Aim:Beta-adrenergic receptor blockers are conventionally used for the treatment of hypertension,tachycardia,and glaucoma.Research has shown that beta-blockers can accelerate wound epithelialization.In this study,we te...Aim:Beta-adrenergic receptor blockers are conventionally used for the treatment of hypertension,tachycardia,and glaucoma.Research has shown that beta-blockers can accelerate wound epithelialization.In this study,we tested the efficacy of the beta-blocker timolol in an ovine model of grafted full-thickness burn wound healing,which closely mimics clinical scenarios.Methods:Six full-thickness burn wounds were created on the sheep’s posterior surface.Twenty-four hours later,eschars were excised and meshed skin was grafted(Day0).The wounds in the treatment group received topical application of timolol.Blood flow was measured using a blood perfusion imager.Cardiovascular hemodynamics and blood glucose levels were recorded daily.The epithelialization rate on Day 14 was determined by planimetric assay and analyzed by paired t-test.The days that the epithelialization rate exceeded 85%,90%,and 95%were analyzed by survival analysis.To assess the potential influence of TGFβ,epithelial-mesenchymal transition(EMT),or myofibroblast activation(MFA)on wound healing,the RNA abundance of gene products related to these Results:The epithelialization rate on Day 14 was significantly higher in the treatment group.The days that the epithelialization rate exceeded 85%,90%,and 95%were significantly shorter in the treatment group.There was no significant difference in wound blood flow or RNA abundance related to TGFβ,EMT,or MFA-related pathways among the groups at any time point.Conclusion:The results demonstrate that the beta-blocker timolol accelerates epithelialization of mesh skin grafted full-thickness burn wounds through a mechanism other than improving wound blood flow.展开更多
基金support for Nakamoto K,Batsaikhan TA,Kakizaki R,Heathman T,and Enkhbaatar P was National Institutes of Health GM097480.
文摘Aim:Beta-adrenergic receptor blockers are conventionally used for the treatment of hypertension,tachycardia,and glaucoma.Research has shown that beta-blockers can accelerate wound epithelialization.In this study,we tested the efficacy of the beta-blocker timolol in an ovine model of grafted full-thickness burn wound healing,which closely mimics clinical scenarios.Methods:Six full-thickness burn wounds were created on the sheep’s posterior surface.Twenty-four hours later,eschars were excised and meshed skin was grafted(Day0).The wounds in the treatment group received topical application of timolol.Blood flow was measured using a blood perfusion imager.Cardiovascular hemodynamics and blood glucose levels were recorded daily.The epithelialization rate on Day 14 was determined by planimetric assay and analyzed by paired t-test.The days that the epithelialization rate exceeded 85%,90%,and 95%were analyzed by survival analysis.To assess the potential influence of TGFβ,epithelial-mesenchymal transition(EMT),or myofibroblast activation(MFA)on wound healing,the RNA abundance of gene products related to these Results:The epithelialization rate on Day 14 was significantly higher in the treatment group.The days that the epithelialization rate exceeded 85%,90%,and 95%were significantly shorter in the treatment group.There was no significant difference in wound blood flow or RNA abundance related to TGFβ,EMT,or MFA-related pathways among the groups at any time point.Conclusion:The results demonstrate that the beta-blocker timolol accelerates epithelialization of mesh skin grafted full-thickness burn wounds through a mechanism other than improving wound blood flow.
