目的基于网络药理学和分子对接技术探究苍耳子散(CRZS)治疗异质性鼻炎的作用机制以及“多成分-多靶点-多通路”的整体药理作用特征。方法利用中药系统药理学数据库与分析平台(TCMSP)检索CRZS的活性成分及靶点;从GeneCards、DisGeNET、Ph...目的基于网络药理学和分子对接技术探究苍耳子散(CRZS)治疗异质性鼻炎的作用机制以及“多成分-多靶点-多通路”的整体药理作用特征。方法利用中药系统药理学数据库与分析平台(TCMSP)检索CRZS的活性成分及靶点;从GeneCards、DisGeNET、Phenopedia、TTD和PharmGKB数据库获取相关鼻炎的基因。通过STRING数据库与Cytoscape软件构建蛋白质-蛋白质相互作用(PPI)网络;利用STRING数据库对核心基因进行基因本体论(GO)富集分析和京都基因与基因组百科全书(KEGG)通路富集分析。通过Cytoscape软件构建“中药成分-靶点-信号通路”网络并筛选核心靶点;采用AutoDock软件对筛选得到的关键通路靶点进行分子对接。结果CRZS有131个核心基因,鼻炎相关靶点756个,交集靶点41个。GO生物富集条目2005条,KEGG通路富集条目151条,HIF-1、Lipid and atherosclerosis、Fluid shear stress and atherosclerosis等通路最具连接可能。分子对接显示,CEZS靶点的有效成分与3个核心基因连接紧密。结论CEZS可通过多成分、多靶点,多信号通路发挥治疗鼻炎的作用,其中最有可能通过HIF-1、lipid and atherosclerosis、Fluid shear stress and atherosclerosis等3条通路进行调控。展开更多
研究旨在降解酒精糟中几种毒素(呕吐毒素、伏马菌素、黄曲霉毒素),为酒精糟制成饲料奠定基础。利用实验得到的一株能够很好降解玉米酒糟毒素菌种酵母19-1,以酒精糟和麸皮为发酵底物进行固态发酵,通过正交试验对固态发酵过程中添加的营...研究旨在降解酒精糟中几种毒素(呕吐毒素、伏马菌素、黄曲霉毒素),为酒精糟制成饲料奠定基础。利用实验得到的一株能够很好降解玉米酒糟毒素菌种酵母19-1,以酒精糟和麸皮为发酵底物进行固态发酵,通过正交试验对固态发酵过程中添加的营养条件进行研究,测得发酵物毒素的含量,并通过重复最佳配方实验对结果进行验证。综合3种毒素测得情况,确定酵母19-1降解玉米酒精糟毒素的最优搭配组合为硫酸铵0%、水12 m L、硫酸镁0.015%、磷酸二氢钾0.05%、葡萄糖0%。展开更多
Sichuan Province has abundant leisure sports resources.An evaluation of the competitiveness of its leisure sports resources can provide a scientific basis for the selection and development of leisure sports industry c...Sichuan Province has abundant leisure sports resources.An evaluation of the competitiveness of its leisure sports resources can provide a scientific basis for the selection and development of leisure sports industry clusters in Sichuan Province.In addition,a competitiveness evaluation index system for leisure sports resources was constructed to assess the competitiveness of leisure sports resources for 21 regions in Sichuan Province.The results showed significant differences among the 21 regions,and they could be divided into three levels.Specifically,the first level included the seven regions of Chengdu,Leshan,Yibin,Mianyang,Zigong,Aba and Luzhou;the second level included the 11 regions of Ya’an,Guangyuan,Ganzi,Neijiang,Deyang,Guang’an,Liangshan,Nanchong,Panzhihua,Bazhong and Dazhou;and the third level included Meishan,Suining and Ziyang.In view of the resource conditions,environmental conditions,and development conditions,Chengdu,Leshan,Yibin,Mianyang,and Zigong were chosen as the most suitable areas for leisure sports industry clusters in Sichuan Province.Furthermore,suggestions for these five potential leisure sports industry clusters were proposed to promote the high-quality development of the sports industry in Sichuan Province.展开更多
文摘目的基于网络药理学和分子对接技术探究苍耳子散(CRZS)治疗异质性鼻炎的作用机制以及“多成分-多靶点-多通路”的整体药理作用特征。方法利用中药系统药理学数据库与分析平台(TCMSP)检索CRZS的活性成分及靶点;从GeneCards、DisGeNET、Phenopedia、TTD和PharmGKB数据库获取相关鼻炎的基因。通过STRING数据库与Cytoscape软件构建蛋白质-蛋白质相互作用(PPI)网络;利用STRING数据库对核心基因进行基因本体论(GO)富集分析和京都基因与基因组百科全书(KEGG)通路富集分析。通过Cytoscape软件构建“中药成分-靶点-信号通路”网络并筛选核心靶点;采用AutoDock软件对筛选得到的关键通路靶点进行分子对接。结果CRZS有131个核心基因,鼻炎相关靶点756个,交集靶点41个。GO生物富集条目2005条,KEGG通路富集条目151条,HIF-1、Lipid and atherosclerosis、Fluid shear stress and atherosclerosis等通路最具连接可能。分子对接显示,CEZS靶点的有效成分与3个核心基因连接紧密。结论CEZS可通过多成分、多靶点,多信号通路发挥治疗鼻炎的作用,其中最有可能通过HIF-1、lipid and atherosclerosis、Fluid shear stress and atherosclerosis等3条通路进行调控。
文摘研究旨在降解酒精糟中几种毒素(呕吐毒素、伏马菌素、黄曲霉毒素),为酒精糟制成饲料奠定基础。利用实验得到的一株能够很好降解玉米酒糟毒素菌种酵母19-1,以酒精糟和麸皮为发酵底物进行固态发酵,通过正交试验对固态发酵过程中添加的营养条件进行研究,测得发酵物毒素的含量,并通过重复最佳配方实验对结果进行验证。综合3种毒素测得情况,确定酵母19-1降解玉米酒精糟毒素的最优搭配组合为硫酸铵0%、水12 m L、硫酸镁0.015%、磷酸二氢钾0.05%、葡萄糖0%。
基金The Sichuan Tourism Development Research Center(LY24-03)。
文摘Sichuan Province has abundant leisure sports resources.An evaluation of the competitiveness of its leisure sports resources can provide a scientific basis for the selection and development of leisure sports industry clusters in Sichuan Province.In addition,a competitiveness evaluation index system for leisure sports resources was constructed to assess the competitiveness of leisure sports resources for 21 regions in Sichuan Province.The results showed significant differences among the 21 regions,and they could be divided into three levels.Specifically,the first level included the seven regions of Chengdu,Leshan,Yibin,Mianyang,Zigong,Aba and Luzhou;the second level included the 11 regions of Ya’an,Guangyuan,Ganzi,Neijiang,Deyang,Guang’an,Liangshan,Nanchong,Panzhihua,Bazhong and Dazhou;and the third level included Meishan,Suining and Ziyang.In view of the resource conditions,environmental conditions,and development conditions,Chengdu,Leshan,Yibin,Mianyang,and Zigong were chosen as the most suitable areas for leisure sports industry clusters in Sichuan Province.Furthermore,suggestions for these five potential leisure sports industry clusters were proposed to promote the high-quality development of the sports industry in Sichuan Province.
