Zinc binding group(ZBG)is the crucial moiety in the chemical structure of any HDAC inhibitor.In the present study,a series of sulphur-containing ZBG were designed and synthesized in the novel HDAC inhibitors to replac...Zinc binding group(ZBG)is the crucial moiety in the chemical structure of any HDAC inhibitor.In the present study,a series of sulphur-containing ZBG were designed and synthesized in the novel HDAC inhibitors to replace the classical ZBGs of SAHA and BML-210,hydroxamic acids and benzamides,respectively.The HDAC inhibitory activity and the structure-activity relationships of these molecules were analyzed.A sulphur-rich group,diethylcarbamo(dithioperoxo)thioate,was finally identified as a novel potent ZBG.Among all the synthesized compounds,4 d was much more potent compared with BML-210,and it showed similar inhibitory effect of SAHA against HDAC isoforms 1 and 2.Therefore,it was chosen as a lead compound.展开更多
Beam steering technology,with its flexible directionality,holds significant application value in freespace optical communication,LiDAR,advanced manufacturing,medical laser surgery,and so on.However,conventional method...Beam steering technology,with its flexible directionality,holds significant application value in freespace optical communication,LiDAR,advanced manufacturing,medical laser surgery,and so on.However,conventional methods achieving beam steering face various challenges:mechanical regimes,fixed operating wavelengths,and—specifically—a lack of tunable light sources in the blue-green spectral band(450 to 550 nm),which is recognized as the optimal transmission window for underwater wireless optical communication(UWOC)and the most efficient wavelength band for metal and battery manufacturing.With the aid of broadband feedback of Rayleigh backscattering and the acousto-optic modulation device-induced wavelength selectivity,we demonstrate,to the best of our knowledge,the first continuously wavelength-swept random fiber laser,which operates across the 1040 to 1090 nm spectral range,featuring a simple architecture,stable temporal output,and electrically controlled spectral sweeping properties.By employing a chirped periodically poled lithium niobate crystal for broadband phase matching,we extend the operational wavelength to the green band through second-harmonic generation.Furthermore,spectral-to-spatial mapping of this wavelength-swept green random laser enables beam steering,achieving an angular range of~1.8 deg and a minimum angular resolution of~0.021 deg.We anticipate that this wavelength-swept green random laser and its empowered beam steering technology will benefit applications such as UWOC and advanced manufacturing.展开更多
Metabolite biosynthesis is regulated by gene expression,which is altered by DNA methylation in the promoter region.Chalcone isomerase(CHI)gene encodes a key enzyme in the Lithocarpus polystachyus Rehd flavonoid pathwa...Metabolite biosynthesis is regulated by gene expression,which is altered by DNA methylation in the promoter region.Chalcone isomerase(CHI)gene encodes a key enzyme in the Lithocarpus polystachyus Rehd flavonoid pathway,and the expression of L.polystachyus CHI(LpCHI)is closely related to the synthesis of flavonoid metabolites.In this study,we analyzed the DNA methylation site of the LpCHI promoter and its effect on gene expression and metabolite accumulation.The proportions of three types of LpCHI promoter DNA methylation are 7.5%,68.75%,18.75%,determined by bisulfite sequencing.Transcriptome sequencing shows that LpCHI is strongly up-regulated in LpCHI promoter methylation Type A but down-regulated in LpCHI promoter methylation Type B and Type C.The expression of LpCHI shows no significant difference between Type B and Type C.Moreover,nine kinds of differentially expressed transcription factors(DETFs)bind to seven CpG-sites of the LpCHI promoter region to regulate LpCHI expression.The results of metabolomics show that differentially accumulated flavonoids are higher in LpCHI promoter methylation Type A than in LpCHI promoter methylation Type B and Type C.Additionally,a positive correlation was found between the LpCHI expression and flavonoids accumulation.These results show that the effect of CpG site-specificity on gene transcription is great than that of overall promoter DNA methylation on gene transcription.The mechanisms of flavonoid genes regulating metabolite accumulation are further revealed.展开更多
Spontaneous Raman emission(SpRE),traditionally viewed as a detrimental effect,impairing spectral purity and imposing fundamental limits on power scaling in conventional fiber lasers,is being harnessed as a novel mecha...Spontaneous Raman emission(SpRE),traditionally viewed as a detrimental effect,impairing spectral purity and imposing fundamental limits on power scaling in conventional fiber lasers,is being harnessed as a novel mechanism to realize high-power incoherent light sources.Compared to previous methods that rely on rare-earth-doped amplified spontaneous emission(ASE),this approach offers superior cost-effectiveness and inherent wavelength flexibility,enabling access to unconventional spectral regions critical for emerging applications like photonic computing and optical coherence tomography(OCT).As a demonstration,we first generate SpRE in a passive fiber segment,and then efficiently amplify this incoherent seed via stimulated Raman scattering to achieve highpower output.The resulting fiber source operates at 1116 nm with 23.03 W output power,8.24 nm 3 dB bandwidth,and 60.6%optical-to-optical efficiency while exhibiting high temporal stability.The incoherent nature eliminates interference artifacts and makes this source highly favorable for applications such as laser-driven inertial confinement fusion,photonic computing,and OCT.展开更多
基金National Natural Science Foundation of China(Grant No.81573272)
文摘Zinc binding group(ZBG)is the crucial moiety in the chemical structure of any HDAC inhibitor.In the present study,a series of sulphur-containing ZBG were designed and synthesized in the novel HDAC inhibitors to replace the classical ZBGs of SAHA and BML-210,hydroxamic acids and benzamides,respectively.The HDAC inhibitory activity and the structure-activity relationships of these molecules were analyzed.A sulphur-rich group,diethylcarbamo(dithioperoxo)thioate,was finally identified as a novel potent ZBG.Among all the synthesized compounds,4 d was much more potent compared with BML-210,and it showed similar inhibitory effect of SAHA against HDAC isoforms 1 and 2.Therefore,it was chosen as a lead compound.
基金supported by the National Natural Science Foundation of China(Grant No.62305391)the NUDT Research Program(Grant No.ZK 23-24)the Scientific Fund of National University of Defense Technology(Grant No.24-09)。
文摘Beam steering technology,with its flexible directionality,holds significant application value in freespace optical communication,LiDAR,advanced manufacturing,medical laser surgery,and so on.However,conventional methods achieving beam steering face various challenges:mechanical regimes,fixed operating wavelengths,and—specifically—a lack of tunable light sources in the blue-green spectral band(450 to 550 nm),which is recognized as the optimal transmission window for underwater wireless optical communication(UWOC)and the most efficient wavelength band for metal and battery manufacturing.With the aid of broadband feedback of Rayleigh backscattering and the acousto-optic modulation device-induced wavelength selectivity,we demonstrate,to the best of our knowledge,the first continuously wavelength-swept random fiber laser,which operates across the 1040 to 1090 nm spectral range,featuring a simple architecture,stable temporal output,and electrically controlled spectral sweeping properties.By employing a chirped periodically poled lithium niobate crystal for broadband phase matching,we extend the operational wavelength to the green band through second-harmonic generation.Furthermore,spectral-to-spatial mapping of this wavelength-swept green random laser enables beam steering,achieving an angular range of~1.8 deg and a minimum angular resolution of~0.021 deg.We anticipate that this wavelength-swept green random laser and its empowered beam steering technology will benefit applications such as UWOC and advanced manufacturing.
基金supported by the Natural Science Foundation of Hebei Province(H2020209033)the Science and Technology Project of Hebei Education Department(ZD2019075).
文摘Metabolite biosynthesis is regulated by gene expression,which is altered by DNA methylation in the promoter region.Chalcone isomerase(CHI)gene encodes a key enzyme in the Lithocarpus polystachyus Rehd flavonoid pathway,and the expression of L.polystachyus CHI(LpCHI)is closely related to the synthesis of flavonoid metabolites.In this study,we analyzed the DNA methylation site of the LpCHI promoter and its effect on gene expression and metabolite accumulation.The proportions of three types of LpCHI promoter DNA methylation are 7.5%,68.75%,18.75%,determined by bisulfite sequencing.Transcriptome sequencing shows that LpCHI is strongly up-regulated in LpCHI promoter methylation Type A but down-regulated in LpCHI promoter methylation Type B and Type C.The expression of LpCHI shows no significant difference between Type B and Type C.Moreover,nine kinds of differentially expressed transcription factors(DETFs)bind to seven CpG-sites of the LpCHI promoter region to regulate LpCHI expression.The results of metabolomics show that differentially accumulated flavonoids are higher in LpCHI promoter methylation Type A than in LpCHI promoter methylation Type B and Type C.Additionally,a positive correlation was found between the LpCHI expression and flavonoids accumulation.These results show that the effect of CpG site-specificity on gene transcription is great than that of overall promoter DNA methylation on gene transcription.The mechanisms of flavonoid genes regulating metabolite accumulation are further revealed.
基金National Natural Science Foundation of China(62305391)Scientific Fund of National University of Defense Technology(25-02,ZK 23-24)。
文摘Spontaneous Raman emission(SpRE),traditionally viewed as a detrimental effect,impairing spectral purity and imposing fundamental limits on power scaling in conventional fiber lasers,is being harnessed as a novel mechanism to realize high-power incoherent light sources.Compared to previous methods that rely on rare-earth-doped amplified spontaneous emission(ASE),this approach offers superior cost-effectiveness and inherent wavelength flexibility,enabling access to unconventional spectral regions critical for emerging applications like photonic computing and optical coherence tomography(OCT).As a demonstration,we first generate SpRE in a passive fiber segment,and then efficiently amplify this incoherent seed via stimulated Raman scattering to achieve highpower output.The resulting fiber source operates at 1116 nm with 23.03 W output power,8.24 nm 3 dB bandwidth,and 60.6%optical-to-optical efficiency while exhibiting high temporal stability.The incoherent nature eliminates interference artifacts and makes this source highly favorable for applications such as laser-driven inertial confinement fusion,photonic computing,and OCT.
