Li symmetrical cell demonstrates stable cycling for over 6000 h at a current density of 0.2 mA cm^(–2)with an areal capacity of 1.2 mAh cm^(–2).More attractively,ASSLBs constructed with the designed CSEs,high mass loading LFP/NCM811(LFP:LiFePO_(4);NCM811:LiNi_(0.8)Co_(0.1)Mn_(0.1)O_(2))cathodes(≥12 mg cm^(–2)),and lithium metal anodes deliver superior cycling performance without short-circuiting at current densities of 0.3/0.2 mA cm^(–2),respectively.This work offers critical insights for the design of high-performance ASSLBs with improved durability at high areal capacities.
基金supported by the National Natural Science Foundation of China(Nos.82430116 and 82574622)the Special Fund of Central Committee High Level Chinese Medicine Hospital(Nos.DZMG-LJRC-0014,DZMG-ZJXY-23013)+1 种基金Chinese Medicine Inheritance and Innovation“Thousand Million”Talents Project(Qihuang Project 2021)Qihuang Scholarsthe Medical and Health Industry Development Project of Tongzhou District(2023).
文摘Chronic heart failure(CHF)impairs cognitive function.Xijiaqi Formula(XJQ),a traditional Chinese medicine(TCM)used clinically to treat CHF,demonstrates potential for improving cognition in CHF patients.However,its precise mechanism in treating post-CHF cognitive dysfunction remains unclear.This study systematically investigates XJQ’s effects on post-CHF cognitive dysfunction and the underlying mechanisms.The components of XJQ were identified through liquid chromatography-mass spectrometry.CHF was induced in rats via ligation of the left anterior descending coronary artery,followed by six weeks of XJQ treatment.Cardiac function was evaluated through echocardiography and hemodynamic parameters,while cognitive function was assessed using Morris water maze(MWM)and open field tests(OFT).XJQ treatment enhanced both cardiac and cognitive functions in CHF rats.Network pharmacology identified 12 core active components of XJQ and indicated its effect on cognitive dysfunction involved regulating synapses,inflammation,and phosphodiesterase 4(PDE4)-dependent cyclic adenosine monophosphate(cAMP)signaling.XJQ inhibited microglial and astrocyte activation,decreased proinflammatory cytokines,and mitigated neuronal damage.Notably,XJQ promoted synaptic repair and dendritic growth by downregulating PDE4 and upregulating cAMP,protein kinase A(PKA),cAMP-response element binding protein(CREB),brain-derived neurotrophic factor(BDNF),PSD95,and synapsin I levels.Molecular docking and Bio-layer interferometry assays confirmed direct binding of quercetin,kaempferol,isorhamnetin,and darutoside to PDE4.In conclusion,XJQ alleviates neuroinflammation and enhances synaptic plasticity to improve cognitive dysfunction in CHF rats via the PDE4/cAMP/PKA/CREB signaling pathway.These findings provide valuable insight into the heart-brain axis.
基金supported in part by the National Natural Science Foundation of China under grants 61873304 and 62173048in part by the China Postdoctoral Science Foundation Funded Project under grant 2018M641784also in part by the Key Science and Technology Project of Jilin Province,China,grant nos.20200404208YY.
文摘To ensure the safety, comfort, and effectiveness of lower limb rehabilitation exoskeleton robots in the rehabilitation training process, compliance is a prerequisite for human–machine interaction safety. First, under the premise of considering the mechanical structure of the lower limb rehabilitation exoskeleton robot (LLRER), when conducting the dynamic transmission of the exoskeleton knee joint, the soft axis is added to ensure that the rotation motion and torque are flexibly transmitted to any position to achieve flexible force transmission. Second, to realize the active compliance control of LLRER, the sliding mode impedance closed-loop controller is developed based on the kinematics and dynamics model of LLRER, and the stability of the designed control system is verified by Lyapunov method. Then the experiment is designed to track the collected bicycle rehabilitation motion data stably, and the algorithm and dynamic model are verified to satisfy the experimental requirements. Finally, aiming at the transmission efficiency and response performance of the soft shaft in the torque transmission process of the knee joint, the soft shaft transmission performance test is carried out to test the soft shaft transmission performance and realize the compliance of the LLRER, so as to ensure that the rehabilitation training can be carried out in a safe and comfortable interactive environment. Through the design of rehabilitation exercise training, it is verified that the LLRER of flexible transmission under sliding mode impedance control has good adaptability in the actual environment, and can achieve accurate and flexible control. During the experiment, the effectiveness of monitoring rehabilitation training is brought through the respiratory belt.
基金The work is supported in part by the National Natural Science Foundation of China under Grants(61873304)in part by the China Postdoctoral Science Foundation Funded Project under Grant(2018M641784)also in part by the Funding of Jilin Province Science and Technology(JJKH20210745KJ).
文摘This paper introduces a rigid-flexible coupling wearable exoskeleton robot for lower limb,which is designed in light of gait biomechanics and beneficial for low limb movement disorders by implementing gait training.The rationality of the proposed mechanism is shown with the implementation of the dynamic simulation through MSC ADAMS.For the purposes of lightweight,the exoskeleton mechanism is optimized through finite element analysis.It can be concluded from performance evaluation experiment,the mechanism has certain advantages over existing exoskeleton robots,namely,comfortable,lightweight,low cost,which can be utilized for rehabilitation training in medical institutions or as a daily-walking ancillary equipment for patients.
文摘Objective: To explore the effects of the somatosensory interaction technology combined with virtual reality technology on upper limbs function and activities of daily living (ADL) in cerebrovascular disease patients. Methods: Form January, 2019 to December, 2019, 80 cerebrovascular disease patients were recruited, and had been divided into control group (n = 40) and observation group (n = 40), randomly. The control groups received conventional rehabilitation treatment, for 40 minutes per day, while observation group received conventional rehabilitation treatment, for 20 minutes per day, and virtual reality technology treatment, 20 minutes per day, 5 days a week for 4 weeks. Wolf Motor Function Test (WMFT), Fugl-Meyer Assessment-Upper Extremities (FMA-UE) and modified Barthel index (MBI) were used to assess the motor function of the upper limbs and ADL before and after treatment. Results: Before treatment, the scores of WMFT, FMA-UE and MBI were no significant difference between two groups (P > 0.05). The scores improved in both groups after treatment (P < 0.01), and were higher in the observation group than in the control group (P < 0.05). Conclusion: The somatosensory interaction technology combined with virtual reality technology could facilitate to improve the upper limbs function and ADL in cerebrovascular disease patients.
文摘短串联重复序列(short tandem repeat,STR)已广泛用于法医学亲子鉴定和个体识别中,但STR的突变可能会影响其结果的解释。在大多数类似研究中,由于忽略“隐性”突变现象,STR的突变率被低估。鉴于此,为获得更加准确的STR实际突变率,本研究使用Slooten与Ricciardi提出的有限突变模型和大规模数据,对28,313例(78,739个体)中国北京汉族已确认亲生关系的亲子鉴定案的20个常染色体STR基因座(D3S1358、D1S1656、D13S317、Penta E、D16S539、D18S51、D2S1338、CSF1PO、Penta D、TH01、vWA、D21S11、D6S1043、D7S820、D5S818、TPOX、D8S1179、D12S391、D19S433和FGA;由于有限突变模型中未包含D6S1043的矫正参数,因此本文实际计算其余19个STR基因座的突变率)进行了调查。结果发现,所有基因座均存在突变现象,总计发生1665个突变事件,包括1614个一步突变,34个两步突变,8个三步突变和9个非整步突变。基因座特异性的平均实际突变率在三联体中为0.00007700(TPOX)~0.00459050(FGA),在二联体中为0.00000000(TPOX)~0.00344850(FGA)。此外,本研究还分析了表面和实际突变率、三联体和二联体突变率、父源和母源的突变率之间的关系。研究表明,实际突变率多大于表面突变率,而且μ1^(*)/μ2^(*)(表面突变率)的比值通常也大于μ1/μ2(实际突变率)(μ1^(*),μ1;μ2^(*),μ2分别是一步和两步的突变率),即更多的“隐性”突变被释放出来。而且父源和母源的三联体和二联体的突变率也有存在差异。随后,将这些突变率数据与已发表的中国其他汉族人口的相关研究进行比较,展现出了STR突变率的时间与区域差异。由于样本量大,本研究中还报告了一些少见的突变事件,例如同卵双胞胎突变和“假四步突变”等。综上所述,本研究通过大量数据获得了接近真实的STR突变率的估计值,不仅可为中国法医DNA数据库和群体遗传学数据库提供重要的基础数据,也对开展法医学个体识别、亲权鉴定和遗传学研究具有重要的意义。
基金supported by the National Natural Science Foundation of China(grant NO.82260088/81760087)Major Program of Kunming Science and Technology Innovation Center(grant NO.2019-1-N-25318000003568)+3 种基金the Applied Basic Research Foundation of Yunnan Province(grant NO.202201AY070001-029/202201AY070001-030/202001AY070001-014/202401AY070001-030)First-Class Discipline Team of Kunming Medical University(2024XKTDYS01)Yunnan Pro-vincial Innovative Research Team in Basic and Clinical Study of Coronary Heart Disease of Yunnan Revitalization Talent Support Program(202305AS350030)the Foundation of Expert Workstation of Bai Xiaochun(YSZJGZZ-2020040).
文摘Since inferior biomimetic fabrication and anti-thrombosis process always lead to the implantation failure of artificial vascular grafts,the development of bioactive platform which can promote endothelialization is fundamental in the research for blood vessel substitutes to overcome the low-efficiency or hyperplasia.To solve the limitation of insufficient autologous sources of small blood vessels,this study prepared a tri-layer small diameter vascular based on citrate,followed by covalently immobilizing of specific peptides to achieve in situ cells enrichment.The natural carbohydrate of carboxymethyl chitosan is creatively used as the inner layer of tubular graft,thus exerting the anti-thrombosis effect.Moreover,the gastrodin coating was employed to alleviate the inflammation response and increase the safety of this surgically implanted vascular scaffold.The synthesis process was characterized,its biocompatibility,hierarchically cell induction capacity,anticoagulant effect and the ability to promote neovascularization were evaluated.In such way,we hope to prepare a biodegradable small-diameter artificial vascular for the clinical application.
基金supported by the National Key Research and Development Program of China (2022YFE0208300)the National Natural Science Foundation of China (22078354, 22108290)。
文摘The application of non-noble metal catalysts in the catalytic direct gas-phase epoxidation of propylene with H2and O2to produce propylene oxide is valuable and challenging. The introduction of alkali metal promoters is one of the effective methods to improve the catalytic activity of catalysts. Herein, a series of alkali metal(Li, Na, K, Rb, and Cs)-promoted Ni/TS-1 catalysts were prepared to deeply understand the effect of alkali metals on the structure-activity relationship for gas-phase epoxidation of propylene. Among them, the Na-Ni/TS-1 catalyst exhibits the highest catalytic activity(propylene conversion of 7.35% and PO formation rate of 157.9 g h^(-1)kg_(cat)^(-1)) and the best stability(long-term stability exceeding 140 h at 200 ℃). X-ray absorption and photoelectron spectroscopy revealed that the electronic structure of Ni can be tuned by the addition of alkali metal promoters.NH3-TPD-MS, CO_(2)-TPD-MS, and C_(3)H_(6)-TPD-MS results indicate that the acidity of the catalyst can also be adjusted by the introduction of alkali metal, whereas the Na-Ni/TS-1 catalyst exhibits the strongest C_(3)H_(6) adsorption capacity. Thus, the suitable acid-base properties, unique electronic properties of Ni species, and the strongest propylene adsorption capacity resulted in improved propylene gas-phase epoxidation activity of Na-Ni/TS-1 catalyst. This study not only provides a new strategy for the practical application of nickel-based catalysts in the gas-phase epoxidation of propylene but also provides insights into the promoting effect of alkali metals.
基金supported by the National Natural Science Foundation of China(82030058,82101977,82130056).
文摘Background:In forensic investigations,accurate estimation of the postmortem interval(PMI)is an important task,but also an ongoing challenge.Especially in cases where the cadaver has been specially treated,for example,by boiling,the determination of PMI becomes extremely difficult.Previous studies have shown that the succession of the microbial community after decomposition of the cadaver can be used to infer PMI.However,the feasibility of determining the PMI of boiled cadavers has not yet been demonstrated.Aims and Objectives:The main objective of this study was to test whether we can infer PMI of boiled cadavers based on the succession of microbial communities.Materials and Methods:SD rats were killed by cervical dislocation.Subsequently,the rat cadavers were divided into the case(boiled cadavers)and control(unboiled cadavers)groups.Rectal samples were collected from the rats for 45 days and at nine time points.High-throughput sequencing of the 16S rRNA gene was performed to characterize the microbial community in the rectum.Results:The results showed that the composition and relative abundance of bacterial communities at the phylum level were significantly different between the case and control groups.The alpha diversity of the microbial community showed a decreasing trend with the decomposition process.Principal coordinate analysis showed that the case and control groups had obvious patterns along the succession of microbial communities.The rectal microbial communities showed a significant linear trend in the time course of decomposition.A random forest model was used to infer PMI.The goodness-of-fit(R2)of the model was 68.00%and 84.00%,and the mean absolute errors were 2.05 and 1.48 days within 45 days of decomposition for the case and control groups,respectively.Conclusions:Our results suggest that microbial community succession could be a potential method to infer PMI of boiled cadavers.
文摘Cadmium(Cd)contamination in the environment is widespread,making it crucial to reduce Cd accumulation in cereal crops like wheat.However,strategies that not only mitigate Cd pollution but also address other environmental challenges,such as invasive species management,remain unclear.This study introduces an innovative approach combining molybdenum nanoparticles(Mo NPs,1μM)and biochar biofilters derived from the invasive plant Mikania micrantha(IPMM),targeting the biochemical and molecular responses of wheat under Cd stress(100μM).Our findings showed that this novel combination significantly improved wheat physiological characteristics,growth,root architecture,elemental profile,osmoregulation,carotenoid,chlorophyll,gas exchange,and photosynthetic efficiency.Remarkably,simultaneous supply of IPMM biochar biofilters and Mo NPs substantially modulated the Cd translocation,reducing its accumulation in root(30.54%)and shoot(53.59%).Additionally,this strategy not only preserved mesophyll cell structures and the membrane integrity,but also strengthened and activated the oxidative defense systems through the regulation of genetic expressions.This synergistic approach advances the Cd alleviating techniques and offers a sustainable solution for utilizing invasive plants as a potential resource.By addressing both heavy metal pollution and ecological challenges,it provides a promising solution for safer crop production in Cd-contaminated environments.
