BACKGROUND Osteoarthritis(OA)is a prevalent joint disorder requiring innovative treatment approaches.AIM To evaluate the use of nanofat,a specialized form of adipose tissue-derived cells,in the treatment of OA,by exam...BACKGROUND Osteoarthritis(OA)is a prevalent joint disorder requiring innovative treatment approaches.AIM To evaluate the use of nanofat,a specialized form of adipose tissue-derived cells,in the treatment of OA,by examining its efficacy,safety profile,mechanisms of action,comparative effectiveness,and long-term outcomes.METHODS A comprehensive review of preclinical studies,clinical trials,and in vitro investigations was conducted.The included studies provided insights into the potential role of nanofat in OA treatment,addressing its efficacy,safety profile,mechanisms of action,comparative effectiveness,and long-term outcomes.RESULTS Clinical studies consistently reported the efficacy of nanofat in providing pain relief and functional improvement in patients with OA.Local adverse events were limited to the injection site,such as localized pain and inflammation,and resolved within a few days to weeks.Systemic adverse events were rare,and no significant long-term complications were observed.Mechanistically,nanofat was found to enhance chondrocyte proliferation,reduce inflammation,and promote angiogenesis,thereby contributing to its therapeutic effects.CONCLUSION Nanofat therapy holds promise as a therapeutic option for managing OA,providing pain relief,functional improvement,and potential tissue regeneration.The safety profile of nanofat treatment appears favorable,but long-term data are still limited.Standardized protocols,larger randomized controlled trials,longer follow-up periods,and cost-effectiveness evaluations are warranted to establish optimal protocols,comparative effectiveness,and long-term outcomes.Despite current limitations,nanofat therapy demonstrates translational potential and should be considered in clinical practice for OA treatment,with careful patient selection and monitoring.展开更多
In regenerative medicine,the isolation of mesenchymal stromal cells(MSCs)from the adipose tissue’s stromal vascular fraction(SVF)is a critical area of study.Our review meticulously examines the isolation process of M...In regenerative medicine,the isolation of mesenchymal stromal cells(MSCs)from the adipose tissue’s stromal vascular fraction(SVF)is a critical area of study.Our review meticulously examines the isolation process of MSCs,starting with the extraction of adipose tissue.The choice of liposuction technique,anatomical site,and immediate processing are essential to maintain cell functionality.We delve into the intricacies of enzymatic digestion,emphasizing the fine-tuning of enzyme concentrations to maximize cell yield while preventing harm.The review then outlines the filtration and centrifugation techniques necessary for isolating a purified SVF,alongside cell viability assessments like flow cytometry,which are vital for confirming the efficacy of the isolated MSCs.We discuss the advantages and drawbacks of using autologous vs allogeneic SVF sources,touching upon immunocompatibility and logistical considerations,as well as the variability inherent in donor-derived cells.Anesthesia choices,the selection between hypo-dermic needles vs liposuction cannulas,and the role of adipose tissue lysers in achieving cellular dissociation are evaluated for their impact on SVF isolation.Centrifugation protocols are also analyzed for their part in ensuring the integrity of the SVF.The necessity for standardized MSC isolation protocols is highlighted,promoting reproducibility and successful clinical application.We encourage ongoing research to deepen the understanding of MSC biology and therapeutic action,aiming to further the field of regenerative medicine.The review concludes with a call for rigorous research,interdisciplinary collaboration,and strict adherence to ethical and regulatory standards to safeguard patient safety and optimize treatment outcomes with MSCs.展开更多
文摘BACKGROUND Osteoarthritis(OA)is a prevalent joint disorder requiring innovative treatment approaches.AIM To evaluate the use of nanofat,a specialized form of adipose tissue-derived cells,in the treatment of OA,by examining its efficacy,safety profile,mechanisms of action,comparative effectiveness,and long-term outcomes.METHODS A comprehensive review of preclinical studies,clinical trials,and in vitro investigations was conducted.The included studies provided insights into the potential role of nanofat in OA treatment,addressing its efficacy,safety profile,mechanisms of action,comparative effectiveness,and long-term outcomes.RESULTS Clinical studies consistently reported the efficacy of nanofat in providing pain relief and functional improvement in patients with OA.Local adverse events were limited to the injection site,such as localized pain and inflammation,and resolved within a few days to weeks.Systemic adverse events were rare,and no significant long-term complications were observed.Mechanistically,nanofat was found to enhance chondrocyte proliferation,reduce inflammation,and promote angiogenesis,thereby contributing to its therapeutic effects.CONCLUSION Nanofat therapy holds promise as a therapeutic option for managing OA,providing pain relief,functional improvement,and potential tissue regeneration.The safety profile of nanofat treatment appears favorable,but long-term data are still limited.Standardized protocols,larger randomized controlled trials,longer follow-up periods,and cost-effectiveness evaluations are warranted to establish optimal protocols,comparative effectiveness,and long-term outcomes.Despite current limitations,nanofat therapy demonstrates translational potential and should be considered in clinical practice for OA treatment,with careful patient selection and monitoring.
文摘In regenerative medicine,the isolation of mesenchymal stromal cells(MSCs)from the adipose tissue’s stromal vascular fraction(SVF)is a critical area of study.Our review meticulously examines the isolation process of MSCs,starting with the extraction of adipose tissue.The choice of liposuction technique,anatomical site,and immediate processing are essential to maintain cell functionality.We delve into the intricacies of enzymatic digestion,emphasizing the fine-tuning of enzyme concentrations to maximize cell yield while preventing harm.The review then outlines the filtration and centrifugation techniques necessary for isolating a purified SVF,alongside cell viability assessments like flow cytometry,which are vital for confirming the efficacy of the isolated MSCs.We discuss the advantages and drawbacks of using autologous vs allogeneic SVF sources,touching upon immunocompatibility and logistical considerations,as well as the variability inherent in donor-derived cells.Anesthesia choices,the selection between hypo-dermic needles vs liposuction cannulas,and the role of adipose tissue lysers in achieving cellular dissociation are evaluated for their impact on SVF isolation.Centrifugation protocols are also analyzed for their part in ensuring the integrity of the SVF.The necessity for standardized MSC isolation protocols is highlighted,promoting reproducibility and successful clinical application.We encourage ongoing research to deepen the understanding of MSC biology and therapeutic action,aiming to further the field of regenerative medicine.The review concludes with a call for rigorous research,interdisciplinary collaboration,and strict adherence to ethical and regulatory standards to safeguard patient safety and optimize treatment outcomes with MSCs.
