A critical challenge for global food security and sustainable agriculture is enhancing crop yields while reducing chemical N inputs.Improving N use efficiency in crops is essential for increasing agricultural producti...A critical challenge for global food security and sustainable agriculture is enhancing crop yields while reducing chemical N inputs.Improving N use efficiency in crops is essential for increasing agricultural productivity.The aim of this study was to evaluate the impacts of intercropping maize with leguminous green manure on grain yield and N utilization under reduced N-fertilization conditions.A field experiment with a split-plot design was conducted in northwestern China from 2018 to 2021.The main plots consisted of two cropping systems:maize-common vetch intercropping(IM)and sole maize(SM).The subplots had three N levels:zero N application(N0,0 kg ha^(-1)),a 25%reduction from the traditional chemical N supply(N1,270 kg ha^(-1)),and the traditional chemical N supply(N2,360 kg ha^(-1)).The results showed that the negative effects of N reduction on maize grain yield and N uptake were compensated by intercropping leguminous green manure,and the improvements increased with cultivation years.The integrated system involving maize-leguminous green manure intercropping and a reduced N supply enhanced N translocation from maize vegetative organs to grains and increased the nitrate reductase and glutamine synthetase activities in maize leaves.The supercompensatory effect in maize leaves increased year by year,reaching values of 16.1,21.3,and 25.5%in 2019,2020,and 2021,respectively.These findings suggest that intercropping maize with leguminous green manure under reduced chemical N input can enhance N assimilation and uptake in maize.By using this strategy,chemical fertilizer is effectively replaced by leguminous green manure,thereby improving N use efficiency and maintaining stable yields in the maize-based intercropping system.展开更多
Additives are widely employed to regulate the morphology,size,and agglomeration degree of crystalline materials during crystallization to enhance their functional,physical,and powder properties.However,the existing me...Additives are widely employed to regulate the morphology,size,and agglomeration degree of crystalline materials during crystallization to enhance their functional,physical,and powder properties.However,the existing methods for screening and validating target additives require a large quantity of materials and involve tedious molecular simulation/crystallization experiments,making them time-consuming,resource-intensive,and reliant on the operator’s experience level.To overcome these challenges,we proposed a computer vision-assisted high-throughput additive screening system(CV-HTPASS)which comprises a high-throughput additive screening device,in situ imaging equipment,and an artificial intelligence(AI)-assisted image-analysis algorithm.Using the CV-HTPASS,we performed high-throughput screening experiments on additives to regulate the succinic acid crystal properties,generating thousands of crystal images with diverse crystal morphologies.To extract valuable crystal information from the massive data and improve the analysis accuracy and efficiency,the AI-based image-analysis algorithm was implemented innovatively for the segmentation,classification,and data mining of crystals with four morphologies to further screen the target additive.Subsequently,scale-up crystallization experiments conducted under optimized conditions demonstrated that succinic acid products exhibited a preferred cubic morphology,reduced agglomeration degree,narrowed crystal size distribution,and improved powder properties.The proposed CV-HTPASS offers a highly efficient approach for scale-up experiments.Further,it provides a platform for the screening of additives and the optimization of the powder properties of crystal products in industrial-scale crystallization processes.展开更多
This paper aims at summarized the research progress of soil microbes,in amount of soil microbes including bacteria,fungi and actinomycetes,soil microbial biomass,including microbial biomass carbon,microbial biomass ni...This paper aims at summarized the research progress of soil microbes,in amount of soil microbes including bacteria,fungi and actinomycetes,soil microbial biomass,including microbial biomass carbon,microbial biomass nitrogen and microbial biomass phosphorus,function of microbial and screening and application of beneficial microorganisms etc.,and future research are discussed combined with our project team for many years of work.展开更多
Background:Plant phyllosphere microbes are important for the host plant's protection.Plant growth-promoting rhizobacteria(PGPR)and Trichoderma are common biocontrol agents(BCAs)for disease management.Pathogens and...Background:Plant phyllosphere microbes are important for the host plant's protection.Plant growth-promoting rhizobacteria(PGPR)and Trichoderma are common biocontrol agents(BCAs)for disease management.Pathogens and BCAs can change the rhizosphere microbial composition;however,the effect of PGPR or Trichoderma on plant phyllosphere microbes,particularly for mesocosms involving the interaction between pathogens and BCAs,is not well known.Methods:High-throughput sequencing was used to identify the phyllosphere bacterial community of common vetch interacting with Colletotrichum spinaciae,two PGPRs(Bacillus subtilis and Bacillus licheniformis),and Trichoderma longibrachiatum.We evaluated anthracnose severity,phyllosphere bacteria diversity and composition,and the relationship between the activities of plant defense enzymes and hormonal molecules in plants treated with individual and combined inoculations of PGPRs,Trichoderma,and C.spinaciae.Results:PGPR or Trichoderma alone reduced disease severity.Trichoderma reduced the salicylic acid content,PGPR increased the catalase activity in plants,and co-inoculation of PGPR and Trichoderma decreased the salicylic acid content.Inoculation of PGPR and Trichoderma individually or in combination changed the disease-associated phyllosphere bacteria,and this effect was related to plant defense enzymes and hormonal molecules.Conclusions:We suggest that the plant defense response induced by PGPR and Trichoderma results in the enrichment of a fraction of favorable chloroplastic bacteria,which facilitates plant defense against diseases.展开更多
基金supported by the‘Double First-Class’Key Scientific Research Project of Education Department in Gansu Province,China(GSSYLXM-02)the National Natural Science Foundation of China(U21A20218 and 32160765)+3 种基金the earmarked fund for China Agriculture Research System(CARS-22-G-12)the Science and Technology Project of Gansu Province,China(20JR5RA037 and 21JR7RA836)the Postdoctoral Research Start-up Foundation of Gansu Province,China(03824034)the Postdoctoral Research Start-up Foundation of Gansu Agricultural University,China(202403)。
文摘A critical challenge for global food security and sustainable agriculture is enhancing crop yields while reducing chemical N inputs.Improving N use efficiency in crops is essential for increasing agricultural productivity.The aim of this study was to evaluate the impacts of intercropping maize with leguminous green manure on grain yield and N utilization under reduced N-fertilization conditions.A field experiment with a split-plot design was conducted in northwestern China from 2018 to 2021.The main plots consisted of two cropping systems:maize-common vetch intercropping(IM)and sole maize(SM).The subplots had three N levels:zero N application(N0,0 kg ha^(-1)),a 25%reduction from the traditional chemical N supply(N1,270 kg ha^(-1)),and the traditional chemical N supply(N2,360 kg ha^(-1)).The results showed that the negative effects of N reduction on maize grain yield and N uptake were compensated by intercropping leguminous green manure,and the improvements increased with cultivation years.The integrated system involving maize-leguminous green manure intercropping and a reduced N supply enhanced N translocation from maize vegetative organs to grains and increased the nitrate reductase and glutamine synthetase activities in maize leaves.The supercompensatory effect in maize leaves increased year by year,reaching values of 16.1,21.3,and 25.5%in 2019,2020,and 2021,respectively.These findings suggest that intercropping maize with leguminous green manure under reduced chemical N input can enhance N assimilation and uptake in maize.By using this strategy,chemical fertilizer is effectively replaced by leguminous green manure,thereby improving N use efficiency and maintaining stable yields in the maize-based intercropping system.
基金supported by the Shandong Provincial Key Research and Development Program(Major Key Technology Project)(2021CXGC010514)the National Natural Science Foundation of China(22008173).
文摘Additives are widely employed to regulate the morphology,size,and agglomeration degree of crystalline materials during crystallization to enhance their functional,physical,and powder properties.However,the existing methods for screening and validating target additives require a large quantity of materials and involve tedious molecular simulation/crystallization experiments,making them time-consuming,resource-intensive,and reliant on the operator’s experience level.To overcome these challenges,we proposed a computer vision-assisted high-throughput additive screening system(CV-HTPASS)which comprises a high-throughput additive screening device,in situ imaging equipment,and an artificial intelligence(AI)-assisted image-analysis algorithm.Using the CV-HTPASS,we performed high-throughput screening experiments on additives to regulate the succinic acid crystal properties,generating thousands of crystal images with diverse crystal morphologies.To extract valuable crystal information from the massive data and improve the analysis accuracy and efficiency,the AI-based image-analysis algorithm was implemented innovatively for the segmentation,classification,and data mining of crystals with four morphologies to further screen the target additive.Subsequently,scale-up crystallization experiments conducted under optimized conditions demonstrated that succinic acid products exhibited a preferred cubic morphology,reduced agglomeration degree,narrowed crystal size distribution,and improved powder properties.The proposed CV-HTPASS offers a highly efficient approach for scale-up experiments.Further,it provides a platform for the screening of additives and the optimization of the powder properties of crystal products in industrial-scale crystallization processes.
基金Supported by Scientific and Technological Innovation Project of Gansu Agriculture and Animal Husbandry Bureaus(GNXC-2012-45)National Natural Science Foundation of China(41161049)
文摘This paper aims at summarized the research progress of soil microbes,in amount of soil microbes including bacteria,fungi and actinomycetes,soil microbial biomass,including microbial biomass carbon,microbial biomass nitrogen and microbial biomass phosphorus,function of microbial and screening and application of beneficial microorganisms etc.,and future research are discussed combined with our project team for many years of work.
基金China Modern Agriculture Research System,Grant/Award Number:CARS-22 Green Manure。
文摘Background:Plant phyllosphere microbes are important for the host plant's protection.Plant growth-promoting rhizobacteria(PGPR)and Trichoderma are common biocontrol agents(BCAs)for disease management.Pathogens and BCAs can change the rhizosphere microbial composition;however,the effect of PGPR or Trichoderma on plant phyllosphere microbes,particularly for mesocosms involving the interaction between pathogens and BCAs,is not well known.Methods:High-throughput sequencing was used to identify the phyllosphere bacterial community of common vetch interacting with Colletotrichum spinaciae,two PGPRs(Bacillus subtilis and Bacillus licheniformis),and Trichoderma longibrachiatum.We evaluated anthracnose severity,phyllosphere bacteria diversity and composition,and the relationship between the activities of plant defense enzymes and hormonal molecules in plants treated with individual and combined inoculations of PGPRs,Trichoderma,and C.spinaciae.Results:PGPR or Trichoderma alone reduced disease severity.Trichoderma reduced the salicylic acid content,PGPR increased the catalase activity in plants,and co-inoculation of PGPR and Trichoderma decreased the salicylic acid content.Inoculation of PGPR and Trichoderma individually or in combination changed the disease-associated phyllosphere bacteria,and this effect was related to plant defense enzymes and hormonal molecules.Conclusions:We suggest that the plant defense response induced by PGPR and Trichoderma results in the enrichment of a fraction of favorable chloroplastic bacteria,which facilitates plant defense against diseases.
