In the present study, a new ceramide, namely 2S, 3R-4E, 8E-2-(heptadecanoylamino)-heptadeca-4, 8-diene-1, 3-diol(1), along with four known steroids, including 24-methylcholesta-5, 24(28)-diene-3β-ol(2), 24-methylchol...In the present study, a new ceramide, namely 2S, 3R-4E, 8E-2-(heptadecanoylamino)-heptadeca-4, 8-diene-1, 3-diol(1), along with four known steroids, including 24-methylcholesta-5, 24(28)-diene-3β-ol(2), 24-methylcholesta-5, 24(28)-diene-3β-acetate(3), 4-methyl-24-methylcholesta- 22-ene-3-ol(4), and cholesterol, was isolated and characterized from CH2Cl2/Me OH extract of Cespitularia stolonifera. A new acetate derivative of compound 1, termed 2S, 3R-4E, 8E-2-(heptadecanoylamino)-heptadeca-4, 8-diene-1, 3-diacetate(1a), was also prepared in the present study. All the structures were established on the basis of modern spectroscopic techniques, including FT-IR, 1D, 2D-NMR, HRESI-MS, and GC-MS, in addition of chemical methods.(-)-Alloaromadendren, ledane,(1)-alloaromadendren oxide, isoaromadendrene epoxide and(-)-caryophellen oxide were identified from the n-hexane fraction using GC-MS. The extract and the two ceramides(1) and(1a) exhibited significant cytotoxic activity against lung cancer A549 cells, while the extract and the two steroids(2) and(3) exhibited significant cytotoxic activity against breast cancer MCF-7 cells. The CH_2Cl_2/MeOH extract exhibited significant antiulcer activity in both ethanol and acetic acid induced ulcer models in rats, as evidenced by histopathological, histochemical, and biochemical examinations.展开更多
Evaluation of the anti-herpetic activity of 25 Egyptian plants extracts was investigated in vitro in this study.The antiviral activity against Herpes Simplex Virus type 1(HSV-1)was done on Vero cell lines by cell viab...Evaluation of the anti-herpetic activity of 25 Egyptian plants extracts was investigated in vitro in this study.The antiviral activity against Herpes Simplex Virus type 1(HSV-1)was done on Vero cell lines by cell viability.Only two plants extracts;namely Euphorbia coopire(Euphorbiaceae)and Morus alba(Moraceae)showed potent anti-herpetic activity and six other extracts showed moderate inhibition.In contrast,a bioassay monitored phytochemical exploration of these two plants led to the isolation of pure flavonoid compounds.The antiviral activity of the isolated compounds was also examined,among which seven pure compounds namely;7-galloyl catechin,gallic acid,kaempferol 3-O-β-(6″-O-galloyl)-glucopyranoside,quercetin 3-O-β-(6″-O-galloyl)-glucopyranoside,curcumin,quercetin and kaempferol exhibited significant inhibition.展开更多
Objective:To isolate and identify the polyphenolic constituents of Dypsis lutescens,and evaluate the hepatoprotective activity of the ethanolic extract of Dypsis lutescens leaves.Methods:Hepatoprotective,antioxidant a...Objective:To isolate and identify the polyphenolic constituents of Dypsis lutescens,and evaluate the hepatoprotective activity of the ethanolic extract of Dypsis lutescens leaves.Methods:Hepatoprotective,antioxidant and anti-inflammatory effects of two doses of Dypsis lutescens ethanolic leaf extract were investigated in five groups of six rats each administered with the ethanolic extract of Dypsis lutescens leaves.Liver function parameters were assessed,histopathological study was carried out,the anti-inflammatory mediators and the antioxidant potential in the liver tissues were evaluated.In addition,the total ethanolic extract of Dypsis lutescens leaves was subjected to different chromatographic separation techniques to yield ten phenolic compounds.The isolated compounds structures were spectroscopically elucidated.Results:Hepatoprotective activity of Dypsis lutescens ethanolic extract was estimated for the first time and showed significant activity against histopathological changes induced by D-galactosamine in liver.The extract improved the liver functions.Compared to the D-galactosamine group,the architecture of the liver in the treated groups was improved in the histopathological examination.These results proved the hepatoprotective activity of Dypsis lutescens and its ability in attenuating liver oxidative damage and inflammation.Phytochemical investigations of the total extract afforded ten compounds from the genus Dypsis.Conclusions:The alcoholic extract of Dypsis lutescens exerted potential hepatoprotective action,maintaining liver health and functions.展开更多
基金supported by the Science and Technology Development Fund(STDF)(No.6554)
文摘In the present study, a new ceramide, namely 2S, 3R-4E, 8E-2-(heptadecanoylamino)-heptadeca-4, 8-diene-1, 3-diol(1), along with four known steroids, including 24-methylcholesta-5, 24(28)-diene-3β-ol(2), 24-methylcholesta-5, 24(28)-diene-3β-acetate(3), 4-methyl-24-methylcholesta- 22-ene-3-ol(4), and cholesterol, was isolated and characterized from CH2Cl2/Me OH extract of Cespitularia stolonifera. A new acetate derivative of compound 1, termed 2S, 3R-4E, 8E-2-(heptadecanoylamino)-heptadeca-4, 8-diene-1, 3-diacetate(1a), was also prepared in the present study. All the structures were established on the basis of modern spectroscopic techniques, including FT-IR, 1D, 2D-NMR, HRESI-MS, and GC-MS, in addition of chemical methods.(-)-Alloaromadendren, ledane,(1)-alloaromadendren oxide, isoaromadendrene epoxide and(-)-caryophellen oxide were identified from the n-hexane fraction using GC-MS. The extract and the two ceramides(1) and(1a) exhibited significant cytotoxic activity against lung cancer A549 cells, while the extract and the two steroids(2) and(3) exhibited significant cytotoxic activity against breast cancer MCF-7 cells. The CH_2Cl_2/MeOH extract exhibited significant antiulcer activity in both ethanol and acetic acid induced ulcer models in rats, as evidenced by histopathological, histochemical, and biochemical examinations.
基金The authors thank the Academy of Scientific Research&Technology(ASRT,Egypt)and Centre de Recherchéet d’enseignement Yves Coppens,Campus de Tohannic,BP 573,56017 Vannes(France)for supporting this study within the IMHOTEP program(Project No.EGY/FR 8-013).
文摘Evaluation of the anti-herpetic activity of 25 Egyptian plants extracts was investigated in vitro in this study.The antiviral activity against Herpes Simplex Virus type 1(HSV-1)was done on Vero cell lines by cell viability.Only two plants extracts;namely Euphorbia coopire(Euphorbiaceae)and Morus alba(Moraceae)showed potent anti-herpetic activity and six other extracts showed moderate inhibition.In contrast,a bioassay monitored phytochemical exploration of these two plants led to the isolation of pure flavonoid compounds.The antiviral activity of the isolated compounds was also examined,among which seven pure compounds namely;7-galloyl catechin,gallic acid,kaempferol 3-O-β-(6″-O-galloyl)-glucopyranoside,quercetin 3-O-β-(6″-O-galloyl)-glucopyranoside,curcumin,quercetin and kaempferol exhibited significant inhibition.
基金fund for this work has been received from the National Research Centre,Egypt(project No.11010325).
文摘Objective:To isolate and identify the polyphenolic constituents of Dypsis lutescens,and evaluate the hepatoprotective activity of the ethanolic extract of Dypsis lutescens leaves.Methods:Hepatoprotective,antioxidant and anti-inflammatory effects of two doses of Dypsis lutescens ethanolic leaf extract were investigated in five groups of six rats each administered with the ethanolic extract of Dypsis lutescens leaves.Liver function parameters were assessed,histopathological study was carried out,the anti-inflammatory mediators and the antioxidant potential in the liver tissues were evaluated.In addition,the total ethanolic extract of Dypsis lutescens leaves was subjected to different chromatographic separation techniques to yield ten phenolic compounds.The isolated compounds structures were spectroscopically elucidated.Results:Hepatoprotective activity of Dypsis lutescens ethanolic extract was estimated for the first time and showed significant activity against histopathological changes induced by D-galactosamine in liver.The extract improved the liver functions.Compared to the D-galactosamine group,the architecture of the liver in the treated groups was improved in the histopathological examination.These results proved the hepatoprotective activity of Dypsis lutescens and its ability in attenuating liver oxidative damage and inflammation.Phytochemical investigations of the total extract afforded ten compounds from the genus Dypsis.Conclusions:The alcoholic extract of Dypsis lutescens exerted potential hepatoprotective action,maintaining liver health and functions.
