Objective:To determine the occurrence of class A and class Cβ-Iactamase genes and their cooccurrence in Indian Enterobacteriaceae.Methods:52 third generation cephalosporin resistant isolates were phenotypically detec...Objective:To determine the occurrence of class A and class Cβ-Iactamase genes and their cooccurrence in Indian Enterobacteriaceae.Methods:52 third generation cephalosporin resistant isolates were phenotypically detected by combination disk method and screened by PCR to identify class A and class C typeβ-lactamase genes.Results:Of the 52 isolates,94.2%（49） were found harboring any of the bla<sub>?</sub>,bla<sub>CTX-M</sub>,bla<sub>SHV</sub> and bla<sub>TEM</sub> were present in 82.6%（43/52）, 59.6%（31/52）,and 42.3%（22/52） isolates,respectively.Of the 49 ESBL positive isolates 57.1% （28/49） showed co-occurrence of bla<sub>ampC</sub>with bla<sub>?</sub>.On the contrary,the collection from 2009 showed their co-occurrence in 81.4%isolates.Conclusions:The comparative study shows a downward trend for co-existence of bla<sub>?</sub> with bla<sub>ampC</sub> from 2009 to 2010.Further large scale studies are needed to address the co-occurrence of class A and class Cβ-lactamases in India and the resistance trend occurring over a period of time.展开更多
A workshop on‘Climate Change and its Impact on Water Resources’was organised by Centre for Earth and Space Science,Uni-versity of Hyderabad,Hyderabad,India during 8th to 10th November 2010.The impact of climate chan...A workshop on‘Climate Change and its Impact on Water Resources’was organised by Centre for Earth and Space Science,Uni-versity of Hyderabad,Hyderabad,India during 8th to 10th November 2010.The impact of climate change on freshwater systems and water resources needs to be quantified and modeled for better management.Projected changes in the frequency and severity of extreme climate events have significant consequence on agriculture and food production.A novel idea has been proposed in the model presented in this article,which calls for an integrated approach from various disciplines to create an awareness on climate change effects on agriculture and environment.展开更多
基金Partially funded by Department of Science & Technology,Ministry of Science & Technology,Govt.of India under Scientist Project SR/FT/L-111/2006
文摘Objective:To determine the occurrence of class A and class Cβ-Iactamase genes and their cooccurrence in Indian Enterobacteriaceae.Methods:52 third generation cephalosporin resistant isolates were phenotypically detected by combination disk method and screened by PCR to identify class A and class C typeβ-lactamase genes.Results:Of the 52 isolates,94.2%（49） were found harboring any of the bla<sub>?</sub>,bla<sub>CTX-M</sub>,bla<sub>SHV</sub> and bla<sub>TEM</sub> were present in 82.6%（43/52）, 59.6%（31/52）,and 42.3%（22/52） isolates,respectively.Of the 49 ESBL positive isolates 57.1% （28/49） showed co-occurrence of bla<sub>ampC</sub>with bla<sub>?</sub>.On the contrary,the collection from 2009 showed their co-occurrence in 81.4%isolates.Conclusions:The comparative study shows a downward trend for co-existence of bla<sub>?</sub> with bla<sub>ampC</sub> from 2009 to 2010.Further large scale studies are needed to address the co-occurrence of class A and class Cβ-lactamases in India and the resistance trend occurring over a period of time.
文摘A workshop on‘Climate Change and its Impact on Water Resources’was organised by Centre for Earth and Space Science,Uni-versity of Hyderabad,Hyderabad,India during 8th to 10th November 2010.The impact of climate change on freshwater systems and water resources needs to be quantified and modeled for better management.Projected changes in the frequency and severity of extreme climate events have significant consequence on agriculture and food production.A novel idea has been proposed in the model presented in this article,which calls for an integrated approach from various disciplines to create an awareness on climate change effects on agriculture and environment.
