OBJECTIVE:To elucidate the protective effect of Qingdai(Indigo Naturalis,QD)on ulcerative colitis(UC)by means of in silico and in vivo approaches.METHODS:A systems pharmacology analysis was performed to predict the ac...OBJECTIVE:To elucidate the protective effect of Qingdai(Indigo Naturalis,QD)on ulcerative colitis(UC)by means of in silico and in vivo approaches.METHODS:A systems pharmacology analysis was performed to predict the active components of QD whereas the putative biological targets of QD against UC were obtained through target fishing,network cons-truction and enrichment analyses.Meanwhile,we examined the ameliorative effect of QD in a mouse model of dextran sulfate sodium(DSS)-induced colitis.During the 10-day experiment,the control and diseased mice were given with oral gavages of QD(1.3 g raw herbs·kg^(-1)·d^(-1))or 5-aminosalicylic acid(5-ASA,100 mg·kg^(-1)·d^(-1))every day.The underlying pharma-cological mechanisms of QD in UC were determined using polymerase chain reaction tests,histological staining,enzyme-linked immunoassays,and Western blotting analysis.RESULTS:Searching from various network pharmacology databases,29 compounds were identified in QD.According to the screening criteria suggested by TCMSP(i.e.OB≥30%and DL≥0.18),nine of them were considered the active ingredients that contribute to the ameliorative effects of QD on different mouse models of colitis.Most importantly,the protective effect of QD on DSS-induced colitis was significantly associated with modulations of the expression levels of glycogen synthase kinase 3-β(Gsk3-β)and forkhead box p3(Foxp3),which are widely considered as important regulators of excessive inflammatory responses.CONCLUSIONS:The results of this study provide solid scientific evidence for the use of QD or its core active components in the clinical management of UC.展开更多
目的比较右美托咪定与咪达唑仑在腹腔镜手术中的麻醉效果。方法选取2017年1月-2019年1月于广东省清远市清新区人民医院行腹腔镜手术治疗的患者50例,采用随机数字表法分为右美托咪定组和咪达唑仑组,每组25例。麻醉诱导前10 min右美托咪...目的比较右美托咪定与咪达唑仑在腹腔镜手术中的麻醉效果。方法选取2017年1月-2019年1月于广东省清远市清新区人民医院行腹腔镜手术治疗的患者50例,采用随机数字表法分为右美托咪定组和咪达唑仑组,每组25例。麻醉诱导前10 min右美托咪定组给予右美托咪定静脉泵注,咪达唑仑组一次性给予咪达唑仑静脉注射,2组均予芬太尼、丙泊酚、顺阿曲库铵完成麻醉诱导插管,术中麻醉维持吸入七氟烷,根据血压、麻醉深度监测情况追加芬太尼。比较麻醉前(T0)、麻醉后10 min(T1)、插管1 min后(T2)、手术气腹时(T3)、拔管后(T4)2组心率(HR)、平均动脉压(MAP)、术中芬太尼追加次数、拔管时间、拔管后15 min OAA/S镇静评分、视觉模拟评分法(VAS)评分。结果与T0比较,右美托咪定组T1时HR明显下降(P<0.05),咪达唑仑组T2、T3、T4时HR、MAP明显上升(P<0.05);T1、T2、T3、T4时右美托咪定组HR明显低于咪达唑仑组(P<0.05)。2组均无术中知晓。右美托咪定组芬太尼追加次数少于咪达唑仑组,术后拔管时间短于咪达唑仑组,拔管后15 min OAA/S镇静评分高于咪达唑仑组,VAS评分低于咪达唑仑组(P均<0.05)。结论与咪达唑仑比较,右美托咪定在腹腔镜手术麻醉中具有血流动力学稳定、减少麻醉用药剂量、苏醒快、苏醒质量高等优点。展开更多
基金Supported by Natural Science Foundation of Guangdong Province:Mechanism of Chang-An Decotion in Neuropeptide Spexin related GSK-3βRegulating Intestinal Nerve Immune Network in Ulcerative Colitis(No.2018A030310614)National Natural Science Foundation of China:Mchanism of Chang-An Decotion in Intestinal Mucosal Immunity of Ulcerative Colitis on Exocrine Mediated Rab27(No.81903963)Department of Education of Guangdong Province Project:Mchanism of Chang-An decotion of Ulcerative Colitis on Exocrine Mediated GSK-3βRegulating Th17/Treg in Ulcerative Colitis(No.2017KQNCX045)。
文摘OBJECTIVE:To elucidate the protective effect of Qingdai(Indigo Naturalis,QD)on ulcerative colitis(UC)by means of in silico and in vivo approaches.METHODS:A systems pharmacology analysis was performed to predict the active components of QD whereas the putative biological targets of QD against UC were obtained through target fishing,network cons-truction and enrichment analyses.Meanwhile,we examined the ameliorative effect of QD in a mouse model of dextran sulfate sodium(DSS)-induced colitis.During the 10-day experiment,the control and diseased mice were given with oral gavages of QD(1.3 g raw herbs·kg^(-1)·d^(-1))or 5-aminosalicylic acid(5-ASA,100 mg·kg^(-1)·d^(-1))every day.The underlying pharma-cological mechanisms of QD in UC were determined using polymerase chain reaction tests,histological staining,enzyme-linked immunoassays,and Western blotting analysis.RESULTS:Searching from various network pharmacology databases,29 compounds were identified in QD.According to the screening criteria suggested by TCMSP(i.e.OB≥30%and DL≥0.18),nine of them were considered the active ingredients that contribute to the ameliorative effects of QD on different mouse models of colitis.Most importantly,the protective effect of QD on DSS-induced colitis was significantly associated with modulations of the expression levels of glycogen synthase kinase 3-β(Gsk3-β)and forkhead box p3(Foxp3),which are widely considered as important regulators of excessive inflammatory responses.CONCLUSIONS:The results of this study provide solid scientific evidence for the use of QD or its core active components in the clinical management of UC.
文摘目的比较右美托咪定与咪达唑仑在腹腔镜手术中的麻醉效果。方法选取2017年1月-2019年1月于广东省清远市清新区人民医院行腹腔镜手术治疗的患者50例,采用随机数字表法分为右美托咪定组和咪达唑仑组,每组25例。麻醉诱导前10 min右美托咪定组给予右美托咪定静脉泵注,咪达唑仑组一次性给予咪达唑仑静脉注射,2组均予芬太尼、丙泊酚、顺阿曲库铵完成麻醉诱导插管,术中麻醉维持吸入七氟烷,根据血压、麻醉深度监测情况追加芬太尼。比较麻醉前(T0)、麻醉后10 min(T1)、插管1 min后(T2)、手术气腹时(T3)、拔管后(T4)2组心率(HR)、平均动脉压(MAP)、术中芬太尼追加次数、拔管时间、拔管后15 min OAA/S镇静评分、视觉模拟评分法(VAS)评分。结果与T0比较,右美托咪定组T1时HR明显下降(P<0.05),咪达唑仑组T2、T3、T4时HR、MAP明显上升(P<0.05);T1、T2、T3、T4时右美托咪定组HR明显低于咪达唑仑组(P<0.05)。2组均无术中知晓。右美托咪定组芬太尼追加次数少于咪达唑仑组,术后拔管时间短于咪达唑仑组,拔管后15 min OAA/S镇静评分高于咪达唑仑组,VAS评分低于咪达唑仑组(P均<0.05)。结论与咪达唑仑比较,右美托咪定在腹腔镜手术麻醉中具有血流动力学稳定、减少麻醉用药剂量、苏醒快、苏醒质量高等优点。
