The endogenous hormones (EHs) content of different explants (anther, young panicle, young embryo and mature embryo) and calli with different culture capability were analyzed by means of high performance liquid chromat...The endogenous hormones (EHs) content of different explants (anther, young panicle, young embryo and mature embryo) and calli with different culture capability were analyzed by means of high performance liquid chromatography (HPLC). The results showed that the contents and ratio of endogenous hormones were one of the key factors affecting callus induction frequencies (CIF) and green plantlet differentiation frequencies (GPDF). The influence of endogenous hormones of different explants on CIF represented as: Zoatin ribosile (ZR) showed negative effect, indole-3-acetic acid (IAA) did positive effect, and gibberellic acid (GA) did negative effect except for mature embryos. The influence of endogenous hormones on green plantlet differentiation frequency (GPDF) showed: IAA and GA were negative effect; abscisic acid (ABA) and zeatin+ zeatin riboside (Z+ZR) were positive effect. The mixture ratio of endogenous hormones played a role on CIF and GPDF. IAA/Z+ZR had a positive effect on CIF, and there was a notable positive correlation between Z+ZR/ IAA and GPDF, so was between ABA/IAA and GPDF.展开更多
A spontaneously occurring rice (Oryza sativa L.) mutant, characterized by homeotic conversionin glumes and stamens, was found in the progeny of a cross. The mutant showed long glumes andglumaceous lodicules and morpho...A spontaneously occurring rice (Oryza sativa L.) mutant, characterized by homeotic conversionin glumes and stamens, was found in the progeny of a cross. The mutant showed long glumes andglumaceous lodicules and morphological transformation of stamens into pistils. Mutant floretsconsisted of 1 to 3 completely developed pistils, some pistilloid stamens with filaments, buttipped by bulged tissue and 0 to 3 stigmas. It seems that the mutant phenotype of the homeoticconversions in glumes and stamens is similar to that of the B loss-of-function mutants inArabidopsis and Antirrhinum. The mutant is controlled by a single recessive gene as asegregation ratio of 3:1 (wild type to mutant plants) was observed in the F2 generation.展开更多
文摘The endogenous hormones (EHs) content of different explants (anther, young panicle, young embryo and mature embryo) and calli with different culture capability were analyzed by means of high performance liquid chromatography (HPLC). The results showed that the contents and ratio of endogenous hormones were one of the key factors affecting callus induction frequencies (CIF) and green plantlet differentiation frequencies (GPDF). The influence of endogenous hormones of different explants on CIF represented as: Zoatin ribosile (ZR) showed negative effect, indole-3-acetic acid (IAA) did positive effect, and gibberellic acid (GA) did negative effect except for mature embryos. The influence of endogenous hormones on green plantlet differentiation frequency (GPDF) showed: IAA and GA were negative effect; abscisic acid (ABA) and zeatin+ zeatin riboside (Z+ZR) were positive effect. The mixture ratio of endogenous hormones played a role on CIF and GPDF. IAA/Z+ZR had a positive effect on CIF, and there was a notable positive correlation between Z+ZR/ IAA and GPDF, so was between ABA/IAA and GPDF.
文摘A spontaneously occurring rice (Oryza sativa L.) mutant, characterized by homeotic conversionin glumes and stamens, was found in the progeny of a cross. The mutant showed long glumes andglumaceous lodicules and morphological transformation of stamens into pistils. Mutant floretsconsisted of 1 to 3 completely developed pistils, some pistilloid stamens with filaments, buttipped by bulged tissue and 0 to 3 stigmas. It seems that the mutant phenotype of the homeoticconversions in glumes and stamens is similar to that of the B loss-of-function mutants inArabidopsis and Antirrhinum. The mutant is controlled by a single recessive gene as asegregation ratio of 3:1 (wild type to mutant plants) was observed in the F2 generation.
