Ulcerative colitis(UC)is an idiopathic,relapsing,and etiologically complicated chronic inflammatory bowel disease.Despite substantial progress in the management of UC,the outcomes of mucosal barrier repair are unsatis...Ulcerative colitis(UC)is an idiopathic,relapsing,and etiologically complicated chronic inflammatory bowel disease.Despite substantial progress in the management of UC,the outcomes of mucosal barrier repair are unsatisfactory.In this study,phillygenin(PHI)treatment alleviated the symptoms of chronic colitis in mice,including body weight loss,severe disease activity index scores,colon shortening,splenomegaly,oxidative stress,and inflammatory response.In particular,PHI treatment ameliorated the tight junction proteins(TJs)reduction,fibrosis,apoptosis,and intestinal stem cell activity,indicating that PHI exerted beneficial effects on the intestinal mucosal barrier in mice with chronic colitis.In the NCM460 cells damage model,dextran sulfate sodium triggered the sequential induction of TJs reduction,fibrosis,and apoptosis.Takeda G protein-coupled receptor-5(TGR5)dysfunction mediated NCM460 cell injury.Moreover,PHI treatment enhanced TJs and suppressed fibrosis and apoptosis to maintain NCM460 cell function,depending on TGR5 activation.PHI promoted TGR5 activation and elevated intracellular cyclic adenosine monophosphate levels in HEK 293T cells transfected with TGR5 expression plasmids.Cellular thermal shift assay and molecular docking studies confirmed that PHI directly binds to TGR5,indicating that PHI is an agonist of TGR5.The process of PERK-eIF2α pathway-mediated endoplasmic reticulum Ca^(2+) release was involved in NCM460 cell injury as well,which was associated with TGR5 dysfunction.When NCM460 cells were pretreated with PHI,the PERK-eIF2α pathway and elevated Ca^(2+) levels were blocked.In conclusion,our study demonstrated a novel mechanism that PHI inhibited the PERK-eIF2α-Ca^(2+) pathway through TGR5 activation to against DSS-induced TJs reduction,fibrosis,and apoptosis.展开更多
The study of vehicular networks has attracted considerable interest in academia and the industry.In the broad area,connected vehicles and autonomous driving are technologies based on wireless data communication betwee...The study of vehicular networks has attracted considerable interest in academia and the industry.In the broad area,connected vehicles and autonomous driving are technologies based on wireless data communication between vehicles or between vehicles and infrastructures.A Vehicle-to-Infrastructure(V2I)system consists of communications and computing over vehicles and related infrastructures.In such a system,wireless sensors are installed in some selected points along roads or driving areas.In autonomous driving,it is crucial for a vehicle to figure out the ideal routes by the communications between its equipped sensors and infrastructures then the vehicle is automatically moving along the routes.In this paper,we propose a Bezier curve based recursive algorithm,which effectively creates routes for vehicles through the communication between the On-Board Unit(OBU)and the Road-Side Units(RSUs).In addition,this approach generates a very low overhead.We conduct simulations to test the proposed algorithm in various situations.The experiment results demonstrate that our algorithm creates almost ideal routes.展开更多
Rotating machine fault signal extraction becomes increasingly important in practical engineering applications.However,fault signals with low signal-to-noise ratios(SNRs)are difficult to extract,especially at the early...Rotating machine fault signal extraction becomes increasingly important in practical engineering applications.However,fault signals with low signal-to-noise ratios(SNRs)are difficult to extract,especially at the early stage of fault diagnosis.In this paper,2D line-defect phononic crystals(PCs)consisting of periodic acrylic tubes with slit are proposed for weak signal detection.The defect band,namely,the formed resonance band of line-defect PCs enables the incident acoustic wave at the resonance frequency to be trapped and enhanced at the resonance cavity.The noise can be filtered by the band gap.As a result,fault signals with high SNRs can be obtained for fault feature extraction.The effectiveness of weak harmonic and periodic impulse signal detection via line-defect PCs are investigated in numerical and experimental studies.All the numerical and experimental results indicate that line-defect PCs can be well used for extracting weak harmonic and periodic impulse signals.This work will provide potential for extracting weak signals in many practical engineering applications.展开更多
基金supported by the National Natural Science Fund of China(Grant Nos.:31800293 and 32370422)Project of Standard for TCM(Grant No.:ZYBZH-Y-JIN-34).
文摘Ulcerative colitis(UC)is an idiopathic,relapsing,and etiologically complicated chronic inflammatory bowel disease.Despite substantial progress in the management of UC,the outcomes of mucosal barrier repair are unsatisfactory.In this study,phillygenin(PHI)treatment alleviated the symptoms of chronic colitis in mice,including body weight loss,severe disease activity index scores,colon shortening,splenomegaly,oxidative stress,and inflammatory response.In particular,PHI treatment ameliorated the tight junction proteins(TJs)reduction,fibrosis,apoptosis,and intestinal stem cell activity,indicating that PHI exerted beneficial effects on the intestinal mucosal barrier in mice with chronic colitis.In the NCM460 cells damage model,dextran sulfate sodium triggered the sequential induction of TJs reduction,fibrosis,and apoptosis.Takeda G protein-coupled receptor-5(TGR5)dysfunction mediated NCM460 cell injury.Moreover,PHI treatment enhanced TJs and suppressed fibrosis and apoptosis to maintain NCM460 cell function,depending on TGR5 activation.PHI promoted TGR5 activation and elevated intracellular cyclic adenosine monophosphate levels in HEK 293T cells transfected with TGR5 expression plasmids.Cellular thermal shift assay and molecular docking studies confirmed that PHI directly binds to TGR5,indicating that PHI is an agonist of TGR5.The process of PERK-eIF2α pathway-mediated endoplasmic reticulum Ca^(2+) release was involved in NCM460 cell injury as well,which was associated with TGR5 dysfunction.When NCM460 cells were pretreated with PHI,the PERK-eIF2α pathway and elevated Ca^(2+) levels were blocked.In conclusion,our study demonstrated a novel mechanism that PHI inhibited the PERK-eIF2α-Ca^(2+) pathway through TGR5 activation to against DSS-induced TJs reduction,fibrosis,and apoptosis.
基金the Presidential Incentive Awards(No.1103 and No.1105)MCCB summer research award in the University of North Georgia.
文摘The study of vehicular networks has attracted considerable interest in academia and the industry.In the broad area,connected vehicles and autonomous driving are technologies based on wireless data communication between vehicles or between vehicles and infrastructures.A Vehicle-to-Infrastructure(V2I)system consists of communications and computing over vehicles and related infrastructures.In such a system,wireless sensors are installed in some selected points along roads or driving areas.In autonomous driving,it is crucial for a vehicle to figure out the ideal routes by the communications between its equipped sensors and infrastructures then the vehicle is automatically moving along the routes.In this paper,we propose a Bezier curve based recursive algorithm,which effectively creates routes for vehicles through the communication between the On-Board Unit(OBU)and the Road-Side Units(RSUs).In addition,this approach generates a very low overhead.We conduct simulations to test the proposed algorithm in various situations.The experiment results demonstrate that our algorithm creates almost ideal routes.
基金This paper was financially supported by the National Natural Science Foundation of China(Grant No.52175087).
文摘Rotating machine fault signal extraction becomes increasingly important in practical engineering applications.However,fault signals with low signal-to-noise ratios(SNRs)are difficult to extract,especially at the early stage of fault diagnosis.In this paper,2D line-defect phononic crystals(PCs)consisting of periodic acrylic tubes with slit are proposed for weak signal detection.The defect band,namely,the formed resonance band of line-defect PCs enables the incident acoustic wave at the resonance frequency to be trapped and enhanced at the resonance cavity.The noise can be filtered by the band gap.As a result,fault signals with high SNRs can be obtained for fault feature extraction.The effectiveness of weak harmonic and periodic impulse signal detection via line-defect PCs are investigated in numerical and experimental studies.All the numerical and experimental results indicate that line-defect PCs can be well used for extracting weak harmonic and periodic impulse signals.This work will provide potential for extracting weak signals in many practical engineering applications.
