This study aimed to develop bioactive films based on curdlan(CD)and sodium alginate(SA)to enhance the shelf life of the Volvariella volvacea mushroom.A stable nanoemulsion of droplet size of 150.1±5 nm was first ...This study aimed to develop bioactive films based on curdlan(CD)and sodium alginate(SA)to enhance the shelf life of the Volvariella volvacea mushroom.A stable nanoemulsion of droplet size of 150.1±5 nm was first formulated using 5%soy protein(Sp)and 10%lavender essential oil(LEO),achieved by ultrasound treatment for 10 min,resulting in small droplet sizes.In parallel,a CD-SA film was prepared by mixing them in a 1:1 ratio.The SpLEO nanoemulsion was then ex situ added to the CD-SA film at varying concentrations(4%,6%,8%,and 10%).The resulting bioactive film exhibited desirable properties,including water vapor permeability(1.15±0.05×10^(-10)g m^(-1)Pa^(-1)s^(-1)),swelling rate(547%),and tensile strength(10.1±1.3 MPa).Docking and structural analyses suggested that ultrasound treatment enhanced the intermolecular hydrogen bonding,creating a uniform and regular surface and reduced crystallinity.The CD-SA film with ultrasound-treated 8%SpLEO nanoemulsion[i.e.,(CD-SA/SpLEO-8)US]showed antibacterial activity against Escherichia coli and Staphylococcus aureus and extended the freshness of straw mushrooms prevention of spoilage during storage.Additionally,the film reduced respiration rate(975 mg CO_(2)/kg/h at 96 h)and ethylene production(160 ng/kg/s by 96 h)while effectively inhibiting straw mushroom autolysis.This led to a reduction in browning(2.91),total soluble solids(7.5%),weight loss(13.9%),and polyphenol oxidase activity(0.08ΔOD_(420)/min⋅g),thereby extending the shelf life of the mushrooms to 96 h.These findings highlight the potential of incorporating different molecules using ultrasound treatment to develop innovative edible films for food preservation.展开更多
Grifola frondosa is a highly valuable edible and medicinal fungus with significant health benefits.In traditional Chinese medicine,has been extensively utilized for its therapeutic properties,playing a vital role in t...Grifola frondosa is a highly valuable edible and medicinal fungus with significant health benefits.In traditional Chinese medicine,has been extensively utilized for its therapeutic properties,playing a vital role in treating various diseases related to immune modulation and inflammation.Polysaccharides are the main active com-pound in G.frondosa,and their biological activities are closely related to their composition.The Grifola frondosa polysaccharides(GFPs)interact with key immune receptors such as Toll-like receptors and Dectin-1,which activate macrophages,natural killer cells,and dendritic cells.This interaction enhances the immune system’s capacity to mount effective responses against infections and malignancies.These polysaccharides control glucose metabolism and regulate insulin sensitivity,indicating their potential for managing and preventing metabolic disorders like diabetes.Although the preclinical studies are promising,further research is essential to standardize extraction techniques,conform clinical efficacy,and clarify the mechanism of health benefits.This review provides a comprehensive overview of recent advances in the production,properties,structural characteristics,and biological activities of GFPs.It highlights their therapeutic potential and promotes further research to support their development and application in functional foods,nutraceuticals,and pharmaceutical formulation.展开更多
基金supported by funding from the Agriculture Research System of Shanghai(Grant No.202209)the Agricultural Field Project of Shanghai"Action Plan for Scientific and Technological Innovation"(23N31900400)+1 种基金the Metasequoia Teacher Research Start-up Fund(163105998)the Researchers Supporting Project number(RSP2024R98),King Saud University,Riyadh,Saudi Arabia,for financial support.
文摘This study aimed to develop bioactive films based on curdlan(CD)and sodium alginate(SA)to enhance the shelf life of the Volvariella volvacea mushroom.A stable nanoemulsion of droplet size of 150.1±5 nm was first formulated using 5%soy protein(Sp)and 10%lavender essential oil(LEO),achieved by ultrasound treatment for 10 min,resulting in small droplet sizes.In parallel,a CD-SA film was prepared by mixing them in a 1:1 ratio.The SpLEO nanoemulsion was then ex situ added to the CD-SA film at varying concentrations(4%,6%,8%,and 10%).The resulting bioactive film exhibited desirable properties,including water vapor permeability(1.15±0.05×10^(-10)g m^(-1)Pa^(-1)s^(-1)),swelling rate(547%),and tensile strength(10.1±1.3 MPa).Docking and structural analyses suggested that ultrasound treatment enhanced the intermolecular hydrogen bonding,creating a uniform and regular surface and reduced crystallinity.The CD-SA film with ultrasound-treated 8%SpLEO nanoemulsion[i.e.,(CD-SA/SpLEO-8)US]showed antibacterial activity against Escherichia coli and Staphylococcus aureus and extended the freshness of straw mushrooms prevention of spoilage during storage.Additionally,the film reduced respiration rate(975 mg CO_(2)/kg/h at 96 h)and ethylene production(160 ng/kg/s by 96 h)while effectively inhibiting straw mushroom autolysis.This led to a reduction in browning(2.91),total soluble solids(7.5%),weight loss(13.9%),and polyphenol oxidase activity(0.08ΔOD_(420)/min⋅g),thereby extending the shelf life of the mushrooms to 96 h.These findings highlight the potential of incorporating different molecules using ultrasound treatment to develop innovative edible films for food preservation.
基金supported by funding from Natural Science Founda-tion of China(32172187,31771961)Chinese Postdoctoral Science Foundation(2023M731377,2023M661761)Science&Technology Platform Construction Program of Jiangxi Province.
文摘Grifola frondosa is a highly valuable edible and medicinal fungus with significant health benefits.In traditional Chinese medicine,has been extensively utilized for its therapeutic properties,playing a vital role in treating various diseases related to immune modulation and inflammation.Polysaccharides are the main active com-pound in G.frondosa,and their biological activities are closely related to their composition.The Grifola frondosa polysaccharides(GFPs)interact with key immune receptors such as Toll-like receptors and Dectin-1,which activate macrophages,natural killer cells,and dendritic cells.This interaction enhances the immune system’s capacity to mount effective responses against infections and malignancies.These polysaccharides control glucose metabolism and regulate insulin sensitivity,indicating their potential for managing and preventing metabolic disorders like diabetes.Although the preclinical studies are promising,further research is essential to standardize extraction techniques,conform clinical efficacy,and clarify the mechanism of health benefits.This review provides a comprehensive overview of recent advances in the production,properties,structural characteristics,and biological activities of GFPs.It highlights their therapeutic potential and promotes further research to support their development and application in functional foods,nutraceuticals,and pharmaceutical formulation.
