In recent years,the demand for synchronous acquisition of three-dimensional(3D)shape and col-or texture has surged in fields such as cultural heritage preservation and healthcare.Addressing this need,this paper propos...In recent years,the demand for synchronous acquisition of three-dimensional(3D)shape and col-or texture has surged in fields such as cultural heritage preservation and healthcare.Addressing this need,this paper proposes a novel method for simultaneous 3D shape and color texture capture.First,a linear model correlating camera exposure time with grayscale values is established.Through exposure time calibration,the projected red,green and blue(RGB)light and white-light grayscale values captured by a monochrome cam-era are aligned.Then,three sets of color fringes are projected onto the object to identify optimal pixels for 3D reconstruction.And,three pure-color patterns are projected to synthesize the color texture.Experimental res-ults show that this method effectively achieves synchronous 3D shape and color texture acquisition,offering high speed and precision,and avoids color crosstalk interference common in 3D reconstruction of colored ob-jects using a monochrome camera.展开更多
为研究4型鸭疫里默氏杆菌(RA-4)对商品肉鸭的致病性及传播规律,本实验对疑似感染RA的病死鸭肝脏、脑组织进行细菌的分离培养与纯化,并进行生化试验、血清型鉴定,16S r RNA的PCR扩增及测序,采用K-B纸片法检测其药敏性。结果显示分离到1株...为研究4型鸭疫里默氏杆菌(RA-4)对商品肉鸭的致病性及传播规律,本实验对疑似感染RA的病死鸭肝脏、脑组织进行细菌的分离培养与纯化,并进行生化试验、血清型鉴定,16S r RNA的PCR扩增及测序,采用K-B纸片法检测其药敏性。结果显示分离到1株RA-4,该分离株符合RA的生化特征;PCR扩增及测序分析该分离株与RA16S r RNA基因的同源性在99.04%~99.47%;该分离株对恩诺沙星、多西环素、头孢吡肟等9种实验室常用药物均耐药,对氟苯尼考、大观霉素、头孢噻肟3种药物中介,表明该菌株为多重耐药菌株。将SPF番鸭分为5组(饮水感染组、空气感染组、肌肉注射组、接触组及对照组),每组采用相应的感染方式均感染0.5 m L/只分离菌液(1.0×10~7cfu/m L),其中接触组与肌肉注射组置于同一隔离器中,对照组单独饲养,感染后检测各组鸭的存活率,感染后不同时间采集各组鸭的喉头、泄殖腔拭子,采用PCR检测各组鸭的排毒情况,并剖检后观察各组鸭各脏器的剖检病变。结果显示,肌肉注射组鸭的存活率最低(30%),且显著低于其他4组(P<0.05);每组SPF鸭感染后的喉拭子均能检出RA,仅少部分泄殖腔拭子检出RA;各组鸭各脏器的剖检与组织病变观察结果显示,该分离株可引起鸭的死亡以及纤维素性心包炎和肝周炎等炎症性病变。上述结果表明,本研究从商品肉鸭中分离到1株RA-4,其对SPF鸭致病性较强,且其主要经呼吸道途径水平传播。本研究为临床感染RA-4的诊断和防控提供了参考依据。展开更多
文摘In recent years,the demand for synchronous acquisition of three-dimensional(3D)shape and col-or texture has surged in fields such as cultural heritage preservation and healthcare.Addressing this need,this paper proposes a novel method for simultaneous 3D shape and color texture capture.First,a linear model correlating camera exposure time with grayscale values is established.Through exposure time calibration,the projected red,green and blue(RGB)light and white-light grayscale values captured by a monochrome cam-era are aligned.Then,three sets of color fringes are projected onto the object to identify optimal pixels for 3D reconstruction.And,three pure-color patterns are projected to synthesize the color texture.Experimental res-ults show that this method effectively achieves synchronous 3D shape and color texture acquisition,offering high speed and precision,and avoids color crosstalk interference common in 3D reconstruction of colored ob-jects using a monochrome camera.
文摘为研究4型鸭疫里默氏杆菌(RA-4)对商品肉鸭的致病性及传播规律,本实验对疑似感染RA的病死鸭肝脏、脑组织进行细菌的分离培养与纯化,并进行生化试验、血清型鉴定,16S r RNA的PCR扩增及测序,采用K-B纸片法检测其药敏性。结果显示分离到1株RA-4,该分离株符合RA的生化特征;PCR扩增及测序分析该分离株与RA16S r RNA基因的同源性在99.04%~99.47%;该分离株对恩诺沙星、多西环素、头孢吡肟等9种实验室常用药物均耐药,对氟苯尼考、大观霉素、头孢噻肟3种药物中介,表明该菌株为多重耐药菌株。将SPF番鸭分为5组(饮水感染组、空气感染组、肌肉注射组、接触组及对照组),每组采用相应的感染方式均感染0.5 m L/只分离菌液(1.0×10~7cfu/m L),其中接触组与肌肉注射组置于同一隔离器中,对照组单独饲养,感染后检测各组鸭的存活率,感染后不同时间采集各组鸭的喉头、泄殖腔拭子,采用PCR检测各组鸭的排毒情况,并剖检后观察各组鸭各脏器的剖检病变。结果显示,肌肉注射组鸭的存活率最低(30%),且显著低于其他4组(P<0.05);每组SPF鸭感染后的喉拭子均能检出RA,仅少部分泄殖腔拭子检出RA;各组鸭各脏器的剖检与组织病变观察结果显示,该分离株可引起鸭的死亡以及纤维素性心包炎和肝周炎等炎症性病变。上述结果表明,本研究从商品肉鸭中分离到1株RA-4,其对SPF鸭致病性较强,且其主要经呼吸道途径水平传播。本研究为临床感染RA-4的诊断和防控提供了参考依据。
