The impregnation method for preparing catalysts often faces challenges such as prolonged preparation times and poor dispersion of active components due to the limited mobility of the impregnation liquid.The rotating p...The impregnation method for preparing catalysts often faces challenges such as prolonged preparation times and poor dispersion of active components due to the limited mobility of the impregnation liquid.The rotating packed bed(RPB)can break the precursor solution into fine droplets,enabling dynamic impregnation of active components onto the surface of activated carbon.This approach facilitates the uniform distribution of active components on the carrier and enhances the stability and performance of the catalyst.In this study,activated carbon catalysts were prepared using high-gravity technology.It was found that the preparation time for Co-MnO_(x)/GAC using the RPB method was reduced by 98%,the catalytic activity increased by 6.62%,and the loadings of active components increased by 13%and 17%,the catalytic activity remained stable after five cycles,with a significantly lower rate of metal dissolution.A suite of complementary analytical techniques demonstrates that Co-MnO_(x)/GAC(RPB)has higher homogeneity and dispersion.X-ray photoelectron spectroscopy(XPS)results indicate that Co(II)and Mn(IV)/Mn(III)are the primary active sites during the catalytic decomposition of ozone,elucidating the mechanism of synergistic catalytic ozonation by dual-active components.Finally,electron paramagnetic resonance(EPR)confirmed that hydroxyl radicals($OH)were the predominant reactive species in the reaction.展开更多
Graphene quantum dots(GQDs)are a class of promising carbon-based nanomaterials that have attracted considerable interest from researchers due to their excellent physical,chemical,and biological properties.However,the ...Graphene quantum dots(GQDs)are a class of promising carbon-based nanomaterials that have attracted considerable interest from researchers due to their excellent physical,chemical,and biological properties.However,the high cost,toxicity,and laborious preparation process of GQDs also limit their widespread use.To address this issue,the actual research directions consist in replacing traditional non-renewable feedstocks via screening cheap,easily available,and renewable biomass materials based on the concept of resource conservation and environmental friendliness.Herein,the state-of-the-art technologies in the green preparation of GQDs using biomass as carbon source are reported.Initially,the green synthesis strategies as well as the structural,optical,and biosafety properties of GQDs are discussed in detail.Subsequently,the most representative applications of GQDs in energy and environmental remediation fields are summarized.Finally,the current challenges and future potential of the GQDs are presented.展开更多
Lung cancer has one of the highest rates of incidence and mortality worldwide,mak-ing research on its mechanisms and treatments crucial.Animal models are essential in lung cancer research as they accurately replicate ...Lung cancer has one of the highest rates of incidence and mortality worldwide,mak-ing research on its mechanisms and treatments crucial.Animal models are essential in lung cancer research as they accurately replicate the biological characteristics and treatment outcomes seen in human diseases.Currently,various lung cancer models have been established,including chemical induction models,orthotopic transplan-tation models,ectopic transplantation models,metastasis models,and gene editing mouse models.Additionally,lung cancer grafts can be categorized into two types:tissue-based and cell-based grafts.This paper summarizes the phenotypes,advan-tages,and disadvantages of various induction methods based on their modeling tech-niques.The goal is to enhance the simulation of clinical lung cancer characteristics and to establish a solid foundation for future clinical research.展开更多
In high-frequency electrical energy systems,polyimide(PI)composite insulation materials need to possess a low dielectric constant,sufficient thermal conductivity,and robust interfacial adhesion to ensure reliable perf...In high-frequency electrical energy systems,polyimide(PI)composite insulation materials need to possess a low dielectric constant,sufficient thermal conductivity,and robust interfacial adhesion to ensure reliable performance under elevated temperatures and pressures.These aspects are crucial for preventing local overheating and electrical breakdown,thereby ensuring reliable equipment operation.Traditional PI insulation materials often exhibit high dielectric constants and pronounced dielectric losses,compromising their insulation efficiency.In this study,molecular dynamics simulations were employed to incorporate polyhedral oligomeric silsesquioxanes(POSS)into PI through physical blending and chemical bonding to enhance dielectric properties.Key parameters of the PI/POSS composite system,including dielectric constant,thermal conductivity,glass transition temperature,Young’s modulus,Poisson’s ratio,and interfacial adhesion energy,were systematically evaluated for both doping methods.The degradation behavior of the PI composites under high-temperature and electric field conditions was also simulated to elucidate degradation pathways and product distributions,providing insights for designing low-dielectric insulation materials.Doping with POSS significantly reduces the dielectric constant of PI,thereby improving insulation performance,thermal stability,mechanical strength,and interfacial adhesion.At an optimal POSS doping ratio,the thermal conductivity of PI is enhanced.Compared with the physical blending system,the chemical bonding system yields more substantial improvements across all evaluated properties.Under high-temperature and strong electric field conditions,POSS doping enhances interfacial adhesion and thermal stability,effectively suppressing the cleavage of key chemical bonds,reducingCOemissions,and increasing the formation of oxygen-containing intermediates and water molecules,which contributes to improved environmental sustainability.展开更多
Aeromonas veronii is considered an emerging food-borne pathogen associated with a significant threat to public health,distributed in various aquatic environments and products.Hanks-type serine/threonine kinases(STKs)p...Aeromonas veronii is considered an emerging food-borne pathogen associated with a significant threat to public health,distributed in various aquatic environments and products.Hanks-type serine/threonine kinases(STKs)play a critical role in the pathogenesis of pathogens.However,the function of A.veronii STKs is currently unclear.By constructing a markerless prk A in-frame deletion strain,Δprk A,we found that i)the colonies of theΔprk A strain were larger after 1 h of high temperature at 50℃compared with the wild-type strain TH0426 and the complementary strain C-prk A,and the number of viable bacteria of theΔprk A strain increased significantly;ii)theΔprk A strain significantly enhanced the adhesion ability to epithelioma papulosum cyprini(EPC)cells;iii)theΔprk A strain was significantly more virulent than the TH0426 strain,at both the cellular and animal levels;and iv)RNA-seq results showed a total of 984 differentially expressed genes(DEGs)between theΔprk A strain and the TH0426 strain,which were enriched in 70 Kyoto Encyclopedia of Genes and Genomes(KEGG)metabolic pathways,mainly involved in bacterial ribosomes,flagellar assembly,type Ⅱ secretion system(T2SS),and lipopolysaccharide metabolic pathways.Taken together,the findings of this study indicate that the Hanks-type STK Prk A negatively regulates several biological processes,such as the temperature tolerance and virulence of A.veronii.The results of this study provide an important reference for further elucidation of the pathogenesis of A.veronii.展开更多
Zebrafish larvae are useful for identifying chemicals against lateral line(LL)hair cell(HC)damage and this type of chemical screen mainly focuses on searching for protectors against cell death.To expand the candidate ...Zebrafish larvae are useful for identifying chemicals against lateral line(LL)hair cell(HC)damage and this type of chemical screen mainly focuses on searching for protectors against cell death.To expand the candidate pool of HC protectors,a self-built acoustic escape response(AER)-detecting system was developed to apply both low-frequency near-field sound transmission and AER image acquisition/processing modules.The device quickly confirmed the changed LL HC functions caused by most known ototoxins,protectors,and neural transmission modifiers,or knockdown of LL HC-expressing genes.With ten devices wired in tandem,five‘hit’chemicals were identified from 124 cyclin-dependent kinase inhibitors to partially restore cisplatin-damaged AER in less than a day.AS2863619,ribociclib,and SU9516 among the hits,protected the HCs in the mouse cochlea.Therefore,using free-swimming larval zebrafish,the self-made AER-detecting device can efficiently identify compounds that are protective against HC damage,including cell death and loss-of-function.展开更多
Objective:To present a modified technique for robot-assisted laparoendoscopic single-site retroperitoneal nephroureterectomy(RLESS-RNU)with bladder cuff excision and evaluate its effectiveness as a minimally invasive ...Objective:To present a modified technique for robot-assisted laparoendoscopic single-site retroperitoneal nephroureterectomy(RLESS-RNU)with bladder cuff excision and evaluate its effectiveness as a minimally invasive approach for managing upper tract urothelial carcinoma(UTUC).Methods:Fifteen patients who underwent RLESS-RNU for UTUC between April 2020 and June 2021 were reviewed at our medical institution(The Second Affiliated Hospital of Nanjing Medical University,Nanjing,China),from our prospectively maintained institutional database.The da Vinci^(®)Xi system(Intuitive Surgical,Sunnyvale,CA,USA)was utilized for surgical procedures in all patients.An in-depth analysis was conducted on their baseline demographic characteristics,pathological factors,and perioperative details.The complete surgical process and details are elaborated.Results:The median age of 15 patients was 67 years.The final pathology demonstrated 47%(7/15)patients with pT1 or lower and 47%(7/15)with pT3;one patient could not undergo pathological staging because of preoperative chemotherapy.The perioperative outcomes revealed that the mean operative time was 185(standard deviation[SD]23.05)min.The mean times of the trocar placement for primary and second docking were 15.00(SD 0.85)min and 8.00(SD 0.52)min,respectively.The median estimated blood loss was 55 mL.The mean drainage tube duration and postoperative hospital stay were 7.50 days and 11.00 days,respectively.After a mean follow-up period of 24.20 months,the relapse-free survival rate was 87%.Two patients experienced disease progression:one patient exhibited multifocal evidence of non-muscle invasive bladder cancer,and the other patient developed systemic recurrence.Conclusion:This study demonstrates that the modified technique for RLESS-RNU is safe and satisfactory for UTUC.展开更多
This study investigated the effects of planting duration(1,5,10 and 15 years)on soil properties,bacterial community diversity,and function in the rhizosphere of Zanthoxylum bungeanum.We employed Illumina highthroughpu...This study investigated the effects of planting duration(1,5,10 and 15 years)on soil properties,bacterial community diversity,and function in the rhizosphere of Zanthoxylum bungeanum.We employed Illumina highthroughput sequencing and PICRUSt2 functional prediction to analyze the structure and functional potential of rhizosphere soil bacterial communities.The Mantel test and redundancy analysis were used to identify physicochemical factors influencing bacterial community structure and function.The results indicated significant differences in rhizosphere soil physicochemical properties across planting years:the content of organic matter,alkaline hydrolyzable nitrogen in the soil,as well as the activity of invertase,urease,and alkaline phosphatase initially increased and then decreased,while available potassium,Olsen-phosphorus content,and peroxidase activity continued to increase.However,bacterial alpha diversity(Chao1 and Shannon indices)and the number of amplicon sequence variants increased continuously with planting duration.Principal coordinate analysis and Adonis tests revealed that the planting year significantly influenced the bacterial community structure(p<0.05).The phyla Proteobacteria,Actinobacteria,Acidobacteriota and Chloroflexi collectively constituted 56.7%to 71.2%of the relative abundance,representing the dominant taxa.PICRUSt2 predictions indicated key functional categories(cellular processes,metabolism,genetic information processing,and environmental information processing)each exceeding 10%relative abundance.BugBase analysis revealed a progressive increase in aerobic and oxidative stress-tolerant bacteria and a decrease in anaerobic and potentially pathogenic bacteria.Differential indicator species analysis identified Firmicutes,Planctomycetes,Methylomirabilota and Actinobacteriota as key discriminators for the 1-,5-,10-and 15-year stages,respectively.Organic matter,alkaline phosphatase,soil pH,and available phosphorus were the primary physicochemical drivers of bacterial communities.Notably,soil organic matter significantly influenced both the community structure(p<0.05)and predictedmetabolic functions(p<0.05).In conclusion,prolonged planting duration significantly enhanced rhizosphere microbial diversity and functional gene abundance in Z.bungeanumwhile driving the structural succession of bacterial communities dominated by Proteobacteria,Actinobacteria,Acidobacteriota,and Chloroflexi.This ecological shift,characterized by increased aerobic/oxidative-stress taxa and decreased anaerobic/pathogenic bacteria,was primarily regulated by soil organic matter,a key driver shaping both community structure and metabolic functions,ultimately improving soil microecological health.展开更多
As an active flow control technology and with the advantages of no moving components, the Sweeping jet actuator has become a hotspot in the field of flow control. However, the linear relationship between oscillation f...As an active flow control technology and with the advantages of no moving components, the Sweeping jet actuator has become a hotspot in the field of flow control. However, the linear relationship between oscillation frequency and momentum coefficient in a sweeping jet actuator makes it difficult to determine the dominant factors that affect control effectiveness. Decoupling the oscillation frequency and momentum coefficient, as well as determining the control mechanism, is the focus of studying the sweeping jet actuator. In this study, a novel sweeping jet actuator is designed using synthetic jets instead of feedback channels and applied to the flow separation control of NACA0018 airfoil. This article studies the control effect under three oscillation frequencies of F<sup>+</sup> = f × c/U<sub>∞</sub> = 1, 10, 100 and three momentum coefficients of C<sub>μ</sub> = 0.45%, 0.625%, 0.9%. The numerical results indicate that all three oscillation frequencies have good control effects on flow separation, and the control effect is best when F<sup>+</sup> = 1, with the maximum lift coefficient increasing by approximately 14% compared to the other two cases. And the sweeping jet actuator has a better ability to control flow separation as the momentum coefficient increases. By decoupling the characteristics of the sweeping jet actuator and conducting numerical analysis of the flow control effect, it will promote its better engineering application in the field of flow control. .展开更多
基金supported by the National Natural Science Foundation of China(U23A20676)Key Research&Development Plan of Shanxi Province(202202040201011)+3 种基金Shanxi Scholarship Council of China(2023-128)Small and medium-sized oriented scientific and technological enterprises innovation ability improvement project of Shandong Province(2023TSGC0004)the Graduate Student Innovation Project of Shanxi Province(2023SJ205)Local Funds for Science and Technology Development Guided by the Central Finance(YDZJSX20231A030).
文摘The impregnation method for preparing catalysts often faces challenges such as prolonged preparation times and poor dispersion of active components due to the limited mobility of the impregnation liquid.The rotating packed bed(RPB)can break the precursor solution into fine droplets,enabling dynamic impregnation of active components onto the surface of activated carbon.This approach facilitates the uniform distribution of active components on the carrier and enhances the stability and performance of the catalyst.In this study,activated carbon catalysts were prepared using high-gravity technology.It was found that the preparation time for Co-MnO_(x)/GAC using the RPB method was reduced by 98%,the catalytic activity increased by 6.62%,and the loadings of active components increased by 13%and 17%,the catalytic activity remained stable after five cycles,with a significantly lower rate of metal dissolution.A suite of complementary analytical techniques demonstrates that Co-MnO_(x)/GAC(RPB)has higher homogeneity and dispersion.X-ray photoelectron spectroscopy(XPS)results indicate that Co(II)and Mn(IV)/Mn(III)are the primary active sites during the catalytic decomposition of ozone,elucidating the mechanism of synergistic catalytic ozonation by dual-active components.Finally,electron paramagnetic resonance(EPR)confirmed that hydroxyl radicals($OH)were the predominant reactive species in the reaction.
基金supported by the following funding:National Natural Science Foundation of China(Nos.52070057 and 51961165104)Project of a Thousand Youth Talents(No.AUGA2160100917)Open Project of State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology(No.2019DX09)。
文摘Graphene quantum dots(GQDs)are a class of promising carbon-based nanomaterials that have attracted considerable interest from researchers due to their excellent physical,chemical,and biological properties.However,the high cost,toxicity,and laborious preparation process of GQDs also limit their widespread use.To address this issue,the actual research directions consist in replacing traditional non-renewable feedstocks via screening cheap,easily available,and renewable biomass materials based on the concept of resource conservation and environmental friendliness.Herein,the state-of-the-art technologies in the green preparation of GQDs using biomass as carbon source are reported.Initially,the green synthesis strategies as well as the structural,optical,and biosafety properties of GQDs are discussed in detail.Subsequently,the most representative applications of GQDs in energy and environmental remediation fields are summarized.Finally,the current challenges and future potential of the GQDs are presented.
基金Sichuan Provincial Administration of Traditional Chinese Medicine,Grant/Award Number:2023MS564National Natural Science Foundation of China,Grant/Award Number:82474436。
文摘Lung cancer has one of the highest rates of incidence and mortality worldwide,mak-ing research on its mechanisms and treatments crucial.Animal models are essential in lung cancer research as they accurately replicate the biological characteristics and treatment outcomes seen in human diseases.Currently,various lung cancer models have been established,including chemical induction models,orthotopic transplan-tation models,ectopic transplantation models,metastasis models,and gene editing mouse models.Additionally,lung cancer grafts can be categorized into two types:tissue-based and cell-based grafts.This paper summarizes the phenotypes,advan-tages,and disadvantages of various induction methods based on their modeling tech-niques.The goal is to enhance the simulation of clinical lung cancer characteristics and to establish a solid foundation for future clinical research.
基金financially supported by the Natural Science Foundation of Shandong Province(No.ZR2021ME011)State Key Laboratory of Alternate Electrical Power System with Renewable Energy Sources(No.LAPS20001)。
文摘In high-frequency electrical energy systems,polyimide(PI)composite insulation materials need to possess a low dielectric constant,sufficient thermal conductivity,and robust interfacial adhesion to ensure reliable performance under elevated temperatures and pressures.These aspects are crucial for preventing local overheating and electrical breakdown,thereby ensuring reliable equipment operation.Traditional PI insulation materials often exhibit high dielectric constants and pronounced dielectric losses,compromising their insulation efficiency.In this study,molecular dynamics simulations were employed to incorporate polyhedral oligomeric silsesquioxanes(POSS)into PI through physical blending and chemical bonding to enhance dielectric properties.Key parameters of the PI/POSS composite system,including dielectric constant,thermal conductivity,glass transition temperature,Young’s modulus,Poisson’s ratio,and interfacial adhesion energy,were systematically evaluated for both doping methods.The degradation behavior of the PI composites under high-temperature and electric field conditions was also simulated to elucidate degradation pathways and product distributions,providing insights for designing low-dielectric insulation materials.Doping with POSS significantly reduces the dielectric constant of PI,thereby improving insulation performance,thermal stability,mechanical strength,and interfacial adhesion.At an optimal POSS doping ratio,the thermal conductivity of PI is enhanced.Compared with the physical blending system,the chemical bonding system yields more substantial improvements across all evaluated properties.Under high-temperature and strong electric field conditions,POSS doping enhances interfacial adhesion and thermal stability,effectively suppressing the cleavage of key chemical bonds,reducingCOemissions,and increasing the formation of oxygen-containing intermediates and water molecules,which contributes to improved environmental sustainability.
基金The Shandong Natural Science Foundation Youth Fund Project(ZR2022QC079,ZR2023QD024)provided funding for this work。
文摘Aeromonas veronii is considered an emerging food-borne pathogen associated with a significant threat to public health,distributed in various aquatic environments and products.Hanks-type serine/threonine kinases(STKs)play a critical role in the pathogenesis of pathogens.However,the function of A.veronii STKs is currently unclear.By constructing a markerless prk A in-frame deletion strain,Δprk A,we found that i)the colonies of theΔprk A strain were larger after 1 h of high temperature at 50℃compared with the wild-type strain TH0426 and the complementary strain C-prk A,and the number of viable bacteria of theΔprk A strain increased significantly;ii)theΔprk A strain significantly enhanced the adhesion ability to epithelioma papulosum cyprini(EPC)cells;iii)theΔprk A strain was significantly more virulent than the TH0426 strain,at both the cellular and animal levels;and iv)RNA-seq results showed a total of 984 differentially expressed genes(DEGs)between theΔprk A strain and the TH0426 strain,which were enriched in 70 Kyoto Encyclopedia of Genes and Genomes(KEGG)metabolic pathways,mainly involved in bacterial ribosomes,flagellar assembly,type Ⅱ secretion system(T2SS),and lipopolysaccharide metabolic pathways.Taken together,the findings of this study indicate that the Hanks-type STK Prk A negatively regulates several biological processes,such as the temperature tolerance and virulence of A.veronii.The results of this study provide an important reference for further elucidation of the pathogenesis of A.veronii.
基金supported by grants from Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions(2023SHIBS0002)the National Key Research and Development Program of China(2023YFF1203505)+5 种基金the National Key R&D Program of China(2024YFC2417900)Shenzhen Municipal Science and Technology Innovation Council(202110293000007)Shenzhen Science and Technology Program(KJZD20230923114909019)the National Key R&D Program of China(2018YFA0801006)the National Natural Science Foundation of China(31771618)Guangdong Natural Science Foundation(2022A1515011878).
文摘Zebrafish larvae are useful for identifying chemicals against lateral line(LL)hair cell(HC)damage and this type of chemical screen mainly focuses on searching for protectors against cell death.To expand the candidate pool of HC protectors,a self-built acoustic escape response(AER)-detecting system was developed to apply both low-frequency near-field sound transmission and AER image acquisition/processing modules.The device quickly confirmed the changed LL HC functions caused by most known ototoxins,protectors,and neural transmission modifiers,or knockdown of LL HC-expressing genes.With ten devices wired in tandem,five‘hit’chemicals were identified from 124 cyclin-dependent kinase inhibitors to partially restore cisplatin-damaged AER in less than a day.AS2863619,ribociclib,and SU9516 among the hits,protected the HCs in the mouse cochlea.Therefore,using free-swimming larval zebrafish,the self-made AER-detecting device can efficiently identify compounds that are protective against HC damage,including cell death and loss-of-function.
文摘Objective:To present a modified technique for robot-assisted laparoendoscopic single-site retroperitoneal nephroureterectomy(RLESS-RNU)with bladder cuff excision and evaluate its effectiveness as a minimally invasive approach for managing upper tract urothelial carcinoma(UTUC).Methods:Fifteen patients who underwent RLESS-RNU for UTUC between April 2020 and June 2021 were reviewed at our medical institution(The Second Affiliated Hospital of Nanjing Medical University,Nanjing,China),from our prospectively maintained institutional database.The da Vinci^(®)Xi system(Intuitive Surgical,Sunnyvale,CA,USA)was utilized for surgical procedures in all patients.An in-depth analysis was conducted on their baseline demographic characteristics,pathological factors,and perioperative details.The complete surgical process and details are elaborated.Results:The median age of 15 patients was 67 years.The final pathology demonstrated 47%(7/15)patients with pT1 or lower and 47%(7/15)with pT3;one patient could not undergo pathological staging because of preoperative chemotherapy.The perioperative outcomes revealed that the mean operative time was 185(standard deviation[SD]23.05)min.The mean times of the trocar placement for primary and second docking were 15.00(SD 0.85)min and 8.00(SD 0.52)min,respectively.The median estimated blood loss was 55 mL.The mean drainage tube duration and postoperative hospital stay were 7.50 days and 11.00 days,respectively.After a mean follow-up period of 24.20 months,the relapse-free survival rate was 87%.Two patients experienced disease progression:one patient exhibited multifocal evidence of non-muscle invasive bladder cancer,and the other patient developed systemic recurrence.Conclusion:This study demonstrates that the modified technique for RLESS-RNU is safe and satisfactory for UTUC.
基金supported by Forestry and Grassland Science and Technology Innovation Project(LCKJCX2022001)from Forestry and Grassland Bureau of Gansu Province’s.
文摘This study investigated the effects of planting duration(1,5,10 and 15 years)on soil properties,bacterial community diversity,and function in the rhizosphere of Zanthoxylum bungeanum.We employed Illumina highthroughput sequencing and PICRUSt2 functional prediction to analyze the structure and functional potential of rhizosphere soil bacterial communities.The Mantel test and redundancy analysis were used to identify physicochemical factors influencing bacterial community structure and function.The results indicated significant differences in rhizosphere soil physicochemical properties across planting years:the content of organic matter,alkaline hydrolyzable nitrogen in the soil,as well as the activity of invertase,urease,and alkaline phosphatase initially increased and then decreased,while available potassium,Olsen-phosphorus content,and peroxidase activity continued to increase.However,bacterial alpha diversity(Chao1 and Shannon indices)and the number of amplicon sequence variants increased continuously with planting duration.Principal coordinate analysis and Adonis tests revealed that the planting year significantly influenced the bacterial community structure(p<0.05).The phyla Proteobacteria,Actinobacteria,Acidobacteriota and Chloroflexi collectively constituted 56.7%to 71.2%of the relative abundance,representing the dominant taxa.PICRUSt2 predictions indicated key functional categories(cellular processes,metabolism,genetic information processing,and environmental information processing)each exceeding 10%relative abundance.BugBase analysis revealed a progressive increase in aerobic and oxidative stress-tolerant bacteria and a decrease in anaerobic and potentially pathogenic bacteria.Differential indicator species analysis identified Firmicutes,Planctomycetes,Methylomirabilota and Actinobacteriota as key discriminators for the 1-,5-,10-and 15-year stages,respectively.Organic matter,alkaline phosphatase,soil pH,and available phosphorus were the primary physicochemical drivers of bacterial communities.Notably,soil organic matter significantly influenced both the community structure(p<0.05)and predictedmetabolic functions(p<0.05).In conclusion,prolonged planting duration significantly enhanced rhizosphere microbial diversity and functional gene abundance in Z.bungeanumwhile driving the structural succession of bacterial communities dominated by Proteobacteria,Actinobacteria,Acidobacteriota,and Chloroflexi.This ecological shift,characterized by increased aerobic/oxidative-stress taxa and decreased anaerobic/pathogenic bacteria,was primarily regulated by soil organic matter,a key driver shaping both community structure and metabolic functions,ultimately improving soil microecological health.
文摘As an active flow control technology and with the advantages of no moving components, the Sweeping jet actuator has become a hotspot in the field of flow control. However, the linear relationship between oscillation frequency and momentum coefficient in a sweeping jet actuator makes it difficult to determine the dominant factors that affect control effectiveness. Decoupling the oscillation frequency and momentum coefficient, as well as determining the control mechanism, is the focus of studying the sweeping jet actuator. In this study, a novel sweeping jet actuator is designed using synthetic jets instead of feedback channels and applied to the flow separation control of NACA0018 airfoil. This article studies the control effect under three oscillation frequencies of F<sup>+</sup> = f × c/U<sub>∞</sub> = 1, 10, 100 and three momentum coefficients of C<sub>μ</sub> = 0.45%, 0.625%, 0.9%. The numerical results indicate that all three oscillation frequencies have good control effects on flow separation, and the control effect is best when F<sup>+</sup> = 1, with the maximum lift coefficient increasing by approximately 14% compared to the other two cases. And the sweeping jet actuator has a better ability to control flow separation as the momentum coefficient increases. By decoupling the characteristics of the sweeping jet actuator and conducting numerical analysis of the flow control effect, it will promote its better engineering application in the field of flow control. .
