The multilayer of polyethylenimine (PEI) and carrageenan (k, i, l) formed by layer-by-layer assembly was investigated for its antibacterial activity against Enterobacter cloaceae, Staphylococcus aureus and Enterococcu...The multilayer of polyethylenimine (PEI) and carrageenan (k, i, l) formed by layer-by-layer assembly was investigated for its antibacterial activity against Enterobacter cloaceae, Staphylococcus aureus and Enterococcus faecalis 29505 for potential use as coating on biomaterial surface. All the multilayers exhibited growth inhibition. PEI/Iota carrageenan multilayer was effective in inhibiting the growth of the E. cloaceae, S. aureus and E. faecalis while PEI/Lambda carrageenan was effective in inhibiting the growth of E. cloaceae. Results of the paper strip test for combined action of carrageenan and PEI showed synergism with regards to bacterial growth inhibition. The multilayers had also contact-killing effect with the test organisms. The multilayer was also characterized by atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and biomolecular interaction analysis.展开更多
Mild hydrochloric acid hydrolysis of i-carrageenan from Eucheuma spinosum yielded two oligosaccharides of sulfated tetrasaccharide structure. These were characterized by Fourier Transform Infrared Spectroscopy (FT-IR)...Mild hydrochloric acid hydrolysis of i-carrageenan from Eucheuma spinosum yielded two oligosaccharides of sulfated tetrasaccharide structure. These were characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Nuclear Magnetic Resonance (NMR) and Electrospray Ionization Mass Spectrometry (ESIMS). Both oligosaccharides have structure of b-D-galactopyranose(Galp)4S-(1→4)-α-D-AnGalp2S-(1→3)-b-D-galactopyranose Galp)4S-(1→4)-α-D-AnGalp2S-(1→3). Application of the resulting oligosaccharides on protein delivery system in terms of encapsulation efficiency was performed.展开更多
文摘The multilayer of polyethylenimine (PEI) and carrageenan (k, i, l) formed by layer-by-layer assembly was investigated for its antibacterial activity against Enterobacter cloaceae, Staphylococcus aureus and Enterococcus faecalis 29505 for potential use as coating on biomaterial surface. All the multilayers exhibited growth inhibition. PEI/Iota carrageenan multilayer was effective in inhibiting the growth of the E. cloaceae, S. aureus and E. faecalis while PEI/Lambda carrageenan was effective in inhibiting the growth of E. cloaceae. Results of the paper strip test for combined action of carrageenan and PEI showed synergism with regards to bacterial growth inhibition. The multilayers had also contact-killing effect with the test organisms. The multilayer was also characterized by atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and biomolecular interaction analysis.
文摘Mild hydrochloric acid hydrolysis of i-carrageenan from Eucheuma spinosum yielded two oligosaccharides of sulfated tetrasaccharide structure. These were characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Nuclear Magnetic Resonance (NMR) and Electrospray Ionization Mass Spectrometry (ESIMS). Both oligosaccharides have structure of b-D-galactopyranose(Galp)4S-(1→4)-α-D-AnGalp2S-(1→3)-b-D-galactopyranose Galp)4S-(1→4)-α-D-AnGalp2S-(1→3). Application of the resulting oligosaccharides on protein delivery system in terms of encapsulation efficiency was performed.
