Obesity is a complex metabolic disorder associated with chronic low-grade inflammation and gut microbiota dysbiosis.This study evaluated the therapeutic effects of morin,a natural flavonoid,in mitigating inflammation ...Obesity is a complex metabolic disorder associated with chronic low-grade inflammation and gut microbiota dysbiosis.This study evaluated the therapeutic effects of morin,a natural flavonoid,in mitigating inflammation and metabolic disturbances induced by a high-fat diet in male C57BL/6 mice.Mice were fed either a control diet or high-fat diet for 16 weeks,and after obesity was induced,a subset of high-fat diet fed mice received morin via oral gavage at a dose of 100 mg/kg body weight for an additional 8 weeks.Gut microbiota diversity and composition were assessed by bacterial 16S rRNA amplicon sequencing while colonic tissue was subjected to histopathological staining.Pro-inflammatory gene and cytokine expression were quantified by real-time PCR and ELISA methods.Morin treatment significantly enhanced the abundance of beneficial bacterial taxa,especially Akkermansiaceae,and reduced the prevalence of potentially harmful taxa.Histological evaluation showed that morin attenuated high-fat diet induced colonic hyperplasia and preserved colonic integrity.Moreover,morin administration reduced the expression of key pro-inflammatory genes and cytokines such as TNF-α,IL-6,and increased the anti-inflammatory cytokine,IL-10 in the colon tissue.These findings suggest that morin mitigated high-fat diet induced inflammation by modulating gut microbiota composition thereby reducing colonic inflammation,thereby highlighting its potential as a therapeutic candidate for obesity-related metabolic disorders and warranting further clinical investigation.展开更多
基金Venkat Prashanth sincerely acknowledge Council for Scientific and Industrial Research(CSIR),New Delhi,India,for the award of Junior Research Fellowship(File No:31/005(0575)/2019-EMR-I)Madan Kumar Perumal gratefully acknowledge Indian Council of Medical Research(ICMR)New Delhi,India,for the award of the Extramural Adhoc projects(GAP606,File No:5/4/8-10/OBS/MKP/2021-NCD-II,dated March 30,2021)and(GAP637,File.No:October 52,2022/-BIO/BMS,dated March 18,2023).
文摘Obesity is a complex metabolic disorder associated with chronic low-grade inflammation and gut microbiota dysbiosis.This study evaluated the therapeutic effects of morin,a natural flavonoid,in mitigating inflammation and metabolic disturbances induced by a high-fat diet in male C57BL/6 mice.Mice were fed either a control diet or high-fat diet for 16 weeks,and after obesity was induced,a subset of high-fat diet fed mice received morin via oral gavage at a dose of 100 mg/kg body weight for an additional 8 weeks.Gut microbiota diversity and composition were assessed by bacterial 16S rRNA amplicon sequencing while colonic tissue was subjected to histopathological staining.Pro-inflammatory gene and cytokine expression were quantified by real-time PCR and ELISA methods.Morin treatment significantly enhanced the abundance of beneficial bacterial taxa,especially Akkermansiaceae,and reduced the prevalence of potentially harmful taxa.Histological evaluation showed that morin attenuated high-fat diet induced colonic hyperplasia and preserved colonic integrity.Moreover,morin administration reduced the expression of key pro-inflammatory genes and cytokines such as TNF-α,IL-6,and increased the anti-inflammatory cytokine,IL-10 in the colon tissue.These findings suggest that morin mitigated high-fat diet induced inflammation by modulating gut microbiota composition thereby reducing colonic inflammation,thereby highlighting its potential as a therapeutic candidate for obesity-related metabolic disorders and warranting further clinical investigation.
