为了筛选影响发情卵巢发育的关键蛋白,解析彭波半细毛羊繁殖的调控机制,该研究采用TMT(tandem mass tags)蛋白质组学技术对不同发情状态彭波半细毛羊的卵巢进行蛋白质组测序,通过比较分析彭波半细毛羊发情和乏情卵巢蛋白组数据共筛选出...为了筛选影响发情卵巢发育的关键蛋白,解析彭波半细毛羊繁殖的调控机制,该研究采用TMT(tandem mass tags)蛋白质组学技术对不同发情状态彭波半细毛羊的卵巢进行蛋白质组测序,通过比较分析彭波半细毛羊发情和乏情卵巢蛋白组数据共筛选出307个差异表达蛋白,其中下调的差异蛋白为249个(P<0.05),上调的差异蛋白为58个(P<0.05)。这些差异蛋白涉及多种GO分类及KEGG通路。其中GO分类注释显示,差异蛋白与谷胱甘肽转移酶活性,酰基辅酶A脱氢酶活性和磷脂酰肌醇结合等分子功能通路存在密切关联。KEGG通路分析显示,剪切体通路富集水平最高,其中包括SF3A2、PRPF19、BUD31等剪切体通路上的蛋白在细胞生长和分化、凋亡等生物过程中发挥重要作用。同时也发现补体和凝血级联、谷胱甘肽代谢以及细胞色素P450相关通路也表现出显著富集。这些结果为进一步阐明彭波半细毛羊发情和乏情卵巢组织的生理功能变化提供了基础,同时也为全面了解彭波半细毛羊发情规律提供新的参考。展开更多
Dissimilar AZ31B magnesium alloy and DC56D steel were welded via AA1060 aluminum alloy by magnetic pulse welding.The effects of primary and secondary welding processes on the welded interface were comparatively invest...Dissimilar AZ31B magnesium alloy and DC56D steel were welded via AA1060 aluminum alloy by magnetic pulse welding.The effects of primary and secondary welding processes on the welded interface were comparatively investigated.Macroscopic morphology,microstructure,and interfacial structure of the joints were analyzed using scanning electron microscope,energy dispersive spectrometer,and X-ray diffractometer(XRD).The results show that magnetic pulse welding of dissimilar Mg/Fe metals is achieved using an Al interlayer,which acts as a bridge for deformation and diffusion.Specifically,the AZ31B/AA1060 interface exhibits a typical wavy morphology,and a transition zone exists at the joint interface,which may result in an extremely complex microstructure.The microstructure of this transition zone differs from that of AZ31B magnesium and 1060 Al alloys,and it is identified as brittle intermetallic compounds(IMCs)Al_(3)Mg_(2) and Al_(12)Mg_(17).The transition zone is mainly distributed on the Al side,with the maximum thickness of Al-side transition layer reaching approximately 13.53μm.Incomplete melting layers with varying thicknesses are observed at the primary weld interface,while micron-sized hole defects appear in the transition zone of the secondary weld interface.The AA1060/DC56D interface is mainly straight,with only a small number of discontinuous transition zones distributed intermittently along the interface.These transition zones are characterized by the presence of the brittle IMC FeAl_(3),with a maximum thickness of about 4μm.展开更多
文摘为了筛选影响发情卵巢发育的关键蛋白,解析彭波半细毛羊繁殖的调控机制,该研究采用TMT(tandem mass tags)蛋白质组学技术对不同发情状态彭波半细毛羊的卵巢进行蛋白质组测序,通过比较分析彭波半细毛羊发情和乏情卵巢蛋白组数据共筛选出307个差异表达蛋白,其中下调的差异蛋白为249个(P<0.05),上调的差异蛋白为58个(P<0.05)。这些差异蛋白涉及多种GO分类及KEGG通路。其中GO分类注释显示,差异蛋白与谷胱甘肽转移酶活性,酰基辅酶A脱氢酶活性和磷脂酰肌醇结合等分子功能通路存在密切关联。KEGG通路分析显示,剪切体通路富集水平最高,其中包括SF3A2、PRPF19、BUD31等剪切体通路上的蛋白在细胞生长和分化、凋亡等生物过程中发挥重要作用。同时也发现补体和凝血级联、谷胱甘肽代谢以及细胞色素P450相关通路也表现出显著富集。这些结果为进一步阐明彭波半细毛羊发情和乏情卵巢组织的生理功能变化提供了基础,同时也为全面了解彭波半细毛羊发情规律提供新的参考。
文摘Dissimilar AZ31B magnesium alloy and DC56D steel were welded via AA1060 aluminum alloy by magnetic pulse welding.The effects of primary and secondary welding processes on the welded interface were comparatively investigated.Macroscopic morphology,microstructure,and interfacial structure of the joints were analyzed using scanning electron microscope,energy dispersive spectrometer,and X-ray diffractometer(XRD).The results show that magnetic pulse welding of dissimilar Mg/Fe metals is achieved using an Al interlayer,which acts as a bridge for deformation and diffusion.Specifically,the AZ31B/AA1060 interface exhibits a typical wavy morphology,and a transition zone exists at the joint interface,which may result in an extremely complex microstructure.The microstructure of this transition zone differs from that of AZ31B magnesium and 1060 Al alloys,and it is identified as brittle intermetallic compounds(IMCs)Al_(3)Mg_(2) and Al_(12)Mg_(17).The transition zone is mainly distributed on the Al side,with the maximum thickness of Al-side transition layer reaching approximately 13.53μm.Incomplete melting layers with varying thicknesses are observed at the primary weld interface,while micron-sized hole defects appear in the transition zone of the secondary weld interface.The AA1060/DC56D interface is mainly straight,with only a small number of discontinuous transition zones distributed intermittently along the interface.These transition zones are characterized by the presence of the brittle IMC FeAl_(3),with a maximum thickness of about 4μm.
