Tendon regeneration is still a great challenge due to its avascular structure and low self-renewal capability.The nitric oxide(NO)therapy emerges as a promising treatment for inducing the regeneration of injured tendo...Tendon regeneration is still a great challenge due to its avascular structure and low self-renewal capability.The nitric oxide(NO)therapy emerges as a promising treatment for inducing the regeneration of injured tendon by angiogenesis.Here,in this study,a system that NO-loaded metal–organic frameworks(MOFs)encapsulated in polycaprolactone(PCL)/gelatin(Gel)aligned coaxial scaffolds(NMPGA)is designed and prepared for tendon repair.In this system,NO is able to be released in vitro at a slow and stable average speed of 1.67 nM h^−1 as long as 15 d without a burst release stage in the initial 48 h.Furthermore,NMPGA can not only improve the tubular formation capability of endothelial cells in vitro but also obviously increase the blood perfusion near the injured tendon in vivo,leading to accelerating the maturity of collagen and recovery of biomechanical strength of the regenerated tendon tissue.As a NO-loaded MOFs therapeutic system,NMPGA can promote tendon regeneration in a shorter healing period with better biomechanical properties in comparison with control group by angiogenesis.Therefore,this study not only provides a promising scaffold for tendon regeneration,but also paves a new way to develop a NO-based therapy for biomedical application in the future.展开更多
As pioneering materials,single atom catalysts(SACs)have demonstrated exceptional potential across a plethora of domains ranging from biomedicine to energy conversion,environmental preservation,and marine energy.The co...As pioneering materials,single atom catalysts(SACs)have demonstrated exceptional potential across a plethora of domains ranging from biomedicine to energy conversion,environmental preservation,and marine energy.The comprehensive review delves into the latest research advancements in the practical applications of SACs,meticulously dissecting their underlying principles,distinctive features,and versatile applications.Tailoring their operational paradigms to suit diverse application contexts,we elaborate on the operational mechanisms of SACs,accentuating their unparalleled catalytic efficacy and structural resilience.The review systematically delineates the design strategies for various SAC variations,encompassing prevalent materials alongside tactics to fortify their adaptability to environmental conditions and ensure enduring operational stability.Delving further,we scrutinized the potential domains,where SACs demonstrate breakthrough potential in biomedical targeted therapy,efficient energy electrocatalysis,and the deep degradation of pollutants by maximizing atomic utilization and controlling the coordination microenvironment.Conclusively,we deliberate on the challenges confronting SACs with regards to their catalytic prowess,proposing future trajectories and methodologies to amplify their ubiquitous deployment and further refine their efficacy in real-world applications.展开更多
The vicious cycle between tumor cell proliferation and bone resorption remarkably elevates the progression and metastasis of bone tumors.Here,we fabricated polyethylene glycol-conjugated alendronate-functionalized and...The vicious cycle between tumor cell proliferation and bone resorption remarkably elevates the progression and metastasis of bone tumors.Here,we fabricated polyethylene glycol-conjugated alendronate-functionalized and chloroquine(CQ)-loaded polydopamine nanoparticles(PPA/CQ)for efficient treatment of bone tumors via breaking the vicious cycle.The nanoparticles were efficiently accumulated to the bone tissues,especially the osteolytic lesions around tumors.CQ released from PPA/CQ inhibited osteoclastogenesis via preventing the degradation of tumor necrosis factor(TNF)receptor-associated receptor 3 to attenuate the osteolysis in bone tumors.On the other hand,CQ blocked the autophagy in cancer cells,resulting in improved photothermal killing of cancer cells.Finally,the in vivo experiment revealed that PPA/CQ-associated treatment efficiently inhibited both tumor growth and osteolysis.This work suggests that autophagy inhibition-associated photothermal therapy could be a promising strategy for treating malignant bone tumors.展开更多
In order to study the interaction among the traction power supply,the train group and the operation dispatching of urban rail transit,a coupling simulation system of power supply system,trains and dispatching manageme...In order to study the interaction among the traction power supply,the train group and the operation dispatching of urban rail transit,a coupling simulation system of power supply system,trains and dispatching management is constructed.In order to solve the problems of different timescales and difficult cooperation operation for related subsystems,a multi-bus distributed real-time network architecture based on hierarchical management of communication data is established,and simulation management software is developed to facilitate the free expansion of the simulation system.Meanwhile,the track line,train operation and other large timescale subsystems are realized by the pure digital simulation.And the time-sensitive subsystems,such as train traction system,braking system,auxiliary power supply system and network system etc.,are built by the semi-physical simulation.In this article,the system structure and the main implementation principle of each simulation subsystem are given in detail,and the system is tested and verified at the end.The results show that the simulation system can meet the expected requirements.展开更多
基金supported by National Key R&D Program of China(2016YFC1100300)National Natural Science Foundation of China(Nos.81772339,8181101445 and 81972129)+6 种基金The Key Clinical Medicine Center of Shanghai(2017ZZ01006)Sanming Project of Medicine in Shenzhen(SZSM201612078)Shanghai Rising-Star Project(18QB1400500)the Natural Science Foundation of Shanghai(No.19ZR1437800)The Introduction Project of Clinical Medicine Expert Team for Suzhou(SZYJTD201714)Development Project of Shanghai Peak Disciplines-Integrative Medicine(20180101)Shanghai Committee of Science and Technology(19441901600 and 19441902000).
文摘Tendon regeneration is still a great challenge due to its avascular structure and low self-renewal capability.The nitric oxide(NO)therapy emerges as a promising treatment for inducing the regeneration of injured tendon by angiogenesis.Here,in this study,a system that NO-loaded metal–organic frameworks(MOFs)encapsulated in polycaprolactone(PCL)/gelatin(Gel)aligned coaxial scaffolds(NMPGA)is designed and prepared for tendon repair.In this system,NO is able to be released in vitro at a slow and stable average speed of 1.67 nM h^−1 as long as 15 d without a burst release stage in the initial 48 h.Furthermore,NMPGA can not only improve the tubular formation capability of endothelial cells in vitro but also obviously increase the blood perfusion near the injured tendon in vivo,leading to accelerating the maturity of collagen and recovery of biomechanical strength of the regenerated tendon tissue.As a NO-loaded MOFs therapeutic system,NMPGA can promote tendon regeneration in a shorter healing period with better biomechanical properties in comparison with control group by angiogenesis.Therefore,this study not only provides a promising scaffold for tendon regeneration,but also paves a new way to develop a NO-based therapy for biomedical application in the future.
基金supported by the Fundamental Research Funds for the Central Universities(No.22120220635)the Special Funds of the Tongji University for“Sino-German Cooperation 2.0 Strategy”(No.ZD2023026)+5 种基金the National Key R&D Program of China(No.2022YFF1202600)the National Natural Science Foundation of China(No.22305101)the Basic Research Program of Jiangsu(No.BK20231032)the Health Industry Clinical Research Project of Shanghai Health Commission(No.20224Y0393)the Science and Technology Development Fund of Shanghai Pudong New Area(No.PKJ2023-Y09)the Outstanding Leaders Training Program of Pudong Hospital affiliated to Fudan University(No.LX202201).
文摘As pioneering materials,single atom catalysts(SACs)have demonstrated exceptional potential across a plethora of domains ranging from biomedicine to energy conversion,environmental preservation,and marine energy.The comprehensive review delves into the latest research advancements in the practical applications of SACs,meticulously dissecting their underlying principles,distinctive features,and versatile applications.Tailoring their operational paradigms to suit diverse application contexts,we elaborate on the operational mechanisms of SACs,accentuating their unparalleled catalytic efficacy and structural resilience.The review systematically delineates the design strategies for various SAC variations,encompassing prevalent materials alongside tactics to fortify their adaptability to environmental conditions and ensure enduring operational stability.Delving further,we scrutinized the potential domains,where SACs demonstrate breakthrough potential in biomedical targeted therapy,efficient energy electrocatalysis,and the deep degradation of pollutants by maximizing atomic utilization and controlling the coordination microenvironment.Conclusively,we deliberate on the challenges confronting SACs with regards to their catalytic prowess,proposing future trajectories and methodologies to amplify their ubiquitous deployment and further refine their efficacy in real-world applications.
基金the National Natural Science Foundation of China(21725402,31871010,81971735,81871470 and 81901867)Shanghai Municipal Science and Technology Commission(17XD1401600)+1 种基金the Fok Ying Tong Education Foundation(151036)Guangdong Innovative and Entrepreneurial Research Team Program(2016ZT06C322)。
文摘The vicious cycle between tumor cell proliferation and bone resorption remarkably elevates the progression and metastasis of bone tumors.Here,we fabricated polyethylene glycol-conjugated alendronate-functionalized and chloroquine(CQ)-loaded polydopamine nanoparticles(PPA/CQ)for efficient treatment of bone tumors via breaking the vicious cycle.The nanoparticles were efficiently accumulated to the bone tissues,especially the osteolytic lesions around tumors.CQ released from PPA/CQ inhibited osteoclastogenesis via preventing the degradation of tumor necrosis factor(TNF)receptor-associated receptor 3 to attenuate the osteolysis in bone tumors.On the other hand,CQ blocked the autophagy in cancer cells,resulting in improved photothermal killing of cancer cells.Finally,the in vivo experiment revealed that PPA/CQ-associated treatment efficiently inhibited both tumor growth and osteolysis.This work suggests that autophagy inhibition-associated photothermal therapy could be a promising strategy for treating malignant bone tumors.
文摘In order to study the interaction among the traction power supply,the train group and the operation dispatching of urban rail transit,a coupling simulation system of power supply system,trains and dispatching management is constructed.In order to solve the problems of different timescales and difficult cooperation operation for related subsystems,a multi-bus distributed real-time network architecture based on hierarchical management of communication data is established,and simulation management software is developed to facilitate the free expansion of the simulation system.Meanwhile,the track line,train operation and other large timescale subsystems are realized by the pure digital simulation.And the time-sensitive subsystems,such as train traction system,braking system,auxiliary power supply system and network system etc.,are built by the semi-physical simulation.In this article,the system structure and the main implementation principle of each simulation subsystem are given in detail,and the system is tested and verified at the end.The results show that the simulation system can meet the expected requirements.
