Objective:To assess the hepatoprotective effct of Ipomoea carnea(I.carrnea)extract against antitubercular drug-induced liver toxicity in experimental animals.Methods:I.carnea extracts(125,250 and 500 mg/kg,p.o.body we...Objective:To assess the hepatoprotective effct of Ipomoea carnea(I.carrnea)extract against antitubercular drug-induced liver toxicity in experimental animals.Methods:I.carnea extracts(125,250 and 500 mg/kg,p.o.body weight)were administered daily for 35 d in experimental animals.Liver toxicity was induced by combination of three antitubercular drugs(isoniazid 7.5 mg/kg,rifampicin 10 mg/kg and pyrazinamide 35 mg/kg)given orally as suspension for 35 d in rats.Treatment groups received I.carnea extracts along with antitubercular drugs.The hepatoprotective activity was assessed using various biochemical parameters like aspartate aminotransferase,alanine aminotransferase,alkaline phosphatise and total bilirubin.Meanwhile,in-vivo antioxidant activities as lipid peroxidation,reduced glutathione,superoxide dismutase and catalase were measured in rat liver homogenate along with ATPase and G-6-Pase.The biochemical observations were supplemented by histopathological examination.Results:Obtained results demonstrated that treatment with I.carnea extracts significantly(P<0.05-P<0.001)and dose-dependently prevented drug induced increase in serum levels of hepatic enzymes.Furthermore,I.carnea extracts significantly(up to P<0.001)reduced the lipid peroxidation in the liver tissue and restored activities of defence antioxidant enzymes,reduced glutathione,superoxide dismutase and catalase towards normal levels.Histopathology of the liver tissue showed that I.carnea extracts attenuated the hepatocellular necrosis,massive fatty changes and led to reduction in inflammatory cells infiltration.Conclusions:The results of this study strongly indicate the protective effect of I.carnea extracts against liver injury,which may be attributed to its hepatoprotective activity,and there by scientifically support its traditional use.展开更多
Objective:To evaluate the anti-inflammatory effect of Nardostachys jatamansi(N.jatamansi)rhizome against acute,subacute and chronic models of inflammation in experimental animals.Methods:N.jatamansi rhizome extract(15...Objective:To evaluate the anti-inflammatory effect of Nardostachys jatamansi(N.jatamansi)rhizome against acute,subacute and chronic models of inflammation in experimental animals.Methods:N.jatamansi rhizome extract(150 and 300 mg/kg,p.o.)and the reference drugs phenylbutazone(100 mg/kg,p.o.)and acetylsalicylic acid(300 mg/kg,p.o.)were evaluated using models for inflammation(autacoids induced hind paw oedema,formaldehyde induced hind paw oedema,carrageenin-induced paw oedema,cotton pellet granuloma and subcutaneous air pouch model).Results:In acute inflammation as produced by carrageenin 29.06%and 55.81%,by histamine 25.0%and 39.28%,by 5-hydroxytryptamine 21.37%and 36.95%and by prostaglandin E2-induced hind paw oedema 31.03%and 44.82%protection was observed.While in subacute anti-inflammatory models using formaldehyde-induced hind paw oedema(after 1.5 h)13.88%and 33.33%and in chronic anti-inflammatory model using cotton pellet granuloma 7.4%and 17.58%protection from inflammation was observed.N.jatamansi rhizome extract also inhibited the inflammatory mediators(nitric oxide by 12.81%and 38.41%,by prostaglandin E212.58%and 47.82%while by TNF-α13.51%and 41.89%)produced in the pouch.Conclusions:The results of this study strongly indicate the protective effect of N.jatamansi rhizome extract against acute,subacute and chronic models of inflammation,which may be attributed to its anti-inflammatory potential.展开更多
基金Supported by Luqman College of Pharmacy,Gulbarga,Karnataka,(Grant No-LCP/GC/09).
文摘Objective:To assess the hepatoprotective effct of Ipomoea carnea(I.carrnea)extract against antitubercular drug-induced liver toxicity in experimental animals.Methods:I.carnea extracts(125,250 and 500 mg/kg,p.o.body weight)were administered daily for 35 d in experimental animals.Liver toxicity was induced by combination of three antitubercular drugs(isoniazid 7.5 mg/kg,rifampicin 10 mg/kg and pyrazinamide 35 mg/kg)given orally as suspension for 35 d in rats.Treatment groups received I.carnea extracts along with antitubercular drugs.The hepatoprotective activity was assessed using various biochemical parameters like aspartate aminotransferase,alanine aminotransferase,alkaline phosphatise and total bilirubin.Meanwhile,in-vivo antioxidant activities as lipid peroxidation,reduced glutathione,superoxide dismutase and catalase were measured in rat liver homogenate along with ATPase and G-6-Pase.The biochemical observations were supplemented by histopathological examination.Results:Obtained results demonstrated that treatment with I.carnea extracts significantly(P<0.05-P<0.001)and dose-dependently prevented drug induced increase in serum levels of hepatic enzymes.Furthermore,I.carnea extracts significantly(up to P<0.001)reduced the lipid peroxidation in the liver tissue and restored activities of defence antioxidant enzymes,reduced glutathione,superoxide dismutase and catalase towards normal levels.Histopathology of the liver tissue showed that I.carnea extracts attenuated the hepatocellular necrosis,massive fatty changes and led to reduction in inflammatory cells infiltration.Conclusions:The results of this study strongly indicate the protective effect of I.carnea extracts against liver injury,which may be attributed to its hepatoprotective activity,and there by scientifically support its traditional use.
基金Supported by Royal College of Pharmacy and Health Sciences Berhampur(Grant No-RCPHS/PH-09).
文摘Objective:To evaluate the anti-inflammatory effect of Nardostachys jatamansi(N.jatamansi)rhizome against acute,subacute and chronic models of inflammation in experimental animals.Methods:N.jatamansi rhizome extract(150 and 300 mg/kg,p.o.)and the reference drugs phenylbutazone(100 mg/kg,p.o.)and acetylsalicylic acid(300 mg/kg,p.o.)were evaluated using models for inflammation(autacoids induced hind paw oedema,formaldehyde induced hind paw oedema,carrageenin-induced paw oedema,cotton pellet granuloma and subcutaneous air pouch model).Results:In acute inflammation as produced by carrageenin 29.06%and 55.81%,by histamine 25.0%and 39.28%,by 5-hydroxytryptamine 21.37%and 36.95%and by prostaglandin E2-induced hind paw oedema 31.03%and 44.82%protection was observed.While in subacute anti-inflammatory models using formaldehyde-induced hind paw oedema(after 1.5 h)13.88%and 33.33%and in chronic anti-inflammatory model using cotton pellet granuloma 7.4%and 17.58%protection from inflammation was observed.N.jatamansi rhizome extract also inhibited the inflammatory mediators(nitric oxide by 12.81%and 38.41%,by prostaglandin E212.58%and 47.82%while by TNF-α13.51%and 41.89%)produced in the pouch.Conclusions:The results of this study strongly indicate the protective effect of N.jatamansi rhizome extract against acute,subacute and chronic models of inflammation,which may be attributed to its anti-inflammatory potential.
