Effectively managing bone defects presents significant clinical challenges,where bone marrow mesenchymal stem cells(BMSCs)offer promising potential for bone regeneration.However,delivering BMSCs effectively requires s...Effectively managing bone defects presents significant clinical challenges,where bone marrow mesenchymal stem cells(BMSCs)offer promising potential for bone regeneration.However,delivering BMSCs effectively requires suitable biomaterials.Herein,we introduce,Pep1,a calcium-responsive peptide designed for delivering BMSCs for femur regeneration.Pep1self-assembles to form a pericellular hydrogel in the presence of calcium ions,forming a nanofiber-rich network conductive to BMSCs encapsulation.Important,Pep1 hydrogel supports BMSC activity in 3D cell culture without compromise.In vitro and in vivo studies demonstrate excellent biocompatibility of Pep1 hydrogel.Osteogenic differentiation and q-PCR assays reveal that Pep1 enhances osteogenic differentiation and upregulates bone-related genes expression.Furthermore,In vivo experiments confirm that Pep1 hydrogel encapsulating BMSCs significantly improves femur defect repair.This study underscores Pep1 as a promising biomaterial for calcium-responsive bone tissue regeneration.展开更多
Increasing evidence supports the hypothesis of autologous immune attack in severe aplastic anemia(SAA):the predominant role of activated cytotoxic T cells(CTL)expressing-interferon in inhibiting the growth of bone mar...Increasing evidence supports the hypothesis of autologous immune attack in severe aplastic anemia(SAA):the predominant role of activated cytotoxic T cells(CTL)expressing-interferon in inhibiting the growth of bone marrow(BM)cells,putative autoantigens,and oligoclonal expansion of CD8+T cells.1 For SAA patients,the definitive therapies are immunosuppressive therapy(IST)or hematopoietic stem transplantation(HSCT).展开更多
基金supported by the National Natural Science Foundation of China(32401127)the National Youth Talent Support Program(202309460011)+2 种基金the Natural Science Foundation of Hunan(2024JJ5072)the Science and Technology and Development Foundation of Shenzhen(JCYJ20210324122403010 and JCYJ20210324133611030)the Medical and Health Technology Program of Zhejiang Province(2023KY148)。
文摘Effectively managing bone defects presents significant clinical challenges,where bone marrow mesenchymal stem cells(BMSCs)offer promising potential for bone regeneration.However,delivering BMSCs effectively requires suitable biomaterials.Herein,we introduce,Pep1,a calcium-responsive peptide designed for delivering BMSCs for femur regeneration.Pep1self-assembles to form a pericellular hydrogel in the presence of calcium ions,forming a nanofiber-rich network conductive to BMSCs encapsulation.Important,Pep1 hydrogel supports BMSC activity in 3D cell culture without compromise.In vitro and in vivo studies demonstrate excellent biocompatibility of Pep1 hydrogel.Osteogenic differentiation and q-PCR assays reveal that Pep1 enhances osteogenic differentiation and upregulates bone-related genes expression.Furthermore,In vivo experiments confirm that Pep1 hydrogel encapsulating BMSCs significantly improves femur defect repair.This study underscores Pep1 as a promising biomaterial for calcium-responsive bone tissue regeneration.
基金supported by grants from the Innovative Clinical Technique of Guangzhou,China(No.2019GX04 and 2023C-GX01 to YPZ and SQW,respectively)2019 Annual Research Project of The China Marrow Donor Program(No.CMDP201902 to SQW)+1 种基金Guangzhou Municipal Science and Technology project(China)(No.202002030035 to SQW)the Natural Science Foundation of Guangdong Province,China(No.2018A0303130179 to MZ).
文摘Increasing evidence supports the hypothesis of autologous immune attack in severe aplastic anemia(SAA):the predominant role of activated cytotoxic T cells(CTL)expressing-interferon in inhibiting the growth of bone marrow(BM)cells,putative autoantigens,and oligoclonal expansion of CD8+T cells.1 For SAA patients,the definitive therapies are immunosuppressive therapy(IST)or hematopoietic stem transplantation(HSCT).
