Soybean(Glycine max[L.]Merr.)is a food and oil crop whose growth and yield are influenced by root and nodule development.In the present study,Gm NMHC5 was found to promote the formation of nodules in overexpressing mu...Soybean(Glycine max[L.]Merr.)is a food and oil crop whose growth and yield are influenced by root and nodule development.In the present study,Gm NMHC5 was found to promote the formation of nodules in overexpressing mutants.In contrast,the number of nodules in Gmnmhc5 edited with CRISPR/Cas9 decreased sharply.In 35 S:Gm NMHC5 mutants,expression levels of genes involved in nodulation were significantly up-regulated.Both in vitro and in vivo biochemical analyses showed that Gm NMHC5 directly interacted with Gm GAI(a DELLA protein),and the content of gibberellin 3(GA_(3))in overexpressing mutants was lower than that in the wild type.These results revealed that Gm NMHC5 participates in the classical GA signaling pathway,and may regulate the content of GA_3 to match the optimal concentration required for nodule formation,thereby promoting nodulation by directly interacting with Gm GAI.A model illustrating the mechanism by which Gm NMHC5 promotes soybean nodulation is presented.展开更多
Background:Cardiomyocytes derived from human embryonic stem cells(hESCs)are regulated by complex and stringent gene networks during differentiation.Long non-coding RNAs(lncRNAs)exert critical epigenetic regulatory fun...Background:Cardiomyocytes derived from human embryonic stem cells(hESCs)are regulated by complex and stringent gene networks during differentiation.Long non-coding RNAs(lncRNAs)exert critical epigenetic regulatory functions in multiple differentiation processes.However,the involvement of lncRNAs in the differentiation of hESCs into cardiomyocytes has not yet been fully elucidated.Here,we identified the key roles of ZFAS1(lncRNA zinc finger antisense 1)in the differentiation of cardiomyocytes from hESCs.Methods:A model of cardiomyocyte differentiation from stem cells was established using the monolayer differentiation method,and the number of beating hESCs-derived cardiomyocytes was calculated.Gene expression was analyzed by quantitative real-time PCR(qRTPCR).Immunofluorescence assays were performed to assess the expression of cardiac troponin T(cTnT)andα-actinin protein in cardiomyocytes.Results:qRT-PCR showed that ZFAS1 expression in the mesoderm was significantly higher than that in embryonic stem cells,cardiac progenitor cells,and cardiomyocytes.Knockdown of ZFAS1 inhibited cardiomyocyte differentiation from hESCs,which was characterized by reduced expression of the cardiac-specific markers cTnT,α-actinin,myosin heavy chain 6(MYH6),and myosin heavy chain 7(MYH7).In contrast,ZFAS1 overexpression remarkably increased the percentage of spontaneously beating cardiomyocytes.In terms of the mechanism,we found that ZFAS1 is an antisense lncRNA at the 5′end of the protein-coding gene ZNFX1.Knockdown of ZFAS1 could increase the mRNA expression level of ZNFX1.Furthermore,qRT-PCR demonstrated that the silencing of ZNFX1 led to an increase in cardiac-specific markers that predicted the promotion of cardiomyocyte differentiation.Conclusion:Altogether,these data suggest that lncRNA-ZFAS1 is required for cardiac differentiation by functionally inhibiting the expression of ZNFX1,which may provide a reference for the treatment of heart disease to a certain extent.展开更多
In order to improve the agricultural eco-efficiency and promote the sustainable development of agriculture in Henan Province, China, based on the footprint theory, the super-efficiency SBM model </span></span...In order to improve the agricultural eco-efficiency and promote the sustainable development of agriculture in Henan Province, China, based on the footprint theory, the super-efficiency SBM model </span></span><span><span><span style="font-family:"">is</span></span></span><span><span><span style="font-family:""> used to scientifically calculate and analyze the agricultural eco-efficiency in Henan Province. On this basis, the influencing factors of agricultural eco-efficiency in Henan Province are quantitatively analyzed by using the grey incidence analysis model. The <span>results s</span><span>how that unilaterally considering one of grey water footprint</span></span></span></span><span><span><span style="font-family:"">s</span></span></span><span><span><span style="font-family:""> and carbon footprint</span></span></span><span><span><span style="font-family:"">s</span></span></span><span><span><span style="font-family:""> will overestimate or underestimate the agricultural eco-efficiency of Henan Province in different degrees in different time periods, and the agricultural eco-efficiency obtained by comprehensively considering grey water footprint and carbon footprint (GWCAEE) is more in line with the reality of agricultural development in Henan Province. In 2000-2004, GWCAEE in Henan Province was better. During 2005-2014, GWCAEE in Henan Province showed a fluctuating decline and continued to be in an inefficient state. From 2015</span></span></span><span><span><span style="font-family:""> to 2019, GWCAEE of Henan Province gradually increased, and it became effective in 2019. In recent years, GWCAEE has developed well. Through the grey incidence analysis between 12 influencing factors including endogenous factors and exogenous factors and GWCAEE, it is found that the six leading factors of GWCAEE in Henan Province are agricultural structure, financial input for agriculture, number of agricultural employees, crop sown area, consumption of chemical pesticide, consumption of agricultural diesel oil. According to the above research conclusions, suggestions for improving agricultural eco-efficiency in Henan Province are put forward.展开更多
Photobiomodulation(PBM)has been shown to delay the pathological process of heart failure,but the exact mechanism of action is not clear.Mitochondria occupy one-third of the volume of mammalian cardiomyocytes(CMs)and a...Photobiomodulation(PBM)has been shown to delay the pathological process of heart failure,but the exact mechanism of action is not clear.Mitochondria occupy one-third of the volume of mammalian cardiomyocytes(CMs)and are central transport stations for CM energy metabolism.Therefore,in this study,we explored the regulatory effects of 630 nm light-emitting diodes(LED-Red)on the mitochondria of CMs.The results show that LED-Red-based PBM promotes adenosine triphosphate(ATP)synthesis by upregulating the expression of glycolipid metabolizing enzymes.Correspondingly,there was an improvement in the activity of succinate dehydrogenase(SDH),a key enzyme in the mitochondrial electron transport chain,and the mitochondrial membrane potential.Meanwhile,LED-Red affected the state of mitochondrial oxidative stress and promoted the generation of reactive oxygen species(ROS),but the increased ROS production did not damage the CMs.In addition,mitochondrial division and fusion were also affected by the stimulation of LED-Red.Finally,PBM treatment led to a significant increase in transcript levels of mitochondrial transcription factor A(TFAM),which controls the stability of the mitochondrial genome.Collectively,irradiation with LEDs at 630 nm played a regulatory role in mitochondrial function,suggesting that mitochondria appear to be the recipients of PBM treatment.This study provides more insights into the mechanisms underlying PBM treatment in heart diseases.展开更多
Objective:Long noncoding RNAs(lncRNAs)play an important role in regulating the occurrence and development of cardiovascular diseases.However,the role of lncRNAs in heart aging remains poorly understood.The objective o...Objective:Long noncoding RNAs(lncRNAs)play an important role in regulating the occurrence and development of cardiovascular diseases.However,the role of lncRNAs in heart aging remains poorly understood.The objective of this study was to identify differentially expressed lncRNAs in the heart of aging mice and elucidate the relevant regulatory pathways of cardiac aging.Materials and methods:Echocardiography was used to detect the cardiac function of 18-months(aged)and 3-months(young)old C57BL/6 mice.Microarray analysis was performed to unravel the expression profiles of lncRNAs and mRNAs,and qRT-PCR to verify the highly dysregulated lncRNAs.Results:Our results demonstrated that the heart function in aged mice was impaired relative to young ones.Microarray results showed that 155 lncRNAs were upregulated and 37 were downregulated,and 170 mRNAs were significantly upregulated and 44 were remarkably downregulated in aging hearts.Gene ontology analysis indicated that differentially expressed genes are mainly related to immune function,cell proliferation,copper ion response,and cellular cation homeostasis.KEGG pathway analysis showed that the differentially expressed mRNAs are related to cytokine-cytokine receptor interaction,inflammatory mediator regulation of TRP channels,and the NF-kappa B signaling pathway.Conclusion:These results imply that the differentially expressed lncRNAs may regulate the development of heart aging.This study provides a new perspective on the potential effects and mechanisms of lncRNAs in heart aging.展开更多
In a fast-paced career,employees are facing more and more pressure,and job burnout caused by long-term work pressure has become the main reason for many employees to leave.If the job burnout can’t be effectively reso...In a fast-paced career,employees are facing more and more pressure,and job burnout caused by long-term work pressure has become the main reason for many employees to leave.If the job burnout can’t be effectively resolved,it will have a great impact on personal future development and the good running of the enterprise.Starting from the perspective of organizational psychology,this study investigates 200 employees on their psychological contracts and job burnouts to discover the relationship between them that people are most concerned about at the moment,so as to propose some reasonable suggestions to improve the employee job burnout and enhance the mutual identification between employee and the company.The research results show that job burnout and psychological contract show a critically negative correlation;the stronger the psychological contract,the lower the probability of employee job burnout.In addition,the establishment of a strong psychological contract between the company and the employee can effectively reduce the employee’s job burnout,thereby ensuring the mutual benefit of the employee and the company.展开更多
Dear Editor,Myocardial infarction(MI)is characterized by the loss of functional cardiomyocyte(CM)in the heart,resulting in cardiac systolic dysfunction and heart failure.1,2 Increasing evidence suggested that in the h...Dear Editor,Myocardial infarction(MI)is characterized by the loss of functional cardiomyocyte(CM)in the heart,resulting in cardiac systolic dysfunction and heart failure.1,2 Increasing evidence suggested that in the heart of neonatal mice after apical resection(AR),the CM can proliferate and regenerate myocardium to repair the heart.While in the heart of adult mice after MI,the CM loses the ability to re-enter the cell cycle but undergoes hypertrophic growth.展开更多
基金supported by the National Natural Science Foundation of China(31271636)the China Agriculture Research System(CARS-04)。
文摘Soybean(Glycine max[L.]Merr.)is a food and oil crop whose growth and yield are influenced by root and nodule development.In the present study,Gm NMHC5 was found to promote the formation of nodules in overexpressing mutants.In contrast,the number of nodules in Gmnmhc5 edited with CRISPR/Cas9 decreased sharply.In 35 S:Gm NMHC5 mutants,expression levels of genes involved in nodulation were significantly up-regulated.Both in vitro and in vivo biochemical analyses showed that Gm NMHC5 directly interacted with Gm GAI(a DELLA protein),and the content of gibberellin 3(GA_(3))in overexpressing mutants was lower than that in the wild type.These results revealed that Gm NMHC5 participates in the classical GA signaling pathway,and may regulate the content of GA_3 to match the optimal concentration required for nodule formation,thereby promoting nodulation by directly interacting with Gm GAI.A model illustrating the mechanism by which Gm NMHC5 promotes soybean nodulation is presented.
基金the National Natural Science Foundation of China[81573434 to BZC]Heilongjiang Touyan Innovation Team Program[BZC],HMU Marshal Initiative Funding(HMUMIF-21018 to BZC)National Nature Science Youth Foudation of China[82000226 to XFG].
文摘Background:Cardiomyocytes derived from human embryonic stem cells(hESCs)are regulated by complex and stringent gene networks during differentiation.Long non-coding RNAs(lncRNAs)exert critical epigenetic regulatory functions in multiple differentiation processes.However,the involvement of lncRNAs in the differentiation of hESCs into cardiomyocytes has not yet been fully elucidated.Here,we identified the key roles of ZFAS1(lncRNA zinc finger antisense 1)in the differentiation of cardiomyocytes from hESCs.Methods:A model of cardiomyocyte differentiation from stem cells was established using the monolayer differentiation method,and the number of beating hESCs-derived cardiomyocytes was calculated.Gene expression was analyzed by quantitative real-time PCR(qRTPCR).Immunofluorescence assays were performed to assess the expression of cardiac troponin T(cTnT)andα-actinin protein in cardiomyocytes.Results:qRT-PCR showed that ZFAS1 expression in the mesoderm was significantly higher than that in embryonic stem cells,cardiac progenitor cells,and cardiomyocytes.Knockdown of ZFAS1 inhibited cardiomyocyte differentiation from hESCs,which was characterized by reduced expression of the cardiac-specific markers cTnT,α-actinin,myosin heavy chain 6(MYH6),and myosin heavy chain 7(MYH7).In contrast,ZFAS1 overexpression remarkably increased the percentage of spontaneously beating cardiomyocytes.In terms of the mechanism,we found that ZFAS1 is an antisense lncRNA at the 5′end of the protein-coding gene ZNFX1.Knockdown of ZFAS1 could increase the mRNA expression level of ZNFX1.Furthermore,qRT-PCR demonstrated that the silencing of ZNFX1 led to an increase in cardiac-specific markers that predicted the promotion of cardiomyocyte differentiation.Conclusion:Altogether,these data suggest that lncRNA-ZFAS1 is required for cardiac differentiation by functionally inhibiting the expression of ZNFX1,which may provide a reference for the treatment of heart disease to a certain extent.
文摘In order to improve the agricultural eco-efficiency and promote the sustainable development of agriculture in Henan Province, China, based on the footprint theory, the super-efficiency SBM model </span></span><span><span><span style="font-family:"">is</span></span></span><span><span><span style="font-family:""> used to scientifically calculate and analyze the agricultural eco-efficiency in Henan Province. On this basis, the influencing factors of agricultural eco-efficiency in Henan Province are quantitatively analyzed by using the grey incidence analysis model. The <span>results s</span><span>how that unilaterally considering one of grey water footprint</span></span></span></span><span><span><span style="font-family:"">s</span></span></span><span><span><span style="font-family:""> and carbon footprint</span></span></span><span><span><span style="font-family:"">s</span></span></span><span><span><span style="font-family:""> will overestimate or underestimate the agricultural eco-efficiency of Henan Province in different degrees in different time periods, and the agricultural eco-efficiency obtained by comprehensively considering grey water footprint and carbon footprint (GWCAEE) is more in line with the reality of agricultural development in Henan Province. In 2000-2004, GWCAEE in Henan Province was better. During 2005-2014, GWCAEE in Henan Province showed a fluctuating decline and continued to be in an inefficient state. From 2015</span></span></span><span><span><span style="font-family:""> to 2019, GWCAEE of Henan Province gradually increased, and it became effective in 2019. In recent years, GWCAEE has developed well. Through the grey incidence analysis between 12 influencing factors including endogenous factors and exogenous factors and GWCAEE, it is found that the six leading factors of GWCAEE in Henan Province are agricultural structure, financial input for agriculture, number of agricultural employees, crop sown area, consumption of chemical pesticide, consumption of agricultural diesel oil. According to the above research conclusions, suggestions for improving agricultural eco-efficiency in Henan Province are put forward.
基金This work was supported by grants from the National Key Research and Development Program of China(2017YFB0403802).
文摘Photobiomodulation(PBM)has been shown to delay the pathological process of heart failure,but the exact mechanism of action is not clear.Mitochondria occupy one-third of the volume of mammalian cardiomyocytes(CMs)and are central transport stations for CM energy metabolism.Therefore,in this study,we explored the regulatory effects of 630 nm light-emitting diodes(LED-Red)on the mitochondria of CMs.The results show that LED-Red-based PBM promotes adenosine triphosphate(ATP)synthesis by upregulating the expression of glycolipid metabolizing enzymes.Correspondingly,there was an improvement in the activity of succinate dehydrogenase(SDH),a key enzyme in the mitochondrial electron transport chain,and the mitochondrial membrane potential.Meanwhile,LED-Red affected the state of mitochondrial oxidative stress and promoted the generation of reactive oxygen species(ROS),but the increased ROS production did not damage the CMs.In addition,mitochondrial division and fusion were also affected by the stimulation of LED-Red.Finally,PBM treatment led to a significant increase in transcript levels of mitochondrial transcription factor A(TFAM),which controls the stability of the mitochondrial genome.Collectively,irradiation with LEDs at 630 nm played a regulatory role in mitochondrial function,suggesting that mitochondria appear to be the recipients of PBM treatment.This study provides more insights into the mechanisms underlying PBM treatment in heart diseases.
基金This work was supported by the National Natural Science Fund of China(81573434/81872857)the Program for New Century Excellent Talents in Heilongjiang Provincial University(grant number 1252-NCET-013).
文摘Objective:Long noncoding RNAs(lncRNAs)play an important role in regulating the occurrence and development of cardiovascular diseases.However,the role of lncRNAs in heart aging remains poorly understood.The objective of this study was to identify differentially expressed lncRNAs in the heart of aging mice and elucidate the relevant regulatory pathways of cardiac aging.Materials and methods:Echocardiography was used to detect the cardiac function of 18-months(aged)and 3-months(young)old C57BL/6 mice.Microarray analysis was performed to unravel the expression profiles of lncRNAs and mRNAs,and qRT-PCR to verify the highly dysregulated lncRNAs.Results:Our results demonstrated that the heart function in aged mice was impaired relative to young ones.Microarray results showed that 155 lncRNAs were upregulated and 37 were downregulated,and 170 mRNAs were significantly upregulated and 44 were remarkably downregulated in aging hearts.Gene ontology analysis indicated that differentially expressed genes are mainly related to immune function,cell proliferation,copper ion response,and cellular cation homeostasis.KEGG pathway analysis showed that the differentially expressed mRNAs are related to cytokine-cytokine receptor interaction,inflammatory mediator regulation of TRP channels,and the NF-kappa B signaling pathway.Conclusion:These results imply that the differentially expressed lncRNAs may regulate the development of heart aging.This study provides a new perspective on the potential effects and mechanisms of lncRNAs in heart aging.
文摘In a fast-paced career,employees are facing more and more pressure,and job burnout caused by long-term work pressure has become the main reason for many employees to leave.If the job burnout can’t be effectively resolved,it will have a great impact on personal future development and the good running of the enterprise.Starting from the perspective of organizational psychology,this study investigates 200 employees on their psychological contracts and job burnouts to discover the relationship between them that people are most concerned about at the moment,so as to propose some reasonable suggestions to improve the employee job burnout and enhance the mutual identification between employee and the company.The research results show that job burnout and psychological contract show a critically negative correlation;the stronger the psychological contract,the lower the probability of employee job burnout.In addition,the establishment of a strong psychological contract between the company and the employee can effectively reduce the employee’s job burnout,thereby ensuring the mutual benefit of the employee and the company.
基金This work was supported by the National Nature Science Foundation of China[92168119/81872857/82100300]Heilongjiang Touyan Innovation Team Program[B.Z.C.],and HMU Marshal Initiative Funding(HMUMIF-21018).
文摘Dear Editor,Myocardial infarction(MI)is characterized by the loss of functional cardiomyocyte(CM)in the heart,resulting in cardiac systolic dysfunction and heart failure.1,2 Increasing evidence suggested that in the heart of neonatal mice after apical resection(AR),the CM can proliferate and regenerate myocardium to repair the heart.While in the heart of adult mice after MI,the CM loses the ability to re-enter the cell cycle but undergoes hypertrophic growth.
