Objective:To detect the inhibiting co-stimulating molecule CTLA4 and cytokines secreted by Treg cells, and explore the immunology mechanism of T regulatory cells acting on effector T cells in co-cultured system(CCS) a...Objective:To detect the inhibiting co-stimulating molecule CTLA4 and cytokines secreted by Treg cells, and explore the immunology mechanism of T regulatory cells acting on effector T cells in co-cultured system(CCS) and separating-cultured system(SCS). Methods: Detecting the percentage of CTLA4 and CD28 expressed on the Treg cells and effector T cells, and then adding Treg cells to mixed lymphocyte reaction(MLR) system in CCS and TransWell Millicell-PCF SCS, at the same time, adding or not adding anti-IL-10 or anti-TGF-β1 to the reacting systems, examining the inhibitory capacity of Treg cells exerting on the MLR. Results: Compared with effector T cells, Treg cells expressed higher level CTLA4 and secreted much more IL-10 and TGF-β1(P<0.01). The inhibitory capacity of Treg cells co-cultured with effector T cells is much stronger than that in separating cultured group(P<0.01). Moreover, the inhibiting rate of Treg cells exerting on effector T cells through secreting IL-10 was more powerful than that through secreting TGF-β1(P<0.01). Conclusion: Both cell-to-cell contact and cytokines secretion mechanisms are involved in CD4 +CD25 + Treg cells operating function. However, the former is more important. Intrestingly, we for the first time point found that IL-10 plays more powerful roles than TGF-β1 in the cytokines secretion mechanism.展开更多
In the cross between B. napus with OguCMSOrychophragmus violaceus, pre-fertilizatiocompatibility was observed. An intergeneric hybrid was successfully obtained by usinovary culture. The hybrid status was confirmed by ...In the cross between B. napus with OguCMSOrychophragmus violaceus, pre-fertilizatiocompatibility was observed. An intergeneric hybrid was successfully obtained by usinovary culture. The hybrid status was confirmed by cytology, morphology, fertilityisozyme and miosis analysis. The seed setting rate was 0.0072%. An amphidiploid of thhybrid was also produced by the use of chromosome-doubling method with 0.1% colchicinetreatment. Obtaining this hybrid and its amphidiploid has important theoretical anutilization value.展开更多
Texture extract from digital aerial image is widely used in three-dimensional city modeling to generate “photo-realistic” views. In this paper, a method based on reforming “Steep edge” curve, which clearly explain...Texture extract from digital aerial image is widely used in three-dimensional city modeling to generate “photo-realistic” views. In this paper, a method based on reforming “Steep edge” curve, which clearly explains how the diffraction of the sunlight makes digital aerial image blurring, is proposed to deblur the texture extraction from digital aerial image, and the experiment shows a good result in visualization and automation.展开更多
Abstract Although among the modem Chinese intellectuals endeavoring for the enlightenment of the people, Lu Xun is the most rebellious and resolute, his rebelliousness against tradition does not mean that he has nothi...Abstract Although among the modem Chinese intellectuals endeavoring for the enlightenment of the people, Lu Xun is the most rebellious and resolute, his rebelliousness against tradition does not mean that he has nothing to do with tradition itself. On the contrary, in order to fight against a tradition, as a precondition he must have a deep understanding and cognition toward that tradition. The emergence ofLu Xun＇s philosophical proposition, ＂everything is an intermediate object＂ （yiqie doushi zhon^ffianw~, occurs exactly in this way. With the evocation of this philosophical thought, the ＂intermediate object＂ （zhon^ianwto, we see the inseparable indigenous tie predestined between Lu Xun and Chinese traditional culture, even while he fiercely fights it. Lu Xun＇s innovative idea was produced in the process of deducing and developing the excellent and discarding the worthless in Chinese traditional culture, while absorbing and learning from the advanced thought of the West. Furthermore, his philosophy of the ＂intermediate object＂ forms the basis of his study and practice in translation. His purpose in translation is to bravely step out of the circle of inherent traditional culture, to come to the advanced ＂middle zone＂ where Chinese and Western cultures collide, and to probe into the new cultural factors from the West. In doing so he seeks to reform and improve Chinese traditional culture, and thus meet ＂the third era which China has never experienced before.＂ However, Lu Xun＇s idea of ＂intermediate objects＂ is neither the traditional idea of the ＂golden mean＂ （zhongyong zhidao） nor that of ＂hypocrisy＂ （xiangyuanyuan 乡愿）. Unfortunately they are often mixed together into chaos by many people. So it is necessary to have further discussion about these terms and distinguish them separately.展开更多
文摘Objective:To detect the inhibiting co-stimulating molecule CTLA4 and cytokines secreted by Treg cells, and explore the immunology mechanism of T regulatory cells acting on effector T cells in co-cultured system(CCS) and separating-cultured system(SCS). Methods: Detecting the percentage of CTLA4 and CD28 expressed on the Treg cells and effector T cells, and then adding Treg cells to mixed lymphocyte reaction(MLR) system in CCS and TransWell Millicell-PCF SCS, at the same time, adding or not adding anti-IL-10 or anti-TGF-β1 to the reacting systems, examining the inhibitory capacity of Treg cells exerting on the MLR. Results: Compared with effector T cells, Treg cells expressed higher level CTLA4 and secreted much more IL-10 and TGF-β1(P<0.01). The inhibitory capacity of Treg cells co-cultured with effector T cells is much stronger than that in separating cultured group(P<0.01). Moreover, the inhibiting rate of Treg cells exerting on effector T cells through secreting IL-10 was more powerful than that through secreting TGF-β1(P<0.01). Conclusion: Both cell-to-cell contact and cytokines secretion mechanisms are involved in CD4 +CD25 + Treg cells operating function. However, the former is more important. Intrestingly, we for the first time point found that IL-10 plays more powerful roles than TGF-β1 in the cytokines secretion mechanism.
基金supported by the National Natura1 Science Foundation of China(39470457) the Ninth Five-Year Major Program of China.
文摘In the cross between B. napus with OguCMSOrychophragmus violaceus, pre-fertilizatiocompatibility was observed. An intergeneric hybrid was successfully obtained by usinovary culture. The hybrid status was confirmed by cytology, morphology, fertilityisozyme and miosis analysis. The seed setting rate was 0.0072%. An amphidiploid of thhybrid was also produced by the use of chromosome-doubling method with 0.1% colchicinetreatment. Obtaining this hybrid and its amphidiploid has important theoretical anutilization value.
文摘Texture extract from digital aerial image is widely used in three-dimensional city modeling to generate “photo-realistic” views. In this paper, a method based on reforming “Steep edge” curve, which clearly explains how the diffraction of the sunlight makes digital aerial image blurring, is proposed to deblur the texture extraction from digital aerial image, and the experiment shows a good result in visualization and automation.
文摘Abstract Although among the modem Chinese intellectuals endeavoring for the enlightenment of the people, Lu Xun is the most rebellious and resolute, his rebelliousness against tradition does not mean that he has nothing to do with tradition itself. On the contrary, in order to fight against a tradition, as a precondition he must have a deep understanding and cognition toward that tradition. The emergence ofLu Xun＇s philosophical proposition, ＂everything is an intermediate object＂ （yiqie doushi zhon^ffianw~, occurs exactly in this way. With the evocation of this philosophical thought, the ＂intermediate object＂ （zhon^ianwto, we see the inseparable indigenous tie predestined between Lu Xun and Chinese traditional culture, even while he fiercely fights it. Lu Xun＇s innovative idea was produced in the process of deducing and developing the excellent and discarding the worthless in Chinese traditional culture, while absorbing and learning from the advanced thought of the West. Furthermore, his philosophy of the ＂intermediate object＂ forms the basis of his study and practice in translation. His purpose in translation is to bravely step out of the circle of inherent traditional culture, to come to the advanced ＂middle zone＂ where Chinese and Western cultures collide, and to probe into the new cultural factors from the West. In doing so he seeks to reform and improve Chinese traditional culture, and thus meet ＂the third era which China has never experienced before.＂ However, Lu Xun＇s idea of ＂intermediate objects＂ is neither the traditional idea of the ＂golden mean＂ （zhongyong zhidao） nor that of ＂hypocrisy＂ （xiangyuanyuan 乡愿）. Unfortunately they are often mixed together into chaos by many people. So it is necessary to have further discussion about these terms and distinguish them separately.
