Unlocking the full potential of genome editing for crop improvement has been hindered by inefficient reagent delivery methods and the reliance on tissue culture for generating gene-edited plants.In addition,convention...Unlocking the full potential of genome editing for crop improvement has been hindered by inefficient reagent delivery methods and the reliance on tissue culture for generating gene-edited plants.In addition,conventional transgene-mediated CRISPR/Cas delivery methods require a lengthy outcrossing process to eliminate CRISPR/Cas-associated sequences and produce transgene-free lines,circumventing regulatory restrictions related to transgenesis.RNA viral vectors offer a promising alternative for delivering genome engineering reagents,bypassing the need for tissue culture and avoiding transgenesis.Here,we discuss the bottlenecks in virus-mediated gene editing in plants,including virus exclusion from meristems,the requirement for a Cas-expressing plant,and concerns about virus host range and seed transmission.展开更多
基金supported by the Governor's University Research Initiative programfrom the state of Texas.
文摘Unlocking the full potential of genome editing for crop improvement has been hindered by inefficient reagent delivery methods and the reliance on tissue culture for generating gene-edited plants.In addition,conventional transgene-mediated CRISPR/Cas delivery methods require a lengthy outcrossing process to eliminate CRISPR/Cas-associated sequences and produce transgene-free lines,circumventing regulatory restrictions related to transgenesis.RNA viral vectors offer a promising alternative for delivering genome engineering reagents,bypassing the need for tissue culture and avoiding transgenesis.Here,we discuss the bottlenecks in virus-mediated gene editing in plants,including virus exclusion from meristems,the requirement for a Cas-expressing plant,and concerns about virus host range and seed transmission.
