The color difference of capsicum fruit is closely related to the type and content of pigment in the peel,which is mainly determined by anthocyanins,chlorophyll,and carotenoids.This study used green“CA59”and purple“...The color difference of capsicum fruit is closely related to the type and content of pigment in the peel,which is mainly determined by anthocyanins,chlorophyll,and carotenoids.This study used green“CA59”and purple“Z81”pepper fruits as parents to create the F2 generation.The fruit color of 466 F2 population was identified,and the extreme individuals from this population were selected for Bulked Segregant Analysis(BSA)using resequencing.Genetic analysis revealed that a pair of genes controls the expression of the purple fruit trait in capsicum.Using functional annotation,expression analysis,and sequencing analysis of candidate genes,it was determined that there were four genes in the region between InDel 67 and InDel 75(185,664,068 BP-186,514,350 bp)on chromosome 10,that is the linkage interval for pepper purple fruit.There are 7 SNPs in the CaMYB1 gene(Capann_59V1aChr10g016200)in the pepper variety“Z81”.Of these,4 SNPs are located in the gene’s coding region.These 4 SNPs lead to 2 mutations that do not change the amino acid sequence(synonymous mutations)and 2 mutations that do change the amino acid sequence(non-synonymous mutations).Additionally,the expression level of the CaMYB1 gene in the purple fruit of“Z81”is significantly higher than that in the green fruit of“CA59”.CaMYB1 is believed to be a crucial candidate gene in regulating anthocyanin production in purple capsicum fruit.A molecular marker,InDel 67,was successfully developed,with a total separation rate of 92.4%.展开更多
BACKGROUND Gastric cancer(GC)is a common type of digestive cancer with high morbidity and mortality rates worldwide.Considerable effort has been expended in understanding the mechanism of GC development and metastasis...BACKGROUND Gastric cancer(GC)is a common type of digestive cancer with high morbidity and mortality rates worldwide.Considerable effort has been expended in understanding the mechanism of GC development and metastasis.The current study therefore explores the involvement of microRNAs in the regulation of GC progression.AIM To explore the expression and function of miR-30e-3p in GC development.METHODS MiR-30e-3p was found to be downregulated in GC,with low levels thereof predicting poor outcomes among patients with GC.Functionally,we revealed that miR-30e-3p suppressed cell growth and metastatic behaviors of GC cells.Bioinformatics analysis predicted that THO complex 2(THOC2)was a direct target of miR-30e-3p,and the interaction between miR-30e-3p and THOC2 was further validated by a luciferase reporter assay.RESULTS Our findings revealed that knockdown of THOC2 inhibited the growth and metastatic behaviors of GC cells.After investigating signaling pathways involved in miR-30e-3p regulation,we found that the miR-30e-3p/THOC2 axis regulated the PI3K/AKT/mTOR pathway in GC.CONCLUSION Our findings suggest the novel functional axis miR-30e-3p/THOC2 is involved in GC development and progression.The miR-30e-3p/THOC2 axis could be utilized to develop new therapies against GC.展开更多
Ions in the bulk of solvent-free ionic liquids bind into ion pairs and clusters.The competition between the propensity of ions to stay in a bound state,and the reduction of the energy when unbinding in electric field,...Ions in the bulk of solvent-free ionic liquids bind into ion pairs and clusters.The competition between the propensity of ions to stay in a bound state,and the reduction of the energy when unbinding in electric field,determines the portion of free ions in the electrical double layer.We present the simplest possible mean-field theory to study this effect."Cracking"of ion pairs into free ions in electric field is accompanied by the change of the dielectric response of the ionic liquid.The predictions from the theory are verified and further explored by molecular dynamics simulations.A particular finding of the theory is that the differential capacitance vs potential curve displays a bell shape,despite the low concentration of free charge carriers,because the dielectric response reduces the threshold concentration for the bell-to camelshape transition.The presented theory does not take into account overscreening and oscillating charge distributions in the electrical double layer.But in spite of the simplicity of the model,its findings demonstrate a clear physical effect:a preference to be a charged monopole rather than a dipole(or higher order multipole)in strong electric field.展开更多
Insulation failure significantly contributes to the unpredictable shutdown of power equipment.Compared to the partial discharge and high-frequency(HF)injection methods,the HF common-mode(CM)leakage current method offe...Insulation failure significantly contributes to the unpredictable shutdown of power equipment.Compared to the partial discharge and high-frequency(HF)injection methods,the HF common-mode(CM)leakage current method offers a non-intrusive and highly sensitive alternative.However,the detection of HF CM currents is susceptible to interference from differential-mode(DM)currents,which exhibit high-amplitude and multifrequency components during normal operation.To address this challenge,this paper proposes a double-ring current sensor based on the principle of magnetic shielding for inverter-fed machine winding insulation monitoring.The inner ring harnesses the magnetic aggregation effect to isolate the DM current magnetic field,whereas the outer ring serves as the magnetic core of the Rogowski current sensor,enabling HF CM current monitoring.First,the magnetic field distributions of the CM and DM currents were analyzed.Then,a correlation between the sensor parameters and signal-to-noise ratio of the target HF CM current was established.Finally,an experimental study was conducted on a 3-kW PMSM for verification.The results indicate that the proposed double-ring HF CM sensor can effectively mitigate DM current interference.Compared to a single-ring sensor,a reduction of approximately 40%in the DM component was achieved,which significantly enhanced the precision of online insulation monitoring.展开更多
BACKGROUND Liver cancer is a malignant tumor with high morbidity and mortality.Transcatheter arterial chemoembolization(TACE)is the main method for surgically unresectable liver cancer.In recent years,drug-loaded micr...BACKGROUND Liver cancer is a malignant tumor with high morbidity and mortality.Transcatheter arterial chemoembolization(TACE)is the main method for surgically unresectable liver cancer.In recent years,drug-loaded microspheres have been gradually applied in TACE technology.There are some controversies about the therapeutic effects of drug-loaded microspheres TACE(D-TACE)and traditional TACE.AIM To explore the short-term efficacy of D-TACE and traditional TACE in the treatment of advanced liver cancer.METHODS The clinical data of 73 patients with advanced liver cancer admitted to the First and Sixth Medical Centers of Chinese PLA General Hospital from January 2017 to October 2019 were retrospectively analyzed.Among them,15 patients were treated with D-TACE,and 58 patients were treated with traditional TACE.Clinical baseline characteristics,perioperative laboratory indices,postoperative adverse reactions and postoperative complications were compared between the two groups.RESULTS There was no statistical difference between the two groups for the postoperative response:The highest postoperative body temperature of the drug-loaded microsphere group was 38.0±0.9℃and the postoperative highest body temperature of the traditional TACE group was 38.3±0.7℃(t=-1.414,P=0.162).For the 24 h postoperative nausea and vomiting after surgery in terms of scoring and postoperative pain scores,the traditional TACE group was higher than the drugloaded microsphere group(χ2=14.33,P=0.014;χ2=32.967,P=0.000)and the two groups had significant statistical differences.The disease control rate at 3 mo after treatment in the drugloaded microsphere group was 60%and the disease control rate at 3 mo after treatment in the traditional TACE group was 75.9%(χ2=4.091,P=0.252).There was no statistical difference between the two groups of data.During the follow-up period,the number of interventional treatments received was once in the drug-loaded microsphere group and the traditional TACE group received an average of 1.48 treatments(χ2=10.444 P=0.005).There was a statistical difference between the two groups.CONCLUSION Compared with traditional TACE,D-TACE may have some advantages in the treatment of advanced hepatocellular carcinoma with a large tumor load in the short term,but the long-term clinical efficacy needs additional follow-up studies.In addition,compared with the traditional group,the patients in the drug-loaded microsphere group had better subjective tolerance and could reduce the number of interventional treatments.Therefore,D-TACE is worthy of clinical promotion.展开更多
Acoustic array sensor device for partial discharge detection is widely used in power equipment inspection with the advantages of non-contact and precise positioning compared with partial discharge detection methods su...Acoustic array sensor device for partial discharge detection is widely used in power equipment inspection with the advantages of non-contact and precise positioning compared with partial discharge detection methods such as ultrasonic method and pulse current method.However,due to the sensitivity of the acoustic array sensor and the influence of the equipment operation site interference,the acoustic array sensor device for partial discharge type diagnosis by phase resolved partial discharge(PRPD)map might occasionally presents incorrect results,thus affecting the power equipment operation and maintenance strategy.The acoustic array sensor detection device for power equipment developed in this paper applies the array design model of equal-area multi-arm spiral with machine learning fast fourier transform clean(FFT-CLEAN)sound source localization identification algorithm to avoid the interference factors in the noise acquisition system using a single microphone and conventional beam forming algorithm,improves the spatial resolution of the acoustic array sensor device,and proposes an acoustic array sensor device based on the acoustic spectrogram.The analysis and diagnosis method of discharge type of acoustic array sensor device can effectively reduce the system misjudgment caused by factors such as the resolution of the acoustic imaging device and the time domain pulse of the digital signal,and reduce the false alarm rate of the acoustic array sensor device.The proposed method is tested by selecting power cables as the object,and its effectiveness is proved by laboratory verification and field verification.展开更多
The pulse current method,acoustic and ultrasonic partial discharge(PD)detection,and voiceprint PD detection are commonly used detection methods for the PD detection of power equipment.To study the characteristics of P...The pulse current method,acoustic and ultrasonic partial discharge(PD)detection,and voiceprint PD detection are commonly used detection methods for the PD detection of power equipment.To study the characteristics of PD signals of typical discharge models based on the principles of the above three detection methods,an acoustic detection experimental system consisting of a needle-tip model and a surface model was built.Acoustic tests were carried out on needle-tip models with different curvature radii and surface discharge models with different lengths of conductive paste.The experimental results showed that acoustic and ultrasonic PD detection and voiceprint PD detection exhibited different sensitivities to the needle-tip discharge models,and the combination of acoustic and ultrasonic PD and voiceprint PD detection was more beneficial for the comprehensive detection of cable PD signals.Based on voiceprint recognition technology,this study drew FFT(Fast Fourier Transformation)diagrams of different types of PD acoustic signals and analyzed the differences in the ultrasonic signal frequency distribution.The frequency band of the voiceprint PD signal of the needle-tip discharge models was concentrated in the range 17-27 kHz,and the frequency band of the voiceprint PD signal of the conductive paste discharge models was concentrated in the range 20-25 kHz.The measurement of voiceprint PD signals in these frequency bands were strengthened when the PD of a cable was detected on-site,which provides the basis for the use of the cable model for on-site PD detection.展开更多
Objective:To investigate the biochemical constituents of the fruits of Terminalia bellerica(Gaertn.)Roxb.(hereafter termed T.bellerica)and estimate the anti-cancer activity of different polar extracts.Methods:To rapid...Objective:To investigate the biochemical constituents of the fruits of Terminalia bellerica(Gaertn.)Roxb.(hereafter termed T.bellerica)and estimate the anti-cancer activity of different polar extracts.Methods:To rapidly screen and identify the biochemical constituents of ethyl acetate(EA)extracts of T.bellerica,ultra performance liquid chromatography-electrospray ionization/mass spectrometry(UPLC-ESI-MSn)was done.The CellTiter-BlueTM cell-viability assay was used to ascertain the anti-cancer activity of different polar extracts in 10 human cancer cell lines.Results:Forty polyphenols of the EA extract of T.bellerica were characterized tentatively.The EA extract exhibited significant anti-cancer activity against ZR-75-1 cells(half-maximal inhibitory concentration=27.33(0.98)μg/mL)and Colo-205 cells(39.65(2.99)μg/mL)in vitro.Treatment of ZR-75-1 cells with 20 and 60μg/mL of the EA extract elicited dosedependent apoptosis percentages at an early stage of 17.58(0.74)%and at a late stage of 29.20(1.22)%;Colo-205 cells at the same concentration of EA extract had values of 21.33(1.03)%and 40.55(0.34)%,respectively.Western blotting suggested that ZR-75-1 and Colo205 cells treated with the EA extract showed a similar increasing tendency for expression of cleaved anti-poly adenosine diphosphate ribose polymerase I.Conclusion:We identified a total of 40 chemical constituents,of which 11 were first obtained from the Terminalia Linn.genus using UPLC-ESI-MSn.Meanwhile,we observe that the EA extract of T.bellerica possesses anti-cancer activity,especially against breast and colon cancers.展开更多
Virtual power plants can effectively integrate different types of distributed energy resources,which have become a new operation mode with substantial advantages such as high flexibility,adaptability,and economy.This ...Virtual power plants can effectively integrate different types of distributed energy resources,which have become a new operation mode with substantial advantages such as high flexibility,adaptability,and economy.This paper proposes a distributionally robust optimal dispatch approach for virtual power plants to determine an optimal day-ahead dispatch under uncertainties of renewable energy sources.The proposed distributionally robust approach characterizes probability distributions of renewable power output by moments.In this regard,the faults of stochastic optimization and traditional robust optimization can be overcome.Firstly,a second-order cone-based ambiguity set that incorporates the first and second moments of renewable power output is constructed,and a day-ahead two-stage distributionally robust optimization model is proposed for virtual power plants participating in day-ahead electricity markets.Then,an effective solution method based on the affine policy and second-order cone duality theory is employed to reformulate the proposed model into a deterministic mixed-integer second-order cone programming problem,which improves the computational efficiency of the model.Finally,the numerical results demonstrate that the proposed method achieves a better balance between robustness and economy.They also validate that the dispatch strategy of virtual power plants can be adjusted to reduce costs according to the moment information of renewable power output.展开更多
[Objectives] This study was conducted to strengthen the understanding of the variation laws of pH in fishery waters. [Methods]Based on the investigation data of pH, dissolved oxygen(Do), water temperature(T) and chlor...[Objectives] This study was conducted to strengthen the understanding of the variation laws of pH in fishery waters. [Methods]Based on the investigation data of pH, dissolved oxygen(Do), water temperature(T) and chlorophyll a(Chl. a) of the fishery waters in Quzhou City from 2017 to 2020, the seasonal variation characteristics and influencing factors of pH in the fishery waters were analyzed and discussed. [Results] The pH of the fishery waters in Quzhou City was in the range of 6.40-11.3, and was characterized by the highest seasonal variation in spring and the lowest in winter. The correlation analysis showed that water temperature and pH were significantly positively correlated in spring and summer(P<0.05), and the photosynthesis of phytoplankton was the main reason that affected the pH of water bodies in spring and summer. There was a negative correlation between water temperature and pH in winter, and the pH in the natural waters had a significant negative correlation with water temperature(P<0.05). The balance of carbonate system in the water bodies was the main reason that affected the pH of water in winter. The level of water eutrophication(Chl. a) affected the pH of fishery waters to a certain extent. When Chl. a<10 μg/L, there was a significant positive correlation between the pH of fishery waters and Chl. a(P<0.05). Various environmental factors had different effects on the pH of different types of fishery waters. The stepwise regression analysis showed that the pH of natural waters was mainly affected by Chl. a, and the pH of aquaculture waters was mainly affected by water temperature and dissolved oxygen. [Conclusions]This study provides certain theoretical support for actual production management.展开更多
[Objectives]This study was conducted to construct a pColdI-HSP70 recombinant prokaryotic expression vector.[Methods]With rice‘N22’as the test variety,hydroponic experiments were set up for rice seedlings with normal...[Objectives]This study was conducted to construct a pColdI-HSP70 recombinant prokaryotic expression vector.[Methods]With rice‘N22’as the test variety,hydroponic experiments were set up for rice seedlings with normal growth(CK),high temperature treatment(H),drought treatment(D)and drought-high temperature cross treatment(DH).The recombinant prokaryotic expression vector was constructed by the method of prokaryotic expression,and its induction expression time,IPTG concentration and temperature were optimized.[Results]The pColdI-HSP70 expression vector was successfully constructed,and the fusion protein was highly expressed in host strain BL21,and the expressed proteins were all in a soluble form.By optimizing the induction expression conditions,it was found that the optimal expression conditions were the IPTG concentration of 0.5 mmol/L and induction at 20℃for 36 h.The expression analysis of the rice HSP70 gene under different stress treatments was carried out by qRT-PCR technology,and it was found that H,D and DH stresses all could induce its expression,and its expression levels were 4.65,1.40 and 17.66 times higher than that of the CK group,respectively.[Conclusions]This study lays a solid foundation for the isolation,purification and functional study of rice HSP70 proteins.展开更多
BACKGROUND We invented Endoscopic Ruler,a new endoscopic device to measure the size of varices in patients with cirrhosis and portal hypertension.AIM To assess the feasibility and safety of Endoscopic Ruler,and evalua...BACKGROUND We invented Endoscopic Ruler,a new endoscopic device to measure the size of varices in patients with cirrhosis and portal hypertension.AIM To assess the feasibility and safety of Endoscopic Ruler,and evaluate the agreement on identifying large oesophageal varices(OV)between Endoscopic Ruler and the endoscopists,as well as the interobserver agreement on diagnosing large OV using Endoscopic Ruler.METHODS We prospectively and consecutively enrolled patients with cirrhosis from 11 hospitals,all of whom got esophagogastroduodenoscopy(EGD)with Endoscopic Ruler.The primary study outcome was a successful measurement of the size of varices using Endoscopic Ruler.The secondary outcomes included adverse events,operation time,the agreement of identifying large OV between the objective measurement of Endoscopic Ruler and the empirical reading of endoscopists,together with the interobserver agreement on diagnosing large OV by Endoscopic Ruler.RESULTS From November 2020 to April 2022,a total of 120 eligible patients with cirrhosis were recruited and all of them underwent EGD examinations with Endoscopic Ruler successfully without any adverse event.The median operation time of Endoscopic Ruler was 3.00 min[interquartile range(IQR):3.00 min].The kappa value between Endoscopic Ruler and the endoscopists while detecting large OV was 0.52,demonstrating a moderate agreement.The kappa value for diagnosing large OV using Endoscopic Ruler among the six independent observers was 0.77,demonstrating a substantial agreement.CONCLUSION The data demonstrates that Endoscopic Ruler is feasible and safe for measuring the size of varices in patients with cirrhosis and portal hypertension.Endoscopic Ruler is potential to promote the clinical practice of the two-grade classification system of OV.展开更多
Colitis is a chronic bowel disease characterized by damage to the lining of the large intestine,with its precise underlying causes remaining incompletely understood.In this study,we provide evidence that circular RNA ...Colitis is a chronic bowel disease characterized by damage to the lining of the large intestine,with its precise underlying causes remaining incompletely understood.In this study,we provide evidence that circular RNA circNlgn plays a pivotal role in promoting the development of colitis.Colitis patients produce significant higher levels of circNign.Transgenic mice expressing circNlgn exhibit heightened susceptibility to colitis development and progression,primarily attributed to the presence of the protein isoform Nlgn173 encoded by circNign.Nlgn173 undergoes translocation into cellnuclei,where it interacts with actin,impeding the binding of actin-related protein 2 and 3(Arp2/3)complex to actin molecules.Consequently,this leads to a reduction in actin polymerization.Mechanistically,Nlgn173 enhances tyrosine-53 phosphorylation of nuclear actin,diminishing its capacity to interact with the Arp2/3 complex and causing a decrease in filamentous actin levels.These alterations in actin dynamics result in inhibited cell cycle progression,increased apoptosis,and decreased proliferation of colonic epithelial cells,thereby exacerbating colitis development and progression.In contrast,the silencing of circNign or the targeted inhibition of Nlgn173 translation and nuclear translocation leads to the promotion of nuclear actin polymerization,enhanced cell survival,and reduced apoptosis and ultimately improves the outcome of colitis in vivo.Interestingly,nuclear actin polymerization is highly related with expression of PIAS3,which modulates signal transducer and activator of transcription 3 and NF-kB activity in colitis.Strategies such as circNlgn knockdown and targeting nuclear actin polymerization of the colonic epithelium may explore a novel avenue for acute ulcerativecolitisclinical intervention.展开更多
Recent large-scale multi-omics studies have characterized the heterogeneity of esophageal squamous cell carcinoma(ESCC),but inconsistent clinical management has hindered the identification of prognostic markers and pa...Recent large-scale multi-omics studies have characterized the heterogeneity of esophageal squamous cell carcinoma(ESCC),but inconsistent clinical management has hindered the identification of prognostic markers and patient stratification.Here,we conducted genomic and transcriptomic profiling of 203 patients from the ECTOP-2002 study with full clinical information.Mutation in the mucin family,as well as APOBEC signature,were associated with poor prognosis.In contrast,activation of the epithelial-keratinization(EpK)pathway was strongly linked to favorable prognosis and lower post-chemotherapy recurrence rates.Independent validation supported S100A8+S100A9 complex as a key marker of EpK pathway.Furthermore,we established a prognostic stratification system,FU-ESCC subtyping,which defines three subtypes with distinct molecular and clinical features.The EpK-activated subtype retained characteristics of healthy squamous epithelial cells,showed high expression of the S100A8+S100A9 complex,and was associated with favorable prognosis.展开更多
Self-healing polyurethane-elastomers are highly desired in various fields.However,there is often a trade-off between mechanical properties and dynamic self-healing due to the mutually exclusive mechanism.Herein,we dev...Self-healing polyurethane-elastomers are highly desired in various fields.However,there is often a trade-off between mechanical properties and dynamic self-healing due to the mutually exclusive mechanism.Herein,we develop a self-healable and mechanically robust poly(oxime-urethane)elastomer(Zn-DAPU)to circumvent this inherent trade-off by incorporating zincpyridinyl cross-links into the hydrogen bonding and dynamic oxime-urethane supramolecular-covalent hybrid network.Benefiting from the synergistic strengthening of H-bonding and coordinate interactions,Zn-DAPU network performs tunable toughness with metal ion concentration change,which improves 345.2%and reaches 82.2 MJ m^(-3),with robust tensile strength of 22.8 MPa,Young's modulus of 37.1 MPa,and satisfactory elongation of 815.7%.The healing efficiency can be reached at 91.7%with a restored toughness of 75.4 MJ m^(-3)at 80℃for 10 h.Furthermore,zinc-contained networks exhibit photolysis behavior due to the homolytic cleavage of N-O bonds in oxime-urethane moieties,which can be functionalized further with fluorescamine as the specific information encryption coating with quick response codes(QRs)upon polyester fabric.This work provides valuable guidance towards the development of high-performance self-healing polyurethane and wearable functional materials.展开更多
Covariate-adaptive randomisation has a long history of applications in clinical trials. Shao, Yu,and Zhong [(2010). A theory for testing hypotheses under covariate-adaptive randomization.Biometrika, 97, 347–360] and ...Covariate-adaptive randomisation has a long history of applications in clinical trials. Shao, Yu,and Zhong [(2010). A theory for testing hypotheses under covariate-adaptive randomization.Biometrika, 97, 347–360] and Shao and Yu [(2013). Validity of tests under covariate-adaptivebiased coin randomization and generalized linear models. Biometrics, 69, 960–969] showed thatthe simple t-test is conservative under covariate-adaptive biased coin (CABC) randomisation interms of type I error, and proposed a valid test using the bootstrap. Under a general additivemodel with CABC randomisation, we construct a calibrated t-test that shares the same propertyas the bootstrap method in Shao et al. (2010), but do not need large computation required by thebootstrap method. Some simulation results are presented to show the finite sample performanceof the calibrated t-test.展开更多
基金supported by the National Natural Science Foundation of China(U2IA20230)the Guangdong Modern Vegetable Industry Technology System Project(2024CXTD08)+2 种基金the Guangdong Basic and Applied Basic Research Foundation(2024A1515010403)the Xizang Autonomous Region of Lhasa City Science and Technology Project(LSKJ202418)the Guangdong Provincial Department of Agriculture and Rural Affairs(Selection and Breeding of New High-Yielding and High-Quality Pepper Varieties)(2024-NPY-00-020).
文摘The color difference of capsicum fruit is closely related to the type and content of pigment in the peel,which is mainly determined by anthocyanins,chlorophyll,and carotenoids.This study used green“CA59”and purple“Z81”pepper fruits as parents to create the F2 generation.The fruit color of 466 F2 population was identified,and the extreme individuals from this population were selected for Bulked Segregant Analysis(BSA)using resequencing.Genetic analysis revealed that a pair of genes controls the expression of the purple fruit trait in capsicum.Using functional annotation,expression analysis,and sequencing analysis of candidate genes,it was determined that there were four genes in the region between InDel 67 and InDel 75(185,664,068 BP-186,514,350 bp)on chromosome 10,that is the linkage interval for pepper purple fruit.There are 7 SNPs in the CaMYB1 gene(Capann_59V1aChr10g016200)in the pepper variety“Z81”.Of these,4 SNPs are located in the gene’s coding region.These 4 SNPs lead to 2 mutations that do not change the amino acid sequence(synonymous mutations)and 2 mutations that do change the amino acid sequence(non-synonymous mutations).Additionally,the expression level of the CaMYB1 gene in the purple fruit of“Z81”is significantly higher than that in the green fruit of“CA59”.CaMYB1 is believed to be a crucial candidate gene in regulating anthocyanin production in purple capsicum fruit.A molecular marker,InDel 67,was successfully developed,with a total separation rate of 92.4%.
基金Supported by National Natural Science Foundation of China,No.81860442Ningxia Natural Science Foundation Project,No.2022AAC03525.
文摘BACKGROUND Gastric cancer(GC)is a common type of digestive cancer with high morbidity and mortality rates worldwide.Considerable effort has been expended in understanding the mechanism of GC development and metastasis.The current study therefore explores the involvement of microRNAs in the regulation of GC progression.AIM To explore the expression and function of miR-30e-3p in GC development.METHODS MiR-30e-3p was found to be downregulated in GC,with low levels thereof predicting poor outcomes among patients with GC.Functionally,we revealed that miR-30e-3p suppressed cell growth and metastatic behaviors of GC cells.Bioinformatics analysis predicted that THO complex 2(THOC2)was a direct target of miR-30e-3p,and the interaction between miR-30e-3p and THOC2 was further validated by a luciferase reporter assay.RESULTS Our findings revealed that knockdown of THOC2 inhibited the growth and metastatic behaviors of GC cells.After investigating signaling pathways involved in miR-30e-3p regulation,we found that the miR-30e-3p/THOC2 axis regulated the PI3K/AKT/mTOR pathway in GC.CONCLUSION Our findings suggest the novel functional axis miR-30e-3p/THOC2 is involved in GC development and progression.The miR-30e-3p/THOC2 axis could be utilized to develop new therapies against GC.
基金funding support from the National Natural Science Foundation of China(51876072)financial support from National Natural Science Foundation of China(21802170)+2 种基金supported through a studentship of the Centre for Doctoral Training on Theory and Simulation of Materials at Imperial College London,funded by the EPSRC(EP/L015579/1)the funding from the Thomas Young Centre under grant number TYC-101funding from the Leverhulme Trust(Grant No.RPG2016-223)
文摘Ions in the bulk of solvent-free ionic liquids bind into ion pairs and clusters.The competition between the propensity of ions to stay in a bound state,and the reduction of the energy when unbinding in electric field,determines the portion of free ions in the electrical double layer.We present the simplest possible mean-field theory to study this effect."Cracking"of ion pairs into free ions in electric field is accompanied by the change of the dielectric response of the ionic liquid.The predictions from the theory are verified and further explored by molecular dynamics simulations.A particular finding of the theory is that the differential capacitance vs potential curve displays a bell shape,despite the low concentration of free charge carriers,because the dielectric response reduces the threshold concentration for the bell-to camelshape transition.The presented theory does not take into account overscreening and oscillating charge distributions in the electrical double layer.But in spite of the simplicity of the model,its findings demonstrate a clear physical effect:a preference to be a charged monopole rather than a dipole(or higher order multipole)in strong electric field.
基金supported in part by the National Natural Science Foundation of China under Grant 51907116in part sponsored by Natural Science Foundation of Shanghai 22ZR1425400sponsored by Shanghai Rising-Star Program 23QA1404000。
文摘Insulation failure significantly contributes to the unpredictable shutdown of power equipment.Compared to the partial discharge and high-frequency(HF)injection methods,the HF common-mode(CM)leakage current method offers a non-intrusive and highly sensitive alternative.However,the detection of HF CM currents is susceptible to interference from differential-mode(DM)currents,which exhibit high-amplitude and multifrequency components during normal operation.To address this challenge,this paper proposes a double-ring current sensor based on the principle of magnetic shielding for inverter-fed machine winding insulation monitoring.The inner ring harnesses the magnetic aggregation effect to isolate the DM current magnetic field,whereas the outer ring serves as the magnetic core of the Rogowski current sensor,enabling HF CM current monitoring.First,the magnetic field distributions of the CM and DM currents were analyzed.Then,a correlation between the sensor parameters and signal-to-noise ratio of the target HF CM current was established.Finally,an experimental study was conducted on a 3-kW PMSM for verification.The results indicate that the proposed double-ring HF CM sensor can effectively mitigate DM current interference.Compared to a single-ring sensor,a reduction of approximately 40%in the DM component was achieved,which significantly enhanced the precision of online insulation monitoring.
基金National key research and development project of Ministry of Science and Technology,No.2016YFC0103908.
文摘BACKGROUND Liver cancer is a malignant tumor with high morbidity and mortality.Transcatheter arterial chemoembolization(TACE)is the main method for surgically unresectable liver cancer.In recent years,drug-loaded microspheres have been gradually applied in TACE technology.There are some controversies about the therapeutic effects of drug-loaded microspheres TACE(D-TACE)and traditional TACE.AIM To explore the short-term efficacy of D-TACE and traditional TACE in the treatment of advanced liver cancer.METHODS The clinical data of 73 patients with advanced liver cancer admitted to the First and Sixth Medical Centers of Chinese PLA General Hospital from January 2017 to October 2019 were retrospectively analyzed.Among them,15 patients were treated with D-TACE,and 58 patients were treated with traditional TACE.Clinical baseline characteristics,perioperative laboratory indices,postoperative adverse reactions and postoperative complications were compared between the two groups.RESULTS There was no statistical difference between the two groups for the postoperative response:The highest postoperative body temperature of the drug-loaded microsphere group was 38.0±0.9℃and the postoperative highest body temperature of the traditional TACE group was 38.3±0.7℃(t=-1.414,P=0.162).For the 24 h postoperative nausea and vomiting after surgery in terms of scoring and postoperative pain scores,the traditional TACE group was higher than the drugloaded microsphere group(χ2=14.33,P=0.014;χ2=32.967,P=0.000)and the two groups had significant statistical differences.The disease control rate at 3 mo after treatment in the drugloaded microsphere group was 60%and the disease control rate at 3 mo after treatment in the traditional TACE group was 75.9%(χ2=4.091,P=0.252).There was no statistical difference between the two groups of data.During the follow-up period,the number of interventional treatments received was once in the drug-loaded microsphere group and the traditional TACE group received an average of 1.48 treatments(χ2=10.444 P=0.005).There was a statistical difference between the two groups.CONCLUSION Compared with traditional TACE,D-TACE may have some advantages in the treatment of advanced hepatocellular carcinoma with a large tumor load in the short term,but the long-term clinical efficacy needs additional follow-up studies.In addition,compared with the traditional group,the patients in the drug-loaded microsphere group had better subjective tolerance and could reduce the number of interventional treatments.Therefore,D-TACE is worthy of clinical promotion.
基金This work was supported by the science and technology project of State Grid Shanghai Municipal Electric Power Company(No.52090020007F)National Key R&D Program of China(2017YFB0902800).
文摘Acoustic array sensor device for partial discharge detection is widely used in power equipment inspection with the advantages of non-contact and precise positioning compared with partial discharge detection methods such as ultrasonic method and pulse current method.However,due to the sensitivity of the acoustic array sensor and the influence of the equipment operation site interference,the acoustic array sensor device for partial discharge type diagnosis by phase resolved partial discharge(PRPD)map might occasionally presents incorrect results,thus affecting the power equipment operation and maintenance strategy.The acoustic array sensor detection device for power equipment developed in this paper applies the array design model of equal-area multi-arm spiral with machine learning fast fourier transform clean(FFT-CLEAN)sound source localization identification algorithm to avoid the interference factors in the noise acquisition system using a single microphone and conventional beam forming algorithm,improves the spatial resolution of the acoustic array sensor device,and proposes an acoustic array sensor device based on the acoustic spectrogram.The analysis and diagnosis method of discharge type of acoustic array sensor device can effectively reduce the system misjudgment caused by factors such as the resolution of the acoustic imaging device and the time domain pulse of the digital signal,and reduce the false alarm rate of the acoustic array sensor device.The proposed method is tested by selecting power cables as the object,and its effectiveness is proved by laboratory verification and field verification.
基金supported by the science and technology project of State Grid Shanghai Municipal Electric Power Company (No. 52090020007F)National Key R&D Program of China (2017YFB0902800)。
文摘The pulse current method,acoustic and ultrasonic partial discharge(PD)detection,and voiceprint PD detection are commonly used detection methods for the PD detection of power equipment.To study the characteristics of PD signals of typical discharge models based on the principles of the above three detection methods,an acoustic detection experimental system consisting of a needle-tip model and a surface model was built.Acoustic tests were carried out on needle-tip models with different curvature radii and surface discharge models with different lengths of conductive paste.The experimental results showed that acoustic and ultrasonic PD detection and voiceprint PD detection exhibited different sensitivities to the needle-tip discharge models,and the combination of acoustic and ultrasonic PD and voiceprint PD detection was more beneficial for the comprehensive detection of cable PD signals.Based on voiceprint recognition technology,this study drew FFT(Fast Fourier Transformation)diagrams of different types of PD acoustic signals and analyzed the differences in the ultrasonic signal frequency distribution.The frequency band of the voiceprint PD signal of the needle-tip discharge models was concentrated in the range 17-27 kHz,and the frequency band of the voiceprint PD signal of the conductive paste discharge models was concentrated in the range 20-25 kHz.The measurement of voiceprint PD signals in these frequency bands were strengthened when the PD of a cable was detected on-site,which provides the basis for the use of the cable model for on-site PD detection.
基金supported by the National Natural Science Foundation of China:Surface Project(81274187)Longitudinal Research Project of the Beijing University of Chinese Medicine(2020072120043).
文摘Objective:To investigate the biochemical constituents of the fruits of Terminalia bellerica(Gaertn.)Roxb.(hereafter termed T.bellerica)and estimate the anti-cancer activity of different polar extracts.Methods:To rapidly screen and identify the biochemical constituents of ethyl acetate(EA)extracts of T.bellerica,ultra performance liquid chromatography-electrospray ionization/mass spectrometry(UPLC-ESI-MSn)was done.The CellTiter-BlueTM cell-viability assay was used to ascertain the anti-cancer activity of different polar extracts in 10 human cancer cell lines.Results:Forty polyphenols of the EA extract of T.bellerica were characterized tentatively.The EA extract exhibited significant anti-cancer activity against ZR-75-1 cells(half-maximal inhibitory concentration=27.33(0.98)μg/mL)and Colo-205 cells(39.65(2.99)μg/mL)in vitro.Treatment of ZR-75-1 cells with 20 and 60μg/mL of the EA extract elicited dosedependent apoptosis percentages at an early stage of 17.58(0.74)%and at a late stage of 29.20(1.22)%;Colo-205 cells at the same concentration of EA extract had values of 21.33(1.03)%and 40.55(0.34)%,respectively.Western blotting suggested that ZR-75-1 and Colo205 cells treated with the EA extract showed a similar increasing tendency for expression of cleaved anti-poly adenosine diphosphate ribose polymerase I.Conclusion:We identified a total of 40 chemical constituents,of which 11 were first obtained from the Terminalia Linn.genus using UPLC-ESI-MSn.Meanwhile,we observe that the EA extract of T.bellerica possesses anti-cancer activity,especially against breast and colon cancers.
基金supported by the Technology Project of State Grid Jiangsu Electric Power Co.,Ltd.,China,under Grant J2020090.
文摘Virtual power plants can effectively integrate different types of distributed energy resources,which have become a new operation mode with substantial advantages such as high flexibility,adaptability,and economy.This paper proposes a distributionally robust optimal dispatch approach for virtual power plants to determine an optimal day-ahead dispatch under uncertainties of renewable energy sources.The proposed distributionally robust approach characterizes probability distributions of renewable power output by moments.In this regard,the faults of stochastic optimization and traditional robust optimization can be overcome.Firstly,a second-order cone-based ambiguity set that incorporates the first and second moments of renewable power output is constructed,and a day-ahead two-stage distributionally robust optimization model is proposed for virtual power plants participating in day-ahead electricity markets.Then,an effective solution method based on the affine policy and second-order cone duality theory is employed to reformulate the proposed model into a deterministic mixed-integer second-order cone programming problem,which improves the computational efficiency of the model.Finally,the numerical results demonstrate that the proposed method achieves a better balance between robustness and economy.They also validate that the dispatch strategy of virtual power plants can be adjusted to reduce costs according to the moment information of renewable power output.
基金Supported by Huzhou Public Welfare Application Research Project(2017GY)National Staple Freshwater Fish Industrial Technology System(CARS-45-39)。
文摘[Objectives] This study was conducted to strengthen the understanding of the variation laws of pH in fishery waters. [Methods]Based on the investigation data of pH, dissolved oxygen(Do), water temperature(T) and chlorophyll a(Chl. a) of the fishery waters in Quzhou City from 2017 to 2020, the seasonal variation characteristics and influencing factors of pH in the fishery waters were analyzed and discussed. [Results] The pH of the fishery waters in Quzhou City was in the range of 6.40-11.3, and was characterized by the highest seasonal variation in spring and the lowest in winter. The correlation analysis showed that water temperature and pH were significantly positively correlated in spring and summer(P<0.05), and the photosynthesis of phytoplankton was the main reason that affected the pH of water bodies in spring and summer. There was a negative correlation between water temperature and pH in winter, and the pH in the natural waters had a significant negative correlation with water temperature(P<0.05). The balance of carbonate system in the water bodies was the main reason that affected the pH of water in winter. The level of water eutrophication(Chl. a) affected the pH of fishery waters to a certain extent. When Chl. a<10 μg/L, there was a significant positive correlation between the pH of fishery waters and Chl. a(P<0.05). Various environmental factors had different effects on the pH of different types of fishery waters. The stepwise regression analysis showed that the pH of natural waters was mainly affected by Chl. a, and the pH of aquaculture waters was mainly affected by water temperature and dissolved oxygen. [Conclusions]This study provides certain theoretical support for actual production management.
基金Supported by Anhui Provincial Education Department Project(KJ2019A0213)National Natural Science Foundation of China(31501245)Undergraduate Innovation and Enterpreneurship Training Program of Anhui Province(S202010364185,XJDC2020541,202110364788)。
文摘[Objectives]This study was conducted to construct a pColdI-HSP70 recombinant prokaryotic expression vector.[Methods]With rice‘N22’as the test variety,hydroponic experiments were set up for rice seedlings with normal growth(CK),high temperature treatment(H),drought treatment(D)and drought-high temperature cross treatment(DH).The recombinant prokaryotic expression vector was constructed by the method of prokaryotic expression,and its induction expression time,IPTG concentration and temperature were optimized.[Results]The pColdI-HSP70 expression vector was successfully constructed,and the fusion protein was highly expressed in host strain BL21,and the expressed proteins were all in a soluble form.By optimizing the induction expression conditions,it was found that the optimal expression conditions were the IPTG concentration of 0.5 mmol/L and induction at 20℃for 36 h.The expression analysis of the rice HSP70 gene under different stress treatments was carried out by qRT-PCR technology,and it was found that H,D and DH stresses all could induce its expression,and its expression levels were 4.65,1.40 and 17.66 times higher than that of the CK group,respectively.[Conclusions]This study lays a solid foundation for the isolation,purification and functional study of rice HSP70 proteins.
基金This study is registered at https://www.clinicaltrials.gov/.The registration identification number is NCT04639323.
文摘BACKGROUND We invented Endoscopic Ruler,a new endoscopic device to measure the size of varices in patients with cirrhosis and portal hypertension.AIM To assess the feasibility and safety of Endoscopic Ruler,and evaluate the agreement on identifying large oesophageal varices(OV)between Endoscopic Ruler and the endoscopists,as well as the interobserver agreement on diagnosing large OV using Endoscopic Ruler.METHODS We prospectively and consecutively enrolled patients with cirrhosis from 11 hospitals,all of whom got esophagogastroduodenoscopy(EGD)with Endoscopic Ruler.The primary study outcome was a successful measurement of the size of varices using Endoscopic Ruler.The secondary outcomes included adverse events,operation time,the agreement of identifying large OV between the objective measurement of Endoscopic Ruler and the empirical reading of endoscopists,together with the interobserver agreement on diagnosing large OV by Endoscopic Ruler.RESULTS From November 2020 to April 2022,a total of 120 eligible patients with cirrhosis were recruited and all of them underwent EGD examinations with Endoscopic Ruler successfully without any adverse event.The median operation time of Endoscopic Ruler was 3.00 min[interquartile range(IQR):3.00 min].The kappa value between Endoscopic Ruler and the endoscopists while detecting large OV was 0.52,demonstrating a moderate agreement.The kappa value for diagnosing large OV using Endoscopic Ruler among the six independent observers was 0.77,demonstrating a substantial agreement.CONCLUSION The data demonstrates that Endoscopic Ruler is feasible and safe for measuring the size of varices in patients with cirrhosis and portal hypertension.Endoscopic Ruler is potential to promote the clinical practice of the two-grade classification system of OV.
基金supported by the grants from Canadian Institutes of Health Research(PJT-155962 and PJT-166107)to B.B.Y.
文摘Colitis is a chronic bowel disease characterized by damage to the lining of the large intestine,with its precise underlying causes remaining incompletely understood.In this study,we provide evidence that circular RNA circNlgn plays a pivotal role in promoting the development of colitis.Colitis patients produce significant higher levels of circNign.Transgenic mice expressing circNlgn exhibit heightened susceptibility to colitis development and progression,primarily attributed to the presence of the protein isoform Nlgn173 encoded by circNign.Nlgn173 undergoes translocation into cellnuclei,where it interacts with actin,impeding the binding of actin-related protein 2 and 3(Arp2/3)complex to actin molecules.Consequently,this leads to a reduction in actin polymerization.Mechanistically,Nlgn173 enhances tyrosine-53 phosphorylation of nuclear actin,diminishing its capacity to interact with the Arp2/3 complex and causing a decrease in filamentous actin levels.These alterations in actin dynamics result in inhibited cell cycle progression,increased apoptosis,and decreased proliferation of colonic epithelial cells,thereby exacerbating colitis development and progression.In contrast,the silencing of circNign or the targeted inhibition of Nlgn173 translation and nuclear translocation leads to the promotion of nuclear actin polymerization,enhanced cell survival,and reduced apoptosis and ultimately improves the outcome of colitis in vivo.Interestingly,nuclear actin polymerization is highly related with expression of PIAS3,which modulates signal transducer and activator of transcription 3 and NF-kB activity in colitis.Strategies such as circNlgn knockdown and targeting nuclear actin polymerization of the colonic epithelium may explore a novel avenue for acute ulcerativecolitisclinical intervention.
基金supported by National Key R&D Program of China(2022YFA1103900)National Natural Science Foundation of China(82430099)+1 种基金the Clinical Research Special Project of Shanghai Municipal Health Commission(202440070)the Medical Research Special Project for Shanghai Science and Technology Innovation Action Plan(24Y12800400).
文摘Recent large-scale multi-omics studies have characterized the heterogeneity of esophageal squamous cell carcinoma(ESCC),but inconsistent clinical management has hindered the identification of prognostic markers and patient stratification.Here,we conducted genomic and transcriptomic profiling of 203 patients from the ECTOP-2002 study with full clinical information.Mutation in the mucin family,as well as APOBEC signature,were associated with poor prognosis.In contrast,activation of the epithelial-keratinization(EpK)pathway was strongly linked to favorable prognosis and lower post-chemotherapy recurrence rates.Independent validation supported S100A8+S100A9 complex as a key marker of EpK pathway.Furthermore,we established a prognostic stratification system,FU-ESCC subtyping,which defines three subtypes with distinct molecular and clinical features.The EpK-activated subtype retained characteristics of healthy squamous epithelial cells,showed high expression of the S100A8+S100A9 complex,and was associated with favorable prognosis.
基金supported by the National Natural Science Foundation of China(21975107)the Natural Science Foundation of Jiangsu Province(BK2019020945)financial support from the PolyU Departmental General Research Fund(P0046213)。
文摘Self-healing polyurethane-elastomers are highly desired in various fields.However,there is often a trade-off between mechanical properties and dynamic self-healing due to the mutually exclusive mechanism.Herein,we develop a self-healable and mechanically robust poly(oxime-urethane)elastomer(Zn-DAPU)to circumvent this inherent trade-off by incorporating zincpyridinyl cross-links into the hydrogen bonding and dynamic oxime-urethane supramolecular-covalent hybrid network.Benefiting from the synergistic strengthening of H-bonding and coordinate interactions,Zn-DAPU network performs tunable toughness with metal ion concentration change,which improves 345.2%and reaches 82.2 MJ m^(-3),with robust tensile strength of 22.8 MPa,Young's modulus of 37.1 MPa,and satisfactory elongation of 815.7%.The healing efficiency can be reached at 91.7%with a restored toughness of 75.4 MJ m^(-3)at 80℃for 10 h.Furthermore,zinc-contained networks exhibit photolysis behavior due to the homolytic cleavage of N-O bonds in oxime-urethane moieties,which can be functionalized further with fluorescamine as the specific information encryption coating with quick response codes(QRs)upon polyester fabric.This work provides valuable guidance towards the development of high-performance self-healing polyurethane and wearable functional materials.
文摘Covariate-adaptive randomisation has a long history of applications in clinical trials. Shao, Yu,and Zhong [(2010). A theory for testing hypotheses under covariate-adaptive randomization.Biometrika, 97, 347–360] and Shao and Yu [(2013). Validity of tests under covariate-adaptivebiased coin randomization and generalized linear models. Biometrics, 69, 960–969] showed thatthe simple t-test is conservative under covariate-adaptive biased coin (CABC) randomisation interms of type I error, and proposed a valid test using the bootstrap. Under a general additivemodel with CABC randomisation, we construct a calibrated t-test that shares the same propertyas the bootstrap method in Shao et al. (2010), but do not need large computation required by thebootstrap method. Some simulation results are presented to show the finite sample performanceof the calibrated t-test.
