In situ tissue engineering is a powerful strategy for the treatment of bone defects.It could overcome the limitations of traditional bone tissue engineering,which typically involves extensive cell expansion steps,low ...In situ tissue engineering is a powerful strategy for the treatment of bone defects.It could overcome the limitations of traditional bone tissue engineering,which typically involves extensive cell expansion steps,low cell survival rates upon transplantation,and a risk of immuno-rejection.Here,a porous scaffold polycaprolactone(PCL)/decellularized small intestine submucosa(SIS)was fabricated via cryogenic free-form extrusion,followed by surface modification with aptamer and PlGF-2_(123-144)*-fused BMP2(pBMP2).The two bioactive molecules were delivered sequentially.The aptamer Apt19s,which exhibited binding affinity to bone marrow-derived mesenchymal stem cells(BMSCs),was quickly released,facilitating the mobilization and recruitment of host BMSCs.BMP2 fused with a PlGF-2_(123-144)peptide,which showed“super-affinity”to the ECM matrix,was released in a slow and sustained manner,inducing BMSC osteogenic differentiation.In vitro results showed that the sequential release of PCL/SIS-pBMP2-Apt19s promoted cell migration,proliferation,alkaline phosphatase activity,and mRNA expression of osteogenesis-related genes.The in vivo results demonstrated that the sequential release system of PCL/SIS-pBMP2-Apt19s evidently increased bone formation in rat calvarial critical-sized defects compared to the sequential release system of PCL/SIS-BMP2-Apt19s.Thus,the novel delivery system shows potential as an ideal alternative for achieving cell-free scaffold-based bone regeneration in situ.展开更多
As a new generation of medical metal materials,degradable magnesium-based materials have excellent mechanical properties and osteogenic promoting ability,making them promising materials for the treatment of refractory...As a new generation of medical metal materials,degradable magnesium-based materials have excellent mechanical properties and osteogenic promoting ability,making them promising materials for the treatment of refractory bone diseases.Animal models can be used to understand and evaluate the performance of materials in complex physiological environments,providing relevant data for preclinical evaluation of implants and laying the foundation for subsequent clinical studies.To date,many researchers have studied the biocompatibility,degradability and osteogenesis of magnesium-based materials,but there is a lack of review regarding the effects of magnesium-based materials in vivo.In view of the growing interest in these materials,this review briefly describes the properties of magnesium-based materials and focuses on the safety and efficacy of magnesium-based materials in vivo.Various animal models including rats,rabbits,dogs and pigs are covered to better understand and evaluate the progress and future of magnesium-based materials.This literature analysis reveals that the magnesium-based materials have good biocompatibility and osteogenic activity,thus causing no adverse reaction around the implants in vivo,and that they exhibit a beneficial effect in the process of bone repair.In addition,the degradation rate in vivo can also be improved by means of alloying and coating.These encouraging results show a promising future for the use of magnesium-based materials in musculoskeletal disorders.展开更多
Introduction:We developed the method of cervical spinal cord decompression through durotomy followed by duroplasty and analyzed its efficacy.Purpose:To develop a tactic of decompression durotomy and duroplasty for the...Introduction:We developed the method of cervical spinal cord decompression through durotomy followed by duroplasty and analyzed its efficacy.Purpose:To develop a tactic of decompression durotomy and duroplasty for the treatment of severe spinal cord injury(SCI)with extensive edema of the spinal cord and without intramedullary hematoma,and to demonstrate the effectiveness of this method.Methods:From October 2016 to January 2018,17 decompression operations were performed in the cervical spine in patients with SCI.Decompression laminectomy was done without durotomy in the first group of patients.In the second group,duroplasty of the spinal cord was performed after decompression durotomy.A total of 17 patients,16 males(94%)and 1 female(6%),were operated on(ages from 32 to 66 years).The patients were divided into two groups:a control group and an experimental group.We used the ASIA scale for assessing the patients.The mean follow up time is 12 months(8−24 months).Results:The first group,i.e.,the control group consisted of 10 patients who underwent decompression laminectomy without durotomy.The second group,i.e.,the experimental group consisted of 7 patients who underwent durotomy followed by duroplasty.In this group,the positive dynamics were observed in 6 patients.Out of 2 patients with ASIA grade“A”,one showed improvement to ASIA grade“C”,and one improved to ASIA“D”.Two patients with ASIA grade“B”showed recovery to ASIA“D”.Two patients with ASIA grade“C”improved to grade“D”while one patient showed no change from ASIA“C”.Durotomy and duroplasty was effective in the experimental group.Conclusion:The performance of durotomy and duroplasty is an efficient method for the full-scale decompression of the spinal cord and the prevention of edema.This method aims at decreasing intraspinal pressure,as well as preventing ischemia and apoptosis,which is possible for the prevention and treatment of the spinal cord com-partment syndrome or spinal cord intramedullary hypertension.展开更多
基金the National Natural Science Foundation of China(grant numbers 81902219,81672158 and 81873999)the National Key R&D Program of China(2016YFC1100100).
文摘In situ tissue engineering is a powerful strategy for the treatment of bone defects.It could overcome the limitations of traditional bone tissue engineering,which typically involves extensive cell expansion steps,low cell survival rates upon transplantation,and a risk of immuno-rejection.Here,a porous scaffold polycaprolactone(PCL)/decellularized small intestine submucosa(SIS)was fabricated via cryogenic free-form extrusion,followed by surface modification with aptamer and PlGF-2_(123-144)*-fused BMP2(pBMP2).The two bioactive molecules were delivered sequentially.The aptamer Apt19s,which exhibited binding affinity to bone marrow-derived mesenchymal stem cells(BMSCs),was quickly released,facilitating the mobilization and recruitment of host BMSCs.BMP2 fused with a PlGF-2_(123-144)peptide,which showed“super-affinity”to the ECM matrix,was released in a slow and sustained manner,inducing BMSC osteogenic differentiation.In vitro results showed that the sequential release of PCL/SIS-pBMP2-Apt19s promoted cell migration,proliferation,alkaline phosphatase activity,and mRNA expression of osteogenesis-related genes.The in vivo results demonstrated that the sequential release system of PCL/SIS-pBMP2-Apt19s evidently increased bone formation in rat calvarial critical-sized defects compared to the sequential release system of PCL/SIS-BMP2-Apt19s.Thus,the novel delivery system shows potential as an ideal alternative for achieving cell-free scaffold-based bone regeneration in situ.
基金supported by the National Key R&D Program of China(No.2016YFC1100100)the National Natural Science Foundation of China(Nos.81672158,81873999)the Youth Program of the National Natural Science Foundation of China(No.81902219).
文摘As a new generation of medical metal materials,degradable magnesium-based materials have excellent mechanical properties and osteogenic promoting ability,making them promising materials for the treatment of refractory bone diseases.Animal models can be used to understand and evaluate the performance of materials in complex physiological environments,providing relevant data for preclinical evaluation of implants and laying the foundation for subsequent clinical studies.To date,many researchers have studied the biocompatibility,degradability and osteogenesis of magnesium-based materials,but there is a lack of review regarding the effects of magnesium-based materials in vivo.In view of the growing interest in these materials,this review briefly describes the properties of magnesium-based materials and focuses on the safety and efficacy of magnesium-based materials in vivo.Various animal models including rats,rabbits,dogs and pigs are covered to better understand and evaluate the progress and future of magnesium-based materials.This literature analysis reveals that the magnesium-based materials have good biocompatibility and osteogenic activity,thus causing no adverse reaction around the implants in vivo,and that they exhibit a beneficial effect in the process of bone repair.In addition,the degradation rate in vivo can also be improved by means of alloying and coating.These encouraging results show a promising future for the use of magnesium-based materials in musculoskeletal disorders.
基金supported by the National Natural Science Foundation of China(grant Nos.81672158 and 81371939)International Science&Technology Cooperation Program of China(No.2013DFG32690)National Key R&D Program of China(No.2016YFC1100100).
文摘Introduction:We developed the method of cervical spinal cord decompression through durotomy followed by duroplasty and analyzed its efficacy.Purpose:To develop a tactic of decompression durotomy and duroplasty for the treatment of severe spinal cord injury(SCI)with extensive edema of the spinal cord and without intramedullary hematoma,and to demonstrate the effectiveness of this method.Methods:From October 2016 to January 2018,17 decompression operations were performed in the cervical spine in patients with SCI.Decompression laminectomy was done without durotomy in the first group of patients.In the second group,duroplasty of the spinal cord was performed after decompression durotomy.A total of 17 patients,16 males(94%)and 1 female(6%),were operated on(ages from 32 to 66 years).The patients were divided into two groups:a control group and an experimental group.We used the ASIA scale for assessing the patients.The mean follow up time is 12 months(8−24 months).Results:The first group,i.e.,the control group consisted of 10 patients who underwent decompression laminectomy without durotomy.The second group,i.e.,the experimental group consisted of 7 patients who underwent durotomy followed by duroplasty.In this group,the positive dynamics were observed in 6 patients.Out of 2 patients with ASIA grade“A”,one showed improvement to ASIA grade“C”,and one improved to ASIA“D”.Two patients with ASIA grade“B”showed recovery to ASIA“D”.Two patients with ASIA grade“C”improved to grade“D”while one patient showed no change from ASIA“C”.Durotomy and duroplasty was effective in the experimental group.Conclusion:The performance of durotomy and duroplasty is an efficient method for the full-scale decompression of the spinal cord and the prevention of edema.This method aims at decreasing intraspinal pressure,as well as preventing ischemia and apoptosis,which is possible for the prevention and treatment of the spinal cord com-partment syndrome or spinal cord intramedullary hypertension.
